##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033073_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3891267
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66114430081513	33.0	31.0	34.0	30.0	34.0
2	31.68602771282464	34.0	31.0	34.0	30.0	34.0
3	31.865034190663348	34.0	31.0	34.0	30.0	34.0
4	35.22608908615112	37.0	35.0	37.0	33.0	37.0
5	35.31940419405813	37.0	35.0	37.0	33.0	37.0
6	35.348065038970596	37.0	35.0	37.0	33.0	37.0
7	35.28465073201094	37.0	35.0	37.0	33.0	37.0
8	35.308923802967	37.0	36.0	37.0	33.0	37.0
9	36.95068213001061	39.0	38.0	39.0	34.0	39.0
10-11	36.945686199379274	39.0	38.0	39.0	34.0	39.0
12-13	36.87609536945165	39.0	38.0	39.0	34.0	39.0
14-15	38.27753569724206	41.0	39.0	41.0	34.0	41.0
16-17	38.27294593252017	41.0	39.0	41.0	34.0	41.0
18-19	38.27522590971013	41.0	39.0	41.0	34.0	41.0
20-21	38.299394901454974	41.0	39.0	41.0	34.0	41.0
22-23	38.26160900806858	40.0	39.0	41.0	34.0	41.0
24-25	38.27388110350691	40.5	39.0	41.0	34.0	41.0
26-27	38.18543343337787	40.0	38.0	41.0	33.5	41.0
28-29	38.18903701545024	40.0	38.5	41.0	33.5	41.0
30-31	38.32769429597096	40.5	39.0	41.0	34.0	41.0
32-33	38.31533585333517	41.0	39.0	41.0	34.0	41.0
34-35	38.27336880250057	41.0	39.0	41.0	33.5	41.0
36-37	38.199609664410076	41.0	38.0	41.0	33.0	41.0
38-39	38.11499827691084	41.0	38.0	41.0	33.0	41.0
40-41	38.04590818363273	40.0	38.0	41.0	33.0	41.0
42-43	37.922002782127265	40.0	38.0	41.0	33.0	41.0
44-45	37.775980034266475	40.0	38.0	41.0	33.0	41.0
46-47	37.647055958894626	40.0	38.0	41.0	32.0	41.0
48-49	37.5286946385329	40.0	38.0	41.0	32.0	41.0
50-51	37.40057826923724	40.0	37.0	41.0	32.0	41.0
52-53	37.24802487210464	40.0	37.0	41.0	31.0	41.0
54-55	37.0903300904307	40.0	37.0	41.0	31.0	41.0
56-57	36.91723466932493	40.0	36.0	41.0	31.0	41.0
58-59	36.720431417325	39.5	36.0	41.0	31.0	41.0
60-61	36.49646606105415	39.0	35.0	41.0	31.0	41.0
62-63	36.244833752091544	39.0	35.0	41.0	30.0	41.0
64-65	35.96488829987765	38.5	35.0	40.5	30.0	41.0
66-67	35.65984922134616	38.0	35.0	40.0	30.0	41.0
68-69	35.340081777991585	37.0	35.0	40.0	29.5	41.0
70-71	35.00308254355201	37.0	34.5	39.5	29.0	41.0
72-73	34.64405937706151	36.0	34.0	39.0	29.0	41.0
74-75	34.28303673327993	36.0	34.0	39.0	29.0	40.5
76-77	33.91730726778707	35.0	34.0	38.5	28.5	40.0
78-79	33.531881518281835	35.0	34.0	37.0	28.0	39.0
80-81	33.17949732053853	35.0	34.0	37.0	27.5	39.0
82-83	32.95955803084188	35.0	34.0	36.0	27.5	38.5
84-85	32.74145259114833	35.0	34.0	36.0	27.5	37.0
86-87	32.57380462456059	35.0	34.0	36.0	27.5	37.0
88-89	32.36052614739621	35.0	34.0	35.0	27.0	37.0
90-91	32.15671386723142	35.0	33.0	35.0	27.0	36.0
92-93	31.947433830677774	35.0	33.0	35.0	26.5	36.0
94-95	31.756865694386946	35.0	33.0	35.0	26.0	36.0
96-97	31.58669926787342	35.0	33.0	35.0	25.5	35.5
98-99	31.416616233221724	35.0	33.0	35.0	25.0	35.0
100	31.28733520470325	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0044467570165700465
1101	2	0.029274195441040263
1101	3	0.05416305444227021
1101	4	-0.028100010466161507
1101	5	0.013698651620927649
1101	6	0.09546039666157213
1101	7	0.13572984143788602
1101	8	0.1638954289105854
1101	9	0.23300749299618673
1101	10-11	0.1624779340777991
1101	12-13	0.14748115375113713
1101	14-15	0.25772258428130357
1101	16-17	0.2693190359304296
1101	18-19	0.21964912561990957
1101	20-21	0.28203796345655263
1101	22-23	0.23722155582997573
1101	24-25	0.2002161496136594
1101	26-27	0.11684882494229498
1101	28-29	0.1452497484731623
1101	30-31	0.15403343944869619
1101	32-33	0.15815156034708622
1101	34-35	0.18414014838541704
1101	36-37	0.16837476397402185
1101	38-39	0.23279032941820788
1101	40-41	0.20749784038282826
1101	42-43	0.2288991877671478
1101	44-45	0.24316568628407964
1101	46-47	0.19414913583957372
1101	48-49	0.1299048249564123
1101	50-51	0.1530159413597616
1101	52-53	0.08692826846356638
1101	54-55	0.12894483034593662
1101	56-57	0.21179170020231197
1101	58-59	0.2648951952223584
1101	60-61	0.28710778819258564
1101	62-63	0.2579707744102606
1101	64-65	0.2197502550040511
1101	66-67	0.22078410504524015
1101	68-69	0.22194747823669303
1101	70-71	0.18875566871250982
1101	72-73	0.16892116177546512
1101	74-75	0.14969827104268774
1101	76-77	0.19764649579766314
1101	78-79	0.21808050349716268
1101	80-81	0.15280199116241988
1101	82-83	0.17586952586324145
1101	84-85	0.1523625694948052
1101	86-87	0.16498629056287228
1101	88-89	0.2536706353428002
1101	90-91	0.2591087895169295
1101	92-93	0.29040050938892037
1101	94-95	0.2652427706683014
1101	96-97	0.2692728369336024
1101	98-99	0.3129280931289564
1101	100	0.2964875167598571
1102	1	-0.08797413220337802
1102	2	-0.054846047725693126
1102	3	0.005871193788962614
1102	4	-0.11243491272907846
1102	5	-0.04350042576140112
1102	6	0.015240929504507505
1102	7	0.0935498969198818
1102	8	0.13014208432689145
1102	9	0.1391215860094448
1102	10-11	0.17330174987984748
1102	12-13	0.11304758212853727
1102	14-15	0.18321817894283754
1102	16-17	0.16130888221444906
1102	18-19	0.14704115957163566
1102	20-21	0.20034266768982434
1102	22-23	0.11103873026131339
1102	24-25	0.1336771300227042
1102	26-27	-0.006001011176650195
1102	28-29	0.03520326739638335
1102	30-31	0.09936200131792816
1102	32-33	0.07472328043086662
1102	34-35	0.04548068876223965
1102	36-37	0.09352716909290137
1102	38-39	0.1742074201817161
1102	40-41	0.07143624798713688
1102	42-43	0.13682472718330985
1102	44-45	0.08545381217199122
1102	46-47	0.061881841727270626
1102	48-49	0.05567205612854309
1102	50-51	0.039234126998231034
1102	52-53	0.07076769437352937
1102	54-55	0.03563910865884168
1102	56-57	0.07777239792009283
1102	58-59	0.010622504345654704
1102	60-61	-0.018796662008497833
1102	62-63	0.03972039747910827
1102	64-65	-0.008890644368342748
1102	66-67	-0.012020783276184943
1102	68-69	0.06232581962605366
1102	70-71	0.05127569835590151
1102	72-73	0.06648570857313274
1102	74-75	0.031153636999533774
1102	76-77	0.010922895885109085
1102	78-79	-0.10109315362942084
1102	80-81	-0.05686665961808757
1102	82-83	-0.019204388608166312
1102	84-85	0.01569220844540098
1102	86-87	-0.021041086990102542
1102	88-89	-0.011014062814147962
1102	90-91	-4.780390463565709E-4
1102	92-93	0.08761883794416647
1102	94-95	0.06530164102007774
1102	96-97	0.06508158968664546
1102	98-99	0.09962594909345768
1102	100	0.07529797599592314
1103	1	-0.1542642242817429
1103	2	-0.1430421077123043
1103	3	-0.10849465942835579
1103	4	-0.15259627599541403
1103	5	-0.12089744813879832
1103	6	-0.10048421024114162
1103	7	-0.06978283341170766
1103	8	-0.08022478193334592
1103	9	-0.037822099657802255
1103	10-11	0.04491800560082737
1103	12-13	-0.011105893875004824
1103	14-15	0.04496848195121572
1103	16-17	0.05916602507159752
1103	18-19	0.10405431103963991
1103	20-21	0.05151327497287639
1103	22-23	-0.01866549738390688
1103	24-25	-0.037117475523700705
1103	26-27	-0.05001664098182346
1103	28-29	-0.048592658723841
1103	30-31	-0.017873748243381726
1103	32-33	-0.0594471097715612
1103	34-35	-0.11693297646324652
1103	36-37	-0.020713410512364305
1103	38-39	0.02549181389361621
1103	40-41	0.01941685545109806
1103	42-43	-0.02058767173852516
1103	44-45	0.016873429024677478
1103	46-47	0.04958702814526106
1103	48-49	-0.09834248827348802
1103	50-51	-0.04893054674767683
1103	52-53	0.028349416184703102
1103	54-55	-0.019229376661080266
1103	56-57	-0.043902017318970366
1103	58-59	-0.027551395326909756
1103	60-61	-0.12342183612524593
1103	62-63	-0.10335789626716263
1103	64-65	-0.10999205720143124
1103	66-67	-0.07599037389830698
1103	68-69	-0.058760488013376744
1103	70-71	-0.05234828181739459
1103	72-73	-0.07465079896137894
1103	74-75	-0.05500539265005955
1103	76-77	0.0022100935551137013
1103	78-79	-0.030033076565800343
1103	80-81	-0.05567181166497903
1103	82-83	-0.04211218361169955
1103	84-85	-0.06612855531660955
1103	86-87	-0.1118108187146305
1103	88-89	-0.10818812529718258
1103	90-91	-0.1186272457975619
1103	92-93	-0.05080578985301187
1103	94-95	-0.03343377387527369
1103	96-97	-0.03587682128316061
1103	98-99	-0.15231041010261492
1103	100	-0.1612084731077168
1104	1	0.003133715514998414
1104	2	0.05295445170680324
1104	3	0.12957219009113885
1104	4	0.0068356827176145885
1104	5	-0.03587219648586881
1104	6	0.025845145928066415
1104	7	0.05548394978332993
1104	8	0.16487992088475067
1104	9	0.18570228494110808
1104	10-11	0.1552267942814538
1104	12-13	0.13062503611815401
1104	14-15	0.22965952055066907
1104	16-17	0.23583940478598464
1104	18-19	0.24692574523680122
1104	20-21	0.22464413449630882
1104	22-23	0.20776196370229627
1104	24-25	0.1335355785099921
1104	26-27	0.10241788089488324
1104	28-29	0.08812991162498207
1104	30-31	0.1280999776017353
1104	32-33	0.08322704372809397
1104	34-35	0.15081437574031042
1104	36-37	0.27983504433518647
1104	38-39	0.20732478681803457
1104	40-41	0.2755726626403856
1104	42-43	0.2720002254309435
1104	44-45	0.24906210136116158
1104	46-47	0.22112185193639533
1104	48-49	0.23252940190427296
1104	50-51	0.25158820027291284
1104	52-53	0.2917969058733263
1104	54-55	0.3443714537763114
1104	56-57	0.30691066446875226
1104	58-59	0.2919545911456538
1104	60-61	0.28448200032971016
1104	62-63	0.318378733483506
1104	64-65	0.24803558489525557
1104	66-67	0.2703395004306799
1104	68-69	0.27253090431647564
1104	70-71	0.26218927785733825
1104	72-73	0.29960802529955544
1104	74-75	0.28212659300727694
1104	76-77	0.26222401478101887
1104	78-79	0.286221871860036
1104	80-81	0.3318450285276384
1104	82-83	0.23774825868948568
1104	84-85	0.24212496989704135
1104	86-87	0.23396320835403372
1104	88-89	0.23960827936487306
1104	90-91	0.25764304015753936
1104	92-93	0.2655817038427095
1104	94-95	0.25586633044756013
1104	96-97	0.28068639766352277
1104	98-99	0.24288334710843884
1104	100	0.20576388479560492
1105	1	-0.01634143495907736
1105	2	-0.028698672693277416
1105	3	-0.007681285340481736
1105	4	0.020579702013186818
1105	5	0.016302728434475
1105	6	0.01586948475527805
1105	7	0.043552394963079166
1105	8	0.11035924754903448
1105	9	0.14440078397104372
1105	10-11	0.13502839733934024
1105	12-13	0.19899597017694504
1105	14-15	0.20553736224925245
1105	16-17	0.14904372801381527
1105	18-19	0.252315599355704
1105	20-21	0.23862529867650295
1105	22-23	0.22882729588491202
1105	24-25	0.1569140468188337
1105	26-27	0.20131956389668204
1105	28-29	0.12948239160623132
1105	30-31	0.22482705369357348
1105	32-33	0.18455498369188916
1105	34-35	0.16979128831640367
1105	36-37	0.21564520044453417
1105	38-39	0.19661439186342022
1105	40-41	0.23497944867041554
1105	42-43	0.18094028904080517
1105	44-45	0.18043000938892817
1105	46-47	0.06724311825114171
1105	48-49	0.07931758141944556
1105	50-51	0.06754183358045651
1105	52-53	0.10466304777526858
1105	54-55	0.13395245368143094
1105	56-57	0.10907050012098551
1105	58-59	0.07839394053016235
1105	60-61	0.09737150625969093
1105	62-63	0.11864738086593007
1105	64-65	0.1256098302598616
1105	66-67	0.047969248341900084
1105	68-69	0.13240021373162136
1105	70-71	-0.008755015680002032
1105	72-73	0.03988368967841183
1105	74-75	-0.022976104226984262
1105	76-77	-0.010576453434111954
1105	78-79	0.012018718261408878
1105	80-81	0.04227853378992563
1105	82-83	0.019791731098059984
1105	84-85	0.005003319503636305
1105	86-87	-0.022820764338085553
1105	88-89	-0.016305513082862433
1105	90-91	0.04936042082674419
1105	92-93	0.026492672488057423
1105	94-95	0.11489790965725888
1105	96-97	0.1787397003570952
1105	98-99	0.18862302043519463
1105	100	0.1927766653083438
1106	1	-0.14182314528468964
1106	2	-0.1256771473890801
1106	3	-0.14228804971315512
1106	4	-0.13027640591870693
1106	5	-0.07230772316949441
1106	6	-0.10875150363803243
1106	7	-0.130649252731196
1106	8	-0.05734019447939431
1106	9	-0.08408997401247831
1106	10-11	-0.07440511255806115
1106	12-13	-0.05683103482328988
1106	14-15	-0.02033823735374085
1106	16-17	0.012161126541151646
1106	18-19	0.013560364883865361
1106	20-21	0.028257732767613675
1106	22-23	0.00885057798216593
1106	24-25	-0.03260825281881807
1106	26-27	-0.10274225725920871
1106	28-29	-0.06837044236987566
1106	30-31	-0.04718141820654154
1106	32-33	-0.09260606774454772
1106	34-35	-0.0823545009492932
1106	36-37	-0.06654880140453656
1106	38-39	0.03496967808153073
1106	40-41	-0.006236524006766331
1106	42-43	-0.028840573226574406
1106	44-45	-0.03323365205854145
1106	46-47	-0.052271244561609365
1106	48-49	-0.062108415099217495
1106	50-51	-0.070804069596484
1106	52-53	-0.07756156523785052
1106	54-55	-0.06136268006628853
1106	56-57	-0.027641867062165204
1106	58-59	0.0030881203655468425
1106	60-61	-0.021775348939328865
1106	62-63	-0.08538517487603059
1106	64-65	-0.07540585758962237
1106	66-67	-0.014066545386484108
1106	68-69	0.024501193859421733
1106	70-71	-0.06751933427523937
1106	72-73	-0.09018343162440345
1106	74-75	-0.029338215444731475
1106	76-77	-0.10270058418495864
1106	78-79	-0.18142427241458847
1106	80-81	-0.09661745250102882
1106	82-83	-0.16446242193642746
1106	84-85	-0.161598933071204
1106	86-87	-0.13096651540184467
1106	88-89	-0.11743420053121412
1106	90-91	-0.11804912678044133
1106	92-93	-0.031408071432689866
1106	94-95	-0.13487052209609374
1106	96-97	-0.22085853431642022
1106	98-99	-0.24017175924504386
1106	100	-0.17863232142491725
1107	1	-0.09773809318280513
1107	2	-0.05352787752554633
1107	3	0.029739478291805455
1107	4	0.03984976647902272
1107	5	0.0011178946567795833
1107	6	0.03271786193274551
1107	7	0.06138644641821145
1107	8	0.08047937938372485
1107	9	0.11770546902778278
1107	10-11	0.08587569764991798
1107	12-13	0.06611133539285419
1107	14-15	0.08889797617045758
1107	16-17	0.06942989746256245
1107	18-19	0.1152657899609082
1107	20-21	0.10432438121021193
1107	22-23	0.07859610816664997
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2216	22-23	-0.3107137629959169
2216	24-25	-0.41809928775133187
2216	26-27	-0.5106479229426739
2216	28-29	-0.3920124385560726
2216	30-31	-0.5574000348645427
2216	32-33	-0.5550018116381992
2216	34-35	-0.6569386443978544
2216	36-37	-0.7603419718646123
2216	38-39	-0.667453496835158
2216	40-41	-0.6311967929354267
2216	42-43	-0.644149905660484
2216	44-45	-0.528392837947635
2216	46-47	-0.45280395857917455
2216	48-49	-0.532233966218925
2216	50-51	-0.4577784445737052
2216	52-53	-0.4887483493895246
2216	54-55	-0.5097103953778586
2216	56-57	-0.5842660246746902
2216	58-59	-0.5328932347606283
2216	60-61	-0.48057791844885145
2216	62-63	-0.5099207347688051
2216	64-65	-0.5455399575465449
2216	66-67	-0.5025585024722545
2216	68-69	-0.4986113583562286
2216	70-71	-0.48317202164660245
2216	72-73	-0.47095392010403003
2216	74-75	-0.49915343910193144
2216	76-77	-0.4476487118961785
2216	78-79	-0.48869720735653743
2216	80-81	-0.41069234218365835
2216	82-83	-0.5266586228892294
2216	84-85	-0.3643460528605047
2216	86-87	-0.3469046545208627
2216	88-89	-0.4267939393385447
2216	90-91	-0.44027472545597846
2216	92-93	-0.6643641191977991
2216	94-95	-0.5510727642271895
2216	96-97	-0.4450276387252856
2216	98-99	-0.39769620751803814
2216	100	-0.4140784632832961
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41035.0
3	8048.0
4	4566.0
5	3727.0
6	3169.0
7	3679.0
8	4200.0
9	4782.0
10	5301.0
11	5792.0
12	6374.0
13	7196.0
14	7941.0
15	8158.0
16	8186.0
17	8406.0
18	8250.0
19	8189.0
20	8189.0
21	8395.0
22	9860.0
23	13341.0
24	20097.0
25	26985.0
26	28997.0
27	26378.0
28	28244.0
29	35199.0
30	43146.0
31	52569.0
32	68662.0
33	92601.0
34	134411.0
35	206623.0
36	379028.0
37	793510.0
38	1259853.0
39	507358.0
40	822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.105310364406115	19.58996597449939	13.23499915656256	24.069724504531933
2	15.342896757542078	20.080311373039617	40.52312686281482	24.053665006603488
3	17.965931987398445	25.55646066231187	30.0044689646093	26.47313838568039
4	12.030405847650316	16.65997570680068	39.31982642424218	31.98979202130683
5	12.699359487796007	38.2773045913063	36.094748067428945	12.92858785346875
6	30.822606470198632	38.323149833132916	17.94183122954351	12.912412467124934
7	27.393671201249465	32.4173198290426	22.87453006261716	17.314478907090784
8	25.006077515917944	36.76273300681531	20.44911106200507	17.78207841526168
9	25.160515295255536	16.814105796042682	21.01713706565693	37.00824184304486
10-11	23.82754050950713	26.997390068316644	29.143358192704305	20.03171122947192
12-13	24.468378222351657	24.349869876215184	29.022620648776922	22.159131252656245
14-15	21.816030424573867	26.28215072286147	25.903682957129455	25.998135895435208
16-17	21.575244877038113	29.599401071631192	26.54844895349561	22.276905097835083
18-19	21.548621864214876	28.710243634409093	28.306469277302682	21.434665224073353
20-21	22.733440774626775	27.94997665074596	27.780725103978455	21.53585747064881
22-23	22.40381235570021	27.723249882554533	27.741917733404065	22.13102002834119
24-25	22.17758068154142	27.924266892180633	27.695239980233254	22.20291244604469
26-27	21.932168590562558	27.84841395245558	27.843342204601257	22.376075252380602
28-29	22.054351064318116	27.834318911883223	27.97461132854514	22.136718695253524
30-31	22.275188977771194	28.23645007777202	27.48765009176275	22.000710852694038
32-33	21.89681276037722	27.945228167533315	28.0005344170495	22.157424655039968
34-35	22.06362221482522	27.740681383844862	28.066413654923	22.12928274640692
36-37	22.39526539280861	27.990903095338798	27.48363109968927	22.13020041216332
38-39	22.18485521208711	28.185331511804108	27.662764883851793	21.96704839225699
40-41	21.99413467108956	27.89076419667434	27.927195849389257	22.187905282846838
42-43	21.97801448872634	27.804160831369018	27.93759845959715	22.28022622030749
44-45	21.95788501026958	27.99428714784429	27.89190637701558	22.155921464870552
46-47	22.08341809893747	28.12976685965362	27.713440219866758	22.07337482154215
48-49	22.04632390885001	28.00285575076682	27.728133761473057	22.22268657891011
50-51	22.07837012080156	27.879421918744953	27.73055929630168	22.311648664151807
52-53	22.046228742687912	27.87925999755422	27.84753693640613	22.226974323351733
54-55	22.100268073970362	28.23382468870105	27.632037263169074	22.033869974159515
56-57	21.929344297075406	28.03111679728435	27.73023536899764	22.3093035366426
58-59	21.874518635972176	27.579989040008083	28.102761624804657	22.442730699215083
60-61	22.112862326620277	28.031077010824184	27.75088710090103	22.10517356165451
62-63	21.960329736881697	28.047295203488186	27.716156772888645	22.276218286741475
64-65	22.011286606251993	27.81159673719617	27.782705185307094	22.39441147124474
66-67	21.878593411171423	27.782141642520404	27.73245830309023	22.606806643217947
68-69	22.236045944532616	27.961754292079338	27.664452828112	22.13774693527605
70-71	22.174727278711593	27.788797598941823	27.723728654917828	22.312746467428756
72-73	22.003354077322747	27.919016672160797	27.7791991664691	22.298430084047354
74-75	22.028613621735822	27.96948353487616	27.699459772476505	22.302443070911515
76-77	22.012067757683702	27.89789267427481	27.62275355873888	22.467286009302608
78-79	22.14469526502045	27.971888714462523	27.669992966522006	22.213423053995022
80-81	22.132324735466742	27.764821215726887	27.763882789834764	22.33897125897161
82-83	21.94006405060489	27.84959433888403	27.875820350279234	22.334521260231842
84-85	21.998769034270364	27.77140184835109	27.767301200191586	22.46252791718696
86-87	22.124741288320855	27.83050597776465	27.691292389284385	22.353460344630115
88-89	21.88891522676742	27.777453553351172	27.814642237912974	22.518988981968437
90-91	21.903525454186706	27.67077593670083	27.90389951109092	22.521799098021546
92-93	22.10556557854881	27.76692352238334	27.745597792099197	22.381913106968653
94-95	21.890309937765643	27.580354176026244	27.943168328413332	22.586167557794784
96-97	21.964655862248488	27.558207919169327	27.905826094501702	22.571310124080483
98-99	21.85725434502011	27.712398959635014	27.826755294540188	22.603591400804685
100	21.97872014381978	27.535486996157676	27.77167272772151	22.714120132301037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	435.0
1	415.0
2	397.0
3	411.5
4	418.5
5	418.5
6	413.0
7	383.5
8	349.5
9	327.0
10	320.0
11	290.5
12	278.0
13	308.0
14	359.0
15	426.5
16	488.5
17	617.5
18	862.5
19	1260.5
20	1858.5
21	2811.5
22	4467.0
23	6848.0
24	9877.0
25	14073.5
26	19711.5
27	27005.0
28	36648.5
29	47407.5
30	57402.0
31	67940.0
32	79234.0
33	90522.5
34	101559.5
35	111142.0
36	119391.5
37	127958.5
38	134470.0
39	138105.0
40	141491.0
41	143923.0
42	146042.0
43	147560.0
44	145658.0
45	142485.5
46	140901.5
47	137052.5
48	132121.0
49	129219.5
50	125821.0
51	120908.5
52	113380.0
53	106890.0
54	102062.0
55	96069.5
56	87735.5
57	79317.5
58	72315.5
59	64867.5
60	57505.0
61	49513.0
62	41810.5
63	34934.0
64	28628.0
65	22784.5
66	17943.0
67	13863.5
68	10970.5
69	8899.5
70	6387.5
71	4393.0
72	3228.5
73	2279.0
74	1561.5
75	1103.0
76	712.0
77	476.5
78	335.5
79	203.5
80	143.5
81	92.5
82	57.5
83	43.0
84	29.0
85	24.5
86	24.5
87	19.0
88	13.5
89	9.5
90	6.0
91	6.0
92	4.5
93	4.0
94	3.5
95	2.5
96	3.0
97	1.5
98	1.0
99	1.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06216484245362758
2	0.04255683303150362
3	0.05712792260207279
4	0.07545100349063685
5	0.08842878167959177
6	0.08845448025026295
7	0.09033047590926041
8	0.10248589983673698
9	0.09732048713182621
10-11	0.10280713197012696
12-13	0.10790829824835972
14-15	0.10654627400278624
16-17	0.07681302773621033
18-19	0.09825848496132494
20-21	0.09155115801614229
22-23	0.05725641545542879
24-25	0.022987371465386465
26-27	0.18000563826640525
28-29	0.06690622874246357
30-31	0.07655604202949835
32-33	0.0749884292185553
34-35	0.020610253678300668
36-37	0.052219495603874004
38-39	0.0760420706160744
40-41	0.08132312688900556
42-43	0.07538675706395886
44-45	0.07302248856220865
46-47	0.07951137765668612
48-49	0.07638900132013557
50-51	0.071506272892608
52-53	0.07525826421060287
54-55	0.06685483160112118
56-57	0.06918055224686458
58-59	0.06625091519034802
60-61	0.06362966098188584
62-63	0.06969452366028854
64-65	0.06648220232638881
66-67	0.06783137728662669
68-69	0.06736880301454513
70-71	0.059492191103823
72-73	0.05831005685294789
74-75	0.06039164107731492
76-77	0.05508488623371257
78-79	0.051666976334443254
80-81	0.045614962941376166
82-83	0.051358593486388875
84-85	0.04209425875942206
86-87	0.04010261953240423
88-89	0.042222751612778046
90-91	0.03814952816139319
92-93	0.04110486378858094
94-95	0.03943445669495308
96-97	0.04251828517549682
98-99	0.04292946230623599
100	0.05024070566219177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3891267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.41955791786681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84829029962462	38.55532637342263
2	15.101852831752819	16.43672309689347
3	5.905888564734694	9.64187534415141
4	2.8856234126518183	6.281374017358325
5	1.5753339603648129	4.286448884802773
6	0.9334472779327335	3.0478672922841303
7	0.6173103557752189	2.3515629661585944
8	0.4171011808418942	1.8158769494748879
9	0.2973175906011914	1.4561902657549932
>10	1.3406467378480775	12.349223898875056
>50	0.05783281580543837	2.108153101449459
>100	0.01930767158812401	1.653186901880982
>500	4.7300478763443235E-5	0.01619090749330708
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5698570671197838E-5	0.0
3	0.0	0.0	0.0	2.5698570671197838E-5	0.0
4	0.0	0.0	0.0	2.5698570671197838E-5	0.0
5	0.0	0.0	0.0	2.5698570671197838E-5	0.0
6	0.0	0.0	0.0	2.5698570671197838E-5	0.0
7	0.0	0.0	0.0	2.5698570671197838E-5	0.0
8	0.0	0.0	0.0	2.5698570671197838E-5	0.0
9	0.0	0.0	0.0	2.5698570671197838E-5	0.0
10-11	0.0	0.0	0.0	5.1397141342395676E-5	0.0
12-13	0.0	0.0	0.0	5.1397141342395676E-5	0.0
14-15	0.0	0.0	0.0	6.42464266779946E-5	0.0
16-17	0.0	0.0	0.0	7.709571201359352E-5	0.0
18-19	0.0	0.0	0.0	7.709571201359352E-5	0.0
20-21	0.0	0.0	0.0	7.709571201359352E-5	0.0
22-23	0.0	0.0	0.0	7.709571201359352E-5	0.0
24-25	0.0	0.0	0.0	1.284928533559892E-4	0.0
26-27	0.0	0.0	0.0	2.4413642137637948E-4	0.0
28-29	0.0	2.5698570671197838E-5	0.0	9.251485441631222E-4	0.0
30-31	0.0	2.5698570671197838E-5	0.0	0.0036363477499744947	0.0
32-33	0.0	2.5698570671197838E-5	0.0	0.010292277553814734	0.0
34-35	0.0	2.5698570671197838E-5	0.0	0.019312475859405175	0.0
36-37	0.0	2.5698570671197838E-5	0.0	0.03638917607041614	0.0
38-39	0.0	2.5698570671197838E-5	0.0	0.06585258734494447	0.0
40-41	0.0	2.5698570671197838E-5	0.0	0.10364233551694088	0.0
42-43	0.0	2.5698570671197838E-5	0.0	0.1404555380034318	0.0
44-45	0.0	2.5698570671197838E-5	0.0	0.18347494530701697	0.0
46-47	0.0	2.5698570671197838E-5	0.0	0.23936933651687226	0.0
48-49	0.0	2.5698570671197838E-5	0.0	0.30251072465600537	0.0
50-51	0.0	2.5698570671197838E-5	0.0	0.3707018819320288	0.0
52-53	0.0	2.5698570671197838E-5	0.0	0.44076903486704977	0.0
54-55	0.0	2.5698570671197838E-5	0.0	0.515885956938961	0.0
56-57	0.0	2.5698570671197838E-5	0.0	0.6008839794339478	0.0
58-59	0.0	5.1397141342395676E-5	0.0	0.7018793621717554	0.0
60-61	0.0	5.1397141342395676E-5	0.0	0.8128072424739808	0.0
62-63	0.0	5.1397141342395676E-5	0.0	0.9244546827549999	0.0
64-65	0.0	5.1397141342395676E-5	0.0	1.0442614192241242	0.0
66-67	0.0	5.1397141342395676E-5	0.0	1.1822370451577853	0.0
68-69	0.0	5.1397141342395676E-5	0.0	1.3401162140762892	0.0
70-71	0.0	5.1397141342395676E-5	0.0	1.5085189476846486	0.0
72-73	0.0	5.1397141342395676E-5	0.0	1.6887301745164236	0.0
74-75	0.0	5.1397141342395676E-5	0.0	1.8760213575681135	0.0
76-77	0.0	5.1397141342395676E-5	0.0	2.074144488157713	0.0
78-79	0.0	5.1397141342395676E-5	0.0	2.3073590169988334	0.0
80-81	0.0	5.1397141342395676E-5	0.0	2.5619933044943974	0.0
82-83	0.0	5.1397141342395676E-5	0.0	2.8273438959598503	0.0
84-85	0.0	5.1397141342395676E-5	0.0	3.0964336294579633	0.0
86-87	0.0	5.1397141342395676E-5	0.0	3.383396718857894	0.0
88	0.0	5.1397141342395676E-5	0.0	3.6146838548986744	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4930	0.0	33.569763	1
GGTATCA	2985	0.0	33.54963	1
TCAACGC	4825	0.0	31.668085	4
CAACGCA	4945	0.0	30.519688	5
ATCAACG	5020	0.0	30.344296	3
GTACATG	11450	0.0	28.990232	1
AACGCAG	5290	0.0	28.521196	6
TACATGG	11605	0.0	27.183983	2
TATCAAC	6030	0.0	26.19737	2
ACATGGG	12165	0.0	25.314243	3
ACGCAGA	6505	0.0	23.26628	7
CGCAGAG	6700	0.0	22.33093	8
CATGGGG	8450	0.0	21.587917	4
GAGTACA	8105	0.0	19.31718	1
GCAGAGT	8005	0.0	17.635473	9
TAGACCG	160	0.0012765069	17.630825	5
CATGGGA	4300	0.0	17.165915	4
GTATAGG	1465	0.0	17.00947	1
AGTACAT	8540	0.0	16.791046	2
GTATTAG	1955	0.0	16.35365	1
>>END_MODULE