FastQCFastQC Report
Wed 25 May 2016
SRR1033075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383369
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35600.029.7146761
TCAACGC34500.028.7494074
ATCAACG35150.028.0840323
CAACGCA35450.027.8469525
GTACATG62450.027.4788721
GGTATCA23250.027.299161
AACGCAG38000.025.8475116
TACATGG63250.025.7146782
ACCGCGT1701.2325836E-724.9082058
ACATGGG65200.023.7199523
TATCAAC43050.023.2579782
ACGCAGA44600.021.6015137
CGCAGAG44750.020.9222728
CATGGGG45600.020.4110744
GGACCGT2902.7885108E-919.4464046
TGGACCG3204.5656634E-1019.0971245
TATACTG15500.018.8033225
TACACCG2552.9871808E-718.4346315
GTACCGT2051.743753E-518.3396976
GTATTAG10900.018.1159551
Produced by FastQC (version 0.11.5)