##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3891267 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70862986271567 34.0 31.0 34.0 31.0 34.0 2 32.711288122865895 34.0 31.0 34.0 31.0 34.0 3 32.82218156708342 34.0 31.0 34.0 31.0 34.0 4 36.308277226928915 37.0 37.0 37.0 35.0 37.0 5 36.28815370417913 37.0 37.0 37.0 35.0 37.0 6 36.355102335563195 37.0 37.0 37.0 35.0 37.0 7 36.30963334050323 37.0 37.0 37.0 35.0 37.0 8 36.315404211533156 37.0 37.0 37.0 35.0 37.0 9 38.12018450545799 39.0 39.0 39.0 37.0 39.0 10-11 38.08519217519641 39.0 39.0 39.0 36.0 39.0 12-13 38.05657488936123 39.0 39.0 39.0 35.0 39.0 14-15 39.56142549457542 41.0 40.0 41.0 37.0 41.0 16-17 39.55291271454773 41.0 40.0 41.0 37.0 41.0 18-19 39.570461240516266 41.0 40.0 41.0 37.0 41.0 20-21 39.560854086856544 41.0 40.0 41.0 37.0 41.0 22-23 39.4903476168559 41.0 39.0 41.0 36.5 41.0 24-25 39.43440581178315 41.0 39.0 41.0 36.0 41.0 26-27 39.32926049536051 41.0 39.0 41.0 36.0 41.0 28-29 39.281664044127524 41.0 39.0 41.0 36.0 41.0 30-31 39.28131929780197 41.0 39.0 41.0 36.0 41.0 32-33 39.246238949935844 41.0 39.0 41.0 35.5 41.0 34-35 39.16804436704035 41.0 39.0 41.0 35.0 41.0 36-37 39.08461729817049 41.0 39.0 41.0 35.0 41.0 38-39 38.99921902043731 41.0 39.0 41.0 35.0 41.0 40-41 38.90146731128961 41.0 39.0 41.0 35.0 41.0 42-43 38.786671410622816 41.0 39.0 41.0 35.0 41.0 44-45 38.6271322425318 41.0 38.5 41.0 35.0 41.0 46-47 38.43668373308745 41.0 38.0 41.0 35.0 41.0 48-49 38.23123753779939 40.0 38.0 41.0 34.0 41.0 50-51 38.091813925901256 40.0 38.0 41.0 34.0 41.0 52-53 37.956975838460835 40.0 38.0 41.0 34.0 41.0 54-55 37.80425501514031 40.0 37.5 41.0 33.5 41.0 56-57 37.65188690470225 40.0 37.0 41.0 33.0 41.0 58-59 37.467715399637186 40.0 37.0 41.0 33.0 41.0 60-61 37.2710743056182 40.0 36.0 41.0 33.0 41.0 62-63 37.036448282782956 39.0 36.0 41.0 33.0 41.0 64-65 36.76310196653172 39.0 35.0 41.0 32.0 41.0 66-67 36.4781550584938 39.0 35.0 41.0 32.0 41.0 68-69 36.16785779541728 37.5 35.0 40.0 31.5 41.0 70-71 35.83757102763701 37.0 35.0 40.0 31.0 41.0 72-73 35.4860918050599 37.0 35.0 39.0 31.0 41.0 74-75 35.13697518057743 36.0 35.0 39.0 31.0 41.0 76-77 34.78148556755422 36.0 35.0 39.0 31.0 40.5 78-79 34.422218778613754 35.0 34.5 37.0 30.5 39.0 80-81 34.09339605840463 35.0 34.0 37.0 30.0 39.0 82-83 33.80004982952853 35.0 34.0 36.5 30.0 39.0 84-85 33.580456828071675 35.0 34.0 36.0 30.0 37.0 86-87 33.37505509131088 35.0 34.0 36.0 30.0 37.0 88-89 33.1705904529296 35.0 34.0 35.5 30.0 37.0 90-91 32.99474978201188 35.0 34.0 35.0 30.0 36.0 92-93 32.82774993337645 35.0 34.0 35.0 29.5 36.0 94-95 32.6762952786329 35.0 34.0 35.0 29.0 36.0 96-97 32.53644134416888 35.0 34.0 35.0 29.0 36.0 98-99 32.41125448343689 35.0 34.0 35.0 29.0 35.5 100 32.3121284147297 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09887334542681003 1101 2 0.05104708347602127 1101 3 0.1273068699234301 1101 4 0.116119657593174 1101 5 0.06111856947909189 1101 6 0.09139412868574937 1101 7 0.144872276770883 1101 8 0.1511776217929608 1101 9 0.1855854715765446 1101 10-11 0.19338750430856066 1101 12-13 0.20876657447726643 1101 14-15 0.3005269489394067 1101 16-17 0.3261975227165408 1101 18-19 0.31040054442474485 1101 20-21 0.29399123940861216 1101 22-23 0.308065801987226 1101 24-25 0.3252458505606981 1101 26-27 0.36289726323233396 1101 28-29 0.31824156120248404 1101 30-31 0.31056432151985547 1101 32-33 0.32984683660356495 1101 34-35 0.2888828564635162 1101 36-37 0.2617828554232844 1101 38-39 0.2756520234178339 1101 40-41 0.2947941833674932 1101 42-43 0.2744106476972661 1101 44-45 0.20788077684125028 1101 46-47 0.19820073504835278 1101 48-49 0.22465686617346137 1101 50-51 0.23502381730144606 1101 52-53 0.24327153738274632 1101 54-55 0.2203468060281608 1101 56-57 0.21762408783707343 1101 58-59 0.2072864626988249 1101 60-61 0.19369795825989655 1101 62-63 0.18552075841443383 1101 64-65 0.14170183109058598 1101 66-67 0.23739471758224084 1101 68-69 0.21695858272570234 1101 70-71 0.19872209040885025 1101 72-73 0.17302262141820535 1101 74-75 0.12976061098692782 1101 76-77 0.15777019114386093 1101 78-79 0.13956860516397995 1101 80-81 0.10457102274911989 1101 82-83 0.17856298441692786 1101 84-85 0.19404407881592078 1101 86-87 0.21753235207354749 1101 88-89 0.22366988562671253 1101 90-91 0.23369733787489366 1101 92-93 0.2606060013875009 1101 94-95 0.23132989661899472 1101 96-97 0.18307654115881178 1101 98-99 0.2523679372361869 1101 100 0.2155754253225055 1102 1 0.12254471635829844 1102 2 0.08661215840322001 1102 3 0.11685217690581595 1102 4 0.1109299805377546 1102 5 0.11876078349648367 1102 6 0.10092582544854167 1102 7 0.11004487953135111 1102 8 0.09691922259329289 1102 9 0.19687078550600745 1102 10-11 0.19392216446478727 1102 12-13 0.21896979076713308 1102 14-15 0.26160910336712817 1102 16-17 0.2335481770576635 1102 18-19 0.22774448830293892 1102 20-21 0.24779022271195572 1102 22-23 0.21407237615866848 1102 24-25 0.22724422452751725 1102 26-27 0.2641759424163581 1102 28-29 0.2180025778612773 1102 30-31 0.21810408030284378 1102 32-33 0.2463774799467231 1102 34-35 0.25000675015994034 1102 36-37 0.2517728133906161 1102 38-39 0.24633411500003177 1102 40-41 0.2647345274467128 1102 42-43 0.23606106191927978 1102 44-45 0.26483415095476204 1102 46-47 0.31262084788635747 1102 48-49 0.3304554065294667 1102 50-51 0.29448987646141944 1102 52-53 0.3054899274584528 1102 54-55 0.24622458474188846 1102 56-57 0.26961446414355095 1102 58-59 0.27012734522407555 1102 60-61 0.21010849472780535 1102 62-63 0.1939665716629193 1102 64-65 0.18899043494517542 1102 66-67 0.22740351027625394 1102 68-69 0.24838380365515178 1102 70-71 0.21787643957438974 1102 72-73 0.21716790814615905 1102 74-75 0.2083778013189388 1102 76-77 0.2337421908144961 1102 78-79 0.2320382164208965 1102 80-81 0.18202102790737484 1102 82-83 0.24156505055805155 1102 84-85 0.2794807652669391 1102 86-87 0.25931884956209217 1102 88-89 0.3128219348733694 1102 90-91 0.23148591380864758 1102 92-93 0.29363141691091244 1102 94-95 0.2989403187534876 1102 96-97 0.19872166840679029 1102 98-99 0.171137830824712 1102 100 0.2024020956740884 1103 1 0.0948039829875782 1103 2 0.06651989913658696 1103 3 0.10024713701724863 1103 4 0.10386798592858781 1103 5 0.07722168184847789 1103 6 0.11460335217659434 1103 7 0.12410265915833918 1103 8 0.14171218194494628 1103 9 0.20070551478838183 1103 10-11 0.19801359158901022 1103 12-13 0.1795675765447342 1103 14-15 0.2087724927193264 1103 16-17 0.2380760266931503 1103 18-19 0.28518411275344846 1103 20-21 0.282484319927903 1103 22-23 0.24098241432867695 1103 24-25 0.2271754823288461 1103 26-27 0.2063463868889457 1103 28-29 0.2662851082977937 1103 30-31 0.2916588660261752 1103 32-33 0.2403798135634574 1103 34-35 0.24886255435320948 1103 36-37 0.23159724943323567 1103 38-39 0.2428126149728982 1103 40-41 0.1847201458551453 1103 42-43 0.1886705519607048 1103 44-45 0.200695599244348 1103 46-47 0.13611231842670435 1103 48-49 0.14291945255055793 1103 50-51 0.17164286403355078 1103 52-53 0.1546680059330967 1103 54-55 0.23238940320820944 1103 56-57 0.20270334057040884 1103 58-59 0.22752617660670182 1103 60-61 0.19590427612762085 1103 62-63 0.16037994744757356 1103 64-65 0.1337878263596366 1103 66-67 0.17515952974115834 1103 68-69 0.13369115364339734 1103 70-71 0.10465496306432698 1103 72-73 0.1291907377921362 1103 74-75 0.16216920492609432 1103 76-77 0.16840300233423022 1103 78-79 0.15304510873081512 1103 80-81 0.11907343478883803 1103 82-83 0.15022412543277852 1103 84-85 0.20725451298307007 1103 86-87 0.21449197136241338 1103 88-89 0.1693529864481107 1103 90-91 0.1531028026814596 1103 92-93 0.22124244460678 1103 94-95 0.2159440633943106 1103 96-97 0.19294886137338096 1103 98-99 0.1772930615633257 1103 100 0.21544429976606239 1104 1 0.08074706831813216 1104 2 0.0953963214851612 1104 3 0.11714584714485454 1104 4 0.07452259444161058 1104 5 0.09950725006248717 1104 6 0.10681948698103838 1104 7 0.09447428334741659 1104 8 0.12118842604656521 1104 9 0.14205148661430655 1104 10-11 0.18602931601091655 1104 12-13 0.18314809511903007 1104 14-15 0.2223702832860539 1104 16-17 0.2110266011596167 1104 18-19 0.21710458559152102 1104 20-21 0.2161772446289234 1104 22-23 0.23562864937976258 1104 24-25 0.1987651274239468 1104 26-27 0.1968009857296522 1104 28-29 0.2592677050382193 1104 30-31 0.2162227827613563 1104 32-33 0.20550569184427303 1104 34-35 0.16428644359137934 1104 36-37 0.15228042582172918 1104 38-39 0.19509669779555594 1104 40-41 0.20593398693900156 1104 42-43 0.16775831440863698 1104 44-45 0.19838084136313938 1104 46-47 0.2018968814107538 1104 48-49 0.13334420538834735 1104 50-51 0.18081028491055662 1104 52-53 0.22150436416803387 1104 54-55 0.17833922461581864 1104 56-57 0.19541981045150436 1104 58-59 0.13845140269707912 1104 60-61 0.22397274921439703 1104 62-63 0.20533441847553746 1104 64-65 0.18654523086279085 1104 66-67 0.19054759686702738 1104 68-69 0.21505374112603448 1104 70-71 0.18665056906944955 1104 72-73 0.19373776251871533 1104 74-75 0.1641240835730713 1104 76-77 0.21972980224856542 1104 78-79 0.167223825490872 1104 80-81 0.18697246962990022 1104 82-83 0.19738837517196828 1104 84-85 0.2783340470538036 1104 86-87 0.2905488100350979 1104 88-89 0.3343294619944217 1104 90-91 0.28829158555870293 1104 92-93 0.2878192738834997 1104 94-95 0.30268297304797187 1104 96-97 0.3202680063406689 1104 98-99 0.2504673428757229 1104 100 0.271750856905264 1105 1 0.06621289057421365 1105 2 0.03856955368167547 1105 3 0.06617163856560637 1105 4 0.04091934601098757 1105 5 0.045096582358802095 1105 6 0.07531874147385054 1105 7 0.09899781877656721 1105 8 0.10474904161601017 1105 9 0.1257912263987393 1105 10-11 0.14984895038724488 1105 12-13 0.1622092531882302 1105 14-15 0.18922961016061635 1105 16-17 0.21349578952313664 1105 18-19 0.17988935577579923 1105 20-21 0.18951658468080268 1105 22-23 0.2171117810058263 1105 24-25 0.19689918528444394 1105 26-27 0.2089242229299586 1105 28-29 0.23580434742750356 1105 30-31 0.1780741275753357 1105 32-33 0.15421717218779207 1105 34-35 0.08939113715203462 1105 36-37 0.15103398737575446 1105 38-39 0.12778519260828602 1105 40-41 0.11252411607847534 1105 42-43 0.1367242329115257 1105 44-45 0.1506006576673613 1105 46-47 0.08583444961261932 1105 48-49 0.050168654961048276 1105 50-51 0.06960805769564615 1105 52-53 0.0665027293350775 1105 54-55 0.09684409945536032 1105 56-57 0.0620544337863862 1105 58-59 0.06524129168030157 1105 60-61 0.13638814709536007 1105 62-63 0.0936342129629466 1105 64-65 0.12866863939930084 1105 66-67 0.05841493228140138 1105 68-69 0.10211540437745015 1105 70-71 0.0971682562227798 1105 72-73 0.06937272865580724 1105 74-75 0.0574067762409598 1105 76-77 0.03743275225564702 1105 78-79 0.11174649255696778 1105 80-81 0.080511971906013 1105 82-83 0.10248911242387493 1105 84-85 0.019860206133500924 1105 86-87 0.02496159607732551 1105 88-89 0.054144208616079936 1105 90-91 0.04340217464243068 1105 92-93 0.02198706213167867 1105 94-95 0.07811056396617033 1105 96-97 0.022273100737180584 1105 98-99 -0.009847421204057127 1105 100 -0.028453020199428636 1106 1 -0.027127965729690118 1106 2 -0.002730401244996017 1106 3 0.0027053806762253885 1106 4 6.324055974715748E-5 1106 5 -0.026309353180636208 1106 6 -0.022739558157852002 1106 7 -0.01785899762764842 1106 8 0.007903343464413126 1106 9 -0.017286879946034617 1106 10-11 0.05767914475325142 1106 12-13 0.031450341903806134 1106 14-15 0.04763480572353984 1106 16-17 0.037920422431028555 1106 18-19 0.049472835570576024 1106 20-21 0.05916229766665282 1106 22-23 0.044608383141806485 1106 24-25 0.04110823646487205 1106 26-27 0.03939139791393842 1106 28-29 0.024441887679074625 1106 30-31 0.019848456318904084 1106 32-33 0.03913649787892126 1106 34-35 -0.0223797050444432 1106 36-37 0.03687412863362738 1106 38-39 0.027197657950566168 1106 40-41 0.10453470148846122 1106 42-43 0.0807035081116041 1106 44-45 -0.011994763458154978 1106 46-47 0.03716886938594399 1106 48-49 0.0012294595484618753 1106 50-51 0.00158107125063367 1106 52-53 0.03991867601509114 1106 54-55 0.06083567121460476 1106 56-57 0.0017376963014257285 1106 58-59 -0.024799965173414762 1106 60-61 0.03417929362236549 1106 62-63 0.036746254536573986 1106 64-65 0.05321472470522082 1106 66-67 0.03902837852047725 1106 68-69 0.027212285982933793 1106 70-71 0.01262907083199849 1106 72-73 0.025848844133818716 1106 74-75 0.03604485355226217 1106 76-77 -0.02673438097907166 1106 78-79 0.06350736627997122 1106 80-81 0.031824996112895576 1106 82-83 0.059698754742100846 1106 84-85 0.07317057778627145 1106 86-87 0.04150855506654949 1106 88-89 0.02430446326921043 1106 90-91 0.03331221155691111 1106 92-93 0.0891919937043184 1106 94-95 0.05782979956025969 1106 96-97 0.05847852322953884 1106 98-99 0.07724037925481042 1106 100 0.0169062008183829 1107 1 0.03133270750563355 1107 2 0.009690338000737597 1107 3 0.0379199435769948 1107 4 0.022083649539716532 1107 5 0.06048305046938651 1107 6 0.06509632593183312 1107 7 0.055218565782439555 1107 8 0.03974275227302826 1107 9 0.07269697251404494 1107 10-11 0.07489287593340777 1107 12-13 0.07437966571993115 1107 14-15 0.1261810746953813 1107 16-17 0.1116415777626969 1107 18-19 0.14698575461188312 1107 20-21 0.11537942412891766 1107 22-23 0.12794532843527406 1107 24-25 0.13093850145290986 1107 26-27 0.14255263094569415 1107 28-29 0.1868179118478963 1107 30-31 0.13915850582908007 1107 32-33 0.14103711724003887 1107 34-35 0.1742148271168702 1107 36-37 0.16094846241698235 1107 38-39 0.17773097029693474 1107 40-41 0.12546424902840414 1107 42-43 0.1560088394716317 1107 44-45 0.12447425892693076 1107 46-47 0.09195076122259849 1107 48-49 0.09851083215347245 1107 50-51 0.12464838065224626 1107 52-53 0.12837052795314463 1107 54-55 0.09885663807913403 1107 56-57 0.09514938304484843 1107 58-59 0.07960411365385056 1107 60-61 0.1005309518445685 1107 62-63 0.10979157749153501 1107 64-65 0.12109222332565395 1107 66-67 0.10426135826171645 1107 68-69 0.10218029490149405 1107 70-71 0.13465496306432811 1107 72-73 0.11284640217168374 1107 74-75 0.09884212034540951 1107 76-77 0.11516214093487775 1107 78-79 0.07084031826948234 1107 80-81 0.04526978543190552 1107 82-83 0.0827794917292124 1107 84-85 0.0772731311709407 1107 86-87 0.13674490016505558 1107 88-89 0.09900031346546001 1107 90-91 0.06816877478139105 1107 92-93 0.1338685215961135 1107 94-95 0.10548698127897893 1107 96-97 0.10671019957764116 1107 98-99 0.11263014201375654 1107 100 0.1345406372819511 1108 1 -0.0628561813510089 1108 2 -0.017164718633658538 1108 3 -0.012990138959430908 1108 4 -0.039374803504372835 1108 5 -0.052781732735240894 1108 6 -0.01754818015700721 1108 7 -0.019110934017504633 1108 8 -0.017871395473690654 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-0.1583875044971137 2214 62-63 -0.10028363476926216 2214 64-65 -0.11616856855496138 2214 66-67 -0.13366617485238663 2214 68-69 -0.19046436618190654 2214 70-71 -0.0884629295511914 2214 72-73 -0.1506205286429534 2214 74-75 -0.1681333146900883 2214 76-77 -0.17051629498699583 2214 78-79 -0.16571135191053799 2214 80-81 -0.1471269340414736 2214 82-83 -0.15367734146890655 2214 84-85 -0.08917029099094975 2214 86-87 -0.08578705501248152 2214 88-89 -0.05607544607740067 2214 90-91 -0.06149896650898512 2214 92-93 -0.09534022580069745 2214 94-95 -0.11237887225593823 2214 96-97 -0.08436635002505 2214 98-99 -0.07605722402214354 2214 100 -0.09674486292395557 2215 1 -0.22109203779340447 2215 2 -0.15691294341667117 2215 3 -0.18098584510187266 2215 4 -0.15043571044402881 2215 5 -0.15830478127137582 2215 6 -0.20736914326628408 2215 7 -0.18907278255699822 2215 8 -0.16183897318807539 2215 9 -0.2624649121080296 2215 10-11 -0.21495565881496503 2215 12-13 -0.2452959620070203 2215 14-15 -0.37448747124827264 2215 16-17 -0.3768665814902903 2215 18-19 -0.39290984478774504 2215 20-21 -0.3405831439799982 2215 22-23 -0.3514415474844057 2215 24-25 -0.39475280841390514 2215 26-27 -0.4585967917633127 2215 28-29 -0.4522829088321956 2215 30-31 -0.44800090902337075 2215 32-33 -0.4101014631192257 2215 34-35 -0.37933518871293614 2215 36-37 -0.3512758305411907 2215 38-39 -0.368352905891669 2215 40-41 -0.4096076561417519 2215 42-43 -0.42469858381883085 2215 44-45 -0.41360683679017285 2215 46-47 -0.36619806702448443 2215 48-49 -0.3279566548886166 2215 50-51 -0.37599918933579346 2215 52-53 -0.36986362589959043 2215 54-55 -0.389399547208761 2215 56-57 -0.34361193499255904 2215 58-59 -0.33481797263856095 2215 60-61 -0.3153381651605258 2215 62-63 -0.35343682062103454 2215 64-65 -0.31230163641793496 2215 66-67 -0.3549664908010257 2215 68-69 -0.2910265571580979 2215 70-71 -0.32484440411328563 2215 72-73 -0.24988146764032138 2215 74-75 -0.28243666745340335 2215 76-77 -0.2764829569687848 2215 78-79 -0.269492867245809 2215 80-81 -0.2412705977093168 2215 82-83 -0.30112471492351744 2215 84-85 -0.2727997346216213 2215 86-87 -0.24597176672076415 2215 88-89 -0.2633140746822633 2215 90-91 -0.24421074024830602 2215 92-93 -0.20321572941539756 2215 94-95 -0.17035128556013746 2215 96-97 -0.24139560361973622 2215 98-99 -0.24284351928537262 2215 100 -0.23095715638876868 2216 1 -0.1955796732081012 2216 2 -0.1717439495169657 2216 3 -0.1585978638644292 2216 4 -0.13860272974262244 2216 5 -0.1835053252301435 2216 6 -0.16722499757275244 2216 7 -0.1462628596466118 2216 8 -0.1529808679076865 2216 9 -0.21430922184159584 2216 10-11 -0.20456620033481698 2216 12-13 -0.2573471777188132 2216 14-15 -0.343326784304125 2216 16-17 -0.32544098970607394 2216 18-19 -0.330052932867126 2216 20-21 -0.35515240325017317 2216 22-23 -0.33051996620385893 2216 24-25 -0.3548634626795959 2216 26-27 -0.37153027885405265 2216 28-29 -0.3827017169031066 2216 30-31 -0.33894558771088157 2216 32-33 -0.36196205106107016 2216 34-35 -0.3699549885341611 2216 36-37 -0.3467536335050312 2216 38-39 -0.39032729484397066 2216 40-41 -0.34797010781641546 2216 42-43 -0.3585294883446082 2216 44-45 -0.3434287678362793 2216 46-47 -0.23936076518162963 2216 48-49 -0.28992433917174054 2216 50-51 -0.24523422995339672 2216 52-53 -0.23266340450093992 2216 54-55 -0.2360305025781173 2216 56-57 -0.23330844873357393 2216 58-59 -0.1443407150979681 2216 60-61 -0.1829408507081638 2216 62-63 -0.23092657445584308 2216 64-65 -0.3277423900295062 2216 66-67 -0.31177311000492125 2216 68-69 -0.26958780950243266 2216 70-71 -0.283369068978395 2216 72-73 -0.2756731058232518 2216 74-75 -0.3827360775889659 2216 76-77 -0.2674786763820407 2216 78-79 -0.3326784975870396 2216 80-81 -0.264477211355711 2216 82-83 -0.26765619297918875 2216 84-85 -0.39478380225485665 2216 86-87 -0.39854667135617916 2216 88-89 -0.559017923192421 2216 90-91 -0.5213796519120848 2216 92-93 -0.5088071813095354 2216 94-95 -0.4591447342478219 2216 96-97 -0.5680546149598484 2216 98-99 -0.6597516773338192 2216 100 -0.6629691823610528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27.0 3 15.0 4 16.0 5 23.0 6 88.0 7 216.0 8 439.0 9 848.0 10 1565.0 11 2467.0 12 3947.0 13 5989.0 14 7297.0 15 9195.0 16 11608.0 17 16584.0 18 19896.0 19 10559.0 20 7362.0 21 6824.0 22 7254.0 23 7855.0 24 9036.0 25 11087.0 26 14309.0 27 18466.0 28 23634.0 29 30711.0 30 39343.0 31 50505.0 32 64777.0 33 84959.0 34 119308.0 35 181447.0 36 333332.0 37 762988.0 38 1381646.0 39 643570.0 40 2075.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83002190024615 19.783265208947064 13.458572848528355 23.92814004227843 2 15.592347846875532 20.333042140220435 40.03072340664513 24.04388660625891 3 18.008940813051172 25.70600684374394 30.278516991785192 26.006535351419696 4 12.201428317739405 16.919209765953568 38.946805199798206 31.93255671650882 5 12.423347332248165 38.149013145890756 36.063515771522496 13.36412375033858 6 30.657121512600423 38.478060699634746 17.696971597110345 13.167846190654489 7 27.01878334228929 32.69629146496501 22.817555310391192 17.467369882354514 8 25.220115361239497 36.20829269734315 20.33888809651385 18.232703844903508 9 25.406249429812966 16.068031312166447 21.09369518976724 37.43202406825335 10-11 23.971215888800597 26.58888776956675 29.075162081843235 20.36473425978941 12-13 24.923193396906456 23.92033237503363 29.252169023611074 21.904305204448836 14-15 22.018064877568342 25.692511601341728 26.646060619299313 25.643362901790617 16-17 21.20609216317125 29.224789955648063 27.212499082880964 22.356618798299728 18-19 21.053107681474696 27.864578046357913 29.782897247539026 21.299417024628365 20-21 22.187981727799933 27.13114097942148 29.465766479885147 21.215110812893435 22-23 21.857274782811377 26.92648050676638 29.44500888593812 21.771235824484123 24-25 21.64786882077782 27.134764210559858 29.42951655853451 21.78785041012781 26-27 21.384290910174922 27.148781770854345 29.545086004209942 21.921841314760787 28-29 21.46619059048831 27.114819541464012 29.672645373445167 21.746344494602514 30-31 21.726721924639644 27.490029909308376 29.145134992785763 21.638113173266216 32-33 21.47940503697125 27.238801655090743 29.552019946202613 21.729773361735393 34-35 21.587608868782326 27.087102992418664 29.612835613696003 21.712452525103007 36-37 21.908237085761527 27.32995448526148 28.994014031933556 21.76779439704343 38-39 21.733563900909395 27.581980367833918 29.09485522324734 21.589600508009347 40-41 21.679806355980986 27.29289248943274 29.21166489860364 21.815636255982632 42-43 21.655491643210294 27.266543262130305 29.091218875497365 21.986746219162036 44-45 21.70280271181597 27.401396511727416 28.98459550578256 21.91120527067405 46-47 21.891597261251928 27.642693241044626 28.610450015380597 21.855259482322854 48-49 21.973678495975733 27.597643646658014 28.361058236301957 22.067619621064296 50-51 21.98999708835194 27.627119907217878 28.205440541602517 22.17744246282766 52-53 21.969669518951026 27.56671541685523 28.34705251528615 22.11656254890759 54-55 21.99765526241196 27.936659190952458 28.134692376544706 21.930993170090872 56-57 21.821260273324857 27.689940063223624 28.24960353530097 22.23919612815055 58-59 21.721793955541987 27.294696046300597 28.60841982829757 22.375090169859842 60-61 21.9663030061931 27.712040834000856 28.236754249965372 22.084901909840678 62-63 21.796306961203125 27.716756521719017 28.238321862776317 22.248614654301544 64-65 21.861542782851963 27.473917878161537 28.31946509967062 22.345074239315885 66-67 21.687476301096314 27.494184733942024 28.261338564192407 22.55700040076926 68-69 22.070104853007653 27.64133800466019 28.147369860176287 22.14118728215587 70-71 22.08521313048078 27.471762728150335 28.118904217945495 22.32411992342339 72-73 21.923979242675816 27.636702111119476 28.127662220883607 22.3116564253211 74-75 21.949519956322636 27.679025858073498 28.01021674082216 22.361237444781707 76-77 21.94358546971976 27.620399730987362 27.942505615780156 22.49350918351272 78-79 22.107683692740693 27.683977994827906 27.928371401910994 22.279966910520404 80-81 22.013704019796123 27.55468848578111 28.045801020593036 22.38580647382973 82-83 21.852543430008936 27.602157758114913 28.13725422170345 22.408044590172697 84-85 21.884440209319997 27.57155959742675 27.9741405562764 22.56985963697685 86-87 22.004695412997048 27.57766827553499 27.900122955522328 22.51751335594564 88-89 21.736107979014502 27.56787269411835 27.98601240991287 22.710006916954274 90-91 21.76218486498725 27.468438473317597 28.053699536861604 22.71567712483355 92-93 21.84587246451226 27.606686148895783 27.883847263538332 22.663594123053624 94-95 21.640149011472936 27.37521728269931 28.075880104151786 22.90875360167597 96-97 21.705675274811618 27.374294138667032 28.009667314400886 22.910363272120456 98-99 21.586865649241815 27.470201184088864 27.92552904430491 23.017404122364415 100 21.707582645960816 27.344821108394772 27.834892851094516 23.1127033945499 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32.0 1 32.5 2 112.5 3 193.5 4 141.0 5 122.0 6 275.5 7 627.5 8 1115.5 9 1332.5 10 1114.0 11 969.5 12 1064.5 13 1266.5 14 1531.5 15 1777.5 16 2030.0 17 2365.5 18 2735.5 19 3172.5 20 3848.0 21 4879.0 22 6423.5 23 8796.0 24 12047.0 25 16053.0 26 21292.5 27 28028.5 28 37327.0 29 48044.0 30 58108.5 31 68383.5 32 78771.5 33 89980.0 34 101620.5 35 110816.5 36 118280.0 37 126773.5 38 133374.5 39 136739.0 40 139999.0 41 142569.0 42 144396.0 43 146224.5 44 144590.5 45 141841.5 46 140746.5 47 136850.0 48 131796.5 49 128335.0 50 124938.0 51 120225.5 52 112794.5 53 105915.5 54 101055.5 55 95130.5 56 87044.5 57 78771.0 58 71845.5 59 64613.0 60 56858.5 61 48743.0 62 41148.0 63 34579.5 64 28087.5 65 22132.5 66 17472.5 67 13381.5 68 10496.5 69 8407.0 70 6004.5 71 4078.5 72 2832.5 73 2035.5 74 1394.5 75 909.0 76 559.5 77 345.0 78 232.5 79 140.5 80 80.0 81 33.0 82 18.0 83 10.5 84 7.0 85 4.5 86 2.0 87 2.0 88 1.5 89 0.5 90 0.5 91 1.0 92 1.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04659150862688168 2 0.2450615699205426 3 0.003135225621886136 4 0.0029810341978589493 5 3.340814187255719E-4 6 2.055885653695827E-4 7 0.0 8 3.0838284805437407E-4 9 0.0 10-11 5.910671254375502E-4 12-13 0.0 14-15 3.7262927473236866E-4 16-17 1.413421386915881E-4 18-19 5.653685547663524E-4 20-21 1.2849285335598919E-5 22-23 1.6704070936278596E-4 24-25 0.0010536413975191115 26-27 1.0279428268479135E-4 28-29 3.340814187255719E-4 30-31 8.994499734919244E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.709571201359352E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.2849285335598919E-5 68-69 2.569857067119784E-4 70-71 1.2849285335598919E-5 72-73 3.5977998939676977E-4 74-75 1.6704070936278596E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5419142402718703E-4 84-85 0.0 86-87 5.782178401019513E-4 88-89 0.0026598020644689763 90-91 0.002505610640441789 92-93 7.324092641291384E-4 94-95 5.910671254375502E-4 96-97 2.698349920475773E-4 98-99 1.284928533559892E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3891267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.392503988870885 #Duplication Level Percentage of deduplicated Percentage of total 1 68.96772118249413 36.13391607157158 2 16.197556488112433 16.972610858267842 3 6.241178944562287 9.809729782447098 4 3.0388235820155796 6.368463065689046 5 1.654259467171499 4.333539786620509 6 0.9872600091690439 3.1035014381065102 7 0.6258644675127951 2.2953424627459666 8 0.44099617496059446 1.84839150845598 9 0.3206429851315519 1.511935998975747 >10 1.4364474148737898 12.797080979883809 >50 0.06594683371093261 2.3425593568023726 >100 0.022663871551352806 1.9441465349530396 >500 4.420929807131529E-4 0.1480589393589979 >1k 9.824287663369224E-5 0.10605785625325227 >5k 9.824287663369224E-5 0.28466535986829006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6006 0.15434561545121422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5055 0.12990627474290506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.5698570671197838E-5 0.0 2 0.0 0.0 0.0 2.5698570671197838E-5 0.0 3 0.0 0.0 0.0 2.5698570671197838E-5 0.0 4 0.0 0.0 0.0 2.5698570671197838E-5 0.0 5 0.0 0.0 0.0 2.5698570671197838E-5 0.0 6 0.0 0.0 0.0 2.5698570671197838E-5 0.0 7 0.0 0.0 0.0 5.1397141342395676E-5 0.0 8 0.0 0.0 0.0 7.709571201359352E-5 0.0 9 0.0 0.0 0.0 7.709571201359352E-5 0.0 10-11 0.0 0.0 0.0 1.0279428268479135E-4 0.0 12-13 0.0 0.0 0.0 1.0279428268479135E-4 0.0 14-15 0.0 0.0 0.0 1.6704070936278593E-4 0.0 16-17 0.0 0.0 0.0 2.055885653695827E-4 0.0 18-19 0.0 0.0 0.0 2.055885653695827E-4 0.0 20-21 0.0 0.0 0.0 2.1843785070518163E-4 0.0 22-23 0.0 0.0 0.0 2.3128713604078055E-4 0.0 24-25 0.0 0.0 0.0 2.9553356271877517E-4 0.0 26-27 0.0 0.0 0.0 4.111771307391654E-4 0.0 28-29 0.0 0.0 0.0 0.001092189253525908 0.0 30-31 0.0 0.0 0.0 0.003790539174001681 0.0 32-33 0.0 0.0 0.0 0.010485016833848719 0.0 34-35 0.0 0.0 0.0 0.01964655727813075 0.0 36-37 0.0 0.0 0.0 0.03671040820380611 0.0 38-39 0.0 0.0 0.0 0.06630231233169043 0.0 40-41 0.0 0.0 0.0 0.10416915621570044 0.0 42-43 0.0 0.0 0.0 0.14100805727286253 0.0 44-45 0.0 0.0 0.0 0.18424590242715289 0.0 46-47 0.0 0.0 0.0 0.2403587314877134 0.0 48-49 0.0 0.0 0.0 0.3038085024749009 0.0 50-51 0.0 0.0 0.0 0.37225664545763626 0.0 52-53 0.0 0.0 0.0 0.442555085528698 0.0 54-55 0.0 1.2849285335598919E-5 0.0 0.5179161440219856 0.0 56-57 0.0 2.5698570671197838E-5 0.0 0.6032867957917049 0.0 58-59 0.0 2.5698570671197838E-5 0.0 0.7045648628068956 0.0 60-61 0.0 2.5698570671197838E-5 0.0 0.8163150973705993 0.0 62-63 0.0 2.5698570671197838E-5 0.0 0.9288491383397747 0.0 64-65 0.0 2.5698570671197838E-5 0.0 1.0494011333583637 0.0 66-67 0.0 2.5698570671197838E-5 0.0 1.188276209265517 0.0 68-69 0.0 2.5698570671197838E-5 0.0 1.347273266008218 0.0 70-71 0.0 2.5698570671197838E-5 0.0 1.5167553395847677 0.0 72-73 0.0 2.5698570671197838E-5 0.0 1.6981872485234244 0.0 74-75 0.0 2.5698570671197838E-5 0.0 1.8866348672553181 0.0 76-77 0.0 2.5698570671197838E-5 0.0 2.0860814742344846 0.0 78-79 0.0 2.5698570671197838E-5 0.0 2.320696575177185 0.0 80-81 0.0 2.5698570671197838E-5 0.0 2.5770141190517126 0.0 82-83 0.0 2.5698570671197838E-5 0.0 2.8439580218987803 0.0 84-85 0.0 2.5698570671197838E-5 0.0 3.114127095365083 0.0 86-87 0.0 2.5698570671197838E-5 0.0 3.4021952233038752 0.0 88 0.0 2.5698570671197838E-5 0.0 3.634291864320798 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5345 0.0 49.38931 1 GTATCAA 9895 0.0 43.68639 1 TCAACGC 11555 0.0 36.972015 4 ATCAACG 11620 0.0 36.767094 3 CAACGCA 11765 0.0 36.31162 5 AACGCAG 12125 0.0 35.27226 6 TATCAAC 12420 0.0 34.521416 2 ACGCAGA 14395 0.0 29.48113 7 CGCAGAG 14645 0.0 28.913685 8 GTACATG 11285 0.0 26.98507 1 TACATGG 11445 0.0 25.853102 2 GCAGAGT 16295 0.0 25.553324 9 ACATGGG 12235 0.0 23.202486 3 GAGTACT 10700 0.0 21.170511 12-13 AGAGTAC 15130 0.0 20.594091 10-11 CAGAGTA 16080 0.0 20.385727 10-11 CCGCGCA 170 8.638706E-5 19.351614 9 CATGGGG 8420 0.0 19.31271 4 AGTACTT 11465 0.0 19.204527 12-13 GTGGTAT 2825 0.0 18.856133 1 >>END_MODULE