FastQCFastQC Report
Wed 25 May 2016
SRR1033076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510820
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.17109948144430903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.1446141101313515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.048.0761341
GTATCAA68450.042.3467061
TCAACGC81100.035.001524
ATCAACG82100.034.5793233
CAACGCA83250.034.0975765
TATCAAC86900.032.960492
AACGCAG87850.032.3656586
CCGATCG600.0041631831.3313859
ACGCAGA101850.027.7321957
CGCAGAG102600.027.346258
GTACATG63250.024.5162091
GCAGAGT114150.024.2910869
TACATGG62600.023.630082
CCGGTCG1000.001759894523.4985399
CGCGCAC2602.7284841E-1123.49853794
CGACGCG1455.456968E-1222.68869490-91
GTGGTAT20000.022.387811
ACACCGT5050.022.3352436
TACACCG3650.021.8890485
AGAGTAC100700.021.39836910-11
Produced by FastQC (version 0.11.5)