##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1908122 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.669707178052555 34.0 31.0 34.0 31.0 34.0 2 32.6768005400074 34.0 31.0 34.0 31.0 34.0 3 32.785640540803996 34.0 31.0 34.0 31.0 34.0 4 36.28368888362484 37.0 37.0 37.0 35.0 37.0 5 36.260129069315276 37.0 37.0 37.0 35.0 37.0 6 36.330303827533044 37.0 37.0 37.0 35.0 37.0 7 36.28405730870458 37.0 37.0 37.0 35.0 37.0 8 36.28782017082765 37.0 37.0 37.0 35.0 37.0 9 38.083390370217415 39.0 39.0 39.0 35.0 39.0 10-11 38.04776764798058 39.0 39.0 39.0 35.0 39.0 12-13 38.01747424955008 39.0 38.0 39.0 35.0 39.0 14-15 39.51237473285251 41.0 39.0 41.0 37.0 41.0 16-17 39.5055405262347 41.0 39.0 41.0 36.0 41.0 18-19 39.52025342195101 41.0 39.5 41.0 36.5 41.0 20-21 39.513011222552855 41.0 39.5 41.0 37.0 41.0 22-23 39.44070871778639 41.0 39.0 41.0 36.0 41.0 24-25 39.38481134854061 41.0 39.0 41.0 36.0 41.0 26-27 39.277016878375704 41.0 39.0 41.0 36.0 41.0 28-29 39.22584981463449 41.0 39.0 41.0 36.0 41.0 30-31 39.22896885000016 41.0 39.0 41.0 36.0 41.0 32-33 39.1982763680729 41.0 39.0 41.0 35.0 41.0 34-35 39.12347716760249 41.0 39.0 41.0 35.0 41.0 36-37 39.04008889368709 41.0 39.0 41.0 35.0 41.0 38-39 38.94929962549564 41.0 39.0 41.0 35.0 41.0 40-41 38.855077400711274 41.0 39.0 41.0 35.0 41.0 42-43 38.738407449838114 41.0 39.0 41.0 35.0 41.0 44-45 38.58068535450039 41.0 38.0 41.0 35.0 41.0 46-47 38.396267114995794 40.5 38.0 41.0 34.5 41.0 48-49 38.20059251976551 40.0 38.0 41.0 34.0 41.0 50-51 38.06875320341152 40.0 38.0 41.0 34.0 41.0 52-53 37.934892003760766 40.0 38.0 41.0 33.5 41.0 54-55 37.783683380832045 40.0 37.5 41.0 33.0 41.0 56-57 37.636034016692854 40.0 37.0 41.0 33.0 41.0 58-59 37.46111385959598 40.0 37.0 41.0 33.0 41.0 60-61 37.273300396934786 40.0 36.0 41.0 33.0 41.0 62-63 37.04266813128301 39.0 36.0 41.0 32.5 41.0 64-65 36.78037541624697 39.0 35.0 41.0 32.0 41.0 66-67 36.50951878339016 39.0 35.0 41.0 32.0 41.0 68-69 36.2069802140534 38.0 35.0 40.5 31.0 41.0 70-71 35.88459542943271 37.0 35.0 40.0 31.0 41.0 72-73 35.54750430003951 37.0 35.0 39.5 31.0 41.0 74-75 35.20572531525762 36.0 35.0 39.0 31.0 41.0 76-77 34.85346062777957 36.0 35.0 39.0 30.5 40.5 78-79 34.496047422544265 35.5 34.5 37.5 30.0 39.5 80-81 34.16114299819404 35.0 34.0 37.0 30.0 39.0 82-83 33.85688284082464 35.0 34.0 37.0 30.0 39.0 84-85 33.620164224300126 35.0 34.0 36.0 30.0 38.0 86-87 33.40069424282095 35.0 34.0 36.0 30.0 37.0 88-89 33.18534139850597 35.0 34.0 36.0 30.0 37.0 90-91 32.99339298011343 35.0 34.0 35.0 29.5 36.5 92-93 32.80689573308206 35.0 34.0 35.0 29.0 36.0 94-95 32.644209332526955 35.0 34.0 35.0 29.0 36.0 96-97 32.49024433448176 35.0 34.0 35.0 29.0 36.0 98-99 32.35905303748922 35.0 34.0 35.0 29.0 36.0 100 32.24776874853914 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08859357887031649 1101 2 0.11996653142285396 1101 3 0.15183540568182252 1101 4 0.13683193608449074 1101 5 0.09502982827861928 1101 6 0.1055767111376511 1101 7 0.1291568942972816 1101 8 0.04711174328132728 1101 9 0.17333608084228302 1101 10-11 0.18184964264828807 1101 12-13 0.15611206079272932 1101 14-15 0.27384934748342005 1101 16-17 0.2471327875578453 1101 18-19 0.19391551342346958 1101 20-21 0.19586495078848287 1101 22-23 0.2519324060556727 1101 24-25 0.30086083793221263 1101 26-27 0.2941873442614522 1101 28-29 0.2757462869810752 1101 30-31 0.35156824549975596 1101 32-33 0.25847259102978626 1101 34-35 0.3226119109677157 1101 36-37 0.3037817340634774 1101 38-39 0.25740470403032134 1101 40-41 0.21370283787207 1101 42-43 0.19388151058091552 1101 44-45 0.1707840262969995 1101 46-47 0.1830702899634744 1101 48-49 0.22688044141899155 1101 50-51 0.24353355890136896 1101 52-53 0.20158283599835158 1101 54-55 0.18786565357163454 1101 56-57 0.11403582749090191 1101 58-59 0.11872960560968693 1101 60-61 0.1656268198220303 1101 62-63 0.13724245263649948 1101 64-65 0.08819525764022274 1101 66-67 0.006372702655042417 1101 68-69 -0.027651864318556818 1101 70-71 0.01586129307671058 1101 72-73 0.0060311835680053605 1101 74-75 -0.02750593741546936 1101 76-77 0.030355284349468548 1101 78-79 0.06511990550659164 1101 80-81 0.016117091385012827 1101 82-83 0.0120408514911432 1101 84-85 0.017106390067027633 1101 86-87 0.11012349942532751 1101 88-89 0.12902753646049092 1101 90-91 0.157247597770521 1101 92-93 0.12612953898401713 1101 94-95 0.13259667922563523 1101 96-97 0.15230904530598366 1101 98-99 0.14033503547847914 1101 100 0.1721695237402585 1102 1 0.10693577029563528 1102 2 0.11116800680753869 1102 3 0.1784275989931814 1102 4 0.09008286841596203 1102 5 0.12614819854057657 1102 6 0.10506260860256589 1102 7 0.1494344750888672 1102 8 0.1613866308105969 1102 9 0.20320322203611596 1102 10-11 0.19623964136577854 1102 12-13 0.19770022112607677 1102 14-15 0.28744634579101813 1102 16-17 0.2737876916339559 1102 18-19 0.2461393565906036 1102 20-21 0.2856990643128867 1102 22-23 0.28639550760750154 1102 24-25 0.2673168655840499 1102 26-27 0.2607938620317043 1102 28-29 0.34966420409095633 1102 30-31 0.324278218114479 1102 32-33 0.23803854766600097 1102 34-35 0.27375378126362904 1102 36-37 0.27537917999077166 1102 38-39 0.24279113122045715 1102 40-41 0.29529983743702104 1102 42-43 0.27820658558236033 1102 44-45 0.21019098363984767 1102 46-47 0.24085880977567342 1102 48-49 0.227779845808179 1102 50-51 0.33329828194298017 1102 52-53 0.3664821278539421 1102 54-55 0.3901298736697498 1102 56-57 0.3944312165051116 1102 58-59 0.3332181026637002 1102 60-61 0.32750727204365404 1102 62-63 0.33312927086517874 1102 64-65 0.35577761149113485 1102 66-67 0.35829176207529656 1102 68-69 0.35106866005031634 1102 70-71 0.30096784793904874 1102 72-73 0.3146486956826564 1102 74-75 0.33585060675930833 1102 76-77 0.26856911831274033 1102 78-79 0.30490418047584456 1102 80-81 0.2791150368609152 1102 82-83 0.34816811442244244 1102 84-85 0.28066189553779 1102 86-87 0.2694589790699453 1102 88-89 0.33424623514454055 1102 90-91 0.2127282357040201 1102 92-93 0.29977486072952786 1102 94-95 0.2388556693402748 1102 96-97 0.18219206065330695 1102 98-99 0.15536557986450106 1102 100 0.1371599426670258 1103 1 0.1253453775719109 1103 2 0.04043801862881935 1103 3 0.06825941886312137 1103 4 0.03354357176985445 1103 5 0.08494000083638298 1103 6 0.08380448602537882 1103 7 0.06526627918091776 1103 8 0.07799124790557244 1103 9 0.05671935431792008 1103 10-11 0.08601912113246613 1103 12-13 0.14464594094965832 1103 14-15 0.18793180404151144 1103 16-17 0.17321777617915757 1103 18-19 0.2428251660811256 1103 20-21 0.24511341041659307 1103 22-23 0.16680474349661267 1103 24-25 0.20184880245308534 1103 26-27 0.14180591937097375 1103 28-29 0.20471549435794145 1103 30-31 0.2595922223235334 1103 32-33 0.15024394115434347 1103 34-35 0.20122071445520362 1103 36-37 0.12700659309709295 1103 38-39 0.08417156579199059 1103 40-41 0.14469786556534814 1103 42-43 0.18911159299796765 1103 44-45 0.24365808007186018 1103 46-47 0.1939016964977327 1103 48-49 0.11582004118695721 1103 50-51 0.1637630741967584 1103 52-53 0.14014334386776284 1103 54-55 0.07347968275733052 1103 56-57 0.07276441633324993 1103 58-59 0.1268012515171293 1103 60-61 0.21223851092327806 1103 62-63 0.2054862473609873 1103 64-65 0.20874811716025476 1103 66-67 0.18655713216382708 1103 68-69 0.1202020778560211 1103 70-71 0.21187968232464272 1103 72-73 0.0974573687866993 1103 74-75 0.1567509657669035 1103 76-77 0.06677329144226007 1103 78-79 0.12654458303040883 1103 80-81 0.17294806798703632 1103 82-83 0.15972288249965771 1103 84-85 0.15636454246025266 1103 86-87 0.14661341908848868 1103 88-89 0.14669040570105807 1103 90-91 0.10998961944891761 1103 92-93 0.18775459949628015 1103 94-95 0.17809009899396955 1103 96-97 0.21012559448716672 1103 98-99 0.27881881603033065 1103 100 0.23623930300724538 1104 1 0.049830640931723735 1104 2 0.06505975161980615 1104 3 0.04501047098504074 1104 4 0.025575091516927273 1104 5 0.057989704208488035 1104 6 0.08470003186923947 1104 7 0.06694614985254788 1104 8 0.10819584257574633 1104 9 0.14347362462716262 1104 10-11 0.16077401601270935 1104 12-13 0.14260286866119287 1104 14-15 0.1975718495005765 1104 16-17 0.18482881693053343 1104 18-19 0.138966909032888 1104 20-21 0.1310802359319112 1104 22-23 0.13270524662004135 1104 24-25 0.14156953948153728 1104 26-27 0.11877248026693366 1104 28-29 0.16149572971895054 1104 30-31 0.05643771986569135 1104 32-33 0.07305012827836066 1104 34-35 0.07571942544695531 1104 36-37 0.04156379189902282 1104 38-39 0.019957317715395106 1104 40-41 0.0832557652041217 1104 42-43 0.026990856662841622 1104 44-45 0.042341541015659345 1104 46-47 0.0899460127282552 1104 48-49 0.05383057453939699 1104 50-51 0.11190851430843196 1104 52-53 0.08769418487197811 1104 54-55 0.10786539319673238 1104 56-57 0.13951298692653324 1104 58-59 0.09785980981542508 1104 60-61 0.09630222929894927 1104 62-63 0.11593159075370352 1104 64-65 0.038165194815057646 1104 66-67 0.08484572961192072 1104 68-69 0.01803308026648409 1104 70-71 -0.03740258101436922 1104 72-73 0.059579823992073955 1104 74-75 -0.008146540455690854 1104 76-77 -0.05610347047196029 1104 78-79 0.0071623998860843585 1104 80-81 0.04080392323909621 1104 82-83 0.0523940964207128 1104 84-85 -0.007929553639328901 1104 86-87 0.02215052993049227 1104 88-89 0.08978821721812835 1104 90-91 0.12478266458211351 1104 92-93 0.09524406383275874 1104 94-95 0.11336372869326539 1104 96-97 0.20349732747814642 1104 98-99 0.2464322672444581 1104 100 0.21828071998032783 1105 1 0.07334752243533416 1105 2 0.014772816359574392 1105 3 0.08138148352534103 1105 4 0.06841226231776432 1105 5 0.046718692542995655 1105 6 0.09994565457561322 1105 7 0.08040225444394622 1105 8 0.11970359770099748 1105 9 0.08924597692767833 1105 10-11 0.12924489930091454 1105 12-13 0.1303817393347515 1105 14-15 0.13518516377779122 1105 16-17 0.16324442909554193 1105 18-19 0.1366993932973699 1105 20-21 0.14376022657152276 1105 22-23 0.10706150124009639 1105 24-25 0.15916769780331919 1105 26-27 0.168714961994489 1105 28-29 0.09732868397114203 1105 30-31 0.1159402906919027 1105 32-33 0.09625303765318449 1105 34-35 0.13100681451105345 1105 36-37 0.14815808140620845 1105 38-39 0.16323112437525822 1105 40-41 0.12162761536793454 1105 42-43 0.13275599730923915 1105 44-45 0.1569907123588763 1105 46-47 0.015984458116513167 1105 48-49 0.0036719892264542864 1105 50-51 0.007982782864090154 1105 52-53 0.0729063299754884 1105 54-55 -0.005767776913003786 1105 56-57 -0.03327734366138202 1105 58-59 -0.022953166131877367 1105 60-61 -0.10015233235932186 1105 62-63 -0.04628866377012031 1105 64-65 -0.07111391861621996 1105 66-67 -0.09159088765755996 1105 68-69 -0.03634342056676587 1105 70-71 -0.0896925409046645 1105 72-73 -0.05071419716939829 1105 74-75 -0.0743544733845738 1105 76-77 -0.08364075346623423 1105 78-79 -0.09228871614336498 1105 80-81 -0.04486932485929884 1105 82-83 -0.042051098288489186 1105 84-85 -0.1507763472690158 1105 86-87 -0.22245398820635387 1105 88-89 -0.17731048348127842 1105 90-91 -0.1794237964913492 1105 92-93 -0.18279459041463042 1105 94-95 -0.19168500328032678 1105 96-97 -0.14753376410055807 1105 98-99 -0.15612702250329136 1105 100 -0.15930869578274098 1106 1 -3.520662002429731E-4 1106 2 0.045317585880155775 1106 3 0.08602375025864717 1106 4 0.04298071412661386 1106 5 0.024785664208827995 1106 6 0.032445366236068196 1106 7 0.04943458691867164 1106 8 0.0013978808888737149 1106 9 0.02756053615262033 1106 10-11 0.15792507307355663 1106 12-13 0.1365810396307765 1106 14-15 0.11876422648203544 1106 16-17 0.16297050652431722 1106 18-19 0.0977303032969914 1106 20-21 0.11668033729390714 1106 22-23 0.11179507549714174 1106 24-25 0.1273109833363577 1106 26-27 0.06918495545879466 1106 28-29 0.05632680015276037 1106 30-31 0.09974521937591874 1106 32-33 0.13337675454219067 1106 34-35 0.06571232371869229 1106 36-37 0.1021198668046921 1106 38-39 0.11952365445868907 1106 40-41 0.13206550702915365 1106 42-43 0.028127186421535555 1106 44-45 0.05051172270065507 1106 46-47 0.010039857755138826 1106 48-49 0.10914659612354427 1106 50-51 0.08884280362453723 1106 52-53 0.03685427507564043 1106 54-55 0.046484154961973445 1106 56-57 0.03617884794985571 1106 58-59 0.05724367366667593 1106 60-61 0.06928079208802984 1106 62-63 0.014099249036000572 1106 64-65 -0.044062863293476084 1106 66-67 -0.032404389637491704 1106 68-69 -0.03897692177778822 1106 70-71 -0.024917061299888132 1106 72-73 0.022552882143195063 1106 74-75 0.026737372372991786 1106 76-77 -0.014833325839461509 1106 78-79 0.010586029156698373 1106 80-81 0.05505955138536933 1106 82-83 0.04629613941863653 1106 84-85 0.05489625278481469 1106 86-87 0.03991097277169331 1106 88-89 0.0031612400203187008 1106 90-91 0.002081143726243795 1106 92-93 0.01701991114240542 1106 94-95 -0.04749600631750894 1106 96-97 -0.07251261920032448 1106 98-99 -0.159183421719014 1106 100 -0.1127226360387894 1107 1 -0.04090646523173547 1107 2 -0.023015663312335732 1107 3 0.0023286113223264238 1107 4 -0.00241772243762739 1107 5 0.02183628036723917 1107 6 0.04590913766599414 1107 7 0.036349201786258334 1107 8 0.0176803703885966 1107 9 0.06121043701491402 1107 10-11 0.08164769141764339 1107 12-13 0.05034831201248835 1107 14-15 0.09477678722429062 1107 16-17 0.05985355867876763 1107 18-19 0.08576733219491217 1107 20-21 0.09139316956360233 1107 22-23 0.15842728896542013 1107 24-25 0.1354178293318995 1107 26-27 0.07441443227106248 1107 28-29 0.14105513908393164 1107 30-31 0.15333278672570572 1107 32-33 0.12172040255235572 1107 34-35 0.15285364864982398 1107 36-37 0.14024234814770153 1107 38-39 0.14306366243722124 1107 40-41 0.16945992265123522 1107 42-43 0.1773101293908752 1107 44-45 0.19858932728028833 1107 46-47 0.20038041532182405 1107 48-49 0.18721815741123038 1107 50-51 0.24203109795116973 1107 52-53 0.248025010694235 1107 54-55 0.16125535127640944 1107 56-57 0.24059592022486243 1107 58-59 0.23436706670040763 1107 60-61 0.15309508929920668 1107 62-63 0.19204697480814303 1107 64-65 0.17629147357277475 1107 66-67 0.13589980621862452 1107 68-69 0.14462605819372243 1107 70-71 0.12316277053722757 1107 72-73 0.1629096986894396 1107 74-75 0.259161093753967 1107 76-77 0.3431594598261114 1107 78-79 0.23592694803404157 1107 80-81 0.24431704313708735 1107 82-83 0.3304018856439299 1107 84-85 0.3021275242716541 1107 86-87 0.19247650116130188 1107 88-89 0.23899489259388673 1107 90-91 0.23234949855508802 1107 92-93 0.25303013573905275 1107 94-95 0.24702216859842707 1107 96-97 0.2362682938960461 1107 98-99 0.2694975667081607 1107 100 0.16449840986152253 1108 1 -0.01845784104672532 1108 2 -0.049485660755181016 1108 3 0.019455484629027353 1108 4 -0.02956240210478711 1108 5 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50-51 -0.2063485065131161 2214 52-53 -0.17707223330667432 2214 54-55 -0.23380051373608524 2214 56-57 -0.32368093634784145 2214 58-59 -0.32071191698692303 2214 60-61 -0.31447104982681395 2214 62-63 -0.28372755300643604 2214 64-65 -0.3278600913293346 2214 66-67 -0.3466188456506174 2214 68-69 -0.3020514307644149 2214 70-71 -0.24915614801980723 2214 72-73 -0.20563907624804045 2214 74-75 -0.28801767858543315 2214 76-77 -0.22606512483314134 2214 78-79 -0.25888747300714954 2214 80-81 -0.20074585452223914 2214 82-83 -0.1866272540821612 2214 84-85 -0.17309514038286977 2214 86-87 -0.20996164686331298 2214 88-89 -0.17566823748059335 2214 90-91 -0.16640311258376528 2214 92-93 -0.21422828670007021 2214 94-95 -0.1841521728663693 2214 96-97 -0.08028941281003199 2214 98-99 -0.07937760903123348 2214 100 -0.026718029211494354 2215 1 -0.12111097372331159 2215 2 -0.1079818942844426 2215 3 -0.12346118936140016 2215 4 -0.07546531708758408 2215 5 -0.1194852119676213 2215 6 -0.12397964685511909 2215 7 -0.19949494538997214 2215 8 -0.17491871110954804 2215 9 -0.188239570984436 2215 10-11 -0.13294410134918166 2215 12-13 -0.15918303414532176 2215 14-15 -0.2903829003790719 2215 16-17 -0.26456256251086785 2215 18-19 -0.21423428730503957 2215 20-21 -0.1788904784925549 2215 22-23 -0.27770454029413827 2215 24-25 -0.27788842399912284 2215 26-27 -0.2574671420399355 2215 28-29 -0.23081875977878497 2215 30-31 -0.34408607387263856 2215 32-33 -0.2897578770086682 2215 34-35 -0.24703131777744858 2215 36-37 -0.3650992043932959 2215 38-39 -0.3570454820447537 2215 40-41 -0.3007924641481665 2215 42-43 -0.2962913342444793 2215 44-45 -0.3469949833967263 2215 46-47 -0.40650815228445225 2215 48-49 -0.29994239785150256 2215 50-51 -0.2224889460599755 2215 52-53 -0.10688001299462968 2215 54-55 -0.15911290338358697 2215 56-57 -0.11880959569384686 2215 58-59 -0.13187740123496638 2215 60-61 -0.12558831612641796 2215 62-63 -0.10704296436736627 2215 64-65 -0.2341775139845481 2215 66-67 -0.1271530689827074 2215 68-69 -0.09333904501750112 2215 70-71 -0.1426012457441459 2215 72-73 -0.1914670244830532 2215 74-75 -0.1386337852890165 2215 76-77 -0.14993666093165814 2215 78-79 -0.18998431514647507 2215 80-81 -0.14784649102405467 2215 82-83 -0.1036510945795115 2215 84-85 -0.12853893431379504 2215 86-87 -0.1480072680138207 2215 88-89 -0.13749767291979254 2215 90-91 -0.14155829595097202 2215 92-93 -0.12053388309426083 2215 94-95 -0.13723913821043965 2215 96-97 -0.1155862789338471 2215 98-99 -0.12787000972488016 2215 100 -0.1392957350081474 2216 1 -0.17245708629892675 2216 2 -0.14526938668617362 2216 3 -0.1485377458441164 2216 4 -0.14391272292741064 2216 5 -0.11591340495154867 2216 6 -0.1687759291460793 2216 7 -0.15315372363075852 2216 8 -0.1900927511075139 2216 9 -0.18562052637108195 2216 10-11 -0.2604916199245295 2216 12-13 -0.25925284830194784 2216 14-15 -0.3363042043315403 2216 16-17 -0.3315802891859576 2216 18-19 -0.3229101403670924 2216 20-21 -0.3114735296045481 2216 22-23 -0.33676062229306325 2216 24-25 -0.3469547849371608 2216 26-27 -0.39507776878743783 2216 28-29 -0.2867898593957463 2216 30-31 -0.34543779070795466 2216 32-33 -0.3945715344174019 2216 34-35 -0.3234573532170302 2216 36-37 -0.4151946298254643 2216 38-39 -0.43068442158875087 2216 40-41 -0.35250929945906506 2216 42-43 -0.3517611490896897 2216 44-45 -0.3561015037329227 2216 46-47 -0.265131769055607 2216 48-49 -0.36809708153982257 2216 50-51 -0.3893772583285582 2216 52-53 -0.3810739846474007 2216 54-55 -0.26462695936420744 2216 56-57 -0.36100860008659197 2216 58-59 -0.31163060723492464 2216 60-61 -0.33884451342390065 2216 62-63 -0.3934746882261706 2216 64-65 -0.39413299359250686 2216 66-67 -0.3472137174012957 2216 68-69 -0.3378491849021259 2216 70-71 -0.38282778024974107 2216 72-73 -0.306264565341543 2216 74-75 -0.37550392292428114 2216 76-77 -0.3268521988086164 2216 78-79 -0.3319554384811241 2216 80-81 -0.39210101545958764 2216 82-83 -0.32872715375590644 2216 84-85 -0.4030123538647459 2216 86-87 -0.5291561833313452 2216 88-89 -0.6353105579735541 2216 90-91 -0.5452786062854216 2216 92-93 -0.5180610082850094 2216 94-95 -0.44313814753799363 2216 96-97 -0.6086167986227835 2216 98-99 -0.6596667430344141 2216 100 -0.6429656643868213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 4.0 4 7.0 5 18.0 6 45.0 7 119.0 8 250.0 9 407.0 10 811.0 11 1243.0 12 1811.0 13 2853.0 14 3552.0 15 4631.0 16 5837.0 17 7825.0 18 9466.0 19 5178.0 20 3605.0 21 3353.0 22 3593.0 23 3942.0 24 4667.0 25 5639.0 26 7251.0 27 9394.0 28 12133.0 29 15857.0 30 20100.0 31 25851.0 32 33297.0 33 43222.0 34 59668.0 35 90573.0 36 163735.0 37 364072.0 38 655325.0 39 337288.0 40 1490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72112360292834 19.865851759535637 13.633301228495046 23.779723409040983 2 15.565793655588331 19.868720006934357 40.52611809946075 24.039368238016564 3 18.196721397391812 25.087824392094838 30.17574312040078 26.539711090112565 4 12.123330711141554 16.792648918568627 39.06187027435533 32.022150095934485 5 12.362442993567484 37.79734796275702 36.1660547198863 13.6741543237892 6 30.881479738444263 38.50012656430069 17.35623684242247 13.262156854832583 7 27.0870520857681 32.50929447907419 22.460880383958678 17.942773051199033 8 24.93734651074307 36.86320628641685 20.005460889653342 18.193986313186738 9 24.743753282022848 16.466924022677794 21.37337130435056 37.4159513909488 10-11 23.858144558114137 26.74901335692385 28.874098814456072 20.518743270505944 12-13 24.74663569729818 23.910106376845924 29.34319713309736 22.000060792758536 14-15 21.738031013788973 25.957133700780716 26.61576662671458 25.68906865871573 16-17 20.969042827495127 29.530820912730853 27.16715929815735 22.33297696161667 18-19 20.79294653193656 28.06186327734602 29.828505783992647 21.316684406724768 20-21 21.882694083502 27.342250652736038 29.63827784596582 21.13677741779614 22-23 21.500650640761922 27.11958740518804 29.805740627287918 21.574021326762114 24-25 21.41145642261936 27.209868455531684 29.73497196163723 21.64370316021173 26-27 21.20667002424901 27.192512626308947 29.81019527608079 21.790622073361252 28-29 21.204154457871862 27.30131087212066 29.762792572161377 21.731742097846098 30-31 21.419722030061003 27.767121564681467 29.398151059681783 21.41500534557575 32-33 21.127868134217834 27.450131595359206 29.88042693286907 21.54157333755389 34-35 21.211431973427274 27.227347098351153 29.874164230589027 21.687056697632542 36-37 21.560309036843556 27.626247168682088 29.30677388552724 21.50666990894712 38-39 21.397347758686287 27.821229460170787 29.356220409386818 21.425202371756104 40-41 21.283104875659124 27.51973603358067 29.529461305268036 21.66769778549217 42-43 21.27112417340191 27.452725769107005 29.402077016039858 21.87407304145123 44-45 21.50114091237353 27.669430990261628 29.229734786350136 21.599693311014704 46-47 21.55215966274693 27.966372171171443 28.859947110300077 21.62152105578155 48-49 21.528785895241498 27.80293922506003 28.724683222561243 21.943591657137226 50-51 21.59413811066588 27.82041714313865 28.573199197954846 22.012245548240625 52-53 21.63428229431871 27.812687029445705 28.65319932373297 21.89983135250262 54-55 21.719182526064895 28.16541604782084 28.411652923660018 21.703748502454246 56-57 21.512356128172097 27.93235967092251 28.483005803612137 22.072278397293253 58-59 21.39829109459458 27.515876867412043 28.898283233462013 22.187548804531364 60-61 21.638134249277563 27.951121573987407 28.482665154534143 21.928079022200887 62-63 21.483767809395836 27.91459351131636 28.597856950446566 22.003781728841236 64-65 21.50501907110761 27.626771244186692 28.75211857522737 22.116091109478326 66-67 21.367612072920934 27.84846247998359 28.514326786842453 22.269598660253028 68-69 21.829726284984773 27.923800230174685 28.446226539686265 21.800246945154278 70-71 21.83641821644528 27.713138887345778 28.453552760253274 21.996890135955667 72-73 21.596534588604243 27.852607338974504 28.590456335418114 21.960401737003135 74-75 21.722439743013933 27.86296347345213 28.42175985879287 21.992836924741066 76-77 21.70746943853695 27.93235967092251 28.22204240609353 22.138128484447012 78-79 21.80741063726533 28.02797724673789 28.353794987951503 21.810817128045272 80-81 21.691039671467546 27.812582214344783 28.37677569882848 22.119602415359186 82-83 21.53786006009059 27.92110974210728 28.482290674743034 22.058739523059096 84-85 21.55365327793506 27.85500612644265 28.342868013680466 22.248472581941826 86-87 21.675093836112516 27.975608375600203 28.3051484345275 22.04414935375979 88-89 21.386189621554028 27.910210240191773 28.35505543663144 22.348544701622757 90-91 21.390932610512298 27.697430846041794 28.435293183538736 22.476343359907176 92-93 21.509433171200566 27.98952050382919 28.265972505734744 22.235073819235502 94-95 21.275255999257904 27.739895635002366 28.461027686544444 22.52382067919529 96-97 21.331899808004383 27.646477560463424 28.46820411646773 22.553418515064465 98-99 21.314236690688578 27.756654590201137 28.391311024102794 22.53779769500749 100 21.4024574948562 27.589430864483504 28.305370411325903 22.702741229334393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 17.0 2 69.5 3 123.0 4 82.0 5 63.0 6 160.5 7 355.5 8 628.5 9 760.0 10 658.5 11 539.5 12 542.5 13 666.0 14 822.0 15 970.0 16 1142.0 17 1331.5 18 1539.5 19 1827.0 20 2248.0 21 2845.0 22 3775.0 23 5219.5 24 7064.5 25 9428.5 26 12628.5 27 16697.0 28 21552.5 29 26962.0 30 32425.5 31 38172.5 32 43786.5 33 49129.5 34 54235.0 35 58457.5 36 61781.5 37 64583.5 38 66790.0 39 67790.5 40 68313.0 41 68874.5 42 68315.5 43 67051.5 44 65753.5 45 64886.0 46 64421.0 47 62981.0 48 60939.5 49 58940.5 50 56795.5 51 54742.0 52 51568.0 53 48742.0 54 47204.5 55 44991.5 56 41805.5 57 37904.0 58 34602.0 59 31887.0 60 28748.5 61 24624.0 62 20745.5 63 17276.5 64 13917.0 65 11245.0 66 8669.0 67 6411.0 68 4881.5 69 3811.5 70 2772.0 71 1906.5 72 1418.5 73 1012.0 74 749.5 75 539.0 76 330.5 77 207.0 78 121.0 79 65.0 80 38.5 81 18.5 82 6.0 83 1.5 84 2.5 85 2.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04344585933184566 2 0.2388212074489996 3 0.0027776001744123278 4 0.002567969972569888 5 2.0963020184243983E-4 6 5.240755046060996E-5 7 0.0 8 4.1926040368487965E-4 9 0.0 10-11 5.502792798364046E-4 12-13 0.0 14-15 1.5722265138182987E-4 16-17 1.0481510092121991E-4 18-19 5.764830550667096E-4 20-21 0.0 22-23 1.5722265138182987E-4 24-25 0.0011529661101334192 26-27 1.5722265138182987E-4 28-29 1.5722265138182987E-4 30-31 1.0481510092121991E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.620377523030498E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.620377523030498E-5 68-69 3.1444530276365974E-4 70-71 0.0 72-73 4.1926040368487965E-4 74-75 1.5722265138182987E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5722265138182987E-4 84-85 0.0 86-87 4.1926040368487965E-4 88-89 0.0022273208945759234 90-91 0.0022273208945759234 92-93 7.861132569091494E-4 94-95 5.764830550667096E-4 96-97 2.882415275333548E-4 98-99 1.5722265138182987E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1908122.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.15900404302373 #Duplication Level Percentage of deduplicated Percentage of total 1 79.82150915643616 56.0019758362666 2 13.131408752987838 18.42573119582942 3 3.5958410665611824 7.568418837808104 4 1.4079887504544704 3.951323537426685 5 0.650787182667726 2.2829290289966506 6 0.36993940587922136 1.5572748163652455 7 0.229677018612367 1.1279737614190286 8 0.1571238489559536 0.8818922203324959 9 0.11682690939279723 0.7376813647580905 >10 0.4990083225028487 5.892742580167677 >50 0.015700112496366075 0.7211296901557331 >100 0.0038904911620854235 0.4595099005207463 >500 7.474547275793259E-5 0.04525630619638765 >1k 2.2423641827379776E-4 0.346160923757074 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.14857540555582924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2524 0.13227665736257954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5722265138182987E-4 0.0 10-11 0.0 0.0 0.0 1.8342642661213488E-4 0.0 12-13 0.0 0.0 0.0 2.0963020184243985E-4 0.0 14-15 0.0 0.0 0.0 3.930566284545747E-4 0.0 16-17 0.0 2.6203775230304982E-5 0.0 4.7166795414548966E-4 0.0 18-19 0.0 5.2407550460609963E-5 0.0 4.7166795414548966E-4 0.0 20-21 0.0 5.2407550460609963E-5 0.0 4.7166795414548966E-4 0.0 22-23 0.0 5.2407550460609963E-5 0.0 4.978717293757946E-4 0.0 24-25 0.0 5.2407550460609963E-5 0.0 6.288906055273195E-4 0.0 26-27 0.0 5.2407550460609963E-5 0.0 7.337057064485395E-4 0.0 28-29 0.0 5.2407550460609963E-5 0.0 0.0010743547844425042 0.0 30-31 0.0 5.2407550460609963E-5 0.0 0.002803803949642633 0.0 32-33 0.0 5.2407550460609963E-5 0.0 0.0071536306378732605 0.0 34-35 0.0 1.0481510092121993E-4 0.0 0.012735034761928221 0.0 36-37 0.0 2.0963020184243983E-4 0.0 0.02329515617974113 0.0 38-39 0.0 3.1444530276365974E-4 0.0 0.04292178382723956 0.0 40-41 0.0 3.1444530276365974E-4 0.0 0.0699116723144537 0.0 42-43 0.0 3.1444530276365974E-4 0.0 0.09637748529706172 0.0 44-45 0.0 3.1444530276365974E-4 0.0 0.12433691346779713 0.0 46-47 0.0 3.1444530276365974E-4 0.0 0.159292749625024 0.0 48-49 0.0 3.1444530276365974E-4 0.0 0.20009202765860884 0.0 50-51 0.0 3.1444530276365974E-4 0.0 0.24539835503180615 0.0 52-53 0.0 3.1444530276365974E-4 0.0 0.2921982975931309 0.0 54-55 0.0 3.1444530276365974E-4 0.0 0.3441603838748256 0.0 56-57 0.0 3.1444530276365974E-4 0.0 0.4071804633037091 0.0 58-59 0.0 3.1444530276365974E-4 0.0 0.47612259593464146 0.0 60-61 0.0 3.1444530276365974E-4 0.0 0.5530830837860472 0.0 62-63 0.0 3.1444530276365974E-4 0.0 0.6325853378347925 0.0 64-65 0.0 3.1444530276365974E-4 0.0 0.7164112147965381 0.0 66-67 0.0 3.1444530276365974E-4 0.0 0.8147801870111031 0.0 68-69 0.0 3.1444530276365974E-4 0.0 0.9278756809050994 0.0 70-71 0.0 3.1444530276365974E-4 0.0 1.0511644433636844 0.0 72-73 0.0 3.1444530276365974E-4 0.0 1.1862711084511368 0.0 74-75 0.0 3.1444530276365974E-4 0.0 1.319464897946777 0.0 76-77 0.0 3.1444530276365974E-4 0.0 1.4617776012225634 0.0 78-79 0.0 3.1444530276365974E-4 0.0 1.6316304722654 0.0 80-81 0.0 3.1444530276365974E-4 0.0 1.8162884763133593 0.0 82-83 0.0 3.1444530276365974E-4 0.0 2.011506601779131 0.0 84-85 0.0 3.1444530276365974E-4 0.0 2.2114414067863586 0.0 86-87 0.0 3.1444530276365974E-4 0.0 2.411061766490822 0.0 88 0.0 3.1444530276365974E-4 0.0 2.58442594341452 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2345 0.0 52.476585 1 GTATCAA 4405 0.0 42.278454 1 TCAACGC 4960 0.0 36.575836 4 ATCAACG 5110 0.0 35.596954 3 CAACGCA 5120 0.0 35.340122 5 AACGCAG 5280 0.0 34.269207 6 TATCAAC 5435 0.0 33.640343 2 ACGCAGA 6275 0.0 28.835285 7 CGCAGAG 6405 0.0 28.250027 8 GCAGAGT 7155 0.0 24.960382 9 GTACATG 4735 0.0 24.296167 1 GACCGCT 200 2.3013854E-8 23.498886 7 TCGACCG 160 2.0408188E-6 23.498886 5 TACATGG 4985 0.0 22.40327 2 ACATGGG 5005 0.0 22.16311 3 GAGTACT 4910 0.0 22.11039 12-13 TAGACTG 780 0.0 21.088745 5 AGAGTAC 6500 0.0 20.932085 10-11 TGGTATC 1240 0.0 20.901104 2 GAGTACA 3625 0.0 20.810375 1 >>END_MODULE