FastQCFastQC Report
Wed 25 May 2016
SRR1033073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3891267
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60060.15434561545121422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.12990627474290506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53450.049.389311
GTATCAA98950.043.686391
TCAACGC115550.036.9720154
ATCAACG116200.036.7670943
CAACGCA117650.036.311625
AACGCAG121250.035.272266
TATCAAC124200.034.5214162
ACGCAGA143950.029.481137
CGCAGAG146450.028.9136858
GTACATG112850.026.985071
TACATGG114450.025.8531022
GCAGAGT162950.025.5533249
ACATGGG122350.023.2024863
GAGTACT107000.021.17051112-13
AGAGTAC151300.020.59409110-11
CAGAGTA160800.020.38572710-11
CCGCGCA1708.638706E-519.3516149
CATGGGG84200.019.312714
AGTACTT114650.019.20452712-13
GTGGTAT28250.018.8561331
Produced by FastQC (version 0.11.5)