##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033072_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4599382 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74099455100707 33.0 31.0 34.0 30.0 34.0 2 31.808266414922702 33.0 31.0 34.0 30.0 34.0 3 31.86802683490956 34.0 31.0 34.0 30.0 34.0 4 35.26275008251108 37.0 35.0 37.0 33.0 37.0 5 35.30064169490597 37.0 35.0 37.0 33.0 37.0 6 35.366863852578454 37.0 35.0 37.0 33.0 37.0 7 35.24684577188848 37.0 35.0 37.0 33.0 37.0 8 35.250598667386186 37.0 35.0 37.0 33.0 37.0 9 36.89621692653491 39.0 38.0 39.0 34.0 39.0 10-11 36.894637475208626 39.0 37.5 39.0 34.0 39.0 12-13 36.863291633528156 39.0 38.0 39.0 34.0 39.0 14-15 38.273911799454794 41.0 39.0 41.0 34.0 41.0 16-17 38.293495191310484 41.0 39.0 41.0 34.0 41.0 18-19 38.306657503116725 41.0 39.0 41.0 34.0 41.0 20-21 38.324382601836504 41.0 39.0 41.0 34.0 41.0 22-23 38.29133837980842 40.0 39.0 41.0 34.0 41.0 24-25 38.304133142235194 40.0 39.0 41.0 34.0 41.0 26-27 38.21378437363977 40.0 38.0 41.0 34.0 41.0 28-29 38.22235454676302 40.0 38.5 41.0 33.5 41.0 30-31 38.37500853375519 40.5 39.0 41.0 34.0 41.0 32-33 38.36076194584403 41.0 39.0 41.0 34.0 41.0 34-35 38.3245115322015 41.0 39.0 41.0 34.0 41.0 36-37 38.2592691800768 41.0 38.5 41.0 33.5 41.0 38-39 38.18189019742218 40.5 38.0 41.0 33.0 41.0 40-41 38.117902687795876 40.0 38.0 41.0 33.0 41.0 42-43 38.01734276474535 40.0 38.0 41.0 33.0 41.0 44-45 37.91028718640896 40.0 38.0 41.0 33.0 41.0 46-47 37.803600135844334 40.0 38.0 41.0 33.0 41.0 48-49 37.70394555181544 40.0 38.0 41.0 32.5 41.0 50-51 37.60323571732028 40.0 38.0 41.0 32.0 41.0 52-53 37.469073236360884 40.0 37.5 41.0 32.0 41.0 54-55 37.33588762577233 40.0 37.0 41.0 31.5 41.0 56-57 37.18862947239433 40.0 37.0 41.0 31.0 41.0 58-59 37.01784130998469 40.0 36.5 41.0 31.0 41.0 60-61 36.82224253171404 40.0 36.0 41.0 31.0 41.0 62-63 36.60116728725728 39.0 36.0 41.0 31.0 41.0 64-65 36.34927757685706 39.0 35.0 41.0 30.5 41.0 66-67 36.073111017958496 39.0 35.0 41.0 30.0 41.0 68-69 35.772265491320354 38.0 35.0 40.0 30.0 41.0 70-71 35.44219527753946 37.5 35.0 40.0 29.5 41.0 72-73 35.09130280981228 37.0 35.0 39.5 29.0 41.0 74-75 34.731189429362466 36.5 34.0 39.0 29.0 41.0 76-77 34.351673333504365 36.0 34.0 39.0 29.0 40.5 78-79 33.94433426055936 35.5 34.0 38.5 28.5 39.5 80-81 33.56299759402459 35.0 34.0 37.0 28.0 39.0 82-83 33.29958122634737 35.0 34.0 37.0 28.0 39.0 84-85 33.04121336301269 35.0 34.0 36.5 28.0 38.5 86-87 32.824906476565765 35.0 34.0 36.0 28.0 37.0 88-89 32.57761760166909 35.0 34.0 36.0 28.0 37.0 90-91 32.3460315972885 35.0 34.0 35.0 27.0 36.5 92-93 32.11025046408409 35.0 33.5 35.0 27.0 36.0 94-95 31.90188540547404 35.0 33.0 35.0 26.5 36.0 96-97 31.72388410008127 35.0 33.0 35.0 26.0 36.0 98-99 31.545528725380933 35.0 33.0 35.0 25.5 35.5 100 31.408363123567472 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15655416767731722 1101 2 0.19372820346780273 1101 3 0.1978832690395187 1101 4 0.09979818908109195 1101 5 0.1078413761574808 1101 6 0.18447635617204838 1101 7 0.20312719592627104 1101 8 0.23173711838921918 1101 9 0.2686298708319015 1101 10-11 0.22910262454079344 1101 12-13 0.25995375676306764 1101 14-15 0.35136622124494465 1101 16-17 0.3163591761692146 1101 18-19 0.3310181771912326 1101 20-21 0.35065392469628165 1101 22-23 0.3487458412872897 1101 24-25 0.27053299861864843 1101 26-27 0.2511753012173301 1101 28-29 0.25611807966513567 1101 30-31 0.27768203457917906 1101 32-33 0.23763544883959042 1101 34-35 0.24385133903170697 1101 36-37 0.2601320202048214 1101 38-39 0.28010154559359535 1101 40-41 0.23600060072247686 1101 42-43 0.2737274673856689 1101 44-45 0.26266300025009315 1101 46-47 0.2272444994916043 1101 48-49 0.2387457465033762 1101 50-51 0.222269941662951 1101 52-53 0.22985608141479474 1101 54-55 0.2182753696055144 1101 56-57 0.22696978979052318 1101 58-59 0.23884279219527826 1101 60-61 0.2646106025669823 1101 62-63 0.2711848022108967 1101 64-65 0.29516266411857117 1101 66-67 0.26549396768604794 1101 68-69 0.25456754439937157 1101 70-71 0.24078624061803566 1101 72-73 0.20012246777888265 1101 74-75 0.27013237239444265 1101 76-77 0.2530652782913734 1101 78-79 0.28816596297519936 1101 80-81 0.2875007475468436 1101 82-83 0.25669967514233605 1101 84-85 0.2570526454872777 1101 86-87 0.21496976016666025 1101 88-89 0.23611079898238074 1101 90-91 0.24725314753153782 1101 92-93 0.22659233339455653 1101 94-95 0.2341627989349746 1101 96-97 0.2919982048390146 1101 98-99 0.3237134542400568 1101 100 0.29938037149946695 1102 1 0.09405702700209417 1102 2 0.18698545161756286 1102 3 0.17076108015117697 1102 4 0.08113353649447674 1102 5 0.09246201257951725 1102 6 0.20613107166720823 1102 7 0.22466225766599024 1102 8 0.2479661099007302 1102 9 0.3124512222589857 1102 10-11 0.2733760564557244 1102 12-13 0.27791826364764205 1102 14-15 0.3204935393763293 1102 16-17 0.32403945976612647 1102 18-19 0.3223580111629616 1102 20-21 0.34996128186245556 1102 22-23 0.326745353365574 1102 24-25 0.37214047375500314 1102 26-27 0.21241493503762854 1102 28-29 0.24319038938103432 1102 30-31 0.3084462777810728 1102 32-33 0.32400330458626314 1102 34-35 0.288368744875541 1102 36-37 0.31450592049217363 1102 38-39 0.3821137050503083 1102 40-41 0.32207746543750204 1102 42-43 0.34849412426287785 1102 44-45 0.3470698755125312 1102 46-47 0.28620304439319 1102 48-49 0.30801286633630554 1102 50-51 0.3089809294539734 1102 52-53 0.3114290815252758 1102 54-55 0.3097497898703878 1102 56-57 0.3054308119195994 1102 58-59 0.2818870085583427 1102 60-61 0.2595459312414974 1102 62-63 0.2746915304014337 1102 64-65 0.2101543699199766 1102 66-67 0.2972914368823325 1102 68-69 0.30099810321038234 1102 70-71 0.1875788402698504 1102 72-73 0.21004970355126318 1102 74-75 0.20022250249417795 1102 76-77 0.21394497050542327 1102 78-79 0.19393197732455292 1102 80-81 0.27858137964284424 1102 82-83 0.21095570720002854 1102 84-85 0.218744778073777 1102 86-87 0.1586671913989548 1102 88-89 0.2315464147265729 1102 90-91 0.24083337193683718 1102 92-93 0.27908860823356463 1102 94-95 0.29991630205550734 1102 96-97 0.28118440192477223 1102 98-99 0.29006872399065387 1102 100 0.20585897533228703 1103 1 0.030738559029629187 1103 2 0.13537292266988388 1103 3 0.12490595358778123 1103 4 0.03785608089961556 1103 5 0.033121379911172255 1103 6 0.09176914148434179 1103 7 0.10517212312167601 1103 8 0.1162153981125229 1103 9 0.20693065939028088 1103 10-11 0.2486338017478218 1103 12-13 0.2436074966413173 1103 14-15 0.3318575549161693 1103 16-17 0.2828137195189768 1103 18-19 0.2726567873978496 1103 20-21 0.2699163112364502 1103 22-23 0.2844943659777215 1103 24-25 0.23971483625684442 1103 26-27 0.20408670214808922 1103 28-29 0.2702729173401437 1103 30-31 0.21815444268513318 1103 32-33 0.2101341244966477 1103 34-35 0.19419302182176068 1103 36-37 0.2571353910869192 1103 38-39 0.2621193324215838 1103 40-41 0.27203347091695207 1103 42-43 0.2615016477009391 1103 44-45 0.24464403043310057 1103 46-47 0.2587964728485801 1103 48-49 0.2402045001830757 1103 50-51 0.3089760950281999 1103 52-53 0.2516662091128268 1103 54-55 0.30946526896160975 1103 56-57 0.32345634222308917 1103 58-59 0.28029897444992 1103 60-61 0.25478941697112845 1103 62-63 0.24792422444674145 1103 64-65 0.264619932747685 1103 66-67 0.25551910748579587 1103 68-69 0.21651870570143927 1103 70-71 0.16587988150797628 1103 72-73 0.21369149642447383 1103 74-75 0.22194446215807062 1103 76-77 0.17704600394866077 1103 78-79 0.21767944925005622 1103 80-81 0.2815438609352938 1103 82-83 0.27496518656202795 1103 84-85 0.28925223653366317 1103 86-87 0.25663336591858865 1103 88-89 0.2671244491990663 1103 90-91 0.25680570416924553 1103 92-93 0.24318255548016054 1103 94-95 0.23013233784082843 1103 96-97 0.239489361041354 1103 98-99 0.20894507834319853 1103 100 0.18139775277229475 1104 1 -0.08760980829599063 1104 2 -0.012197286123630136 1104 3 0.012861479252642027 1104 4 -0.04730963011952127 1104 5 -0.061875999256315595 1104 6 -0.043642125024057066 1104 7 0.006348939868729531 1104 8 0.070327256080148 1104 9 0.0683707449310802 1104 10-11 0.06850550939267919 1104 12-13 0.1259496729494174 1104 14-15 0.1370011407974232 1104 16-17 0.10456412342151822 1104 18-19 0.12348280938055467 1104 20-21 0.1269679069236389 1104 22-23 0.1002505896725836 1104 24-25 0.009393583884822476 1104 26-27 -0.003190882497705161 1104 28-29 -0.013686763669099378 1104 30-31 0.024452023735619832 1104 32-33 -0.0035153418194013852 1104 34-35 0.04177837492152037 1104 36-37 0.05773525012186553 1104 38-39 0.022057997860464695 1104 40-41 0.0700058278579263 1104 42-43 0.07039196926164948 1104 44-45 0.03554980566245547 1104 46-47 0.06785680839778507 1104 48-49 0.09251056576650285 1104 50-51 0.05300776550124198 1104 52-53 0.08086585004753033 1104 54-55 0.08383722912031288 1104 56-57 0.12581268221520503 1104 58-59 0.12477836735534709 1104 60-61 0.11906844976250142 1104 62-63 0.11186639489719852 1104 64-65 0.08969228505137039 1104 66-67 0.07073510692941198 1104 68-69 0.09060020304439576 1104 70-71 0.025785551836364107 1104 72-73 0.06340511147961081 1104 74-75 0.06003571024005083 1104 76-77 -0.005283227082820474 1104 78-79 0.02300479338792627 1104 80-81 0.05278679525373775 1104 82-83 0.037891559981083844 1104 84-85 0.01857886297162281 1104 86-87 0.0050774255892278575 1104 88-89 0.03385823701624702 1104 90-91 0.02028299492317842 1104 92-93 0.011356743951836279 1104 94-95 0.05585041948760505 1104 96-97 0.05990309324193177 1104 98-99 0.10485880765603639 1104 100 0.10538512802237321 1105 1 -0.02538490623438605 1105 2 0.021292433515466058 1105 3 0.04149548364747346 1105 4 -0.02169660148854291 1105 5 -0.06540630078436038 1105 6 0.0797080251981086 1105 7 0.003096532048417089 1105 8 0.1029179715895765 1105 9 0.16648516280002212 1105 10-11 0.16926454059231588 1105 12-13 0.1423128670207774 1105 14-15 0.15910138036891652 1105 16-17 0.14627536483533277 1105 18-19 0.20063967913344527 1105 20-21 0.16483489055945455 1105 22-23 0.16576906941968161 1105 24-25 0.13002187683994748 1105 26-27 0.07467943300342483 1105 28-29 0.09043000379897848 1105 30-31 0.1565507530152246 1105 32-33 0.14648660657243084 1105 34-35 0.09970409853579554 1105 36-37 0.17740655987927312 1105 38-39 0.1759364504914842 1105 40-41 0.2395949362323293 1105 42-43 0.24268680590802205 1105 44-45 0.20205383733477333 1105 46-47 0.17319684312297312 1105 48-49 0.159919031701925 1105 50-51 0.14185654262596614 1105 52-53 0.23232320101861603 1105 54-55 0.20137754007903652 1105 56-57 0.1893748958650079 1105 58-59 0.08477889768685998 1105 60-61 0.15222188158377747 1105 62-63 0.1276525537061275 1105 64-65 0.1703948474748458 1105 66-67 0.17168366246780664 1105 68-69 0.21972368035888934 1105 70-71 0.13407952150600977 1105 72-73 0.21884959845304763 1105 74-75 0.21717421438240336 1105 76-77 0.25649627721863055 1105 78-79 0.22073053590968073 1105 80-81 0.25956987352836336 1105 82-83 0.2745177638912395 1105 84-85 0.26117274894112086 1105 86-87 0.14835961267635156 1105 88-89 0.13989369774749605 1105 90-91 0.19862094752075876 1105 92-93 0.20632284885139995 1105 94-95 0.27612124595161447 1105 96-97 0.3306930796608363 1105 98-99 0.28928485062210996 1105 100 0.297668043738863 1106 1 -0.07172533762553357 1106 2 0.00740986947230482 1106 3 0.013046507267112872 1106 4 -0.07047064021276839 1106 5 -0.05212584408411658 1106 6 -0.03532389529898694 1106 7 -0.01077009906603621 1106 8 -0.005198424027888393 1106 9 0.032681738859544396 1106 10-11 0.017752431347929587 1106 12-13 0.054375536540362646 1106 14-15 0.07385633236249589 1106 16-17 0.04815088254623845 1106 18-19 0.07440122722837827 1106 20-21 0.09746611866260224 1106 22-23 0.08144131224713647 1106 24-25 0.09866730470137242 1106 26-27 0.045629015884159685 1106 28-29 -0.008140764412964074 1106 30-31 0.05420585865557115 1106 32-33 0.019320121673736423 1106 34-35 0.025359949461275733 1106 36-37 0.06706608914756629 1106 38-39 0.11394642988140191 1106 40-41 0.11563782181903548 1106 42-43 0.09423505383226427 1106 44-45 0.04457158976460107 1106 46-47 0.013395851740739317 1106 48-49 0.03194202189082773 1106 50-51 0.10578934238468207 1106 52-53 0.03201553594669804 1106 54-55 0.07037504413876405 1106 56-57 0.07931468294513166 1106 58-59 0.09195402658184548 1106 60-61 0.04188212116782353 1106 62-63 0.023465635755243852 1106 64-65 0.07522963895892332 1106 66-67 0.11609426431287773 1106 68-69 0.12711190397940442 1106 70-71 0.08277448569516821 1106 72-73 0.10703524439233547 1106 74-75 0.17284610751594442 1106 76-77 0.1601352903824207 1106 78-79 0.07952550162875127 1106 80-81 0.16851377606866436 1106 82-83 0.1515909242280813 1106 84-85 0.17017189219104978 1106 86-87 0.1706979830590214 1106 88-89 0.16522000587592345 1106 90-91 0.08397107854670338 1106 92-93 0.1705048273758294 1106 94-95 0.14036203862028174 1106 96-97 0.11493709607021785 1106 98-99 0.11644034201854936 1106 100 0.18159368570038126 1107 1 0.009794905728075065 1107 2 0.11327373204850133 1107 3 0.12167167294655101 1107 4 0.13679125619619725 1107 5 0.08348917046021143 1107 6 0.1677008139669951 1107 7 0.1878820277566291 1107 8 0.1511923052326125 1107 9 0.2024191706590699 1107 10-11 0.16360214724777933 1107 12-13 0.2027807446514629 1107 14-15 0.27281593154223316 1107 16-17 0.21988059681365257 1107 18-19 0.24542854178761786 1107 20-21 0.18286539764708465 1107 22-23 0.1891092502971361 1107 24-25 0.11226669216935647 1107 26-27 0.09169116190886939 1107 28-29 0.09225787188288592 1107 30-31 0.15624401997568071 1107 32-33 0.08726722660191655 1107 34-35 0.13230269890914315 1107 36-37 0.2164146320256819 1107 38-39 0.20159534903134357 1107 40-41 0.23142911201247784 1107 42-43 0.22542788350121157 1107 44-45 0.2966673630812551 1107 46-47 0.26968499062077456 1107 48-49 0.20995044232443405 1107 50-51 0.24807426895718976 1107 52-53 0.23561211055432096 1107 54-55 0.24880694076265542 1107 56-57 0.2985326053188899 1107 58-59 0.27430424354466254 1107 60-61 0.23107648066149267 1107 62-63 0.2639166501286425 1107 64-65 0.23882418849106557 1107 66-67 0.2807470548150306 1107 68-69 0.3243150439293814 1107 70-71 0.3029131943752503 1107 72-73 0.2983009993582897 1107 74-75 0.30382695865646525 1107 76-77 0.25632810039856224 1107 78-79 0.2483693265367819 1107 80-81 0.22245551670027908 1107 82-83 0.2624448332844196 1107 84-85 0.24218001983962978 1107 86-87 0.22043173351135437 1107 88-89 0.19462468302670288 1107 90-91 0.25605162679145366 1107 92-93 0.2337918857343766 1107 94-95 0.21310544261534403 1107 96-97 0.21137823185531346 1107 98-99 0.24555627761052534 1107 100 0.24149170100933404 1108 1 0.02620929423984819 1108 2 0.13507458746970968 1108 3 0.14609257384791263 1108 4 0.14273166346296762 1108 5 0.12726726052090953 1108 6 0.13244009195872763 1108 7 0.10959661777835805 1108 8 0.07922974419195583 1108 9 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-0.0740221699990613 2214 88-89 -0.11510406307944976 2214 90-91 -0.11757866190873045 2214 92-93 -0.06262804351411688 2214 94-95 -0.06239656832183371 2214 96-97 -0.12737036602269924 2214 98-99 -0.12091304088444588 2214 100 -0.1056805844923403 2215 1 -0.10526216821028811 2215 2 -0.1524861084426874 2215 3 -0.17415798003387906 2215 4 -0.11604306623982552 2215 5 -0.14635034584317452 2215 6 -0.1385561442447809 2215 7 -0.08880282992856081 2215 8 -0.10376355723239072 2215 9 -0.14635967558298546 2215 10-11 -0.17584381239222324 2215 12-13 -0.24011587872544737 2215 14-15 -0.3206539129320163 2215 16-17 -0.3095052382628154 2215 18-19 -0.26032025387950597 2215 20-21 -0.2543052275982589 2215 22-23 -0.2778937302239086 2215 24-25 -0.24891812108459987 2215 26-27 -0.24423880844338441 2215 28-29 -0.27875071094425863 2215 30-31 -0.30032118077065206 2215 32-33 -0.283265072576782 2215 34-35 -0.23263273682707108 2215 36-37 -0.18864972069340524 2215 38-39 -0.22097040816655777 2215 40-41 -0.20972209644175166 2215 42-43 -0.2132686362530265 2215 44-45 -0.12478929058831056 2215 46-47 -0.1557613686949466 2215 48-49 -0.17274579687634883 2215 50-51 -0.14675388760122132 2215 52-53 -0.1709495028320589 2215 54-55 -0.20651875523652308 2215 56-57 -0.24493868368389116 2215 58-59 -0.17761631875073647 2215 60-61 -0.2941931840613847 2215 62-63 -0.3008633002535177 2215 64-65 -0.4251594775196992 2215 66-67 -0.31060595632865073 2215 68-69 -0.2996956936414037 2215 70-71 -0.2991597246034061 2215 72-73 -0.4188097731139493 2215 74-75 -0.3640224813525421 2215 76-77 -0.29661012197695413 2215 78-79 -0.29227996302218173 2215 80-81 -0.38124309590880046 2215 82-83 -0.3303201360361214 2215 84-85 -0.37311962877708993 2215 86-87 -0.3412730141678466 2215 88-89 -0.3264432521231413 2215 90-91 -0.32178292498913663 2215 92-93 -0.3210944819007331 2215 94-95 -0.40584261610946015 2215 96-97 -0.3664673421571898 2215 98-99 -0.31034748995264394 2215 100 -0.304678614244299 2216 1 -0.43676374706798526 2216 2 -0.47650240981581504 2216 3 -0.36201401707667813 2216 4 -0.4010662841617503 2216 5 -0.31457573542406436 2216 6 -0.39597038114322913 2216 7 -0.37230843035629135 2216 8 -0.28611912069450085 2216 9 -0.4015166063900537 2216 10-11 -0.3463264035828004 2216 12-13 -0.47464525347494657 2216 14-15 -0.529243429573242 2216 16-17 -0.6132451326192907 2216 18-19 -0.5684678338536244 2216 20-21 -0.44855362878455907 2216 22-23 -0.4883575230681956 2216 24-25 -0.4877245305767772 2216 26-27 -0.5311045610298919 2216 28-29 -0.5112219774337277 2216 30-31 -0.6052573765866214 2216 32-33 -0.650263937626967 2216 34-35 -0.7831035800960322 2216 36-37 -0.7965082067871023 2216 38-39 -0.6924106576632667 2216 40-41 -0.6497351998391991 2216 42-43 -0.7109607610219939 2216 44-45 -0.5871584394567506 2216 46-47 -0.578726500646269 2216 48-49 -0.6131750996170737 2216 50-51 -0.5504271435335895 2216 52-53 -0.5651854498004951 2216 54-55 -0.6468777696593122 2216 56-57 -0.7716111674268902 2216 58-59 -0.6625844887510866 2216 60-61 -0.6086188739644527 2216 62-63 -0.7187137120019358 2216 64-65 -0.7264845700800322 2216 66-67 -0.7239467849464845 2216 68-69 -0.6881208563995003 2216 70-71 -0.6812399300450664 2216 72-73 -0.6468313662758902 2216 74-75 -0.7318239817375201 2216 76-77 -0.5728788257673116 2216 78-79 -0.5879356768961372 2216 80-81 -0.5984805483887783 2216 82-83 -0.7816699350342375 2216 84-85 -0.6587926044199008 2216 86-87 -0.6443070510994815 2216 88-89 -0.7350603380005438 2216 90-91 -0.6629087514567473 2216 92-93 -0.8098475917983379 2216 94-95 -0.7908389125816981 2216 96-97 -0.7434998140923632 2216 98-99 -0.7409531661505042 2216 100 -0.7573961761263668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45403.0 3 9011.0 4 4662.0 5 3770.0 6 3553.0 7 3825.0 8 4687.0 9 5205.0 10 5853.0 11 6528.0 12 7185.0 13 8119.0 14 9293.0 15 9721.0 16 9426.0 17 9821.0 18 9850.0 19 9488.0 20 9746.0 21 10018.0 22 11376.0 23 15783.0 24 23585.0 25 31730.0 26 33954.0 27 30250.0 28 32487.0 29 40668.0 30 49528.0 31 60113.0 32 77651.0 33 105189.0 34 149579.0 35 224807.0 36 395032.0 37 847742.0 38 1543780.0 39 739621.0 40 1343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.65453979416261 20.87519724070774 13.851350395873766 23.618912569255883 2 14.653899034227386 20.63729116453241 41.55580289024872 23.153006910991486 3 17.279044815394613 26.26859911536999 30.794213444183292 25.658142625052104 4 11.53992689550461 17.375753581408766 41.17037690439299 29.91394261869363 5 11.970321947461708 38.73553826016279 37.06668639690641 12.22745339546908 6 29.51660417112325 39.88043334208873 18.449724618471073 12.153237868316946 7 25.892000455262714 33.18678023280638 24.007926620007318 16.913292691923594 8 23.827808221742142 37.677877368161475 21.318448316914775 17.175866093181604 9 24.049049659047284 17.34425560180135 22.343611391381334 36.26308334777003 10-11 22.697969713013087 27.77901414665971 30.214215872183274 19.308800268143926 12-13 23.48222321619995 25.138186484006937 30.04777622876869 21.331814071024425 14-15 20.787139737839194 27.303886530478632 26.966314878531232 24.942658853150945 16-17 20.409342003112346 30.602551672277478 27.56644635378408 21.421659970826095 18-19 20.262719064951437 29.749523152045505 29.456079931121476 20.53167785188158 20-21 21.54592676313933 29.015770123912706 28.84456413363991 20.593738979308057 22-23 21.135582418555405 28.748100402930493 28.95223604506582 21.164081133448278 24-25 21.079261695707725 28.863315845271426 28.85592192894229 21.201500530078558 26-27 20.873897907474053 28.69294317719573 29.03364366995518 21.399515245375035 28-29 20.87635530286194 28.768454751042867 29.074208661732964 21.280981284362234 30-31 21.15256739931825 29.285580934667127 28.66172028570322 20.900131380311404 32-33 20.766406753322823 28.94297216786963 29.16039946745216 21.130221611355392 34-35 20.868879250260143 28.71349677441803 29.262362193765433 21.15526178155639 36-37 21.234573943914743 29.109634038369087 28.61625041060012 21.039541607116053 38-39 21.130857411364953 29.214944309108624 28.754923413804807 20.89927486572162 40-41 20.869082894154754 28.921484466036528 29.042831145437365 21.166601494371353 42-43 20.825735374193844 28.753571326381994 29.09699309577111 21.323700203653047 44-45 20.989539907361795 28.88786286342915 29.02622399228537 21.09637323692369 46-47 21.03045145204903 29.08892835394695 28.855423738199786 21.025196455804227 48-49 20.949423366298802 28.938478950800068 28.879032962604622 21.233064720296507 50-51 21.023688272116992 28.757703559512564 28.83949191999971 21.37911624837073 52-53 20.952068867752306 28.808463685394326 28.91556093435278 21.32390651250059 54-55 21.131735530950717 29.196004438835526 28.720105660037458 20.9521543701763 56-57 21.058822735902545 28.87428379713819 28.742455039191807 21.324438427767458 58-59 20.79163728588837 28.596332365082123 29.182243646408963 21.429786702620543 60-61 21.142922473474353 28.974647773333963 28.72321192167524 21.159217831516443 62-63 20.9822663606533 28.99993048447318 28.69839192854021 21.319411226333308 64-65 20.90347627608393 28.646963439259103 28.988359906698236 21.46120037795873 66-67 20.85949302354473 28.726899850844482 28.781401282948483 21.63220584266231 68-69 21.342297941407317 28.91701826880661 28.609135001623613 21.13154878816245 70-71 21.216492714684303 28.73569995050663 28.730522182277024 21.317285152532047 72-73 20.94835038214197 28.884827240153193 28.916197874737403 21.25062450296743 74-75 21.116702369086624 28.828664389384194 28.753271059958852 21.30136218157033 76-77 21.08680580255704 28.866803239905316 28.59198168465418 21.454409272883467 78-79 21.17441386062175 29.005318834783512 28.632844862551178 21.18742244204356 80-81 21.16886650919479 28.602234435875744 28.865371551477924 21.36352750345154 82-83 20.994497312394312 28.73140160439493 28.94789365654576 21.326207426665004 84-85 21.020356980731027 28.669913000656244 28.84807926930731 21.461650749305424 86-87 21.177985093212552 28.835685370950277 28.68877953967621 21.29754999616096 88-89 20.987179817633862 28.645400513920965 28.798617566300255 21.568802102144918 90-91 20.973292201468993 28.527672547090628 28.926850721826806 21.572184529613573 92-93 21.152223595691602 28.77636711379222 28.691200169833607 21.380209120682572 94-95 20.878528106965774 28.502723649420513 28.922200896659668 21.696547346954045 96-97 20.88019124870347 28.532325422532846 28.900482004417967 21.68700132434572 98-99 21.016744207697 28.56702696835634 28.777430742181746 21.63879808176491 100 21.086382562816773 28.49511451102365 28.762181092329946 21.656321833829637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 280.0 1 266.0 2 288.5 3 344.0 4 389.0 5 423.5 6 405.5 7 352.0 8 321.0 9 324.0 10 314.5 11 291.5 12 281.0 13 301.0 14 356.5 15 436.5 16 567.0 17 781.0 18 1139.5 19 1682.5 20 2610.5 21 3981.5 22 6260.5 23 9856.5 24 14738.5 25 21874.0 26 31630.0 27 43797.0 28 58903.5 29 75697.0 30 92736.5 31 110917.0 32 128490.5 33 143406.0 34 156642.5 35 167787.0 36 175998.5 37 182477.0 38 187278.0 39 189742.0 40 188482.5 41 183697.5 42 178159.0 43 172249.5 44 165240.0 45 158231.5 46 152273.5 47 144854.0 48 138218.0 49 133296.0 50 126258.0 51 118532.5 52 110450.5 53 102568.0 54 96335.0 55 90479.0 56 82965.5 57 74595.0 58 66404.0 59 57808.0 60 49974.5 61 42208.0 62 34243.5 63 27273.5 64 21450.0 65 17016.0 66 13428.0 67 10187.5 68 7698.0 69 5860.5 70 4224.5 71 2864.0 72 2050.0 73 1604.0 74 1192.5 75 850.5 76 603.5 77 396.5 78 264.0 79 171.0 80 124.0 81 84.5 82 51.0 83 37.5 84 35.5 85 26.5 86 17.0 87 12.0 88 15.0 89 12.0 90 6.5 91 7.0 92 7.0 93 6.5 94 4.5 95 2.0 96 3.5 97 3.5 98 2.0 99 3.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06176916811867334 2 0.04180996490398058 3 0.05779037270659406 4 0.07598846975528452 5 0.08842492317446127 6 0.08959899395179613 7 0.09203410371219438 8 0.10401397405129646 9 0.10023085710210633 10-11 0.10511194764861886 12-13 0.1097321335779459 14-15 0.10762315458902956 16-17 0.07596672770385239 18-19 0.10057872992502036 20-21 0.09419743782969103 22-23 0.05834479501811331 24-25 0.021894245792152076 26-27 0.1826223610041523 28-29 0.06606322327651845 30-31 0.07748867130410128 32-33 0.07311851896624372 34-35 0.021459404763509535 36-37 0.05331151011157586 38-39 0.07854316079855946 40-41 0.08416348109376434 42-43 0.07773870489557075 44-45 0.07474917282365327 46-47 0.08165227415335365 48-49 0.07856490284999158 50-51 0.07334681050628106 52-53 0.07737996104694066 54-55 0.06808523405970629 56-57 0.07105302408019164 58-59 0.0679004266225332 60-61 0.06481305531917114 62-63 0.071455252031686 64-65 0.06768300610821193 66-67 0.0689766581684235 68-69 0.06869401149980583 70-71 0.06102993836998101 72-73 0.05927970322969477 74-75 0.06134519811574685 76-77 0.056116234746320263 78-79 0.05253966728573534 80-81 0.0469410890419626 82-83 0.05319192882869916 84-85 0.043288424401365225 86-87 0.04085331464096698 88-89 0.04310361696419215 90-91 0.039374855143582334 92-93 0.042201321829758866 94-95 0.040744604383806345 96-97 0.0438645887643166 98-99 0.044158106458650315 100 0.05220266548853738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4599382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.24400544261805 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33410095308068 38.99627551364099 2 15.77250903395181 17.742181678986558 3 6.47861921093201 10.931504824809295 4 3.1114680814858597 7.0000571083849215 5 1.7739898392923183 4.988814708815314 6 1.0242575443002646 3.4565008137760014 7 0.6471095029821352 2.5477221285387954 8 0.44552092785216624 2.004630519273398 9 0.2918611854793119 1.4773897894128636 >10 1.090820465818732 9.471285357277255 >50 0.0237957859371156 0.9004093349683581 >100 0.005947468887620211 0.48322822211620803 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.1742051432127186E-5 0.0 0.0 0.0 0.0 9 2.1742051432127186E-5 0.0 0.0 0.0 0.0 10-11 2.1742051432127186E-5 0.0 0.0 0.0 0.0 12-13 2.1742051432127186E-5 0.0 0.0 0.0 0.0 14-15 2.1742051432127186E-5 0.0 0.0 0.0 0.0 16-17 2.1742051432127186E-5 0.0 0.0 0.0 0.0 18-19 2.1742051432127186E-5 0.0 0.0 2.1742051432127186E-5 0.0 20-21 2.1742051432127186E-5 0.0 0.0 2.1742051432127186E-5 0.0 22-23 2.1742051432127186E-5 0.0 0.0 5.435512858031796E-5 0.0 24-25 2.1742051432127186E-5 0.0 0.0 6.522615429638155E-5 0.0 26-27 2.1742051432127186E-5 0.0 0.0 1.9567846288914468E-4 0.0 28-29 2.1742051432127186E-5 0.0 0.0 6.196484658156248E-4 0.0 30-31 2.1742051432127186E-5 0.0 0.0 0.002772111557596216 0.0 32-33 2.1742051432127186E-5 0.0 0.0 0.007946719798442487 0.0 34-35 2.1742051432127186E-5 0.0 0.0 0.014817208050994677 0.0 36-37 2.1742051432127186E-5 0.0 0.0 0.02826466686176534 0.0 38-39 2.1742051432127186E-5 0.0 0.0 0.052409214977142585 0.0 40-41 2.1742051432127186E-5 0.0 0.0 0.08065213978747579 0.0 42-43 2.1742051432127186E-5 0.0 0.0 0.11134104538392331 0.0 44-45 2.1742051432127186E-5 0.0 0.0 0.1455956474152397 0.0 46-47 2.1742051432127186E-5 0.0 0.0 0.188894942842321 0.0 48-49 2.1742051432127186E-5 0.0 0.0 0.23858640139044768 0.0 50-51 2.1742051432127186E-5 0.0 0.0 0.29335462894797604 0.0 52-53 2.1742051432127186E-5 0.0 0.0 0.3488512152284807 0.0 54-55 2.1742051432127186E-5 0.0 0.0 0.4089571164125963 0.0 56-57 2.1742051432127186E-5 0.0 0.0 0.4814125028101601 0.0 58-59 2.1742051432127186E-5 0.0 0.0 0.5638040067122061 0.0 60-61 2.1742051432127186E-5 0.0 0.0 0.651989767320914 0.0 62-63 2.1742051432127186E-5 0.0 0.0 0.7451870707847272 0.0 64-65 2.1742051432127186E-5 0.0 0.0 0.8461245445583776 0.0 66-67 2.1742051432127186E-5 0.0 0.0 0.9613030620200714 0.0 68-69 2.1742051432127186E-5 0.0 0.0 1.0919075649728593 0.0 70-71 2.1742051432127186E-5 0.0 0.0 1.2375684385423955 0.0 72-73 2.1742051432127186E-5 0.0 0.0 1.3908933852417564 0.0 74-75 2.1742051432127186E-5 0.0 0.0 1.5477405442731218 0.0 76-77 2.1742051432127186E-5 0.0 0.0 1.7189918123782717 0.0 78-79 2.1742051432127186E-5 0.0 0.0 1.9163770263048385 0.0 80-81 2.1742051432127186E-5 0.0 0.0 2.1385699209154625 0.0 82-83 2.1742051432127186E-5 0.0 0.0 2.3722643607336815 0.0 84-85 2.1742051432127186E-5 0.0 0.0 2.6034367225857737 0.0 86-87 2.1742051432127186E-5 0.0 0.0 2.848143511454365 0.0 88 2.1742051432127186E-5 0.0 0.0 3.0496488441273195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 4415 0.0 33.64677 4 ATCAACG 4490 0.0 33.39884 3 CAACGCA 4635 0.0 32.45684 5 GGTATCA 3565 0.0 30.20089 1 GTATCAA 5350 0.0 29.703438 1 AACGCAG 5050 0.0 29.597933 6 GTACATG 9665 0.0 25.246971 1 TATCAAC 6215 0.0 25.112156 2 CGCAGAG 5925 0.0 24.454123 8 ACGCAGA 6040 0.0 24.435335 7 TACATGG 9690 0.0 23.86868 2 ACATGGG 9940 0.0 22.653545 3 CATGGGG 6625 0.0 18.16524 4 ATTCGCG 80 0.0012783163 17.62747 16-17 GCAGAGT 7830 0.0 17.485296 9 CTGTCGC 1085 0.0 16.04404 9 GAGTACA 7330 0.0 15.842976 1 GTATTAA 3720 0.0 15.039999 1 GTATTAT 3665 0.0 15.009135 1 GTGTAGG 1845 0.0 14.780034 1 >>END_MODULE