FastQCFastQC Report
Wed 25 May 2016
SRR1033075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383369
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.19287823245162625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.15792770653641966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.047.6747631
GTATCAA66950.040.754571
ATCAACG81000.033.536233
TCAACGC80950.033.3827744
TATCAAC82650.033.1130262
CAACGCA82550.032.678815
AACGCAG86100.031.386016
ACGCAGA97800.027.3429157
GTACATG62450.027.0900841
CGCAGAG99300.026.9304458
TACATGG63800.025.6197242
GCAGAGT109250.024.2626469
TATAACG2351.9645086E-1024.053542
ACATGGG66800.023.2875023
AGAGTAC96850.021.15764610-11
GAGTACT75750.021.1261112-13
GTCTCGC6850.020.638541
CATGGGG47700.020.4935114
GAGTACA48800.020.1824911
CAGAGTA107900.019.97093410-11
Produced by FastQC (version 0.11.5)