FastQCFastQC Report
Wed 25 May 2016
SRR1033072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599382
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC44150.033.646774
ATCAACG44900.033.398843
CAACGCA46350.032.456845
GGTATCA35650.030.200891
GTATCAA53500.029.7034381
AACGCAG50500.029.5979336
GTACATG96650.025.2469711
TATCAAC62150.025.1121562
CGCAGAG59250.024.4541238
ACGCAGA60400.024.4353357
TACATGG96900.023.868682
ACATGGG99400.022.6535453
CATGGGG66250.018.165244
ATTCGCG800.001278316317.6274716-17
GCAGAGT78300.017.4852969
CTGTCGC10850.016.044049
GAGTACA73300.015.8429761
GTATTAA37200.015.0399991
GTATTAT36650.015.0091351
GTGTAGG18450.014.7800341
Produced by FastQC (version 0.11.5)