FastQCFastQC Report
Wed 25 May 2016
SRR1033073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3891267
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49300.033.5697631
GGTATCA29850.033.549631
TCAACGC48250.031.6680854
CAACGCA49450.030.5196885
ATCAACG50200.030.3442963
GTACATG114500.028.9902321
AACGCAG52900.028.5211966
TACATGG116050.027.1839832
TATCAAC60300.026.197372
ACATGGG121650.025.3142433
ACGCAGA65050.023.266287
CGCAGAG67000.022.330938
CATGGGG84500.021.5879174
GAGTACA81050.019.317181
GCAGAGT80050.017.6354739
TAGACCG1600.001276506917.6308255
CATGGGA43000.017.1659154
GTATAGG14650.017.009471
AGTACAT85400.016.7910462
GTATTAG19550.016.353651
Produced by FastQC (version 0.11.5)