##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033072_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4599382 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68786502186598 34.0 31.0 34.0 31.0 34.0 2 32.695012721274296 34.0 31.0 34.0 31.0 34.0 3 32.806764908850795 34.0 31.0 34.0 31.0 34.0 4 36.298642730697296 37.0 37.0 37.0 35.0 37.0 5 36.27814562913017 37.0 37.0 37.0 35.0 37.0 6 36.34541097043037 37.0 37.0 37.0 35.0 37.0 7 36.29720601593866 37.0 37.0 37.0 35.0 37.0 8 36.30166509326688 37.0 37.0 37.0 35.0 37.0 9 38.097941419086304 39.0 39.0 39.0 37.0 39.0 10-11 38.06939530136875 39.0 39.0 39.0 35.0 39.0 12-13 38.04092517212095 39.0 39.0 39.0 35.0 39.0 14-15 39.53785410735617 41.0 39.0 41.0 37.0 41.0 16-17 39.53927538525828 41.0 39.0 41.0 37.0 41.0 18-19 39.55888454144491 41.0 39.5 41.0 37.0 41.0 20-21 39.557456849637624 41.0 40.0 41.0 37.0 41.0 22-23 39.48388587858108 41.0 39.0 41.0 36.5 41.0 24-25 39.4331711303823 41.0 39.0 41.0 36.0 41.0 26-27 39.328796020856714 41.0 39.0 41.0 36.0 41.0 28-29 39.28297627811736 41.0 39.0 41.0 36.0 41.0 30-31 39.28689702225212 41.0 39.0 41.0 36.0 41.0 32-33 39.25152172617973 41.0 39.0 41.0 35.5 41.0 34-35 39.17557141372471 41.0 39.0 41.0 35.0 41.0 36-37 39.08856189809848 41.0 39.0 41.0 35.0 41.0 38-39 39.00032602206122 41.0 39.0 41.0 35.0 41.0 40-41 38.90663267369399 41.0 39.0 41.0 35.0 41.0 42-43 38.79971102639442 41.0 39.0 41.0 35.0 41.0 44-45 38.65358345969089 41.0 39.0 41.0 35.0 41.0 46-47 38.483414184775256 41.0 38.5 41.0 35.0 41.0 48-49 38.306348113724844 40.5 38.0 41.0 34.0 41.0 50-51 38.19091651878448 40.0 38.0 41.0 34.0 41.0 52-53 38.07640156873249 40.0 38.0 41.0 34.0 41.0 54-55 37.94912609998474 40.0 38.0 41.0 34.0 41.0 56-57 37.8208390822941 40.0 38.0 41.0 33.5 41.0 58-59 37.66355751707512 40.0 37.0 41.0 33.0 41.0 60-61 37.49775513318963 40.0 37.0 41.0 33.0 41.0 62-63 37.29412799371741 40.0 36.5 41.0 33.0 41.0 64-65 37.049201392708845 39.0 36.0 41.0 32.5 41.0 66-67 36.793490516769424 39.0 35.5 41.0 32.0 41.0 68-69 36.50408043950252 39.0 35.0 41.0 32.0 41.0 70-71 36.189887684910715 38.0 35.0 40.0 31.5 41.0 72-73 35.85063493312797 37.0 35.0 40.0 31.0 41.0 74-75 35.501242884370114 37.0 35.0 39.0 31.0 41.0 76-77 35.133007434477065 36.0 35.0 39.0 31.0 41.0 78-79 34.75375093871307 36.0 35.0 38.5 31.0 40.0 80-81 34.39353624030359 35.5 34.5 37.0 30.0 39.0 82-83 34.060806212660744 35.0 34.0 37.0 30.0 39.0 84-85 33.80291536993448 35.0 34.0 36.5 30.0 38.5 86-87 33.557666008172404 35.0 34.0 36.0 30.0 37.0 88-89 33.32257311960607 35.0 34.0 36.0 30.0 37.0 90-91 33.11467029700947 35.0 34.0 35.5 30.0 36.5 92-93 32.91941395604888 35.0 34.0 35.0 30.0 36.0 94-95 32.7507143350998 35.0 34.0 35.0 29.0 36.0 96-97 32.59816894965454 35.0 34.0 35.0 29.0 36.0 98-99 32.46410332953427 35.0 34.0 35.0 29.0 36.0 100 32.35510227243573 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13727181719679749 1101 2 0.09918353067362773 1101 3 0.1817538791345612 1101 4 0.14972344401859772 1101 5 0.11351340332799253 1101 6 0.10550871035555787 1101 7 0.15424146892451063 1101 8 0.13723940035213644 1101 9 0.20626715236669924 1101 10-11 0.20833634661862277 1101 12-13 0.22961536868547228 1101 14-15 0.34521475388904577 1101 16-17 0.3011593251672977 1101 18-19 0.3163944137499328 1101 20-21 0.30519268174738556 1101 22-23 0.31652968007882976 1101 24-25 0.3635508972406498 1101 26-27 0.36703966851241177 1101 28-29 0.345563395975347 1101 30-31 0.3757516810940871 1101 32-33 0.31765514927881355 1101 34-35 0.3028823330396335 1101 36-37 0.3521383463184691 1101 38-39 0.3238446565165205 1101 40-41 0.30512870978600404 1101 42-43 0.30557751137102684 1101 44-45 0.33680321139531344 1101 46-47 0.3262684644796252 1101 48-49 0.3429924114889715 1101 50-51 0.29251431011360296 1101 52-53 0.3012482633142284 1101 54-55 0.30415333772029385 1101 56-57 0.2867903929110227 1101 58-59 0.2378429874355632 1101 60-61 0.20569957725173538 1101 62-63 0.23151077019021216 1101 64-65 0.231686754726681 1101 66-67 0.232993557708852 1101 68-69 0.2606912271003239 1101 70-71 0.2146168090863796 1101 72-73 0.24617724578313016 1101 74-75 0.20683296987338906 1101 76-77 0.1910383566389413 1101 78-79 0.186210077328667 1101 80-81 0.23546344695989063 1101 82-83 0.20834869257416955 1101 84-85 0.26800369894829146 1101 86-87 0.2855526497795182 1101 88-89 0.29054121688475476 1101 90-91 0.27995361412828856 1101 92-93 0.28026770859629124 1101 94-95 0.2597989417838704 1101 96-97 0.30224908561732633 1101 98-99 0.33913234366406186 1101 100 0.309867729906415 1102 1 0.12638735138088464 1102 2 0.0816041274533319 1102 3 0.11012242775934311 1102 4 0.10656691991392364 1102 5 0.13565075977420094 1102 6 0.1294812892806334 1102 7 0.13230833689112842 1102 8 0.15740954135220875 1102 9 0.19881792835411005 1102 10-11 0.1811515859007784 1102 12-13 0.2207021834585845 1102 14-15 0.27717040550687244 1102 16-17 0.2906061750318756 1102 18-19 0.24831370129220431 1102 20-21 0.23421684249929342 1102 22-23 0.2219639473981303 1102 24-25 0.282048011041482 1102 26-27 0.29419745811828335 1102 28-29 0.3213904224987445 1102 30-31 0.2863642346003843 1102 32-33 0.30311740098942863 1102 34-35 0.2757424065342775 1102 36-37 0.2753469476484227 1102 38-39 0.278287162298831 1102 40-41 0.30231695110272483 1102 42-43 0.3108324732112351 1102 44-45 0.31318609065523617 1102 46-47 0.3196057606519247 1102 48-49 0.32646019430357853 1102 50-51 0.35519967605411296 1102 52-53 0.37303898724898943 1102 54-55 0.3299543290295688 1102 56-57 0.3281959776278427 1102 58-59 0.3348927707699758 1102 60-61 0.3415522081625397 1102 62-63 0.3358351332476843 1102 64-65 0.36848428954038326 1102 66-67 0.36632701565427794 1102 68-69 0.3781328520406717 1102 70-71 0.3784756340723874 1102 72-73 0.3695214581882311 1102 74-75 0.403557769216107 1102 76-77 0.3519919769401696 1102 78-79 0.42844456951441856 1102 80-81 0.39639161763670216 1102 82-83 0.37683538862942356 1102 84-85 0.4193556739724684 1102 86-87 0.453160294632319 1102 88-89 0.49395993407832606 1102 90-91 0.4392548257395461 1102 92-93 0.4377367433180481 1102 94-95 0.47015077976959674 1102 96-97 0.5127824302720541 1102 98-99 0.47873315395291627 1102 100 0.4860798051950539 1103 1 0.10987571901733872 1103 2 0.12446440200734799 1103 3 0.13263290967907437 1103 4 0.07750292529917147 1103 5 0.10278095068592563 1103 6 0.12228704073218921 1103 7 0.13312752286304175 1103 8 0.11013904942371511 1103 9 0.15079418803414768 1103 10-11 0.16465213928054823 1103 12-13 0.19119136579545426 1103 14-15 0.26222636062107796 1103 16-17 0.26330551229938237 1103 18-19 0.2490316758193174 1103 20-21 0.275418323398533 1103 22-23 0.2536205025144014 1103 24-25 0.24320539350494386 1103 26-27 0.25600169422655483 1103 28-29 0.2940594947657118 1103 30-31 0.2567712939983622 1103 32-33 0.2299590948017638 1103 34-35 0.2418231158771107 1103 36-37 0.27806581375329387 1103 38-39 0.2981194969369838 1103 40-41 0.29116520174208205 1103 42-43 0.2835423390606522 1103 44-45 0.29507754030290556 1103 46-47 0.2789286778909599 1103 48-49 0.281853097182136 1103 50-51 0.29437968417843763 1103 52-53 0.2518251680977315 1103 54-55 0.2825402122919911 1103 56-57 0.2837260386480125 1103 58-59 0.2947937383055361 1103 60-61 0.3094087590873187 1103 62-63 0.26549676755123386 1103 64-65 0.3048176806127145 1103 66-67 0.25261183943733556 1103 68-69 0.22853613161544217 1103 70-71 0.2700954460575744 1103 72-73 0.30400069745694225 1103 74-75 0.2501382141482438 1103 76-77 0.2634582904246159 1103 78-79 0.26055904193864166 1103 80-81 0.22139169675681813 1103 82-83 0.29145291290952 1103 84-85 0.32807184710282655 1103 86-87 0.31081711923045674 1103 88-89 0.2695478208115176 1103 90-91 0.26685585084714347 1103 92-93 0.29613676120043664 1103 94-95 0.3062061440123074 1103 96-97 0.2953305070789227 1103 98-99 0.2934022645464083 1103 100 0.3319802657139803 1104 1 0.04417644656508202 1104 2 0.0359624488944803 1104 3 0.04591175975766504 1104 4 0.05381463982723744 1104 5 0.071737007766842 1104 6 0.06933943362802353 1104 7 0.10180292576695393 1104 8 0.07131817781004912 1104 9 0.10727708324465368 1104 10-11 0.13029541863882343 1104 12-13 0.139204662143527 1104 14-15 0.1568785477167367 1104 16-17 0.1762045827115628 1104 18-19 0.20247028032574832 1104 20-21 0.16405086020708382 1104 22-23 0.15363043197896076 1104 24-25 0.1738520466821356 1104 26-27 0.19820140869585146 1104 28-29 0.23355285622149324 1104 30-31 0.2061706677195616 1104 32-33 0.19084546226556398 1104 34-35 0.1263209091025459 1104 36-37 0.18117435720329667 1104 38-39 0.18919623101260896 1104 40-41 0.18285060788678464 1104 42-43 0.22558273262509232 1104 44-45 0.1722232041946654 1104 46-47 0.1529166439792249 1104 48-49 0.07929565713872933 1104 50-51 0.15180153845869881 1104 52-53 0.17369861133276032 1104 54-55 0.15949665089246423 1104 56-57 0.21485238234875936 1104 58-59 0.18843665537972498 1104 60-61 0.24144587561431052 1104 62-63 0.22933180417800259 1104 64-65 0.21117828681896356 1104 66-67 0.20118174401564204 1104 68-69 0.2252313042306895 1104 70-71 0.27817916355051153 1104 72-73 0.23386066590835952 1104 74-75 0.2324078490602659 1104 76-77 0.23993075160201727 1104 78-79 0.15460780080510972 1104 80-81 0.19949975267471132 1104 82-83 0.22453613924556493 1104 84-85 0.22910759972398154 1104 86-87 0.21430608272363827 1104 88-89 0.2217727301190635 1104 90-91 0.22586080347267767 1104 92-93 0.19736061543290617 1104 94-95 0.2538954338849919 1104 96-97 0.31120326001197185 1104 98-99 0.2991452720732042 1104 100 0.2856144267838161 1105 1 0.052640053142113175 1105 2 0.08407250882194006 1105 3 0.09815861293193251 1105 4 0.0738842285436263 1105 5 0.060900605812456376 1105 6 0.08711900479735846 1105 7 0.12085217972887108 1105 8 0.11005273766949131 1105 9 0.13792540197849945 1105 10-11 0.1349878609537427 1105 12-13 0.13511571402148093 1105 14-15 0.19642443407361299 1105 16-17 0.19817692015919164 1105 18-19 0.15885291219959186 1105 20-21 0.1770850250848497 1105 22-23 0.19041272628528105 1105 24-25 0.17417137542079786 1105 26-27 0.2107659419695409 1105 28-29 0.22036834236254776 1105 30-31 0.16167125454289533 1105 32-33 0.14204297385791875 1105 34-35 0.17507543709598394 1105 36-37 0.1385783729656751 1105 38-39 0.15738439954734673 1105 40-41 0.12736585030592096 1105 42-43 0.08261702662704806 1105 44-45 0.08811487591485445 1105 46-47 0.09074117803687898 1105 48-49 0.12453243862601937 1105 50-51 0.11373442385406918 1105 52-53 0.11583924833006876 1105 54-55 0.09196461771676923 1105 56-57 0.08232960691648827 1105 58-59 0.09063148995087289 1105 60-61 0.09840299485358628 1105 62-63 0.09614618354027016 1105 64-65 0.0966903795301377 1105 66-67 0.08864364661449997 1105 68-69 0.09822383808543833 1105 70-71 0.11497147651066086 1105 72-73 0.12129539478214468 1105 74-75 0.11276885493772681 1105 76-77 0.09060720096343289 1105 78-79 0.0683576019307921 1105 80-81 0.09991051748190927 1105 82-83 0.04762782203354021 1105 84-85 0.06960106165885804 1105 86-87 0.0286962360816716 1105 88-89 0.05799430262114669 1105 90-91 0.14325825926493252 1105 92-93 0.1526618667846762 1105 94-95 0.1046605869548003 1105 96-97 0.10166821086407651 1105 98-99 0.06668410732687846 1105 100 0.0625685023814242 1106 1 0.011218656683197992 1106 2 -0.004172093324740445 1106 3 0.04739558082347628 1106 4 0.04019559162862407 1106 5 0.023689786282922398 1106 6 0.08229908623233229 1106 7 0.07207693893183631 1106 8 0.05275628684410094 1106 9 0.06627377579254556 1106 10-11 0.10854857546581798 1106 12-13 0.05643091281153545 1106 14-15 0.10115309345644619 1106 16-17 0.1659876723726441 1106 18-19 0.15325470612934566 1106 20-21 0.1373447165236854 1106 22-23 0.15137497464225902 1106 24-25 0.08717786879956435 1106 26-27 0.07518169087182969 1106 28-29 0.10703443713396155 1106 30-31 0.05038583437473676 1106 32-33 0.06676611463375792 1106 34-35 0.02546720355290688 1106 36-37 -0.007063380924130058 1106 38-39 0.022712609136391393 1106 40-41 0.028245155638977337 1106 42-43 0.018424415222213497 1106 44-45 0.007903453163329743 1106 46-47 -0.0784492149972209 1106 48-49 -0.07377964351708499 1106 50-51 -0.050531787190578825 1106 52-53 -0.06728626118488279 1106 54-55 -0.0038065884867961586 1106 56-57 -0.06082140839792771 1106 58-59 -0.07597785605594964 1106 60-61 -0.0768790442115943 1106 62-63 -0.0715799864590565 1106 64-65 -0.09599451566003836 1106 66-67 -0.04624863673373625 1106 68-69 -0.06903118493972471 1106 70-71 -0.06575150471732627 1106 72-73 -0.05657032494255532 1106 74-75 -0.04720973723830468 1106 76-77 -0.09148401232144465 1106 78-79 -0.0087493475799576 1106 80-81 -0.038133961397669225 1106 82-83 -0.05384726336826873 1106 84-85 -0.0552010880844378 1106 86-87 -0.11323499224003797 1106 88-89 -0.07400577168881028 1106 90-91 -0.09389859812333867 1106 92-93 -0.05235277664774429 1106 94-95 -0.046409082999716134 1106 96-97 -0.08685845220365707 1106 98-99 -0.04509883984967189 1106 100 -0.05150005510935074 1107 1 1.5124077902584077E-4 1107 2 0.0245581161934183 1107 3 0.029678512008793234 1107 4 -0.015631783628521134 1107 5 0.002409935236151739 1107 6 0.033509297764275914 1107 7 0.052152588024348745 1107 8 0.006502442315877488 1107 9 0.02877412807957569 1107 10-11 0.06132023392611785 1107 12-13 0.024051929574213204 1107 14-15 0.05773532678311 1107 16-17 0.08594714953771643 1107 18-19 0.05494444198641446 1107 20-21 0.04339617351175917 1107 22-23 0.06941321214836904 1107 24-25 0.06838530008644739 1107 26-27 0.04864642817359055 1107 28-29 0.09458252274561119 1107 30-31 0.10098686950065883 1107 32-33 0.08696485218757033 1107 34-35 0.0778363327888485 1107 36-37 0.05620062719992802 1107 38-39 0.0983259084505761 1107 40-41 0.08974986114834849 1107 42-43 0.10442251943712222 1107 44-45 0.18294917102979724 1107 46-47 0.21684634608584474 1107 48-49 0.20086583446804696 1107 50-51 0.20213597691116547 1107 52-53 0.2181849867993506 1107 54-55 0.23458073473305774 1107 56-57 0.236500315338958 1107 58-59 0.21529705419136036 1107 60-61 0.21718305230476176 1107 62-63 0.2627821783236328 1107 64-65 0.1883501560331169 1107 66-67 0.22677895522279812 1107 68-69 0.2019966502098569 1107 70-71 0.14392580538030586 1107 72-73 0.20010464663822347 1107 74-75 0.17041297301098268 1107 76-77 0.2135698576968963 1107 78-79 0.1566363253894636 1107 80-81 0.16636473980263133 1107 82-83 0.10939883561213293 1107 84-85 0.14103337752101197 1107 86-87 0.13774023652491962 1107 88-89 0.15197906186320154 1107 90-91 0.1250118414397221 1107 92-93 0.18229921687034079 1107 94-95 0.17093163036709313 1107 96-97 0.13473528554208514 1107 98-99 0.13447185334833733 1107 100 0.11497028558419942 1108 1 -0.009743197318947239 1108 2 0.03455740571600785 1108 3 0.034289934469384775 1108 4 0.03410135529429681 1108 5 0.003802166933716933 1108 6 0.041638143483311296 1108 7 0.03939631810117561 1108 8 0.04383162970413679 1108 9 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2214 68-69 -0.15212198567328272 2214 70-71 -0.03260338895541537 2214 72-73 -0.06576313940379208 2214 74-75 -0.11870507154398524 2214 76-77 -0.07015712790868633 2214 78-79 -0.03785713109370192 2214 80-81 -0.04569773001644961 2214 82-83 -0.0873965519157025 2214 84-85 -0.07086630147627915 2214 86-87 -0.07339650973180767 2214 88-89 -0.06799530174266266 2214 90-91 -0.06754094197432181 2214 92-93 -0.12013203385478022 2214 94-95 -0.12998723884487617 2214 96-97 -0.07718103772818807 2214 98-99 -0.12913073141595532 2214 100 -0.13352828862664978 2215 1 -0.18084284404779538 2215 2 -0.13257191232334975 2215 3 -0.15962068764024906 2215 4 -0.12321407280506236 2215 5 -0.13700801698103504 2215 6 -0.1500165115790395 2215 7 -0.14513947551683515 2215 8 -0.12461631696145758 2215 9 -0.22157820680567397 2215 10-11 -0.1716913061453056 2215 12-13 -0.18697612837287636 2215 14-15 -0.2931643577619809 2215 16-17 -0.29369762583535675 2215 18-19 -0.3151201140620472 2215 20-21 -0.2996389431821882 2215 22-23 -0.30186443979036426 2215 24-25 -0.3596638121035838 2215 26-27 -0.3574247814691276 2215 28-29 -0.38035507841417626 2215 30-31 -0.4301859363666267 2215 32-33 -0.3201859200327348 2215 34-35 -0.32765682582818556 2215 36-37 -0.32152241177043805 2215 38-39 -0.34472098697008136 2215 40-41 -0.32698855842242835 2215 42-43 -0.32829724640959057 2215 44-45 -0.39518962173594474 2215 46-47 -0.42741320707423824 2215 48-49 -0.3437406588451495 2215 50-51 -0.32597417174383025 2215 52-53 -0.316408664586767 2215 54-55 -0.3172971112791032 2215 56-57 -0.3758813343016669 2215 58-59 -0.33102572386018636 2215 60-61 -0.3056848612387668 2215 62-63 -0.28539664773609275 2215 64-65 -0.35471851343014293 2215 66-67 -0.3501321936757762 2215 68-69 -0.2838908879725963 2215 70-71 -0.2876100118757421 2215 72-73 -0.21240275913778106 2215 74-75 -0.2564790615574708 2215 76-77 -0.2881458451223722 2215 78-79 -0.28450707850304724 2215 80-81 -0.2270260595227782 2215 82-83 -0.17980890347342893 2215 84-85 -0.24373837875594262 2215 86-87 -0.20275031920232323 2215 88-89 -0.16517881524671196 2215 90-91 -0.1938939323065867 2215 92-93 -0.18735371700818604 2215 94-95 -0.1394266250313052 2215 96-97 -0.1909284855865394 2215 98-99 -0.2119043517204915 2215 100 -0.23588074025698091 2216 1 -0.2008452513060135 2216 2 -0.13736504483915724 2216 3 -0.149989634789371 2216 4 -0.14485531025280096 2216 5 -0.1879043236367579 2216 6 -0.14266132076830473 2216 7 -0.16772986646086707 2216 8 -0.16796082612108876 2216 9 -0.22847646434357927 2216 10-11 -0.2612412491609888 2216 12-13 -0.29423566967595605 2216 14-15 -0.3937193993449952 2216 16-17 -0.353735669709593 2216 18-19 -0.36131828295768287 2216 20-21 -0.3631727216261069 2216 22-23 -0.3603443829968995 2216 24-25 -0.37726288269015384 2216 26-27 -0.4357769825710349 2216 28-29 -0.3787974931895022 2216 30-31 -0.3264880506163763 2216 32-33 -0.3559441388065352 2216 34-35 -0.3236540416000224 2216 36-37 -0.280145425242587 2216 38-39 -0.2778703117884831 2216 40-41 -0.27226921087856226 2216 42-43 -0.2667912486728028 2216 44-45 -0.3062651270337966 2216 46-47 -0.20720014382562368 2216 48-49 -0.22058056405379034 2216 50-51 -0.23487836634947712 2216 52-53 -0.27284014667630174 2216 54-55 -0.23592531958104956 2216 56-57 -0.2598881682402734 2216 58-59 -0.23841670313367302 2216 60-61 -0.24140664376332666 2216 62-63 -0.28773090111200617 2216 64-65 -0.30781259947373485 2216 66-67 -0.3515315378929458 2216 68-69 -0.2610040058442422 2216 70-71 -0.26914496915178887 2216 72-73 -0.2476790386867691 2216 74-75 -0.34829912012015996 2216 76-77 -0.35434791884716077 2216 78-79 -0.41170869933451826 2216 80-81 -0.382755452493889 2216 82-83 -0.3677609661579808 2216 84-85 -0.44346709552153385 2216 86-87 -0.4117797107385499 2216 88-89 -0.5094802652715202 2216 90-91 -0.5050331714374536 2216 92-93 -0.48383145105379555 2216 94-95 -0.395326664469664 2216 96-97 -0.5202237384538293 2216 98-99 -0.6278586784536948 2216 100 -0.6223702264034756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33.0 3 6.0 4 9.0 5 36.0 6 73.0 7 215.0 8 491.0 9 989.0 10 1916.0 11 3031.0 12 4808.0 13 7706.0 14 9103.0 15 11904.0 16 14275.0 17 19394.0 18 21989.0 19 11882.0 20 8194.0 21 7619.0 22 7795.0 23 8769.0 24 10270.0 25 12608.0 26 15926.0 27 21392.0 28 27193.0 29 35885.0 30 46280.0 31 58647.0 32 75799.0 33 99119.0 34 135722.0 35 200607.0 36 346217.0 37 795375.0 38 1644761.0 39 930028.0 40 3316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.271893542801074 21.10872396272336 14.076840269175209 23.542542225300355 2 14.85598185944206 21.006187213556945 40.94303788088154 23.194793046119457 3 17.255263145306863 26.39828354473755 31.123536336173363 25.222916973782223 4 11.575483420684437 17.679363288184717 40.79993390233853 29.945219388792317 5 11.685253962601031 38.4840515391429 37.14626195107251 12.684432547183555 6 29.227700255251793 40.08512016837052 18.142075671068707 12.545103905308977 7 25.425981142683952 33.37985407604761 24.039294844394313 17.154869936874128 8 23.979864168146765 37.11785740764536 21.21624233616699 17.686036088040886 9 24.331681952053557 16.574900714922137 22.505240921497716 36.58817641152659 10-11 22.774471034640502 27.359286821894198 30.183323491375745 19.682918652089555 12-13 23.871478820415437 24.771991106631283 30.270175427916186 21.086354645037094 14-15 20.918778006553072 26.785885893067963 27.676333932690785 24.619002167688183 16-17 20.026440551862 30.262745510286237 28.18589788676699 21.524916051084773 18-19 19.764232363683725 28.93554297457537 30.93276693556646 20.36745772617445 20-21 21.000812826681152 28.168667895889914 30.508482499222993 20.32203677820594 22-23 20.540610526562965 27.981905798484753 30.628102867912027 20.849380807040262 24-25 20.50564329275949 28.150888736667973 30.52402757413912 20.81944039643342 26-27 20.38614828586418 28.0390827184488 30.616162246462725 20.958606749224295 28-29 20.3108098911786 28.13125968746596 30.68564158012041 20.87228884123503 30-31 20.6302224475668 28.57660473942552 30.27265002904742 20.52052278396026 32-33 20.274299895072858 28.253197929634894 30.751196573800566 20.72130560149168 34-35 20.411405271403854 28.070292921962125 30.73357464111483 20.784727165519193 36-37 20.799490018441606 28.49965495364377 30.04014452376428 20.66071050415034 38-39 20.74991814117636 28.58916697938984 30.059788467233204 20.601126412200596 40-41 20.567733474308962 28.33399266183145 30.240185816490563 20.85808804736903 42-43 20.574611980479116 28.19128743818191 30.173912495200444 21.060188086138528 44-45 20.75104872785083 28.393336322140673 29.996225579871382 20.85938937013712 46-47 20.829852793266575 28.655838980106456 29.637199084572668 20.8771091420543 48-49 20.87456532203674 28.5907650201701 29.406450692723503 21.128218965069657 50-51 20.919919241324163 28.500959476729697 29.277770361322457 21.301350920623683 52-53 20.896763956548945 28.53082218437173 29.324200512155763 21.248213346923563 54-55 21.01409493710242 28.893338278925302 29.127434946695015 20.96513183727727 56-57 20.901775499404053 28.55873897841058 29.188671434553598 21.350814087631772 58-59 20.65613380232388 28.29693206609062 29.617359462640852 21.429574668944653 60-61 20.986015077677827 28.649251138522523 29.176887242677385 21.187846541122262 62-63 20.81116550006066 28.64115222436406 29.176908984728815 21.370773290846465 64-65 20.78739056681963 28.345862552838618 29.398873587799407 21.467873292542347 66-67 20.735201026485843 28.412004961971515 29.216482694466638 21.636311317076 68-69 21.206188584006686 28.584130616092807 29.04419342466754 21.165487375232967 70-71 21.129054350803806 28.401219294774517 29.074853567219925 21.394872787201752 72-73 20.906331142271192 28.555980795185427 29.19198128873155 21.345706773811834 74-75 21.01357076279745 28.507440035848365 29.088095268637705 21.39089393271648 76-77 21.034730430602462 28.54716443939256 28.905516970053473 21.512588159951505 78-79 21.12505549658628 28.695594321150104 28.93269139201745 21.24665879024617 80-81 21.086702517860008 28.34222075922374 29.107747519123222 21.46332920379303 82-83 20.860081982869197 28.44350281902402 29.232870853880055 21.463544344226733 84-85 20.84628978414926 28.387889938256922 29.09359344364091 21.67222683395291 86-87 20.96364897605896 28.526796599919358 28.970999561569577 21.538554862452102 88-89 20.71364050607371 28.413936888744036 29.022041551843703 21.850381053338552 90-91 20.68364037532319 28.277758988643793 29.165961394689333 21.87263924134369 92-93 20.765127182018862 28.48532173981862 28.9676395788712 21.78191149929131 94-95 20.4705244667896 28.22129267199741 29.191547862016364 22.116634999196627 96-97 20.45097036094718 28.257885697848685 29.132005224192614 22.159138717011523 98-99 20.570673127253908 28.26791376350985 28.97380307391732 22.187610035318926 100 20.58222169848036 28.20341950288104 28.954955252683945 22.259403545954655 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 26.0 2 55.0 3 108.5 4 90.5 5 75.0 6 163.5 7 370.5 8 672.0 9 886.0 10 844.5 11 793.5 12 948.5 13 1156.5 14 1402.5 15 1749.0 16 2161.0 17 2537.0 18 3073.5 19 3812.5 20 4853.0 21 6429.5 22 8811.0 23 12534.5 24 17492.5 25 24172.0 26 33822.5 27 45718.5 28 59764.0 29 75956.0 30 92729.5 31 110869.5 32 128112.5 33 142427.0 34 155716.0 35 166749.5 36 174770.5 37 181229.0 38 185948.5 39 188190.5 40 187165.5 41 183030.5 42 177946.5 43 172462.5 44 165195.5 45 158015.0 46 151875.5 47 144511.0 48 138163.5 49 132815.0 50 125931.0 51 118006.5 52 109313.0 53 101014.5 54 94927.5 55 89165.0 56 81534.5 57 73557.5 58 65660.0 59 57094.5 60 49150.5 61 41346.5 62 33328.0 63 26492.5 64 20587.5 65 16123.0 66 12726.5 67 9638.5 68 7292.0 69 5550.0 70 3830.5 71 2506.0 72 1802.0 73 1376.0 74 1064.5 75 745.5 76 471.0 77 297.5 78 191.0 79 106.5 80 69.5 81 34.5 82 7.5 83 4.5 84 5.5 85 5.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04520172492739242 2 0.24372839655414577 3 0.002956918994769297 4 0.0027612405318801526 5 8.696820572850874E-5 6 4.348410286425437E-5 7 0.0 8 4.130989772104165E-4 9 0.0 10-11 5.652933372353068E-4 12-13 0.0 14-15 2.609046171855262E-4 16-17 1.6306538574095388E-4 18-19 5.000671829389253E-4 20-21 1.0871025716063593E-5 22-23 1.739364114570175E-4 24-25 0.001576298728829221 26-27 1.9567846288914468E-4 28-29 2.3916256575339906E-4 30-31 1.304523085927631E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.1958128287669953E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0871025716063593E-5 68-69 2.1742051432127187E-4 70-71 4.348410286425437E-5 72-73 3.152597457658442E-4 74-75 1.9567846288914468E-4 76-77 1.0871025716063593E-5 78-79 0.0 80-81 0.0 82-83 1.413233343088267E-4 84-85 0.0 86-87 4.4571205435860726E-4 88-89 0.0019893977060396377 90-91 0.0020111397574717645 92-93 6.305194915316884E-4 94-95 5.435512858031796E-4 96-97 2.3916256575339906E-4 98-99 1.413233343088267E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4599382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.27551535855937 #Duplication Level Percentage of deduplicated Percentage of total 1 67.32987588986653 36.543637129503466 2 16.924028723297 18.37120761800012 3 6.80336597944952 11.077685841225382 4 3.3294478467674677 7.228299909710005 5 1.8030884919774184 4.8931778569581015 6 1.0945442111165289 3.564417068464645 7 0.7091257372533072 2.6941715390409122 8 0.4757222490106003 2.0656056186066594 9 0.3362445669901549 1.6424862443915667 >10 1.1579182602853064 9.741399654563047 >50 0.027807849654544855 1.0233399231018143 >100 0.008308956436635309 0.7150469429770354 >500 3.608571267054594E-4 0.1285257389950158 >1k 1.2028557615290081E-4 0.18874071689386623 >5k 4.009519205096664E-5 0.12225819756828513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5618 0.12214684494569053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4939 0.10738399202327618 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.1742051432127186E-5 0.0 18-19 0.0 0.0 0.0 4.348410286425437E-5 0.0 20-21 0.0 0.0 0.0 4.348410286425437E-5 0.0 22-23 0.0 0.0 0.0 7.609718001244515E-5 0.0 24-25 0.0 0.0 0.0 8.696820572850874E-5 0.0 26-27 0.0 0.0 0.0 2.2829154003733547E-4 0.0 28-29 0.0 0.0 0.0 6.522615429638155E-4 0.0 30-31 0.0 0.0 0.0 0.002793853609028343 0.0 32-33 0.0 0.0 0.0 0.00792497774701036 0.0 34-35 0.0 0.0 0.0 0.014849821128142867 0.0 36-37 0.0 0.0 0.0 0.028395119170358106 0.0 38-39 0.0 0.0 0.0 0.05270273267147629 0.0 40-41 0.0 0.0 0.0 0.0809782705589577 0.0 42-43 0.0 0.0 0.0 0.11165630512968916 0.0 44-45 0.0 0.0 0.0 0.14601961741816616 0.0 46-47 0.0 0.0 0.0 0.1896341725910133 0.0 48-49 0.0 0.0 0.0 0.23959740678204158 0.0 50-51 0.0 0.0 0.0 0.2946265389567555 0.0 52-53 0.0 0.0 0.0 0.35029706164871716 0.0 54-55 0.0 0.0 0.0 0.4108051907843271 0.0 56-57 0.0 0.0 0.0 0.4836628051333853 0.0 58-59 0.0 0.0 0.0 0.5666630864755309 0.0 60-61 0.0 0.0 0.0 0.6551206227271402 0.0 62-63 0.0 0.0 0.0 0.7485462177309908 0.0 64-65 0.0 0.0 0.0 0.8502011792019015 0.0 66-67 1.0871025716063593E-5 0.0 0.0 0.9661950235923 0.0 68-69 2.1742051432127186E-5 0.0 0.0 1.0973322068051752 0.0 70-71 2.1742051432127186E-5 0.0 0.0 1.243993214740589 0.0 72-73 2.1742051432127186E-5 0.0 0.0 1.3987422658087543 0.0 74-75 2.1742051432127186E-5 0.0 0.0 1.5566547853602941 0.0 76-77 2.1742051432127186E-5 0.0 0.0 1.729797611940039 0.0 78-79 2.1742051432127186E-5 0.0 0.0 1.9280742499753227 0.0 80-81 2.1742051432127186E-5 0.0 0.0 2.151267278951824 0.0 82-83 2.1742051432127186E-5 0.0 0.0 2.3861901446759584 0.0 84-85 2.1742051432127186E-5 0.0 0.0 2.6183843829453606 0.0 86-87 2.1742051432127186E-5 0.0 0.0 2.8650370854171276 0.0 88 2.1742051432127186E-5 0.0 0.0 3.067825199124578 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5350 0.0 47.582264 1 GTATCAA 10170 0.0 43.155273 1 TCAACGC 11515 0.0 37.386314 4 ATCAACG 11615 0.0 37.106106 3 CAACGCA 11835 0.0 36.414364 5 AACGCAG 12290 0.0 35.142715 6 TATCAAC 13010 0.0 33.284325 2 ACGCAGA 14140 0.0 30.345413 7 CGCAGAG 14180 0.0 30.160381 8 GCAGAGT 16080 0.0 26.362839 9 GTACATG 8900 0.0 25.636604 1 TACATGG 9245 0.0 23.445118 2 AGAGTAC 15265 0.0 21.628124 10-11 ACATGGG 9585 0.0 21.428146 3 GAGTACT 11970 0.0 20.691204 12-13 GTGGTAT 3015 0.0 20.326433 1 CAGAGTA 16110 0.0 20.187376 10-11 AGTACTT 13145 0.0 19.234947 12-13 TATACCG 270 2.6024281E-8 19.146994 5 TAGGGCG 240 3.424846E-6 17.623938 5 >>END_MODULE