##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2383369 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68757754254587 34.0 31.0 34.0 31.0 34.0 2 32.68750705409024 34.0 31.0 34.0 31.0 34.0 3 32.797727922113616 34.0 31.0 34.0 31.0 34.0 4 36.293829029411725 37.0 37.0 37.0 35.0 37.0 5 36.27522595116409 37.0 37.0 37.0 35.0 37.0 6 36.343285911665376 37.0 37.0 37.0 35.0 37.0 7 36.298219453219374 37.0 37.0 37.0 35.0 37.0 8 36.30145353069541 37.0 37.0 37.0 35.0 37.0 9 38.09912187328106 39.0 39.0 39.0 37.0 39.0 10-11 38.06588300007259 39.0 39.0 39.0 35.0 39.0 12-13 38.0368109176548 39.0 39.0 39.0 35.0 39.0 14-15 39.527784619167235 41.0 39.0 41.0 37.0 41.0 16-17 39.52877082818481 41.0 39.0 41.0 37.0 41.0 18-19 39.5494275120638 41.0 39.5 41.0 37.0 41.0 20-21 39.54391032190148 41.0 40.0 41.0 37.0 41.0 22-23 39.468748859282805 41.0 39.0 41.0 36.0 41.0 24-25 39.41044714435742 41.0 39.0 41.0 36.0 41.0 26-27 39.302809594317964 41.0 39.0 41.0 36.0 41.0 28-29 39.247861115924565 41.0 39.0 41.0 36.0 41.0 30-31 39.23819098091819 41.0 39.0 41.0 36.0 41.0 32-33 39.18943122109921 41.0 39.0 41.0 35.0 41.0 34-35 39.10495395383594 41.0 39.0 41.0 35.0 41.0 36-37 39.005828094600545 41.0 39.0 41.0 35.0 41.0 38-39 38.90903590673538 41.0 39.0 41.0 35.0 41.0 40-41 38.80500123984159 41.0 39.0 41.0 35.0 41.0 42-43 38.67975269461003 41.0 39.0 41.0 35.0 41.0 44-45 38.512049539957935 41.0 38.0 41.0 35.0 41.0 46-47 38.29865832777048 40.5 38.0 41.0 34.0 41.0 48-49 38.08435517118835 40.0 38.0 41.0 34.0 41.0 50-51 37.9430392440281 40.0 38.0 41.0 34.0 41.0 52-53 37.80531634002121 40.0 38.0 41.0 33.0 41.0 54-55 37.653778957433786 40.0 37.0 41.0 33.0 41.0 56-57 37.5022094774246 40.0 37.0 41.0 33.0 41.0 58-59 37.32374949074189 40.0 37.0 41.0 33.0 41.0 60-61 37.1300864448602 40.0 36.0 41.0 33.0 41.0 62-63 36.89754502974571 39.0 36.0 41.0 32.0 41.0 64-65 36.631203141435506 39.0 35.0 41.0 32.0 41.0 66-67 36.357663248955575 39.0 35.0 41.0 31.5 41.0 68-69 36.049384925288535 38.0 35.0 40.5 31.0 41.0 70-71 35.72408133192972 37.0 35.0 40.0 31.0 41.0 72-73 35.38145582996171 37.0 35.0 39.5 31.0 41.0 74-75 35.041047777326966 36.0 35.0 39.0 31.0 41.0 76-77 34.69022002048361 36.0 35.0 39.0 30.0 40.5 78-79 34.330389251517495 35.0 34.5 37.5 30.0 39.5 80-81 34.00069628328639 35.0 34.0 37.0 30.0 39.0 82-83 33.70070475868403 35.0 34.0 37.0 30.0 39.0 84-85 33.472418035142695 35.0 34.0 36.0 30.0 37.5 86-87 33.25551205037911 35.0 34.0 36.0 30.0 37.0 88-89 33.03945905984344 35.0 34.0 36.0 29.0 37.0 90-91 32.851377189180525 35.0 34.0 35.0 29.0 36.0 92-93 32.66965837014747 35.0 34.0 35.0 29.0 36.0 94-95 32.51019124608905 35.0 34.0 35.0 29.0 36.0 96-97 32.359910278265765 35.0 34.0 35.0 29.0 36.0 98-99 32.22888776349781 35.0 34.0 35.0 29.0 35.5 100 32.120834835059114 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16496173077967313 1101 2 0.130683173135985 1101 3 0.126867148136121 1101 4 0.10180083045506905 1101 5 0.10042987324597163 1101 6 0.1290482004653839 1101 7 0.18532161917829626 1101 8 0.1611202201112505 1101 9 0.23376476520936507 1101 10-11 0.23375706500109317 1101 12-13 0.19490432030567462 1101 14-15 0.3154472241575803 1101 16-17 0.32884223156778347 1101 18-19 0.30286020231103095 1101 20-21 0.2848699949170168 1101 22-23 0.3064543412180498 1101 24-25 0.3671348767780884 1101 26-27 0.3973072810893754 1101 28-29 0.4104482179065343 1101 30-31 0.38323511801750954 1101 32-33 0.37008104211636095 1101 34-35 0.3415863987241252 1101 36-37 0.30177102924348276 1101 38-39 0.303231257295117 1101 40-41 0.3156782955523312 1101 42-43 0.3616577061923607 1101 44-45 0.22883318986992407 1101 46-47 0.16837470362126084 1101 48-49 0.23244250083266138 1101 50-51 0.2056036363852911 1101 52-53 0.18038310146436487 1101 54-55 0.19493282367592002 1101 56-57 0.22001251439044012 1101 58-59 0.25704103465587025 1101 60-61 0.2839808485848536 1101 62-63 0.15186842412171586 1101 64-65 0.20338910062540094 1101 66-67 0.2829806421409202 1101 68-69 0.25620731872810865 1101 70-71 0.23437883940346893 1101 72-73 0.22769916358914344 1101 74-75 0.21387873432685467 1101 76-77 0.1642023282351346 1101 78-79 0.12220802099790262 1101 80-81 0.20181403201970483 1101 82-83 0.261319579826079 1101 84-85 0.31645209506383054 1101 86-87 0.32115290278036923 1101 88-89 0.29595835428705897 1101 90-91 0.2415295046083017 1101 92-93 0.25206831090139303 1101 94-95 0.2709788122561463 1101 96-97 0.32296579299454464 1101 98-99 0.28406033241310524 1101 100 0.24247458184150616 1102 1 0.10075830276849018 1102 2 0.10903770053459283 1102 3 0.16386951556865625 1102 4 0.06356786497834577 1102 5 0.056231152865628076 1102 6 0.10674949545292378 1102 7 0.12013396562101519 1102 8 0.13552675710408835 1102 9 0.18537926860294363 1102 10-11 0.1583964546431389 1102 12-13 0.18782629035191434 1102 14-15 0.24232906067829418 1102 16-17 0.20640079002119194 1102 18-19 0.17000303027436559 1102 20-21 0.2485265499038931 1102 22-23 0.2601090773475576 1102 24-25 0.2975491385651523 1102 26-27 0.28284303671276234 1102 28-29 0.2649040780315346 1102 30-31 0.2838448242963594 1102 32-33 0.2837346305176922 1102 34-35 0.3129703254718308 1102 36-37 0.36939716748832296 1102 38-39 0.3580963116954905 1102 40-41 0.38901229028833484 1102 42-43 0.329511590668659 1102 44-45 0.3662092984488936 1102 46-47 0.33770789300224635 1102 48-49 0.3020278986177374 1102 50-51 0.25907794575444854 1102 52-53 0.2288149448908996 1102 54-55 0.2731570644731889 1102 56-57 0.3697966345011636 1102 58-59 0.2962976180441643 1102 60-61 0.31664811051393116 1102 62-63 0.2464094203886802 1102 64-65 0.30084941160138357 1102 66-67 0.32210815474160626 1102 68-69 0.23226546575014595 1102 70-71 0.19251501637926793 1102 72-73 0.20215136654639565 1102 74-75 0.2274562127874944 1102 76-77 0.20935035599070062 1102 78-79 0.18058823610940067 1102 80-81 0.23314929703792586 1102 82-83 0.22851507684101335 1102 84-85 0.27058615360574834 1102 86-87 0.34382306320942746 1102 88-89 0.2978674736682976 1102 90-91 0.2868062140910652 1102 92-93 0.3614545875739168 1102 94-95 0.3453738719671975 1102 96-97 0.2724391511110227 1102 98-99 0.31589991697441633 1102 100 0.320131815738776 1103 1 0.1357100300993963 1103 2 0.10812081485934044 1103 3 0.1678230176634159 1103 4 0.10245493825203766 1103 5 0.11560517413555971 1103 6 0.1451043665215508 1103 7 0.12813515484896953 1103 8 0.148718336280794 1103 9 0.15862958293132579 1103 10-11 0.17968851450397239 1103 12-13 0.18316526570946934 1103 14-15 0.30478962778570207 1103 16-17 0.29528650158348313 1103 18-19 0.2677171707394379 1103 20-21 0.2830646573973965 1103 22-23 0.23678750012263805 1103 24-25 0.25212528319706706 1103 26-27 0.1956458472850855 1103 28-29 0.22162037907869347 1103 30-31 0.23369299347537975 1103 32-33 0.22496940771900853 1103 34-35 0.243413539836979 1103 36-37 0.26924442885974287 1103 38-39 0.27865860772246975 1103 40-41 0.2641564047447247 1103 42-43 0.3127086393861589 1103 44-45 0.30852096241483906 1103 46-47 0.28491490944718123 1103 48-49 0.25795270491429534 1103 50-51 0.2324264167794965 1103 52-53 0.19920396314236655 1103 54-55 0.2819945714519534 1103 56-57 0.30556981423345064 1103 58-59 0.2976218823795804 1103 60-61 0.3075592543061205 1103 62-63 0.25535699903885956 1103 64-65 0.277250953058676 1103 66-67 0.2583556639445064 1103 68-69 0.25340773735709377 1103 70-71 0.3521705714809187 1103 72-73 0.25837245854815905 1103 74-75 0.23382902213428025 1103 76-77 0.26681003798569947 1103 78-79 0.3066010089623177 1103 80-81 0.3343755181526191 1103 82-83 0.359714835364187 1103 84-85 0.32144947863264406 1103 86-87 0.2770180693598263 1103 88-89 0.23539669372540573 1103 90-91 0.2501506453722939 1103 92-93 0.31525512408821044 1103 94-95 0.22268821396554728 1103 96-97 0.2955848406135928 1103 98-99 0.3180695771366402 1103 100 0.3900500222740888 1104 1 0.06587345641666786 1104 2 0.038472087518307774 1104 3 0.05263094477903962 1104 4 0.05713231216017789 1104 5 0.06178719724066184 1104 6 0.052082858115426234 1104 7 0.09266976553742978 1104 8 0.07931850534250628 1104 9 0.047410460173736624 1104 10-11 0.10389955986886434 1104 12-13 0.12961027369293987 1104 14-15 0.16761483676474853 1104 16-17 0.1840438980331811 1104 18-19 0.15708976395817587 1104 20-21 0.12955662696628423 1104 22-23 0.08665600768345172 1104 24-25 0.17778274539298167 1104 26-27 0.16348890166659658 1104 28-29 0.13005937597922923 1104 30-31 0.07902970804286724 1104 32-33 0.14570294768331848 1104 34-35 0.06824682620872835 1104 36-37 0.07872127679812735 1104 38-39 0.056147571749896485 1104 40-41 0.05414889089105657 1104 42-43 0.07332900192959357 1104 44-45 0.06718302684613064 1104 46-47 0.05069869830789031 1104 48-49 -0.052085333969898784 1104 50-51 0.012411389072163104 1104 52-53 0.023213073689944963 1104 54-55 -0.030142650300653884 1104 56-57 -0.002650811294863331 1104 58-59 0.00987885617501405 1104 60-61 0.07699195835420625 1104 62-63 0.0037039512319694268 1104 64-65 -0.04662901312897816 1104 66-67 -0.020530407249253813 1104 68-69 0.035494240597444104 1104 70-71 0.03753968954234921 1104 72-73 0.010974130380596137 1104 74-75 -0.027293829373185474 1104 76-77 0.007107774437280057 1104 78-79 -0.029395046264504288 1104 80-81 -0.007544741075335537 1104 82-83 -0.03497615740372595 1104 84-85 -0.010679290034588007 1104 86-87 0.012180556445386514 1104 88-89 0.007560213965845719 1104 90-91 0.034582879804531785 1104 92-93 0.06591023820486441 1104 94-95 0.07059842341422495 1104 96-97 0.036871118438327244 1104 98-99 0.01910742817449318 1104 100 0.0010484255801799236 1105 1 0.052586829612856434 1105 2 0.04828720438264611 1105 3 0.05507867019238688 1105 4 0.030013178511239857 1105 5 0.05760315471874833 1105 6 0.05914254342146563 1105 7 0.05916188089177865 1105 8 0.10787654141970648 1105 9 0.08516508744110496 1105 10-11 0.11477365979811793 1105 12-13 0.08980721254435053 1105 14-15 0.16988933693685482 1105 16-17 0.14603583263827602 1105 18-19 0.11598327583833878 1105 20-21 0.08973306937127035 1105 22-23 0.10741562155461537 1105 24-25 0.105655015344162 1105 26-27 0.16379894284975194 1105 28-29 0.153025255017333 1105 30-31 0.13747316293078882 1105 32-33 0.12440701013088784 1105 34-35 0.108375971117745 1105 36-37 0.12727515304802495 1105 38-39 0.10970452679089249 1105 40-41 0.08914942708501172 1105 42-43 0.05350513299615045 1105 44-45 0.1050710539809998 1105 46-47 0.18834967582407103 1105 48-49 0.2215060326155367 1105 50-51 0.1044544869586943 1105 52-53 0.07079842798442826 1105 54-55 0.02421470394539682 1105 56-57 -0.023954359342162945 1105 58-59 6.865026467721691E-4 1105 60-61 2.0132998677979685E-4 1105 62-63 0.036701413823791995 1105 64-65 0.024270011170990813 1105 66-67 0.011348874735148229 1105 68-69 -0.002826114535920965 1105 70-71 0.0575252382503777 1105 72-73 0.09388805049461979 1105 74-75 0.006829467562376124 1105 76-77 -0.038664855109772134 1105 78-79 -0.04515080299849217 1105 80-81 -0.02051008391947562 1105 82-83 -0.036085862687315284 1105 84-85 0.0581498093844246 1105 86-87 0.042734444802889016 1105 88-89 0.0155044030935656 1105 90-91 0.0949054350627776 1105 92-93 0.10905858750582098 1105 94-95 0.06357142864297316 1105 96-97 0.13699677808661903 1105 98-99 0.1789139399906503 1105 100 0.19641095323309088 1106 1 -0.0041873307877224875 1106 2 -0.047111987984379766 1106 3 0.04518636345679994 1106 4 0.018734051630126203 1106 5 -6.35808999618348E-4 1106 6 0.06093133439630094 1106 7 0.03175836137319976 1106 8 0.081571603605056 1106 9 0.05432357418845157 1106 10-11 0.06610883479801544 1106 12-13 0.057673531429344393 1106 14-15 0.0737354620045707 1106 16-17 0.09253206034780703 1106 18-19 0.09169505103130149 1106 20-21 0.08794095288802595 1106 22-23 0.04582895165555101 1106 24-25 0.06707379695103555 1106 26-27 0.07281802095896239 1106 28-29 0.06246255737821116 1106 30-31 0.033337319570222235 1106 32-33 0.020483999308687828 1106 34-35 -0.008050203094335018 1106 36-37 -0.0012234896457172795 1106 38-39 -0.018873969673592228 1106 40-41 -0.003384548803055054 1106 42-43 0.023829335148491282 1106 44-45 -0.05839875988031196 1106 46-47 -0.13888450880616432 1106 48-49 -0.04562341180175622 1106 50-51 -0.11645176230346266 1106 52-53 -0.01607511689780239 1106 54-55 -0.06367170766142038 1106 56-57 -0.20043726173949494 1106 58-59 -0.18179403730937338 1106 60-61 -0.2425528444407945 1106 62-63 -0.24921810920518794 1106 64-65 -0.20863616892059156 1106 66-67 -0.2602635628658376 1106 68-69 -0.18921562335499686 1106 70-71 -0.1494390248385713 1106 72-73 -0.09930432617641571 1106 74-75 -0.08375995885060661 1106 76-77 -0.1567112170202023 1106 78-79 -0.16855651269690952 1106 80-81 -0.15103653850516707 1106 82-83 -0.13927148358799712 1106 84-85 -0.1603667711016925 1106 86-87 -0.14668289785650046 1106 88-89 -0.16798168746867503 1106 90-91 -0.21817156143114147 1106 92-93 -0.21152512069544827 1106 94-95 -0.12206335604199126 1106 96-97 -0.10954273942866877 1106 98-99 -0.13414364384791355 1106 100 -0.1686872207634984 1107 1 0.020505728501291287 1107 2 0.01659299656161295 1107 3 0.028552228226701004 1107 4 0.05435471620270249 1107 5 0.037619461470647764 1107 6 0.03715381166833964 1107 7 0.06260156110741377 1107 8 0.018521652433669544 1107 9 0.026287705290769736 1107 10-11 0.040598714186295126 1107 12-13 0.038037317207631816 1107 14-15 0.028354579918122624 1107 16-17 0.0730902796638091 1107 18-19 0.08396914946375489 1107 20-21 0.08201529193984669 1107 22-23 0.1137469774692832 1107 24-25 0.07923863506152173 1107 26-27 0.04937084647468737 1107 28-29 0.07351286343799757 1107 30-31 0.0913474150125495 1107 32-33 0.07200338099745096 1107 34-35 0.10646454365126345 1107 36-37 0.06605240210576824 1107 38-39 0.1253557588997083 1107 40-41 0.07925001934054876 1107 42-43 0.10993177330729509 1107 44-45 0.0809438596994525 1107 46-47 0.13092710460207257 1107 48-49 0.053068125997640436 1107 50-51 -0.011631163422990198 1107 52-53 0.06571942285472687 1107 54-55 -0.010205945582633547 1107 56-57 -0.018292993312989836 1107 58-59 0.03251199226968993 1107 60-61 0.0504542495517768 1107 62-63 0.08818417063815787 1107 64-65 0.09323212343697662 1107 66-67 0.07249402067136401 1107 68-69 0.07778818729065051 1107 70-71 0.12864529513485223 1107 72-73 0.06804804905892325 1107 74-75 -0.006662971123205352 1107 76-77 0.03464253120536398 1107 78-79 0.0418836558876734 1107 80-81 0.06569582026938292 1107 82-83 0.05614712112169684 1107 84-85 0.021690977546711565 1107 86-87 -0.010096011863772958 1107 88-89 0.012297214541852952 1107 90-91 0.018332454345035387 1107 92-93 0.07052778284538164 1107 94-95 0.01909098769132811 1107 96-97 0.018015571755604753 1107 98-99 0.08833394281029427 1107 100 0.05757301059353637 1108 1 -0.015610999939234205 1108 2 0.005327920320198132 1108 3 -0.0029342529574236664 1108 4 0.015194745057328873 1108 5 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52-53 -0.2730360855216176 2214 54-55 -0.25246823202670754 2214 56-57 -0.25493444958732425 2214 58-59 -0.23854664748036924 2214 60-61 -0.28115821463845947 2214 62-63 -0.25717873087250354 2214 64-65 -0.2934031585110759 2214 66-67 -0.3028142304085151 2214 68-69 -0.33809010630368164 2214 70-71 -0.2812544029678463 2214 72-73 -0.2828196066165134 2214 74-75 -0.412551483925391 2214 76-77 -0.27469806138108765 2214 78-79 -0.23793571482818265 2214 80-81 -0.24576325079293326 2214 82-83 -0.2250712429330619 2214 84-85 -0.18314512167212627 2214 86-87 -0.19235863460592384 2214 88-89 -0.19329269747299804 2214 90-91 -0.10322191933768465 2214 92-93 -0.18352673572465505 2214 94-95 -0.19579058753416234 2214 96-97 -0.11543843675791976 2214 98-99 -0.10039833042644375 2214 100 -0.09746332554928472 2215 1 -0.15738612205405644 2215 2 -0.14646950650546842 2215 3 -0.21926145260034957 2215 4 -0.1606626434884646 2215 5 -0.1627873360037171 2215 6 -0.1550746083339476 2215 7 -0.20250791331719853 2215 8 -0.17344402077065268 2215 9 -0.23197655051215094 2215 10-11 -0.19273731136455297 2215 12-13 -0.2042065404469966 2215 14-15 -0.3057353858123335 2215 16-17 -0.28460472548889015 2215 18-19 -0.2944018306124718 2215 20-21 -0.3665019266654923 2215 22-23 -0.3470306508165919 2215 24-25 -0.41159001926592254 2215 26-27 -0.46719447013009585 2215 28-29 -0.3873946047052357 2215 30-31 -0.4239067235051124 2215 32-33 -0.35867312555384245 2215 34-35 -0.3804640587402517 2215 36-37 -0.3560457282002858 2215 38-39 -0.332011272818022 2215 40-41 -0.3786087680574184 2215 42-43 -0.3854699021380412 2215 44-45 -0.428517325339719 2215 46-47 -0.3400242943968266 2215 48-49 -0.28192816283163324 2215 50-51 -0.27263151307048616 2215 52-53 -0.30143446702471266 2215 54-55 -0.3880854255688959 2215 56-57 -0.31765411987310443 2215 58-59 -0.2899233127210721 2215 60-61 -0.30476383029053267 2215 62-63 -0.2700363281104714 2215 64-65 -0.3408606026683998 2215 66-67 -0.31221329779820195 2215 68-69 -0.26872474353231723 2215 70-71 -0.29860502384180876 2215 72-73 -0.2801714579994581 2215 74-75 -0.2650872264353836 2215 76-77 -0.3257423059529714 2215 78-79 -0.2939013845160474 2215 80-81 -0.3126820607441516 2215 82-83 -0.3308876023500744 2215 84-85 -0.25206153746155024 2215 86-87 -0.2871096031319951 2215 88-89 -0.37673060185845486 2215 90-91 -0.27934254320130236 2215 92-93 -0.2923904650730975 2215 94-95 -0.26498328479652145 2215 96-97 -0.3483835600553675 2215 98-99 -0.34312952618353876 2215 100 -0.288688419403357 2216 1 -0.21076233811210443 2216 2 -0.18001689284358235 2216 3 -0.21318245029856797 2216 4 -0.21336764496184912 2216 5 -0.17584250861646922 2216 6 -0.20211918287591146 2216 7 -0.19834729989435118 2216 8 -0.16959668203421785 2216 9 -0.23138209621079397 2216 10-11 -0.2501052424637038 2216 12-13 -0.29248903168394946 2216 14-15 -0.3200020057015891 2216 16-17 -0.27553289640982825 2216 18-19 -0.3143513645899745 2216 20-21 -0.3788002892501581 2216 22-23 -0.37339458832032335 2216 24-25 -0.3731627138300624 2216 26-27 -0.3807397236657053 2216 28-29 -0.3496250421667213 2216 30-31 -0.37851038285842975 2216 32-33 -0.3656275557040374 2216 34-35 -0.348138989759029 2216 36-37 -0.36241799084616133 2216 38-39 -0.3981702397585565 2216 40-41 -0.39051923362656993 2216 42-43 -0.3520933460680027 2216 44-45 -0.3207060907252597 2216 46-47 -0.27167716997098523 2216 48-49 -0.37284041500925724 2216 50-51 -0.43771081314033466 2216 52-53 -0.511790356547607 2216 54-55 -0.4362527609258109 2216 56-57 -0.44338702602773594 2216 58-59 -0.49599971341592664 2216 60-61 -0.44239960326143546 2216 62-63 -0.4343759733027994 2216 64-65 -0.5283629982074487 2216 66-67 -0.47999571897208426 2216 68-69 -0.48754623620557425 2216 70-71 -0.49444513165652637 2216 72-73 -0.43433073198112737 2216 74-75 -0.5266463720367653 2216 76-77 -0.516295021206318 2216 78-79 -0.6386888461101421 2216 80-81 -0.5906165187959402 2216 82-83 -0.49810753550166 2216 84-85 -0.5728887917925789 2216 86-87 -0.6276788038153143 2216 88-89 -0.8217833243241799 2216 90-91 -0.756693597341517 2216 92-93 -0.7148053951027471 2216 94-95 -0.6263615285624553 2216 96-97 -0.7513931813644348 2216 98-99 -0.8073418471878284 2216 100 -0.8564037654840497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 1.0 4 8.0 5 21.0 6 52.0 7 135.0 8 302.0 9 617.0 10 1163.0 11 1812.0 12 2692.0 13 4490.0 14 5279.0 15 6523.0 16 8130.0 17 11725.0 18 13838.0 19 7242.0 20 4910.0 21 4691.0 22 4763.0 23 5056.0 24 5765.0 25 7163.0 26 9042.0 27 11560.0 28 14824.0 29 19280.0 30 24945.0 31 32035.0 32 40235.0 33 52692.0 34 72949.0 35 110961.0 36 201052.0 37 457754.0 38 824927.0 39 412945.0 40 1776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04368284675807 19.821343045949536 13.411486481097715 23.723487626194682 2 15.659005692631546 20.3078268952131 40.25331894929205 23.779848462863306 3 18.0400670329366 25.57762200909157 30.32669745303373 26.055613504938098 4 12.068798406922134 16.9712639265291 39.3253335380905 31.634604128458264 5 12.11761522049707 37.86465774230972 36.07764652078049 13.94008051641272 6 30.343950241842638 38.81838641787588 17.606513136032707 13.231150204248776 7 26.62411905164496 32.802641974448775 22.850679017810503 17.72255995609576 8 24.841211700111018 36.143565266207986 20.54232634404677 18.472896689634222 9 24.94288547010555 16.21675032275741 21.509971808813493 37.330392398323546 10-11 23.64916149642961 26.556703937687786 29.28047167163236 20.513662894250242 12-13 24.79337022508894 24.063919602881466 29.41181159946278 21.73089857256682 14-15 21.790273947112095 25.93089634115513 27.000037552003757 25.27879215972902 16-17 20.782582280690416 29.434841312664528 27.38035198958112 22.402224417063934 18-19 20.46801623340183 27.91415052085136 30.406328047722592 21.211505198024216 20-21 21.843092697773614 27.246116736434857 29.967055038477046 20.943735527314487 22-23 21.341016592727044 27.13121603146142 30.084794031627993 21.442973344183546 24-25 21.261049883996805 27.182006592010914 30.0340971145739 21.52284640941838 26-27 21.040155846945748 27.01104405369889 30.268267876832915 21.68053222252245 28-29 21.04899673276794 27.221808008263952 30.185099793862708 21.5440954651054 30-31 21.347290892552053 27.684583329137592 29.742196756858363 21.225929021451996 32-33 20.917491164817534 27.31691987266764 30.31484004365249 21.450748918862335 34-35 21.066041389310676 27.066308238464128 30.28297338767098 21.584676984554214 36-37 21.562859129241 27.554965261359026 29.56558552200687 21.3165900873931 38-39 21.41365856482987 27.71931245224722 29.645451459677457 21.221577523245454 40-41 21.16575440663448 27.454359201574746 29.790172850042158 21.589713541748615 42-43 21.15702604170819 27.43761037422237 29.661038639002186 21.744324945067255 44-45 21.40738593142732 27.588887830629666 29.462265389874588 21.54146084806843 46-47 21.51301372133312 27.963252857614577 28.992929756156094 21.530803664896204 48-49 21.49362939603561 27.792213459183195 28.916588241266876 21.79756890351431 50-51 21.53810425494332 27.734752780622724 28.806785688661723 21.920357275772236 52-53 21.555076028932156 27.788059675190873 28.707283681209244 21.949580614667724 54-55 21.651032634896232 28.2318642224515 28.48677649159656 21.63032665105571 56-57 21.441539266475313 27.900002055913287 28.620305961854836 22.038152715756564 58-59 21.278891350856707 27.46488269336389 29.10485115817148 22.15137479760792 60-61 21.660242287283253 27.906022944831456 28.621648599104883 21.81208616878041 62-63 21.425637406545107 27.915987830671625 28.635263779968607 22.02311098281466 64-65 21.435056846002446 27.59897858871203 28.865987599905846 22.09997696537968 66-67 21.321310296475286 27.717655134391695 28.640487478019562 22.32054709111346 68-69 21.82381709214371 27.862802324781278 28.51213662705319 21.801243956021825 70-71 21.81993678275097 27.659004472031917 28.525949722000604 21.99510902321651 72-73 21.61094286138656 27.764812898753945 28.694256264889685 21.929987974969812 74-75 21.612304790140158 27.819202643993396 28.53497650065695 22.033516065209497 76-77 21.641718088974052 27.88777146971367 28.343240178084052 22.12727026322823 78-79 21.801387028194124 28.00258793329946 28.38091793591341 21.81510710259301 80-81 21.68845864824121 27.78310870033134 28.505258732491694 22.02317391893576 82-83 21.533527987926323 27.797607334497794 28.60025333907871 22.068611338497178 84-85 21.510139638469745 27.802681833992132 28.455161580099432 22.232016947438687 86-87 21.69332826206034 27.86199480187525 28.35680055820366 22.087876377860745 88-89 21.414824064307094 27.788596047352556 28.409202480243895 22.387377408096455 90-91 21.257051627669863 27.695038213580663 28.526820835684752 22.52108932306472 92-93 21.476550053642914 27.848056918131725 28.367577106706392 22.30781592151897 94-95 21.25597267046971 27.60080743288036 28.507700905781597 22.635518990868338 96-97 21.23327415924473 27.577104284995613 28.556518667110296 22.633102888649358 98-99 21.224310491968083 27.650579055000364 28.457861696441082 22.667248756590467 100 21.35191823003488 27.591237445817246 28.24380110675267 22.813043217395208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 36.0 1 32.5 2 100.5 3 207.5 4 159.0 5 93.0 6 245.0 7 600.5 8 1062.5 9 1255.5 10 990.0 11 777.5 12 856.0 13 977.0 14 1148.5 15 1377.0 16 1583.0 17 1817.5 18 2144.0 19 2581.0 20 3157.5 21 3967.5 22 5114.0 23 6893.5 24 9233.0 25 12186.0 26 16098.5 27 21035.0 28 27406.5 29 34441.0 30 41366.5 31 48370.5 32 55189.0 33 61925.5 34 68379.5 35 73277.0 36 76562.5 37 80211.5 38 83406.5 39 84475.0 40 85349.5 41 85396.5 42 84482.0 43 83458.0 44 81567.5 45 79922.0 46 78643.5 47 76948.0 48 75591.0 49 74346.0 50 72292.0 51 69482.5 52 64917.5 53 61329.5 54 59348.0 55 56279.0 56 51999.5 57 47911.0 58 43969.5 59 40073.5 60 35774.5 61 30369.5 62 25511.0 63 20930.0 64 16504.0 65 13003.5 66 10065.5 67 7405.0 68 5605.5 69 4246.5 70 2986.5 71 2148.5 72 1582.0 73 1081.0 74 749.5 75 551.5 76 351.0 77 211.5 78 127.0 79 65.5 80 34.0 81 16.5 82 8.5 83 4.5 84 2.0 85 0.5 86 1.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04355179579830064 2 0.24058381224225037 3 0.0026433170860240274 4 0.00243353001570466 5 1.678296562554938E-4 6 4.195741406387345E-5 7 0.0 8 2.937018984471142E-4 9 0.0 10-11 6.713186250219752E-4 12-13 0.0 14-15 3.1468060547905086E-4 16-17 1.2587224219162035E-4 18-19 4.8251026173454464E-4 20-21 0.0 22-23 2.3076577735130398E-4 24-25 0.0012377437148842667 26-27 2.0978707031936723E-4 28-29 2.517444843832407E-4 30-31 4.195741406387345E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.0489353515968362E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0978707031936723E-4 70-71 2.0978707031936725E-5 72-73 2.937018984471142E-4 74-75 1.8880836328743052E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.39148281277469E-5 84-85 0.0 86-87 3.985954336067978E-4 88-89 0.0023076577735130395 90-91 0.002265700359449166 92-93 6.713186250219752E-4 94-95 5.454463828303549E-4 96-97 2.517444843832407E-4 98-99 1.2587224219162035E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2383369.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.87522206010493 #Duplication Level Percentage of deduplicated Percentage of total 1 81.35247297690013 57.65874587376483 2 12.279145334866485 17.40574304633927 3 3.1708511298534843 6.742043338437011 4 1.218475745818427 3.454389562389321 5 0.6164307470925371 2.1844833042429976 6 0.3350064100718432 1.4246192223240275 7 0.2240270604131764 1.1114577357979496 8 0.15126750957118337 0.8576894665069338 9 0.11139447092391506 0.7105597076700345 >10 0.5147590822007388 6.258288434170703 >50 0.02035221952676784 0.969659916380917 >100 0.005343833049619202 0.6516249996255989 >500 2.367398558347823E-4 0.0932072886318632 >1k 2.3673985582655212E-4 0.4774881037185293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4597 0.19287823245162625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3764 0.15792770653641966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.39148281277469E-5 0.0 5 0.0 0.0 0.0 8.39148281277469E-5 0.0 6 0.0 0.0 0.0 8.39148281277469E-5 0.0 7 0.0 0.0 0.0 8.39148281277469E-5 0.0 8 0.0 0.0 0.0 8.39148281277469E-5 0.0 9 0.0 0.0 0.0 2.517444843832407E-4 0.0 10-11 0.0 0.0 0.0 2.517444843832407E-4 0.0 12-13 0.0 0.0 0.0 2.937018984471141E-4 0.0 14-15 0.0 0.0 0.0 2.937018984471141E-4 0.0 16-17 0.0 4.195741406387345E-5 0.0 2.937018984471141E-4 0.0 18-19 0.0 8.39148281277469E-5 0.0 2.937018984471141E-4 0.0 20-21 0.0 8.39148281277469E-5 0.0 3.566380195429243E-4 0.0 22-23 0.0 8.39148281277469E-5 0.0 4.195741406387345E-4 0.0 24-25 0.0 8.39148281277469E-5 0.0 4.6153155470260795E-4 0.0 26-27 0.0 8.39148281277469E-5 0.0 5.664250898622916E-4 0.0 28-29 0.0 8.39148281277469E-5 0.0 0.0010489353515968363 2.0978707031936725E-5 30-31 0.0 8.39148281277469E-5 0.0 0.0033775718321418127 4.195741406387345E-5 32-33 0.0 8.39148281277469E-5 0.0 0.00851735505496631 4.195741406387345E-5 34-35 0.0 8.39148281277469E-5 0.0 0.01615360441459128 4.195741406387345E-5 36-37 0.0 8.39148281277469E-5 0.0 0.029999551055669517 4.195741406387345E-5 38-39 0.0 8.39148281277469E-5 0.0 0.05492225500961034 4.195741406387345E-5 40-41 0.0 8.39148281277469E-5 0.0 0.08727142125285678 4.195741406387345E-5 42-43 0.0 8.39148281277469E-5 0.0 0.12243173423838272 4.195741406387345E-5 44-45 2.0978707031936725E-5 8.39148281277469E-5 0.0 0.16203953311467928 4.195741406387345E-5 46-47 4.195741406387345E-5 8.39148281277469E-5 0.0 0.20936749617872852 4.195741406387345E-5 48-49 4.195741406387345E-5 8.39148281277469E-5 0.0 0.26586315421573414 4.195741406387345E-5 50-51 4.195741406387345E-5 8.39148281277469E-5 0.0 0.32642868141693543 4.195741406387345E-5 52-53 4.195741406387345E-5 8.39148281277469E-5 0.0 0.38860956905959587 4.195741406387345E-5 54-55 4.195741406387345E-5 8.39148281277469E-5 0.0 0.4527834338702903 4.195741406387345E-5 56-57 4.195741406387345E-5 8.39148281277469E-5 0.0 0.5308661814431588 4.195741406387345E-5 58-59 4.195741406387345E-5 8.39148281277469E-5 0.0 0.6232354285047762 4.195741406387345E-5 60-61 4.195741406387345E-5 8.39148281277469E-5 0.0 0.7258632633050106 4.195741406387345E-5 62-63 4.195741406387345E-5 8.39148281277469E-5 0.0 0.8310085429490774 4.195741406387345E-5 64-65 4.195741406387345E-5 8.39148281277469E-5 0.0 0.9373286301869328 4.195741406387345E-5 66-67 4.195741406387345E-5 8.39148281277469E-5 0.0 1.061249852624583 4.195741406387345E-5 68-69 4.195741406387345E-5 8.39148281277469E-5 0.0 1.2022687212932617 4.195741406387345E-5 70-71 4.195741406387345E-5 8.39148281277469E-5 0.0 1.3602593639507772 4.195741406387345E-5 72-73 4.195741406387345E-5 8.39148281277469E-5 0.0 1.5343406749017883 4.195741406387345E-5 74-75 1.2587224219162035E-4 8.39148281277469E-5 0.0 1.7032822026299748 4.195741406387345E-5 76-77 1.2587224219162035E-4 8.39148281277469E-5 0.0 1.8846850823351315 4.195741406387345E-5 78-79 1.2587224219162035E-4 8.39148281277469E-5 0.0 2.0961924066311175 4.195741406387345E-5 80-81 1.2587224219162035E-4 8.39148281277469E-5 0.0 2.331363712459128 4.195741406387345E-5 82-83 1.2587224219162035E-4 8.39148281277469E-5 0.0 2.580422922342281 4.195741406387345E-5 84-85 1.2587224219162035E-4 8.39148281277469E-5 0.0 2.8275940485925597 4.195741406387345E-5 86-87 1.2587224219162035E-4 1.2587224219162035E-4 0.0 3.0822755519602714 4.195741406387345E-5 88 1.2587224219162035E-4 1.2587224219162035E-4 0.0 3.29760100093607 4.195741406387345E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3855 0.0 47.674763 1 GTATCAA 6695 0.0 40.75457 1 ATCAACG 8100 0.0 33.53623 3 TCAACGC 8095 0.0 33.382774 4 TATCAAC 8265 0.0 33.113026 2 CAACGCA 8255 0.0 32.67881 5 AACGCAG 8610 0.0 31.38601 6 ACGCAGA 9780 0.0 27.342915 7 GTACATG 6245 0.0 27.090084 1 CGCAGAG 9930 0.0 26.930445 8 TACATGG 6380 0.0 25.619724 2 GCAGAGT 10925 0.0 24.262646 9 TATAACG 235 1.9645086E-10 24.05354 2 ACATGGG 6680 0.0 23.287502 3 AGAGTAC 9685 0.0 21.157646 10-11 GAGTACT 7575 0.0 21.12611 12-13 GTCTCGC 685 0.0 20.63854 1 CATGGGG 4770 0.0 20.493511 4 GAGTACA 4880 0.0 20.182491 1 CAGAGTA 10790 0.0 19.970934 10-11 >>END_MODULE