FastQCFastQC Report
Wed 25 May 2016
SRR1033074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908122
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19500.034.233144
CAACGCA20700.032.2486085
AACGCAG21200.031.7006286
GTATCAA22550.031.6876531
ATCAACG21200.031.2662833
GGTATCA15200.030.618551
TATCAAC25750.026.8369482
GTACATG46850.025.3865641
ATACCGT2251.1641532E-1025.0649246
ACGCAGA27050.023.977417
TATACCG1000.001756861523.5051485
CGCAGAG27800.023.3507528
TATATCG1452.5301175E-522.6946265
TACATGG48150.022.5532282
GGACCGT1703.4558943E-622.116116
ACACCGT3007.2759576E-1221.9318086
ACATGGG49400.020.6502723
GTACGGC1857.21952E-620.31278894
GCGTATG1909.029196E-619.793811
TGCACCG3354.0017767E-1119.6460955
Produced by FastQC (version 0.11.5)