##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033076_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2510820
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.567689041826974	33.0	31.0	34.0	30.0	34.0
2	31.670497287738666	33.0	31.0	34.0	30.0	34.0
3	31.815025768474044	34.0	31.0	34.0	30.0	34.0
4	35.17104451932038	37.0	35.0	37.0	33.0	37.0
5	35.2593917524952	37.0	35.0	37.0	33.0	37.0
6	35.284244589417	37.0	35.0	37.0	33.0	37.0
7	35.21152850463195	37.0	35.0	37.0	33.0	37.0
8	35.17616595375216	37.0	35.0	37.0	33.0	37.0
9	36.81929528998494	39.0	38.0	39.0	33.0	39.0
10-11	36.81391258632638	39.0	38.0	39.0	33.0	39.0
12-13	36.73917325813878	39.0	38.0	39.0	33.0	39.0
14-15	38.125933559554255	41.0	39.0	41.0	33.0	41.0
16-17	38.13128460025012	41.0	39.0	41.0	33.0	41.0
18-19	38.14559506456058	41.0	38.5	41.0	33.5	41.0
20-21	38.16890577580233	41.0	39.0	41.0	34.0	41.0
22-23	38.13263475677269	40.0	39.0	41.0	33.0	41.0
24-25	38.14423973044663	40.0	38.5	41.0	33.0	41.0
26-27	38.052883918401164	40.0	38.0	41.0	33.0	41.0
28-29	38.06007001696656	40.0	38.0	41.0	33.0	41.0
30-31	38.20887877267188	40.5	39.0	41.0	33.0	41.0
32-33	38.19628965835862	41.0	39.0	41.0	33.0	41.0
34-35	38.1574071418899	41.0	38.0	41.0	33.0	41.0
36-37	38.0887451111589	41.0	38.0	41.0	33.0	41.0
38-39	38.00451326658224	40.0	38.0	41.0	33.0	41.0
40-41	37.94316538023435	40.0	38.0	41.0	33.0	41.0
42-43	37.83213910196669	40.0	38.0	41.0	33.0	41.0
44-45	37.71618315928661	40.0	38.0	41.0	32.0	41.0
46-47	37.59787539528919	40.0	38.0	41.0	32.0	41.0
48-49	37.4911797739384	40.0	38.0	41.0	32.0	41.0
50-51	37.38113484837623	40.0	37.5	41.0	31.5	41.0
52-53	37.236565544324165	40.0	37.0	41.0	31.0	41.0
54-55	37.092000621310966	40.0	37.0	41.0	31.0	41.0
56-57	36.92900426952151	40.0	36.5	41.0	31.0	41.0
58-59	36.74628248938594	40.0	36.0	41.0	30.5	41.0
60-61	36.5364371002302	39.5	35.5	41.0	30.0	41.0
62-63	36.29578563975116	39.0	35.0	41.0	30.0	41.0
64-65	36.030117650807306	39.0	35.0	41.0	30.0	41.0
66-67	35.740692682071995	38.5	35.0	40.0	30.0	41.0
68-69	35.42839809305326	37.5	35.0	40.0	29.0	41.0
70-71	35.09432396587569	37.0	35.0	40.0	29.0	41.0
72-73	34.73941023251368	36.5	34.0	39.0	29.0	41.0
74-75	34.380342278618144	36.0	34.0	39.0	29.0	41.0
76-77	34.00900144176006	36.0	34.0	39.0	28.0	40.0
78-79	33.612575572920406	35.0	34.0	37.5	28.0	39.5
80-81	33.25145948335604	35.0	34.0	37.0	27.0	39.0
82-83	33.00585864378968	35.0	34.0	37.0	27.0	39.0
84-85	32.76673855553166	35.0	34.0	36.0	27.0	37.5
86-87	32.5728285978286	35.0	34.0	36.0	27.0	37.0
88-89	32.34299153264671	35.0	34.0	35.5	27.0	37.0
90-91	32.12515990791853	35.0	33.0	35.0	26.5	36.0
92-93	31.90175440692682	35.0	33.0	35.0	26.0	36.0
94-95	31.698735472873402	35.0	33.0	35.0	26.0	36.0
96-97	31.52137389378769	35.0	33.0	35.0	25.0	36.0
98-99	31.34716546785512	35.0	33.0	35.0	25.0	35.5
100	31.210541177782556	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1557137583324142
1101	2	0.19901410183087265
1101	3	0.22763619251352907
1101	4	0.16933307314273094
1101	5	0.09164990999186529
1101	6	0.21495316486998206
1101	7	0.2810373640245558
1101	8	0.31115466715725404
1101	9	0.41032656063939754
1101	10-11	0.396598257937562
1101	12-13	0.379679446879166
1101	14-15	0.4438262586587882
1101	16-17	0.48680842394778523
1101	18-19	0.4296951382360632
1101	20-21	0.45368937132116827
1101	22-23	0.40607029149192897
1101	24-25	0.40343206851198943
1101	26-27	0.3189187788052692
1101	28-29	0.39137578146677043
1101	30-31	0.43307479468163734
1101	32-33	0.42101295150991547
1101	34-35	0.4209416024638415
1101	36-37	0.4356630346918635
1101	38-39	0.5243392935759061
1101	40-41	0.5285874468572018
1101	42-43	0.5094437394761044
1101	44-45	0.5174002343507169
1101	46-47	0.43313374534443483
1101	48-49	0.4534309714953295
1101	50-51	0.4269166500195354
1101	52-53	0.4133895765791493
1101	54-55	0.4037767010928306
1101	56-57	0.45012992620569037
1101	58-59	0.4410468762906419
1101	60-61	0.5295093595653739
1101	62-63	0.4686059571978376
1101	64-65	0.3822688804103933
1101	66-67	0.39493787100629874
1101	68-69	0.5254849507428929
1101	70-71	0.47773235659079916
1101	72-73	0.485603040811057
1101	74-75	0.41718206128624047
1101	76-77	0.380787481997956
1101	78-79	0.364823608081835
1101	80-81	0.45231792614941924
1101	82-83	0.41825977228040045
1101	84-85	0.35985796124450786
1101	86-87	0.3933196781492967
1101	88-89	0.4143854753876468
1101	90-91	0.4102329130180493
1101	92-93	0.47943642614167103
1101	94-95	0.4254382486936201
1101	96-97	0.434494094711841
1101	98-99	0.42931143506514147
1101	100	0.3913119280081432
1102	1	0.028168607449238436
1102	2	0.13105626582194674
1102	3	0.1456369838269076
1102	4	-0.026248891401174035
1102	5	0.12368000107486665
1102	6	0.16479270789589862
1102	7	0.1591525627912418
1102	8	0.1722440772018814
1102	9	0.2759276010610989
1102	10-11	0.19577730018881567
1102	12-13	0.14971855319708993
1102	14-15	0.2648907339645703
1102	16-17	0.24669663940633058
1102	18-19	0.17853544747327987
1102	20-21	0.1981360897527793
1102	22-23	0.19711647130448995
1102	24-25	0.16015626847691067
1102	26-27	0.04068798237072002
1102	28-29	0.04251394761765681
1102	30-31	0.18184914690694143
1102	32-33	0.2392820448481885
1102	34-35	0.16030822218789353
1102	36-37	0.18406755805362707
1102	38-39	0.2121304329493512
1102	40-41	0.10892944826530737
1102	42-43	0.1557540231177157
1102	44-45	0.12808019863403075
1102	46-47	0.08951692407794098
1102	48-49	0.10520535599474812
1102	50-51	0.09997525553546893
1102	52-53	0.08109102124689116
1102	54-55	0.11887686097774264
1102	56-57	0.14860827873017257
1102	58-59	0.10456664640334878
1102	60-61	0.03532296855033934
1102	62-63	0.09325096332565153
1102	64-65	0.08250962990744881
1102	66-67	0.07297599612295613
1102	68-69	0.058902956657078676
1102	70-71	0.03691744041896783
1102	72-73	-0.003493475343837815
1102	74-75	0.08236399078968759
1102	76-77	0.05634583691993811
1102	78-79	-0.035752536022577885
1102	80-81	-0.014012235953707375
1102	82-83	-0.019775045166809946
1102	84-85	0.07028665706135229
1102	86-87	-0.026680400401446036
1102	88-89	0.010162826373047551
1102	90-91	0.046163996350657044
1102	92-93	0.04882187926079595
1102	94-95	0.052295696337420594
1102	96-97	0.02032470139510778
1102	98-99	0.044170640538009565
1102	100	0.006404224280380788
1103	1	0.05952237261845639
1103	2	0.15642439410569509
1103	3	0.11066503455026577
1103	4	0.1889722170482031
1103	5	0.09726302432950718
1103	6	0.08010937588943534
1103	7	0.18290414005281264
1103	8	0.11584212097177016
1103	9	0.22110234837476384
1103	10-11	0.19769584880145175
1103	12-13	0.2523227099173795
1103	14-15	0.29275110707749974
1103	16-17	0.3360287750005213
1103	18-19	0.3638382506937745
1103	20-21	0.284280261732512
1103	22-23	0.3335752358193673
1103	24-25	0.2983165285867031
1103	26-27	0.18696443805821872
1103	28-29	0.23544113594072513
1103	30-31	0.24198209198363685
1103	32-33	0.1493147057263826
1103	34-35	0.20412395554311757
1103	36-37	0.29268595586587765
1103	38-39	0.40659931359812873
1103	40-41	0.33437883398998025
1103	42-43	0.2910615923281128
1103	44-45	0.2925929106823446
1103	46-47	0.2587128722342271
1103	48-49	0.200717777619424
1103	50-51	0.30042340739370843
1103	52-53	0.31304447443185524
1103	54-55	0.256292130316524
1103	56-57	0.32035554022875345
1103	58-59	0.30895812787224486
1103	60-61	0.17892364952886908
1103	62-63	0.17371062796365067
1103	64-65	0.23643638809269873
1103	66-67	0.2741888144780589
1103	68-69	0.2164776605518739
1103	70-71	0.20332906668963346
1103	72-73	0.22114106251594023
1103	74-75	0.14803722344447579
1103	76-77	0.16653320852653053
1103	78-79	0.20986178188738336
1103	80-81	0.21779009442933983
1103	82-83	0.22905050827704798
1103	84-85	0.16113336885487684
1103	86-87	0.12396704258503632
1103	88-89	0.14064641189875715
1103	90-91	0.2176161632970377
1103	92-93	0.2858790338548651
1103	94-95	0.2751579307905594
1103	96-97	0.30755388218303636
1103	98-99	0.2412875597360049
1103	100	0.22415523425275197
1104	1	0.08666492911443413
1104	2	0.1756736640307075
1104	3	0.2607350680873779
1104	4	0.1739109502678744
1104	5	0.1460076641738226
1104	6	0.2410437294110679
1104	7	0.3079072564799148
1104	8	0.3040736057737803
1104	9	0.31297596492846225
1104	10-11	0.3195715762151181
1104	12-13	0.31478940372104347
1104	14-15	0.3921713843607648
1104	16-17	0.3778294612239179
1104	18-19	0.3983819159333919
1104	20-21	0.3919312307612799
1104	22-23	0.42353736460145797
1104	24-25	0.36910604165713323
1104	26-27	0.31757138509066607
1104	28-29	0.32092103128575644
1104	30-31	0.3431517236817072
1104	32-33	0.3902949742116206
1104	34-35	0.41402970608126566
1104	36-37	0.47059963316992537
1104	38-39	0.4354168259951763
1104	40-41	0.5056697376398702
1104	42-43	0.47920783542874545
1104	44-45	0.47140435643463263
1104	46-47	0.4311374430240562
1104	48-49	0.4661975954000539
1104	50-51	0.47122925647822456
1104	52-53	0.40654405467385146
1104	54-55	0.45617742996959976
1104	56-57	0.4991948062763285
1104	58-59	0.4506092968272313
1104	60-61	0.5141379461372253
1104	62-63	0.5757016667418213
1104	64-65	0.48199109668361473
1104	66-67	0.5214569841582701
1104	68-69	0.592975573713133
1104	70-71	0.4452818582999427
1104	72-73	0.40465425689448153
1104	74-75	0.5010314589051674
1104	76-77	0.4326557028300968
1104	78-79	0.5020105383914171
1104	80-81	0.4520632181799513
1104	82-83	0.4989221732152629
1104	84-85	0.5189472429271618
1104	86-87	0.513061850906368
1104	88-89	0.5111051048545789
1104	90-91	0.5397580870099148
1104	92-93	0.44571676119872095
1104	94-95	0.5172379517741028
1104	96-97	0.5480304173271122
1104	98-99	0.6533374754401144
1104	100	0.608507691354685
1105	1	0.053247973347829713
1105	2	0.10807559882856665
1105	3	0.11463984691890161
1105	4	0.19422514492200804
1105	5	0.18201691786737229
1105	6	0.2214335713105413
1105	7	0.22878302584610566
1105	8	0.24117223699764878
1105	9	0.3120145402771115
1105	10-11	0.30516742554380727
1105	12-13	0.32532326242156273
1105	14-15	0.30704296619180127
1105	16-17	0.30428163581270695
1105	18-19	0.34068632998953774
1105	20-21	0.3398625843524101
1105	22-23	0.3795739352726386
1105	24-25	0.2351164086028774
1105	26-27	0.1986528058999184
1105	28-29	0.18143596020718888
1105	30-31	0.2514846146838394
1105	32-33	0.24581965850131127
1105	34-35	0.24425410612855103
1105	36-37	0.2938761641892711
1105	38-39	0.2798505388649062
1105	40-41	0.3410263650202907
1105	42-43	0.29757310507496726
1105	44-45	0.21800109509234744
1105	46-47	0.21188085291924352
1105	48-49	0.25471243388378184
1105	50-51	0.19577263404642764
1105	52-53	0.26511371203100254
1105	54-55	0.20617954492415436
1105	56-57	0.18754341307528222
1105	58-59	0.15789700349105118
1105	60-61	0.08398145671922208
1105	62-63	0.06669637740891687
1105	64-65	0.0413104062336771
1105	66-67	0.04953317432929083
1105	68-69	0.15776134828173838
1105	70-71	0.050556285444855575
1105	72-73	0.07238725994054107
1105	74-75	0.09886322995463104
1105	76-77	0.1332959014456634
1105	78-79	0.1265152927718276
1105	80-81	0.15736309215860445
1105	82-83	0.18492168314473645
1105	84-85	0.19630239081264733
1105	86-87	0.05718388311042588
1105	88-89	0.08219606429604198
1105	90-91	0.1002137822306679
1105	92-93	0.168259766085729
1105	94-95	0.17531235041017013
1105	96-97	0.2654795635255738
1105	98-99	0.29076213158780817
1105	100	0.29510998204886363
1106	1	-0.20878323837021995
1106	2	-0.11575062406527792
1106	3	-0.11450081577478954
1106	4	-0.14762120135447532
1106	5	-0.07362014013266105
1106	6	-0.0732195098670303
1106	7	-0.06164778239647006
1106	8	-0.013458521548983526
1106	9	-0.07483118045600179
1106	10-11	-0.08725522428107269
1106	12-13	-0.09931668089726031
1106	14-15	-0.033465376124063084
1106	16-17	-0.060346000809147426
1106	18-19	-0.09672671658621823
1106	20-21	-0.1079914254095442
1106	22-23	-0.10685079622155769
1106	24-25	-0.14231991629507945
1106	26-27	-0.06747394106024984
1106	28-29	-0.07710042614461798
1106	30-31	-0.06472458960761429
1106	32-33	-0.130474325740785
1106	34-35	-0.07865800531779854
1106	36-37	-0.018289488672380116
1106	38-39	-0.04973508499007551
1106	40-41	-0.03136039859787587
1106	42-43	-0.06454739861487013
1106	44-45	-0.06494665060573368
1106	46-47	-0.10043524891457878
1106	48-49	-0.05215459876436768
1106	50-51	-0.08990266898010191
1106	52-53	-0.06060556148146645
1106	54-55	-0.05776920338093561
1106	56-57	0.005655275578540397
1106	58-59	0.004200310066956092
1106	60-61	-0.070285140017873
1106	62-63	-0.0480077507139427
1106	64-65	-5.155664428144746E-4
1106	66-67	-0.048377974185704886
1106	68-69	0.023033823099275708
1106	70-71	-0.10235138348516415
1106	72-73	-0.08491666117380703
1106	74-75	-0.07631877854927893
1106	76-77	-0.040358412863234605
1106	78-79	-0.08360856191908539
1106	80-81	-0.08335721813406849
1106	82-83	-0.15443849024661915
1106	84-85	-0.11809809537356131
1106	86-87	-0.16913850398623254
1106	88-89	-0.19120012649236173
1106	90-91	-0.1929469363680738
1106	92-93	-0.07897158910185098
1106	94-95	-0.08384950690917847
1106	96-97	-0.047475765437837936
1106	98-99	-0.17182487251703193
1106	100	-0.12540256965258578
1107	1	-0.08152739530307329
1107	2	-0.0682117054913185
1107	3	-0.01862276026926679
1107	4	-0.023626268940823536
1107	5	-0.05131047480682582
1107	6	-0.005399683797094212
1107	7	-0.027897968623037173
1107	8	-0.014995585639830722
1107	9	-0.0015529701878591595
1107	10-11	-0.013685305375794599
1107	12-13	-0.013262077042867304
1107	14-15	-0.011386826312069331
1107	16-17	-0.0038804366037865634
1107	18-19	0.011718714025207078
1107	20-21	-0.024412384181758284
1107	22-23	0.09476462200441205
1107	24-25	-0.027606432824221372
1107	26-27	0.062042609200908316
1107	28-29	0.04402981349284829
1107	30-31	0.05511026355690518
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2216	54-55	-0.7370263854981971
2216	56-57	-0.8821744534507658
2216	58-59	-0.7885221241853344
2216	60-61	-0.775034678331977
2216	62-63	-0.7930764396005756
2216	64-65	-0.8258036947994185
2216	66-67	-0.9073255913644331
2216	68-69	-0.9426667718486073
2216	70-71	-0.9076609927045212
2216	72-73	-0.8686905227137203
2216	74-75	-0.9682982038120969
2216	76-77	-0.7926440027631045
2216	78-79	-0.7991535330619683
2216	80-81	-0.7536327334463806
2216	82-83	-0.9453658612985194
2216	84-85	-0.7871118401425932
2216	86-87	-0.7541853111520886
2216	88-89	-0.8565039535678096
2216	90-91	-0.818857664820321
2216	92-93	-1.0634763872791595
2216	94-95	-1.0095505306819241
2216	96-97	-0.9540732242776429
2216	98-99	-0.9251011876809301
2216	100	-0.8826876980996161
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28730.0
3	5809.0
4	3248.0
5	2650.0
6	2225.0
7	2296.0
8	2652.0
9	3075.0
10	3468.0
11	3801.0
12	4258.0
13	4645.0
14	5146.0
15	5402.0
16	5608.0
17	5793.0
18	5584.0
19	5557.0
20	5539.0
21	5643.0
22	6565.0
23	8933.0
24	13363.0
25	18763.0
26	20278.0
27	18062.0
28	18890.0
29	23408.0
30	28498.0
31	33992.0
32	44386.0
33	58828.0
34	83621.0
35	126137.0
36	225558.0
37	480798.0
38	818697.0
39	369985.0
40	929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83349360436872	20.027609392453886	13.550122325389921	23.588774677787473
2	15.12598546300126	20.11205171544076	41.21476618637672	23.54719663518126
3	18.01206593344409	25.618091169169745	29.97335197273907	26.396490924647093
4	11.773129983243706	16.862834348648896	39.916130842581715	31.447904825525686
5	12.42409094496758	38.2361593785204	36.253176081900854	13.086573594611165
6	30.385493132637464	38.822492979165716	18.054376834934157	12.73763705326267
7	26.90137740212225	32.53276876044173	23.01783929684046	17.548014540595553
8	24.428057638569665	37.32816213369395	20.695297240985848	17.54848298675053
9	24.360287897355835	17.473690337323404	21.59078677432652	36.57523499099424
10-11	23.3669794192482	27.45428515157483	29.335584822343403	19.843150606833564
12-13	24.022200585934073	24.724555964359133	29.40957652946496	21.843666920241837
14-15	21.312768591029545	26.87984565279494	26.28182598740128	25.525559768774237
16-17	21.03377914230979	30.278009668948425	26.782277673274518	21.905933515467268
18-19	20.922638452876697	29.24512975849072	28.72612768882121	21.106104099811372
20-21	22.16592738584001	28.53673821087263	28.206264333656634	21.091070069630728
22-23	21.764087490246496	28.288356286119853	28.30363910392874	21.643917119704913
24-25	21.639370972397032	28.378386184195193	28.23844027032308	21.743802573084693
26-27	21.42884076143393	28.27975105663272	28.327792059069683	21.963616122863666
28-29	21.449456380625424	28.303996425887686	28.439500238028387	21.807046955458503
30-31	21.701933624142242	28.83613858702584	27.98619215708783	21.47573563174409
32-33	21.322182786334768	28.476541404028303	28.568450714329842	21.632825095307084
34-35	21.434686240438385	28.218641770817648	28.55872091679952	21.78795107194445
36-37	21.839717583263166	28.595591203447086	28.034061199059728	21.530630014230024
38-39	21.58892363886675	28.721305111116557	28.199039051176133	21.490732198840558
40-41	21.353682321564023	28.442591291225096	28.50961651527031	21.69410987194057
42-43	21.327162222245693	28.307368045966065	28.493545663239328	21.871924068548918
44-45	21.50517941625448	28.51578536212071	28.372280258432742	21.606754963192067
46-47	21.47586789673927	28.73299731544427	28.23863647687436	21.552498310942106
48-49	21.389343661969022	28.49896318380793	28.34748139859081	21.76421175563224
50-51	21.44381081591674	28.40901934869825	28.3319768864642	21.81519294892081
52-53	21.461442924878753	28.409636239569153	28.377530587061123	21.751390248490974
54-55	21.582435906786408	28.85010635207019	28.15352719420641	21.413930546936992
56-57	21.449592416593166	28.540921947966908	28.198482537463278	21.81100309797665
58-59	21.18314035456548	28.201217355435304	28.663708799174216	21.951933490825
60-61	21.602537712384358	28.622216182200706	28.161808456836052	21.613437648578884
62-63	21.41097935887546	28.56641243309953	28.23700416632706	21.785604041697944
64-65	21.35043809070607	28.351081541723783	28.436549532277468	21.861930835292682
66-67	21.256681721297493	28.393170575397235	28.257961869750076	22.092185833555195
68-69	21.69074590746565	28.550704103823204	28.16086491174266	21.59768507696848
70-71	21.680370432067132	28.347227059809043	28.19672608064605	21.775676427477777
72-73	21.36638423083077	28.47166831088253	28.424224829100847	21.737722629185853
74-75	21.382233425969485	28.511789938107736	28.357024396843794	21.748952239078985
76-77	21.503835893152498	28.488164153073885	28.156689107051736	21.851310846721884
78-79	21.615664752990757	28.601451853988934	28.255683147232435	21.527200245787874
80-81	21.54669164766826	28.35698977602569	28.363265575288573	21.733053001017478
82-83	21.371982035418704	28.43322766127906	28.510434476120384	21.684355827181857
84-85	21.374219019418504	28.382300117681254	28.377040620254125	21.866440242646117
86-87	21.60069662893592	28.479389401789224	28.17502264123729	21.74489132803756
88-89	21.350394156494744	28.386619020040634	28.35952443423616	21.903462389228466
90-91	21.327181805033554	28.275382026800944	28.457186661922666	21.940249506242836
92-93	21.530509301169182	28.493138076780866	28.220087561040913	21.75626506100904
94-95	21.280921350150113	28.232305825774617	28.462523831627685	22.02424899244759
96-97	21.36718166332965	28.24841367082051	28.450706750739624	21.93369791511022
98-99	21.343220246123565	28.356984757233388	28.361825944380136	21.937969052262908
100	21.422318105871117	28.22500530977401	28.248954084102834	22.10372250025204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	343.0
1	331.5
2	317.5
3	303.5
4	304.5
5	313.0
6	284.0
7	266.0
8	251.5
9	216.5
10	201.0
11	203.0
12	218.5
13	247.0
14	248.5
15	302.0
16	428.5
17	559.5
18	765.5
19	1125.0
20	1645.0
21	2407.5
22	3724.5
23	5882.0
24	8525.0
25	11565.0
26	15762.5
27	21695.5
28	29115.0
29	36518.5
30	43948.5
31	51955.0
32	59628.0
33	67037.5
34	74477.5
35	80625.5
36	85451.0
37	90172.0
38	93543.0
39	94459.0
40	95098.0
41	95318.5
42	93913.5
43	92272.5
44	90141.5
45	87943.0
46	86901.0
47	84861.5
48	82037.5
49	79353.5
50	76557.0
51	73433.5
52	67991.0
53	63290.5
54	60065.0
55	56331.0
56	51333.5
57	46186.0
58	41604.0
59	37268.0
60	32763.0
61	27294.5
62	22858.0
63	19174.0
64	15504.0
65	12181.0
66	9312.0
67	6766.5
68	4935.0
69	3806.0
70	2712.5
71	1900.5
72	1387.0
73	968.5
74	695.5
75	465.5
76	296.5
77	215.0
78	148.0
79	88.5
80	67.5
81	47.0
82	26.5
83	19.0
84	18.5
85	13.0
86	10.0
87	9.0
88	6.5
89	5.0
90	2.5
91	2.5
92	5.0
93	4.0
94	2.0
95	1.0
96	1.5
97	2.5
98	1.0
99	1.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06089644020678503
2	0.04325280187349152
3	0.057391609115747054
4	0.07615042097800719
5	0.08865629555284728
6	0.08821819166646752
7	0.08989095195991748
8	0.10231717128268852
9	0.0966218207597518
10-11	0.10171975689217069
12-13	0.1070566587807967
14-15	0.10787312511450443
16-17	0.07409929823722927
18-19	0.09751794234552856
20-21	0.09164336750543647
22-23	0.05838729976661011
24-25	0.022482694896488
26-27	0.18430233947475327
28-29	0.06633291116049737
30-31	0.07790283652352618
32-33	0.07268541751300372
34-35	0.02079002079002079
36-37	0.0532495360081567
38-39	0.0774846464501637
40-41	0.08216439250922009
42-43	0.07668809392947325
44-45	0.07368110816386678
46-47	0.08065094271990823
48-49	0.07716602544188751
50-51	0.07224731362662397
52-53	0.07630973148214527
54-55	0.06774679188472292
56-57	0.07021610469886333
58-59	0.06740825706342948
60-61	0.06495885806230633
62-63	0.07071395002429484
64-65	0.067448084689464
66-67	0.0691407587959312
68-69	0.06804549907998184
70-71	0.061095578336957654
72-73	0.06047825013342254
74-75	0.06135445790618205
76-77	0.05697341904238455
78-79	0.05332919126022574
80-81	0.04697668490771939
82-83	0.052831345934794216
84-85	0.04279478417409452
86-87	0.04058435092917852
88-89	0.043551509068750445
90-91	0.0391505563919357
92-93	0.04094279956348922
94-95	0.04082331668538565
96-97	0.043611250507802235
98-99	0.044009526768147456
100	0.051656430966775795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2510820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.2797747366466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90867280290323	55.36054851289535
2	12.738971651856385	17.651061728342746
3	3.6310130405240266	7.546672965399925
4	1.4609839022619446	4.0486654257029775
5	0.7165303268125869	2.48205298167759
6	0.4108159786167915	1.7076743074071805
7	0.24461861578507169	1.1862985818586052
8	0.1696594664073463	0.9403175691712493
9	0.13032040576415063	0.8125711519435863
>10	0.5577313394730571	6.498112924072362
>50	0.02468100634466657	1.132976508294316
>100	0.005944050624187565	0.6113300084940398
>500	5.741262656072278E-5	0.02171733474000684
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.9827626034522585E-5	0.0
10-11	0.0	0.0	0.0	1.1948287810356776E-4	0.0
12-13	0.0	0.0	0.0	1.5931050413809034E-4	0.0
14-15	0.0	0.0	0.0	1.5931050413809034E-4	0.0
16-17	0.0	0.0	0.0	1.5931050413809034E-4	0.0
18-19	0.0	0.0	0.0	1.5931050413809034E-4	0.0
20-21	0.0	0.0	0.0	2.1905194318987424E-4	0.0
22-23	0.0	0.0	0.0	2.787933822416581E-4	0.0
24-25	0.0	0.0	0.0	4.580176993970097E-4	0.0
26-27	0.0	0.0	0.0	6.571558295696226E-4	0.0
28-29	0.0	0.0	0.0	0.001155001155001155	0.0
30-31	0.0	0.0	0.0	0.003066727204658239	0.0
32-33	0.0	0.0	0.0	0.007507507507507507	0.0
34-35	0.0	0.0	0.0	0.013601134290789463	0.0
36-37	0.0	0.0	0.0	0.024573645263300435	0.0
38-39	0.0	0.0	0.0	0.045284010801252175	0.0
40-41	0.0	0.0	0.0	0.0731036075863662	0.0
42-43	0.0	0.0	0.0	0.10211803315251591	0.0
44-45	0.0	0.0	0.0	0.13326323671151258	0.0
46-47	0.0	0.0	0.0	0.17099991237922274	0.0
48-49	0.0	0.0	0.0	0.21447176619590413	0.0
50-51	0.0	0.0	0.0	0.26405716060888473	0.0
52-53	0.0	0.0	0.0	0.31746600712117956	0.0
54-55	0.0	0.0	0.0	0.3728064934961487	0.0
56-57	0.0	0.0	0.0	0.4368294023466437	0.0
58-59	0.0	0.0	0.0	0.5129798233246509	0.0
60-61	0.0	0.0	0.0	0.5954628368421472	0.0
62-63	0.0	0.0	0.0	0.6830238726790452	0.0
64-65	0.0	0.0	0.0	0.7752447407619821	0.0
66-67	0.0	3.9827626034522585E-5	0.0	0.8819628647214854	0.0
68-69	0.0	3.9827626034522585E-5	0.0	1.00234186441083	0.0
70-71	0.0	3.9827626034522585E-5	0.0	1.1397272604169155	0.0
72-73	0.0	3.9827626034522585E-5	0.0	1.286392493289045	0.0
74-75	0.0	3.9827626034522585E-5	0.0	1.430827379103241	0.0
76-77	0.0	3.9827626034522585E-5	0.0	1.5811169259445121	0.0
78-79	0.0	3.9827626034522585E-5	0.0	1.7566571876916703	0.0
80-81	0.0	3.9827626034522585E-5	0.0	1.9564923013198876	0.0
82-83	0.0	3.9827626034522585E-5	0.0	2.1727563106873453	0.0
84-85	0.0	3.9827626034522585E-5	0.0	2.385754454719972	0.0
86-87	0.0	3.9827626034522585E-5	0.0	2.602834930421137	0.0
88	0.0	3.9827626034522585E-5	0.0	2.7839112321870942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2980	0.0	32.18237	4
GTATCAA	3175	0.0	31.840277	1
ATCAACG	3085	0.0	31.087025	3
CAACGCA	3120	0.0	30.73829	5
GGTATCA	1995	0.0	30.639559	1
AACGCAG	3475	0.0	27.58987	6
TATCAAC	3805	0.0	26.563663	2
GTACATG	6500	0.0	25.607782	1
CTGTCGC	680	0.0	24.222214	9
TACATGG	6600	0.0	23.505754	2
TACACCG	395	0.0	22.613129	5
ACGCAGA	4105	0.0	22.439707	7
CGCAGAG	4150	0.0	22.106052	8
GTCTCGC	585	0.0	21.701513	1
ACATGGG	7095	0.0	21.534517	3
GAGTACA	4975	0.0	20.320177	1
CCTACAC	1025	0.0	19.7219	3
CATGGGG	4595	0.0	18.415823	4
CTACACT	1315	0.0	18.2326	4
AGTACAT	5105	0.0	17.404846	2
>>END_MODULE