##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033076_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2510820 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.687507666818014 34.0 31.0 34.0 31.0 34.0 2 32.69338502959192 34.0 31.0 34.0 31.0 34.0 3 32.80277598553461 34.0 31.0 34.0 31.0 34.0 4 36.29502313985073 37.0 37.0 37.0 35.0 37.0 5 36.27599150875013 37.0 37.0 37.0 35.0 37.0 6 36.34630678423782 37.0 37.0 37.0 35.0 37.0 7 36.29886172644793 37.0 37.0 37.0 35.0 37.0 8 36.305413370930616 37.0 37.0 37.0 35.0 37.0 9 38.09943524426283 39.0 39.0 39.0 37.0 39.0 10-11 38.070924239889756 39.0 39.0 39.0 35.0 39.0 12-13 38.041669653738616 39.0 39.0 39.0 35.0 39.0 14-15 39.538362765948975 41.0 39.0 41.0 37.0 41.0 16-17 39.53644187954533 41.0 39.0 41.0 37.0 41.0 18-19 39.5542569758087 41.0 39.5 41.0 37.0 41.0 20-21 39.54798213332696 41.0 40.0 41.0 37.0 41.0 22-23 39.47648576958922 41.0 39.0 41.0 36.5 41.0 24-25 39.424860205032616 41.0 39.0 41.0 36.0 41.0 26-27 39.31790630152699 41.0 39.0 41.0 36.0 41.0 28-29 39.266748910714426 41.0 39.0 41.0 36.0 41.0 30-31 39.264528321424876 41.0 39.0 41.0 36.0 41.0 32-33 39.22840944392669 41.0 39.0 41.0 35.5 41.0 34-35 39.147911041014495 41.0 39.0 41.0 35.0 41.0 36-37 39.06363598346357 41.0 39.0 41.0 35.0 41.0 38-39 38.976384408280964 41.0 39.0 41.0 35.0 41.0 40-41 38.8798159565401 41.0 39.0 41.0 35.0 41.0 42-43 38.76645737249186 41.0 39.0 41.0 35.0 41.0 44-45 38.60635848049641 41.0 38.5 41.0 35.0 41.0 46-47 38.40998259532742 41.0 38.0 41.0 34.5 41.0 48-49 38.206828048207356 40.0 38.0 41.0 34.0 41.0 50-51 38.078328195569576 40.0 38.0 41.0 34.0 41.0 52-53 37.95055858245513 40.0 38.0 41.0 34.0 41.0 54-55 37.809066559928624 40.0 38.0 41.0 33.0 41.0 56-57 37.67064484909312 40.0 37.0 41.0 33.0 41.0 58-59 37.50343334050231 40.0 37.0 41.0 33.0 41.0 60-61 37.317136831792006 40.0 36.5 41.0 33.0 41.0 62-63 37.09620681689647 39.5 36.0 41.0 32.5 41.0 64-65 36.83764845747605 39.0 35.5 41.0 32.0 41.0 66-67 36.56708406018751 39.0 35.0 41.0 32.0 41.0 68-69 36.26394683808477 38.5 35.0 40.5 31.5 41.0 70-71 35.94072414589656 37.0 35.0 40.0 31.0 41.0 72-73 35.59519260639951 37.0 35.0 39.5 31.0 41.0 74-75 35.24850726057623 36.5 35.0 39.0 31.0 41.0 76-77 34.887465051258154 36.0 35.0 39.0 31.0 40.5 78-79 34.51936180212043 35.5 35.0 37.5 30.0 39.5 80-81 34.17756430170223 35.0 34.0 37.0 30.0 39.0 82-83 33.86337869699939 35.0 34.0 37.0 30.0 39.0 84-85 33.62514955273576 35.0 34.0 36.0 30.0 38.0 86-87 33.3998825085032 35.0 34.0 36.0 30.0 37.0 88-89 33.17655367569161 35.0 34.0 36.0 30.0 37.0 90-91 32.984119928947514 35.0 34.0 35.0 30.0 36.5 92-93 32.80147820234027 35.0 34.0 35.0 29.0 36.0 94-95 32.639766888904816 35.0 34.0 35.0 29.0 36.0 96-97 32.488110059661786 35.0 34.0 35.0 29.0 36.0 98-99 32.35747803506424 35.0 34.0 35.0 29.0 36.0 100 32.24881313674417 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16827013488250486 1101 2 0.1183989591727439 1101 3 0.16967655762064737 1101 4 0.1540390617046512 1101 5 0.08747909746791294 1101 6 0.11449927728905607 1101 7 0.1458798642137893 1101 8 0.21089885741255188 1101 9 0.2846041240825983 1101 10-11 0.28859804635151676 1101 12-13 0.2812173268300384 1101 14-15 0.368247149227642 1101 16-17 0.36495008609232116 1101 18-19 0.3477560573797618 1101 20-21 0.3366877766861549 1101 22-23 0.380249350330196 1101 24-25 0.4006216142408192 1101 26-27 0.4025037416287205 1101 28-29 0.4030299547856373 1101 30-31 0.45021673209036805 1101 32-33 0.39936288987264845 1101 34-35 0.41834557405838524 1101 36-37 0.4225061542687527 1101 38-39 0.42426114488609556 1101 40-41 0.40485988667845163 1101 42-43 0.36350781228701123 1101 44-45 0.42898958325515224 1101 46-47 0.38205648446911766 1101 48-49 0.33089966904166346 1101 50-51 0.4375051672930752 1101 52-53 0.4015381182302349 1101 54-55 0.42862555731829843 1101 56-57 0.3884046649821755 1101 58-59 0.3712749262095514 1101 60-61 0.3898605083032578 1101 62-63 0.32629186615642425 1101 64-65 0.35987736236343437 1101 66-67 0.3417109851763058 1101 68-69 0.38452785465606354 1101 70-71 0.35739880597786566 1101 72-73 0.3429876823943019 1101 74-75 0.36369259228114714 1101 76-77 0.382346881603425 1101 78-79 0.3096271998978608 1101 80-81 0.3168386814882922 1101 82-83 0.28314411948072404 1101 84-85 0.30036077611620016 1101 86-87 0.314659898253133 1101 88-89 0.40537504815072367 1101 90-91 0.4032236223806791 1101 92-93 0.3836515011195374 1101 94-95 0.38746053619149734 1101 96-97 0.402303264236167 1101 98-99 0.3912777606053268 1101 100 0.3684251770806668 1102 1 0.1120359976505938 1102 2 0.12688892866943746 1102 3 0.10325575478763227 1102 4 0.062317149103279235 1102 5 0.09083833717865986 1102 6 0.14679157417604216 1102 7 0.10506464066946819 1102 8 0.11032265354126736 1102 9 0.181302380102359 1102 10-11 0.20022192591478216 1102 12-13 0.17342999375251367 1102 14-15 0.2685620267217814 1102 16-17 0.2652095938489438 1102 18-19 0.3007484309561832 1102 20-21 0.2713697153253847 1102 22-23 0.2926439237031673 1102 24-25 0.3168289298083593 1102 26-27 0.3307955771154525 1102 28-29 0.33445301936784233 1102 30-31 0.3061118033213788 1102 32-33 0.26942706412415873 1102 34-35 0.31123484465836526 1102 36-37 0.3493379704627344 1102 38-39 0.30576768671172516 1102 40-41 0.33827280387043146 1102 42-43 0.33596045002958874 1102 44-45 0.35046581815398525 1102 46-47 0.3466728742187897 1102 48-49 0.2538424975891189 1102 50-51 0.3286047189220156 1102 52-53 0.34178106544055 1102 54-55 0.3425493514832496 1102 56-57 0.3743333661780355 1102 58-59 0.3226954737526455 1102 60-61 0.3553454387696249 1102 62-63 0.3247775870823659 1102 64-65 0.3289012600616701 1102 66-67 0.299940003973326 1102 68-69 0.3564530924471043 1102 70-71 0.34322598887320055 1102 72-73 0.3480234682356027 1102 74-75 0.36928348428804725 1102 76-77 0.3851210036408901 1102 78-79 0.3513709444836266 1102 80-81 0.36679127782096543 1102 82-83 0.3736438849384385 1102 84-85 0.30250819981397825 1102 86-87 0.36686694367442385 1102 88-89 0.3654371169007291 1102 90-91 0.30820415801822065 1102 92-93 0.36568758596595075 1102 94-95 0.3561985347902237 1102 96-97 0.34708238893155396 1102 98-99 0.3035621067907499 1102 100 0.2733945704657046 1103 1 0.09861731796289774 1103 2 0.1196356955467266 1103 3 0.13733493867159297 1103 4 0.06551098236704433 1103 5 0.11503609045878704 1103 6 0.11130904808069886 1103 7 0.09027461249267077 1103 8 0.11191721404631494 1103 9 0.09970747568546301 1103 10-11 0.20392302915256977 1103 12-13 0.16875953033212454 1103 14-15 0.24660862892647373 1103 16-17 0.21263650906797693 1103 18-19 0.19053898616135 1103 20-21 0.22844703671174926 1103 22-23 0.2197927713132657 1103 24-25 0.18964383918056882 1103 26-27 0.1924184706657286 1103 28-29 0.2475814655650197 1103 30-31 0.19805561044040587 1103 32-33 0.1404531772018771 1103 34-35 0.2018349154413741 1103 36-37 0.16266758028896078 1103 38-39 0.19300639121232877 1103 40-41 0.12606497496525293 1103 42-43 0.15242362587527225 1103 44-45 0.19549934666556368 1103 46-47 0.19573434622928687 1103 48-49 0.1378985294267565 1103 50-51 0.1570917473690585 1103 52-53 0.11475547219881577 1103 54-55 0.15095556560655865 1103 56-57 0.15730771258638754 1103 58-59 0.12880669633197783 1103 60-61 0.14080740042460604 1103 62-63 0.12861398987026007 1103 64-65 0.11457221430048037 1103 66-67 0.1021752325668146 1103 68-69 0.12288763515140744 1103 70-71 0.10981814406210333 1103 72-73 0.051571497633119634 1103 74-75 0.11684937792189487 1103 76-77 0.15892493238732186 1103 78-79 0.13913847958557568 1103 80-81 0.175115440860651 1103 82-83 0.13288732186447305 1103 84-85 0.13599484673381568 1103 86-87 0.16017265824098104 1103 88-89 0.19686901454264216 1103 90-91 0.13760209816812363 1103 92-93 0.18675777514970804 1103 94-95 0.21615709341268996 1103 96-97 0.2597779691600266 1103 98-99 0.2229756246592487 1103 100 0.16152021204523948 1104 1 0.10308827290185008 1104 2 0.10111838036114307 1104 3 0.11318288583851199 1104 4 0.11184590183975729 1104 5 0.10653756406381376 1104 6 0.13341379628090522 1104 7 0.13088519951198663 1104 8 0.1353545662633593 1104 9 0.2087183149948828 1104 10-11 0.16996703302414318 1104 12-13 0.17680798529264763 1104 14-15 0.262817136618132 1104 16-17 0.27336287125530134 1104 18-19 0.23325113332541036 1104 20-21 0.254875961080991 1104 22-23 0.2351751784866778 1104 24-25 0.23999378525351034 1104 26-27 0.23324338872248518 1104 28-29 0.1779594155551365 1104 30-31 0.22939056275926362 1104 32-33 0.20296138379325157 1104 34-35 0.19076330853399526 1104 36-37 0.21689155194913923 1104 38-39 0.25707479003996525 1104 40-41 0.21473514189730736 1104 42-43 0.29428671187338296 1104 44-45 0.2762503854152314 1104 46-47 0.2581319839656686 1104 48-49 0.1840052670748875 1104 50-51 0.29206814285763016 1104 52-53 0.3273091370735557 1104 54-55 0.21291124451239085 1104 56-57 0.22586685319174649 1104 58-59 0.2505038673126663 1104 60-61 0.24550170128475912 1104 62-63 0.22117108340842861 1104 64-65 0.17543981494287664 1104 66-67 0.17783820445027487 1104 68-69 0.25572127429693126 1104 70-71 0.25616469028126687 1104 72-73 0.19822339434804093 1104 74-75 0.19260162802184055 1104 76-77 0.1469155480437223 1104 78-79 0.15970017095188638 1104 80-81 0.15552405649346213 1104 82-83 0.1542347485979647 1104 84-85 0.14873040805991877 1104 86-87 0.13843214616866817 1104 88-89 0.13374943420724605 1104 90-91 0.18946122600701898 1104 92-93 0.14183583383287868 1104 94-95 0.11831750753064085 1104 96-97 0.19472505638302096 1104 98-99 0.25065460235401815 1104 100 0.2769883674631046 1105 1 0.04557970134665368 1105 2 0.0875486073365721 1105 3 0.09186406905414657 1105 4 0.07026230172892411 1105 5 0.05295165300015725 1105 6 0.09444749922344897 1105 7 0.13117732527040005 1105 8 0.10440840147703057 1105 9 0.12098109102359444 1105 10-11 0.1705348140984171 1105 12-13 0.14994607849903474 1105 14-15 0.23894693598343508 1105 16-17 0.23157747079033442 1105 18-19 0.21373066427116072 1105 20-21 0.19724172753566194 1105 22-23 0.21635878341885473 1105 24-25 0.23384293591396244 1105 26-27 0.2551629274662446 1105 28-29 0.1944748149322919 1105 30-31 0.21380611771473212 1105 32-33 0.2149507429889752 1105 34-35 0.18875267925902506 1105 36-37 0.2252818199838842 1105 38-39 0.24799931416209375 1105 40-41 0.2560610170149715 1105 42-43 0.19267123097501582 1105 44-45 0.23838757057617244 1105 46-47 0.2686791687515324 1105 48-49 0.37604299097651506 1105 50-51 0.2539222235707328 1105 52-53 0.263280553668622 1105 54-55 0.2494025206804622 1105 56-57 0.28131048044436824 1105 58-59 0.1887396702965276 1105 60-61 0.268660603397997 1105 62-63 0.2942463194167644 1105 64-65 0.24067848924892843 1105 66-67 0.20118389635522504 1105 68-69 0.25141586318008535 1105 70-71 0.3035773297438027 1105 72-73 0.2503922109939012 1105 74-75 0.30677638286117315 1105 76-77 0.25851178023533805 1105 78-79 0.25262723137242915 1105 80-81 0.276724540634639 1105 82-83 0.32518128471790675 1105 84-85 0.2649547597439934 1105 86-87 0.23924027591922936 1105 88-89 0.29181503612967674 1105 90-91 0.2917704494323843 1105 92-93 0.3389534165468788 1105 94-95 0.313008956623392 1105 96-97 0.294719546271331 1105 98-99 0.2711247047200658 1105 100 0.38782972932613546 1106 1 -0.0021042243318447618 1106 2 0.0076086537078694505 1106 3 0.04092796946763855 1106 4 0.01953826424034588 1106 5 0.027448090588599428 1106 6 0.025511968418044262 1106 7 0.04521564791060939 1106 8 0.04080417987018592 1106 9 -0.01961933793698023 1106 10-11 0.07077827644621237 1106 12-13 0.0680003678441139 1106 14-15 0.054002577486848224 1106 16-17 0.02511029357216188 1106 18-19 0.07751874060367214 1106 20-21 0.11117257110984724 1106 22-23 0.15624591193731874 1106 24-25 0.07276157270437267 1106 26-27 0.06267802014802726 1106 28-29 0.05623693440065125 1106 30-31 0.05109881235284064 1106 32-33 -0.018567456268598903 1106 34-35 0.030218559053913907 1106 36-37 0.03486696164918612 1106 38-39 0.0011083877844910717 1106 40-41 0.012318043517737465 1106 42-43 0.006143646829293914 1106 44-45 -0.048023397515898125 1106 46-47 -0.05106797561403198 1106 48-49 -0.03541865771254038 1106 50-51 -0.013692831520522475 1106 52-53 0.014170939356652923 1106 54-55 0.05144881494417319 1106 56-57 -0.0263415946509582 1106 58-59 -0.011517504772790232 1106 60-61 -0.04974477216259743 1106 62-63 -0.0011873877559551715 1106 64-65 -0.05958348599693153 1106 66-67 -0.04510679075203683 1106 68-69 -0.138934022629293 1106 70-71 -0.11170712017478479 1106 72-73 -0.16713018548590952 1106 74-75 -0.164978415432941 1106 76-77 -0.1465665748636269 1106 78-79 -0.13407654748872488 1106 80-81 -0.17371743761150782 1106 82-83 -0.143982757659181 1106 84-85 -0.16539529403535624 1106 86-87 -0.23857827480104277 1106 88-89 -0.12606234173153297 1106 90-91 -0.13683575740584075 1106 92-93 -0.08191864158926165 1106 94-95 -0.19196233933450202 1106 96-97 -0.14763532911521793 1106 98-99 -0.152657795298758 1106 100 -0.1497093888546459 1107 1 -0.07587440628910258 1107 2 -0.06661458692354216 1107 3 -0.05099263241663721 1107 4 -0.031152052645012418 1107 5 -0.010595774385464551 1107 6 0.006772850164729505 1107 7 -0.005938845653176372 1107 8 0.014305806434208534 1107 9 0.001070712116273853 1107 10-11 -8.048550953461131E-4 1107 12-13 -0.012667562939633115 1107 14-15 -0.023774255260562427 1107 16-17 -0.04831717590335671 1107 18-19 0.03155024013337737 1107 20-21 -0.026394526045940836 1107 22-23 -0.009915024509943748 1107 24-25 0.010189583626704746 1107 26-27 0.03608153032433847 1107 28-29 0.05952133183817665 1107 30-31 0.05609885682486038 1107 32-33 0.024601947650722877 1107 34-35 -0.052879628489030495 1107 36-37 -0.0933168178496615 1107 38-39 -0.029982138341708264 1107 40-41 -0.025438976946063008 1107 42-43 -0.0057930594841764105 1107 44-45 -0.0032409101515114003 1107 46-47 0.0022995567004500117 1107 48-49 -0.08080068345739022 1107 50-51 -0.06419108488205438 1107 52-53 -0.05368363047664815 1107 54-55 -0.04437448439030334 1107 56-57 -0.059698942873524175 1107 58-59 -0.0017902238586202657 1107 60-61 0.022000960191462582 1107 62-63 0.058902367892542884 1107 64-65 0.07453299955744797 1107 66-67 -0.0570603130469749 1107 68-69 -0.0411149025526214 1107 70-71 -0.07407809573518875 1107 72-73 -0.044279017761915895 1107 74-75 -0.0776510780393096 1107 76-77 -0.0013051238751131677 1107 78-79 0.017788025213015146 1107 80-81 -0.06567461772985439 1107 82-83 -0.14327009358523668 1107 84-85 -0.0503966337548718 1107 86-87 -0.09184757954776046 1107 88-89 -0.11518254069933676 1107 90-91 -0.1247117127203623 1107 92-93 -0.1181690334989085 1107 94-95 -0.11165407598900856 1107 96-97 -0.16639862902782454 1107 98-99 -0.17158883810085257 1107 100 -0.15119197472454715 1108 1 -0.0359706212929396 1108 2 -0.01756127507186278 1108 3 0.0766060163046518 1108 4 -0.008221617311640728 1108 5 -0.020039636015120266 1108 6 0.024684824373977676 1108 7 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-0.2665215272459065 2214 52-53 -0.32264813926287417 2214 54-55 -0.20664176040821047 2214 56-57 -0.20706800110068002 2214 58-59 -0.23814475169118055 2214 60-61 -0.3056239284233939 2214 62-63 -0.3259681508349388 2214 64-65 -0.3252559141540843 2214 66-67 -0.30829692864656977 2214 68-69 -0.28617430561739354 2214 70-71 -0.26808239582354787 2214 72-73 -0.2653864500545282 2214 74-75 -0.4018709479761924 2214 76-77 -0.38540310244881937 2214 78-79 -0.277009743001706 2214 80-81 -0.3289855825328516 2214 82-83 -0.32734639340706195 2214 84-85 -0.2780765521451585 2214 86-87 -0.23894137158847428 2214 88-89 -0.16097515012189945 2214 90-91 -0.16294274299131217 2214 92-93 -0.19461320437815743 2214 94-95 -0.2413321934474837 2214 96-97 -0.2434028929849319 2214 98-99 -0.24959369141969745 2214 100 -0.31135712739786214 2215 1 -0.17461513873904977 2215 2 -0.10989355339351192 2215 3 -0.14697097686999427 2215 4 -0.08498312362869598 2215 5 -0.0955344121670123 2215 6 -0.1083152498553801 2215 7 -0.10925972207271428 2215 8 -0.1293793051702039 2215 9 -0.2350359945120033 2215 10-11 -0.17314890013091855 2215 12-13 -0.19950391101192366 2215 14-15 -0.25614051961659356 2215 16-17 -0.24614478133631223 2215 18-19 -0.2657767051453277 2215 20-21 -0.2520258179792094 2215 22-23 -0.2876720753096933 2215 24-25 -0.35054144652483643 2215 26-27 -0.3123565882464163 2215 28-29 -0.306121473931384 2215 30-31 -0.3662109886969773 2215 32-33 -0.3359689665955514 2215 34-35 -0.36655653221014006 2215 36-37 -0.3431289796112864 2215 38-39 -0.31050598162088505 2215 40-41 -0.2723265621080344 2215 42-43 -0.29531318848889043 2215 44-45 -0.2730452785694055 2215 46-47 -0.30187584120571387 2215 48-49 -0.26296517068231395 2215 50-51 -0.28709315198356933 2215 52-53 -0.14576970754369967 2215 54-55 -0.20158835364038197 2215 56-57 -0.24658063121023588 2215 58-59 -0.22627971838981153 2215 60-61 -0.13586218164532227 2215 62-63 -0.09611340533773216 2215 64-65 -0.20121457072255566 2215 66-67 -0.0889697548072732 2215 68-69 -0.04518412651383841 2215 70-71 -0.048514477385182886 2215 72-73 -0.133832517201256 2215 74-75 -0.0948360746089989 2215 76-77 -0.1561242499013673 2215 78-79 -0.11510998976429221 2215 80-81 -0.08898676809164385 2215 82-83 -0.1428260180809957 2215 84-85 -0.12462616016414785 2215 86-87 -0.1092942542150439 2215 88-89 -0.019219223305050548 2215 90-91 -0.08361555612389537 2215 92-93 -0.08479989431103974 2215 94-95 -0.03631565625526889 2215 96-97 0.04308375614989757 2215 98-99 0.05897832456239627 2215 100 0.0581650122361097 2216 1 -0.1338683538779719 2216 2 -0.15300000724921148 2216 3 -0.13382969787426902 2216 4 -0.10858295149678554 2216 5 -0.15024166674314188 2216 6 -0.18123649969836464 2216 7 -0.13536925987806114 2216 8 -0.19933203261812338 2216 9 -0.21587429855500773 2216 10-11 -0.22930840910117922 2216 12-13 -0.2648190554050913 2216 14-15 -0.2801864634277038 2216 16-17 -0.25542984396874857 2216 18-19 -0.28298639643849555 2216 20-21 -0.29595602460992154 2216 22-23 -0.2878595057986857 2216 24-25 -0.3492870984512777 2216 26-27 -0.36202103336092506 2216 28-29 -0.28745914203831546 2216 30-31 -0.2780557314660683 2216 32-33 -0.26736354212306424 2216 34-35 -0.27217894481250227 2216 36-37 -0.31770206050894245 2216 38-39 -0.3355539121710436 2216 40-41 -0.27688330746701695 2216 42-43 -0.26138672515762806 2216 44-45 -0.2558922871112159 2216 46-47 -0.24116685237255808 2216 48-49 -0.27242817762446236 2216 50-51 -0.27059376551971326 2216 52-53 -0.2650152536014829 2216 54-55 -0.3008295268833123 2216 56-57 -0.3062880195101201 2216 58-59 -0.20083336830953158 2216 60-61 -0.24569227234671587 2216 62-63 -0.29391815379563724 2216 64-65 -0.3214484173523289 2216 66-67 -0.3911683373741539 2216 68-69 -0.29222475236801415 2216 70-71 -0.3119129211497125 2216 72-73 -0.2381503411866177 2216 74-75 -0.335353853929675 2216 76-77 -0.37646272933006486 2216 78-79 -0.46204022835352987 2216 80-81 -0.33561292273827803 2216 82-83 -0.2712113651250192 2216 84-85 -0.3531600467758764 2216 86-87 -0.34652211552430856 2216 88-89 -0.45042861316461824 2216 90-91 -0.46373110502899095 2216 92-93 -0.44537270698878473 2216 94-95 -0.36465180068386616 2216 96-97 -0.5130013357955647 2216 98-99 -0.5704692573211929 2216 100 -0.5927234589712356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 5.0 4 14.0 5 17.0 6 46.0 7 144.0 8 266.0 9 528.0 10 980.0 11 1651.0 12 2505.0 13 3958.0 14 4995.0 15 6420.0 16 7916.0 17 11503.0 18 13551.0 19 7117.0 20 4769.0 21 4765.0 22 4825.0 23 5129.0 24 5920.0 25 7127.0 26 9085.0 27 12031.0 28 15577.0 29 19797.0 30 25636.0 31 32270.0 32 42066.0 33 55126.0 34 75610.0 35 114122.0 36 202437.0 37 465580.0 38 888442.0 39 456919.0 40 1955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54395332046597 20.192667832273713 13.802561891662297 23.46081695559802 2 15.374824427688164 20.396412136388644 40.65528098357226 23.573482452350934 3 18.061959126190082 25.648411312626656 30.364448579549077 25.92518098163419 4 11.989762388967087 17.103071522283052 39.4693281906305 31.43783789811936 5 12.154957103501618 37.99011554434006 36.2218820930767 13.633045259081628 6 30.171732945889723 38.97695451321223 17.728014427176493 13.12329811372155 7 26.591432281087453 32.85564078667527 22.83943094287922 17.71349598935806 8 24.715371190378896 36.66739153119267 20.469218349109095 18.14801892931934 9 24.694203487306936 16.605371950199537 21.690085310774965 37.01033925171856 10-11 23.535723310994545 26.977847140229354 29.194547006746248 20.291882542029853 12-13 24.53965238447997 24.241940879871915 29.623867899729966 21.594538835918144 14-15 21.54847422956743 26.231777071455127 27.029884935699126 25.189863763278314 16-17 20.627740270043738 29.818897485697377 27.500766185329027 22.052596058929858 18-19 20.453046476783115 28.20497195716747 30.40719658499555 20.934784981053863 20-21 21.611011862859915 27.487240719613652 30.113654127666283 20.788093289860143 22-23 21.194332124895578 27.35885822047998 30.208049768651048 21.238759885973394 24-25 21.02770749756301 27.49835957515237 30.138651993950095 21.335280933334527 26-27 20.83074951300712 27.414044910481326 30.274070153260872 21.48113542325068 28-29 20.869710155692566 27.46822892261811 30.29406604672151 21.367994874967813 30-31 21.164804125509747 27.933198636141142 29.872567375479775 21.029429862869335 32-33 20.81734254148047 27.615440374061063 30.36631459045252 21.20090249400594 34-35 20.91623453692419 27.402342660963352 30.356178459626737 21.32524434248572 36-37 21.34111565146048 27.824415927864205 29.717960666236525 21.11650775443879 38-39 21.2012012012012 27.947383723245796 29.776686500824432 21.074728574728574 40-41 20.9530802125364 27.77046276172258 29.931114055703784 21.345342970037244 42-43 21.023888610095508 27.651703427565494 29.793394189945914 21.53101377239308 44-45 21.21406552441035 27.846639743191464 29.603038051313913 21.33625668108427 46-47 21.275838172389896 28.18232688922344 29.212826885240677 21.329008053145984 48-49 21.31980787153201 28.029249408559753 29.03868059040473 21.61226212950351 50-51 21.360949809225673 28.046215977250462 28.85447781999506 21.738356393528807 52-53 21.320066751101233 28.05394253670116 28.946678774264985 21.679311937932628 54-55 21.481985964744585 28.432882484606626 28.706896551724135 21.378234998924654 56-57 21.2301558853283 28.166336097370582 28.778646020025327 21.82486199727579 58-59 21.061585458137184 27.76198612405509 29.248631921045714 21.927796496762014 60-61 21.389944320978806 28.171553516381103 28.797365004261554 21.641137158378537 62-63 21.255187548290998 28.07797850901299 28.892353892353896 21.774480050342117 64-65 21.19082610461921 27.917572745158953 29.073828470380192 21.817772679841646 66-67 21.098589524256923 28.009719535792993 28.8424755343823 22.049215405567786 68-69 21.545805771715024 28.172283604497107 28.72375825603933 21.55815236774854 70-71 21.59564269666591 27.97712618870576 28.716904723120223 21.710326391508108 72-73 21.344537681251197 28.146164725262075 28.802068335689146 21.707229257797582 74-75 21.333869679765336 28.211218269765 28.6872390040684 21.767673046401267 76-77 21.428019531467807 28.1821675787193 28.55196708644985 21.837845803363045 78-79 21.54586947690396 28.279127934300348 28.64739407842856 21.52760851036713 80-81 21.4612357715806 28.058562541321162 28.720159151193634 21.760042535904606 82-83 21.277202057577686 28.18403893713459 28.798918598790475 21.739840406497247 84-85 21.218924494786563 28.170776877673433 28.66390262941987 21.946395998120135 86-87 21.412480997160678 28.264336315506277 28.49874283547653 21.824439851856514 88-89 21.15183788240261 28.188842466653657 28.61297530239727 22.046344348546462 90-91 21.11713669399421 28.063216312407 28.71920872272158 22.10043827087721 92-93 21.294803857726905 28.25346850910365 28.47104812998222 21.980679503187226 94-95 21.007202668625666 27.993924658839564 28.717218438465263 22.281654234069503 96-97 21.023066826747584 28.05037092559842 28.651689980159816 22.27487226749418 98-99 21.014064750264456 28.167217350853267 28.541298924333763 22.277418974548514 100 21.10919938506145 27.97906659975625 28.433579468062227 22.478154547120063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 16.5 2 87.5 3 171.0 4 119.0 5 68.0 6 212.5 7 545.5 8 923.0 9 1082.5 10 914.0 11 761.5 12 823.5 13 990.5 14 1189.5 15 1364.0 16 1578.0 17 1876.5 18 2190.5 19 2571.5 20 3188.5 21 3988.5 22 5198.0 23 7179.0 24 9718.0 25 12692.0 26 16671.5 27 22180.5 28 29230.5 29 36379.0 30 43652.5 31 51507.0 32 59116.5 33 66842.0 34 73963.5 35 80065.5 36 84867.0 37 89290.0 38 92922.5 39 93708.0 40 94455.5 41 95177.5 42 94098.5 43 92323.0 44 90077.0 45 87844.5 46 86450.0 47 84487.5 48 81647.0 49 78495.0 50 75509.5 51 72434.5 52 67046.5 53 62232.0 54 59230.0 55 55613.5 56 50588.5 57 45997.5 58 41502.5 59 36818.0 60 32368.0 61 27066.0 62 22712.0 63 19085.5 64 15102.0 65 11475.0 66 8739.0 67 6382.0 68 4648.0 69 3531.0 70 2532.0 71 1753.0 72 1234.5 73 843.0 74 562.5 75 351.5 76 204.0 77 151.0 78 93.5 79 50.0 80 27.5 81 14.0 82 7.5 83 3.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04428832015038912 2 0.24509921061645198 3 0.002708278570347536 4 0.003106554830692762 5 3.584486343107033E-4 6 1.1948287810356776E-4 7 0.0 8 2.389657562071355E-4 9 0.0 10-11 6.173282035351001E-4 12-13 0.0 14-15 2.588795692243968E-4 16-17 1.7922431715535164E-4 18-19 7.168972686214066E-4 20-21 1.9913813017261293E-5 22-23 9.956906508630647E-5 24-25 0.0014935359762945969 26-27 1.1948287810356776E-4 28-29 2.588795692243968E-4 30-31 1.1948287810356776E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.1948287810356776E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.9913813017261293E-5 68-69 2.588795692243968E-4 70-71 3.9827626034522585E-5 72-73 3.584486343107033E-4 74-75 1.9913813017261294E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3939669112082904E-4 84-85 0.0 86-87 4.381038863797484E-4 88-89 0.0019913813017261293 90-91 0.0017524155455189937 92-93 5.974143905178388E-4 94-95 5.775005775005775E-4 96-97 2.588795692243968E-4 98-99 1.5931050413809034E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2510820.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.18628410570776 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58158728423427 52.79604848756036 2 13.570367710144943 18.23485160785442 3 3.885576253966831 7.831722903402216 4 1.5271209186409376 4.104063196143178 5 0.7581630399305361 2.5469078699610033 6 0.42962507640198183 1.7318947465247974 7 0.2891445469526309 1.3598583375422935 8 0.1924656157480847 1.0344839632184641 9 0.13292032459839134 0.8037380422701376 >10 0.5985398028951422 6.952005546843335 >50 0.026157775971130313 1.2086529825356593 >100 0.0077980743994800214 0.8552889456774149 >500 2.9643117535120176E-4 0.10607319741224083 >1k 2.371449402530203E-4 0.4344101730543726 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4296 0.17109948144430903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3631 0.1446141101313515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9913813017261293E-5 0.0 12-13 0.0 0.0 0.0 3.9827626034522585E-5 0.0 14-15 0.0 0.0 0.0 3.9827626034522585E-5 0.0 16-17 0.0 0.0 0.0 3.9827626034522585E-5 0.0 18-19 0.0 0.0 0.0 3.9827626034522585E-5 0.0 20-21 0.0 0.0 0.0 1.3939669112082904E-4 0.0 22-23 0.0 0.0 0.0 1.7922431715535164E-4 0.0 24-25 0.0 0.0 0.0 3.38534821293442E-4 0.0 26-27 0.0 0.0 0.0 4.7793151241427097E-4 0.0 28-29 0.0 0.0 0.0 0.0010156044638803259 0.0 30-31 0.0 0.0 0.0 0.0028476752614683652 0.0 32-33 0.0 0.0 0.0 0.007348197003369417 0.0 34-35 0.0 0.0 0.0 0.013441823786651373 0.0 36-37 0.0 0.0 0.0 0.024414334759162347 0.0 38-39 0.0 0.0 0.0 0.04526409698823492 0.0 40-41 0.0 0.0 0.0 0.07320317665145251 0.0 42-43 0.0 0.0 0.0 0.10235699890872305 0.0 44-45 0.0 0.0 0.0 0.13358185771978875 0.0 46-47 0.0 0.0 0.0 0.17139818863956796 0.0 48-49 0.0 0.0 0.0 0.2148302148302148 0.0 50-51 0.0 0.0 0.0 0.26479397169052343 0.0 52-53 0.0 0.0 0.0 0.3186210082761807 0.0 54-55 0.0 0.0 0.0 0.374280115659426 0.0 56-57 0.0 0.0 0.0 0.4385220764531109 0.0 58-59 0.0 0.0 0.0 0.5147919803092217 0.0 60-61 0.0 0.0 0.0 0.5975139595829251 0.0 62-63 0.0 0.0 0.0 0.6856126683712891 0.0 64-65 0.0 0.0 0.0 0.7782915541536232 0.0 66-67 0.0 0.0 0.0 0.8851689886172645 0.0 68-69 0.0 0.0 0.0 1.0062250579491958 0.0 70-71 0.0 0.0 0.0 1.1447654551102828 0.0 72-73 0.0 0.0 0.0 1.292406464820258 0.0 74-75 0.0 0.0 0.0 1.4379166965373862 0.0 76-77 0.0 0.0 0.0 1.5890227097123648 0.0 78-79 0.0 3.9827626034522585E-5 0.0 1.7658573693056452 0.0 80-81 0.0 3.9827626034522585E-5 0.0 1.9662301558853283 0.0 82-83 0.0 3.9827626034522585E-5 0.0 2.183489855903649 0.0 84-85 0.0 3.9827626034522585E-5 0.0 2.397742570156363 0.0 86-87 0.0 3.9827626034522585E-5 0.0 2.6160377884515817 0.0 88 0.0 3.9827626034522585E-5 0.0 2.798129694681419 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3745 0.0 48.076134 1 GTATCAA 6845 0.0 42.346706 1 TCAACGC 8110 0.0 35.00152 4 ATCAACG 8210 0.0 34.579323 3 CAACGCA 8325 0.0 34.097576 5 TATCAAC 8690 0.0 32.96049 2 AACGCAG 8785 0.0 32.365658 6 CCGATCG 60 0.00416318 31.331385 9 ACGCAGA 10185 0.0 27.732195 7 CGCAGAG 10260 0.0 27.34625 8 GTACATG 6325 0.0 24.516209 1 GCAGAGT 11415 0.0 24.291086 9 TACATGG 6260 0.0 23.63008 2 CCGGTCG 100 0.0017598945 23.498539 9 CGCGCAC 260 2.7284841E-11 23.498537 94 CGACGCG 145 5.456968E-12 22.688694 90-91 GTGGTAT 2000 0.0 22.38781 1 ACACCGT 505 0.0 22.335243 6 TACACCG 365 0.0 21.889048 5 AGAGTAC 10070 0.0 21.398369 10-11 >>END_MODULE