FastQCFastQC Report
Wed 25 May 2016
SRR1033074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908122
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.14857540555582924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.13227665736257954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.052.4765851
GTATCAA44050.042.2784541
TCAACGC49600.036.5758364
ATCAACG51100.035.5969543
CAACGCA51200.035.3401225
AACGCAG52800.034.2692076
TATCAAC54350.033.6403432
ACGCAGA62750.028.8352857
CGCAGAG64050.028.2500278
GCAGAGT71550.024.9603829
GTACATG47350.024.2961671
GACCGCT2002.3013854E-823.4988867
TCGACCG1602.0408188E-623.4988865
TACATGG49850.022.403272
ACATGGG50050.022.163113
GAGTACT49100.022.1103912-13
TAGACTG7800.021.0887455
AGAGTAC65000.020.93208510-11
TGGTATC12400.020.9011042
GAGTACA36250.020.8103751
Produced by FastQC (version 0.11.5)