##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033071_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144913
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.1634773967829	31.0	30.0	34.0	15.0	34.0
2	29.159813129256865	33.0	30.0	34.0	12.0	34.0
3	29.407844706824093	33.0	30.0	34.0	16.0	34.0
4	32.333268926873366	37.0	35.0	37.0	17.0	37.0
5	32.868314091903414	37.0	35.0	37.0	20.0	37.0
6	32.65544154078654	37.0	35.0	37.0	17.0	37.0
7	32.897014070511275	37.0	35.0	37.0	20.0	37.0
8	32.48901064776797	37.0	35.0	37.0	13.0	37.0
9	33.67281058290146	39.0	35.0	39.0	13.0	39.0
10-11	33.966500590009176	39.0	35.0	39.0	14.0	39.0
12-13	34.00474077549978	39.0	35.0	39.0	14.5	39.0
14-15	35.23336760677096	40.0	36.0	41.0	15.0	41.0
16-17	35.404766998129915	40.0	36.0	41.0	18.0	41.0
18-19	35.384482413586085	40.0	35.5	41.0	21.0	41.0
20-21	35.385051720687585	40.0	35.0	41.0	23.5	41.0
22-23	35.30862310489742	40.0	35.0	41.0	24.0	41.0
24-25	35.19565187388295	40.0	35.0	41.0	24.5	41.0
26-27	34.80367875897953	40.0	35.0	41.0	22.0	41.0
28-29	34.528938052486666	40.0	35.0	41.0	16.0	41.0
30-31	34.394453913727546	40.0	35.0	41.0	2.0	41.0
32-33	34.14625671954897	40.0	35.0	41.0	2.0	41.0
34-35	33.83655020598566	40.0	34.5	41.0	2.0	41.0
36-37	33.50857410998323	40.0	34.0	41.0	2.0	41.0
38-39	33.24134480688413	40.0	34.0	41.0	2.0	41.0
40-41	33.077977821175466	40.0	33.5	41.0	2.0	41.0
42-43	32.85374328045103	40.0	33.0	41.0	2.0	41.0
44-45	32.680805034744985	40.0	33.0	41.0	2.0	41.0
46-47	32.468860626720854	39.5	33.0	41.0	2.0	41.0
48-49	32.27228406009122	39.0	33.0	41.0	2.0	41.0
50-51	32.07282645449338	39.0	33.0	41.0	2.0	41.0
52-53	31.926310958989184	39.0	32.0	41.0	2.0	41.0
54-55	31.736607481730417	38.5	32.0	41.0	2.0	41.0
56-57	31.496625561543823	38.0	31.0	41.0	2.0	41.0
58-59	31.287141250267403	38.0	31.0	40.0	2.0	41.0
60-61	30.969305721363853	37.0	31.0	40.0	2.0	41.0
62-63	30.752275503232973	37.0	31.0	40.0	2.0	41.0
64-65	30.469229813750317	36.0	30.0	40.0	2.0	41.0
66-67	30.111494482896635	35.5	30.0	39.0	2.0	41.0
68-69	29.747030977207015	35.0	29.5	39.0	2.0	41.0
70-71	29.33560481116256	35.0	29.0	38.5	2.0	40.5
72-73	28.97376011813985	35.0	28.5	37.0	2.0	40.0
74-75	28.652036049215738	35.0	28.0	37.0	2.0	39.0
76-77	28.298175456998337	35.0	28.0	36.0	2.0	39.0
78-79	27.910508373989913	35.0	27.0	36.0	2.0	38.5
80-81	27.50797720011317	34.0	26.0	35.0	2.0	37.0
82-83	27.314971741665687	34.0	25.5	35.0	2.0	37.0
84-85	27.149106705402552	34.0	26.0	35.0	2.0	36.5
86-87	27.04520643420535	34.0	26.0	35.0	2.0	36.0
88-89	26.789314968291322	34.0	25.0	35.0	2.0	36.0
90-91	26.49976882681333	34.0	24.5	35.0	2.0	35.5
92-93	26.195251633738863	34.0	23.5	35.0	2.0	35.0
94-95	25.940240006072607	34.0	20.0	35.0	2.0	35.0
96-97	25.720318397935316	34.0	18.5	35.0	2.0	35.0
98-99	25.48159930441023	34.0	8.5	35.0	2.0	35.0
100	25.309703063217242	34.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4717233328031156
1101	2	0.035688964943542345
1101	3	0.6489040924364033
1101	4	0.7826051467256221
1101	5	0.47065475454449057
1101	6	0.10270655302526421
1101	7	0.5281952606836029
1101	8	0.5555992976108115
1101	9	0.7485096959338193
1101	10-11	0.36041653650967476
1101	12-13	0.5371490094380249
1101	14-15	0.7913665549729174
1101	16-17	0.5650245680709389
1101	18-19	0.7816920511645904
1101	20-21	0.3526756815470975
1101	22-23	0.666328218149836
1101	24-25	1.0260164038038226
1101	26-27	0.8053542073545259
1101	28-29	0.8304881727348317
1101	30-31	0.8099262497209807
1101	32-33	0.8000808939052746
1101	34-35	0.7978153628166069
1101	36-37	0.7153871520000976
1101	38-39	1.1037738848046885
1101	40-41	1.0233897587851857
1101	42-43	1.0982179478109728
1101	44-45	0.7501936788151369
1101	46-47	0.6326012336002833
1101	48-49	0.7523929016611675
1101	50-51	0.8085381248381225
1101	52-53	0.902456291698293
1101	54-55	0.6852589123943602
1101	56-57	0.8580389590891464
1101	58-59	0.7346995814922721
1101	60-61	1.0401963784170825
1101	62-63	0.6360016040823915
1101	64-65	0.7094500957907819
1101	66-67	0.5989399668635116
1101	68-69	0.8239266166977259
1101	70-71	0.9305499880061383
1101	72-73	0.8902047814235488
1101	74-75	0.9101174233623013
1101	76-77	0.9508940774500694
1101	78-79	0.9093780306460104
1101	80-81	1.0340424992722852
1101	82-83	1.1374881367722232
1101	84-85	1.460124003944177
1101	86-87	1.3991304046597577
1101	88-89	1.2330082059930731
1101	90-91	1.5128968551619373
1101	92-93	1.2001943455200603
1101	94-95	1.2402807744104472
1101	96-97	1.5162252938596623
1101	98-99	1.556192666064586
1101	100	1.6434736055033348
1102	1	-0.7063893154182281
1102	2	-0.4373841575860986
1102	3	-0.42792397080470224
1102	4	-0.5368118493218077
1102	5	-0.6674381308310089
1102	6	-0.2977381110063604
1102	7	-0.3939885337828031
1102	8	0.07363289444875676
1102	9	-0.3547511736313993
1102	10-11	-0.8784668626998169
1102	12-13	-0.8642837968860846
1102	14-15	-0.17800103395988742
1102	16-17	-0.14532622244290394
1102	18-19	-0.12394035990260477
1102	20-21	-0.8584162156861055
1102	22-23	-0.8029158529964135
1102	24-25	-0.775366995405669
1102	26-27	-0.7018098242660358
1102	28-29	-0.7998230920873084
1102	30-31	-0.770730864903534
1102	32-33	-0.8296127819840535
1102	34-35	-0.6995907636656042
1102	36-37	-0.5824142313991061
1102	38-39	-0.8727577357486709
1102	40-41	-0.6150736799499938
1102	42-43	-0.2865399573273635
1102	44-45	-0.3898754911453324
1102	46-47	-0.5592218889293576
1102	48-49	-0.4060804184969271
1102	50-51	-0.12575041271128384
1102	52-53	-0.3034478584993323
1102	54-55	-0.26138140381117125
1102	56-57	-0.24954503300570963
1102	58-59	-0.07409488491088823
1102	60-61	-0.056271012887265215
1102	62-63	-0.3354657872219562
1102	64-65	-0.3563848844463742
1102	66-67	-0.3334958038874767
1102	68-69	-0.3777779287568208
1102	70-71	-0.38120890527449447
1102	72-73	-0.5967023332009553
1102	74-75	-0.32086083750726146
1102	76-77	-0.5090861597040792
1102	78-79	-0.38187691006545066
1102	80-81	-0.08626382483838668
1102	82-83	-0.31767490670603493
1102	84-85	-0.15585919763684686
1102	86-87	-0.4090464728105978
1102	88-89	-0.28453131969862966
1102	90-91	0.021666618007788685
1102	92-93	0.21575758662677913
1102	94-95	-0.025900055629083596
1102	96-97	-0.29812747293875574
1102	98-99	-0.027181839864269364
1102	100	-0.4321192798721576
1103	1	-0.483910129479284
1103	2	-0.12424817238721175
1103	3	-0.3282477450877721
1103	4	-0.4815389538546455
1103	5	-0.21080075802804998
1103	6	-0.6899675282126481
1103	7	-0.570329884384094
1103	8	-0.20515989001007995
1103	9	-0.7355328572576667
1103	10-11	-0.4969335602021303
1103	12-13	-0.006975748782480196
1103	14-15	-0.14371565005609455
1103	16-17	-0.17330957119405355
1103	18-19	-0.4999810629553352
1103	20-21	-0.9053634867314315
1103	22-23	-0.8603279714052903
1103	24-25	-0.8180358205675589
1103	26-27	-0.49638660502459686
1103	28-29	-0.19592623732319936
1103	30-31	-0.010612918557555417
1103	32-33	-0.04723535367693188
1103	34-35	-0.1318872484019593
1103	36-37	-0.11675743213915979
1103	38-39	-0.09448530281620293
1103	40-41	-0.5455778427622064
1103	42-43	-0.2591925937750901
1103	44-45	-0.18416318772257512
1103	46-47	-0.18092632926237684
1103	48-49	0.0875597295141688
1103	50-51	-0.25323653667057755
1103	52-53	-0.17642426923013943
1103	54-55	-0.12337261565206248
1103	56-57	-0.06719073143309018
1103	58-59	-0.8808710181208781
1103	60-61	-0.5110004300935564
1103	62-63	-0.31147180017292797
1103	64-65	-0.25954168370632047
1103	66-67	-0.17686999445177065
1103	68-69	-0.25410771599086246
1103	70-71	-0.18538328085266542
1103	72-73	-0.1996163017486019
1103	74-75	-0.40371236387174037
1103	76-77	-0.42918329198900196
1103	78-79	-0.24826220146230327
1103	80-81	-0.46039657229444586
1103	82-83	-0.6889746291852212
1103	84-85	-0.6609470792279666
1103	86-87	-0.5838845856690611
1103	88-89	-0.42040088491602035
1103	90-91	-0.6284837057664951
1103	92-93	-0.3155213217913513
1103	94-95	-0.44171874203056305
1103	96-97	-0.3628245127167382
1103	98-99	-0.5901443745543702
1103	100	-0.6643116943032403
1104	1	1.8451324237122044
1104	2	2.130007146761727
1104	3	2.09685863789095
1104	4	2.4160142376347125
1104	5	2.1945183909081223
1104	6	1.68247928029799
1104	7	2.0079679879563272
1104	8	2.2603720248835373
1104	9	2.563509695933817
1104	10-11	2.446098354691486
1104	12-13	2.5916944639834796
1104	14-15	2.680912009518373
1104	16-17	2.614456386252755
1104	18-19	2.494646596619141
1104	20-21	2.1825620451834595
1104	22-23	2.163714581786202
1104	24-25	2.4916982219856365
1104	26-27	2.715581480081795
1104	28-29	2.6008290818257365
1104	30-31	2.56481261335734
1104	32-33	2.56462634845073
1104	34-35	2.422474453725698
1104	36-37	2.338455333818281
1104	38-39	2.663546612077411
1104	40-41	2.9912306678760956
1104	42-43	3.1262861296291575
1104	44-45	3.2961027697242287
1104	46-47	3.0606694154184737
1104	48-49	3.052506538024808
1104	50-51	3.429901761201762
1104	52-53	3.6291608371528383
1104	54-55	3.4175316396670894
1104	56-57	3.323607140907331
1104	58-59	3.4974268542195475
1104	60-61	3.658946378417088
1104	62-63	3.4772516040823938
1104	64-65	3.5677455503362374
1104	66-67	3.485417239590781
1104	68-69	3.497222071243179
1104	70-71	3.3625954425515907
1104	72-73	3.38134114505991
1104	74-75	3.2601174233623027
1104	76-77	3.2238486229046153
1104	78-79	3.288014394282378
1104	80-81	3.421997044726833
1104	82-83	3.431238136772226
1104	84-85	3.395010367580543
1104	86-87	3.298562222841575
1104	88-89	2.9445991150839816
1104	90-91	2.839601400616484
1104	92-93	3.285648890974606
1104	94-95	3.073121683501352
1104	96-97	3.2186116574960266
1104	98-99	3.3010790297009507
1104	100	3.579837241866972
1105	1	-0.898382427772944
1105	2	-0.512072061159067
1105	3	-0.8050225502278607
1105	4	-0.191461009890034
1105	5	-0.18320438136910155
1105	6	-0.980045472177256
1105	7	-0.08054686352881646
1105	8	-0.6434893612550745
1105	9	-0.850500205056278
1105	10-11	-0.4352630314471284
1105	12-13	-0.46189464492741195
1105	14-15	-0.46015234691727613
1105	16-17	-0.5744049998858571
1105	18-19	-0.7768137994204665
1105	20-21	-1.0240963706581283
1105	22-23	-1.2327210617781574
1105	24-25	-1.4466433621727788
1105	26-27	-1.2610274308092926
1105	28-29	-1.2706313142138654
1105	30-31	-1.4214250104050379
1105	32-33	-1.4341112753116505
1105	34-35	-1.284852278947568
1105	36-37	-1.4391189236074595
1105	38-39	-1.3050920017839687
1105	40-41	-1.3054525004407367
1105	42-43	-1.4269564446282672
1105	44-45	-1.175605151067849
1105	46-47	-1.537993950918164
1105	48-49	-1.450042966925686
1105	50-51	-1.6213111100853652
1105	52-53	-1.476903519282807
1105	54-55	-1.6128396474616267
1105	56-57	-1.3422591957263315
1105	58-59	-1.2970285913250095
1105	60-61	-1.539939760196777
1105	62-63	-1.44042166324434
1105	64-65	-1.3629227664954477
1105	66-67	-1.2777758297161448
1105	68-69	-0.9972086218261254
1105	70-71	-1.0548798049731616
1105	72-73	-1.035144003454942
1105	74-75	-0.723545942974333
1105	76-77	-0.8774137533330055
1105	78-79	-0.5450549126483182
1105	80-81	-0.6779286978474239
1105	82-83	-1.0682668137228255
1105	84-85	-0.7984549789541084
1105	86-87	-1.0831209454752546
1105	88-89	-1.0998563304605753
1105	90-91	-1.0131213716607412
1105	92-93	-1.004351109025393
1105	94-95	-1.0384377224392374
1105	96-97	-0.8822051741871455
1105	98-99	-1.122559584160438
1105	100	-1.2090241442716412
1106	1	-1.0480327139024723
1106	2	-1.3209102844309335
1106	3	-1.0802973254118022
1106	4	-1.3105453953928077
1106	5	-0.8774999577157274
1106	6	-1.0949060408029254
1106	7	-0.9962521955299088
1106	8	-1.231462837501784
1106	9	-1.6368114049836109
1106	10-11	-1.3145209113635588
1106	12-13	-1.3322734259247824
1106	14-15	-1.0620008345183294
1106	16-17	-1.4949472834720154
1106	18-19	-1.4503763391606768
1106	20-21	-1.4491122667431426
1106	22-23	-1.1377533081220648
1106	24-25	-1.3519026954455597
1106	26-27	-1.1719185199182078
1106	28-29	-1.0198819273485782
1106	30-31	-1.116288304073855
1106	32-33	-1.0234699818245048
1106	34-35	-1.5083512343476997
1106	36-37	-1.8066364093009781
1106	38-39	-1.7973937548950616
1106	40-41	-1.3866133688211555
1106	42-43	-1.4943331364258867
1106	44-45	-1.6652045697253115
1106	46-47	-1.7346058139393286
1106	48-49	-1.5559108931678551
1106	50-51	-1.7586532846697978
1106	52-53	-1.5902198967921137
1106	54-55	-1.755472947488876
1106	56-57	-1.458709372854134
1106	58-59	-1.517802503578622
1106	60-61	-1.717544447270992
1106	62-63	-1.5426107812387109
1106	64-65	-1.565075550581195
1106	66-67	-1.860844228299122
1106	68-69	-1.9632137085733348
1106	70-71	-1.5615788693750154
1106	72-73	-1.6309065613621065
1106	74-75	-1.5394238610413709
1106	76-77	-1.6206238541596072
1106	78-79	-1.8267562479194552
1106	80-81	-2.0443561662823413
1106	82-83	-2.0669270008424583
1106	84-85	-1.8101272470983538
1106	86-87	-2.026667134956586
1106	88-89	-1.8275109766591413
1106	90-91	-2.0055820856220485
1106	92-93	-1.8529749622364022
1106	94-95	-2.036121435764702
1106	96-97	-1.9868241223204883
1106	98-99	-1.5208246400238181
1106	100	-1.3703921159311925
1107	1	-1.0554231318433516
1107	2	-0.6783261865716064
1107	3	-0.31721543618312253
1107	4	-0.3510227994023225
1107	5	-1.029555683165949
1107	6	-0.22326146044274964
1107	7	0.18833835832670331
1107	8	-0.39999834548683566
1107	9	-0.29315697073284497
1107	10-11	-0.38853127493814554
1107	12-13	-0.03608331379430041
1107	14-15	-0.027236138629781692
1107	16-17	-0.2961917618954004
1107	18-19	-0.4460015515290152
1107	20-21	-0.7524379548165427
1107	22-23	-0.5313780108063995
1107	24-25	-0.5585795557921429
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2216	80-81	-0.23322072530801208
2216	82-83	-0.5257305043427607
2216	84-85	-0.8200767404333931
2216	86-87	-0.6678140837786302
2216	88-89	-0.8990594215013843
2216	90-91	-0.661705219592573
2216	92-93	-0.6454660916037902
2216	94-95	-0.7013469576136266
2216	96-97	-0.9760834644551935
2216	98-99	-0.8653408309262289
2216	100	-0.9226888905372093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5556.0
3	803.0
4	633.0
5	768.0
6	1840.0
7	3546.0
8	3559.0
9	1972.0
10	1155.0
11	1137.0
12	1094.0
13	682.0
14	569.0
15	527.0
16	477.0
17	464.0
18	448.0
19	406.0
20	404.0
21	391.0
22	508.0
23	807.0
24	1513.0
25	1205.0
26	1251.0
27	1163.0
28	1264.0
29	1531.0
30	1791.0
31	2125.0
32	2832.0
33	3786.0
34	5626.0
35	9792.0
36	14784.0
37	25325.0
38	32979.0
39	10178.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92284161608629	22.241556701813987	12.548767772184588	19.286833909915135
2	15.801536730340416	29.050140487252595	31.859195184083173	23.289127598323816
3	21.017964898714425	31.656747538629364	26.82790427926373	20.49738328339248
4	12.89767901166364	23.465392344398484	32.73829665283235	30.898631991105525
5	12.12194464972684	44.979176307265156	28.8506564815902	14.048222561417806
6	27.073937217253167	40.878544048071284	14.790206167765998	17.257312566909555
7	23.222981233466182	40.91753638303898	18.743049751690506	17.116432631804336
8	22.121986599433583	39.00739103405402	17.920839953028942	20.949782413483454
9	20.922120531859782	26.703505439475045	19.129683992402	33.24469003626317
10-11	21.35554818744474	34.536637931034484	23.119750221043326	20.988063660477454
12-13	23.673866941138275	34.0501371206731	22.520257248053717	19.755738690134912
14-15	21.15676217121738	35.44410479595185	22.576032329931092	20.823100702899676
16-17	19.320332030440728	37.39969338287087	23.116790740715164	20.163183845973233
18-19	19.181554762751727	37.3764026123385	23.94758745497308	19.494455169936696
20-21	22.11743010277379	34.407462150513865	22.942106862636756	20.533000884075587
22-23	21.63728759726854	34.61275555647617	22.997493630507282	20.75246321574801
24-25	22.153735716479673	34.90718084463879	22.30351639477217	20.635567044109372
26-27	21.275036723407933	33.774475071286616	24.655318413548777	20.295169791756678
28-29	20.22544839372335	36.53437829066616	23.397262165754633	19.84291114985586
30-31	20.730734567453617	35.50219441365474	23.281848348923855	20.485222669967783
32-33	20.286181916120523	36.04932218255147	23.61484223393162	20.049653667396395
34-35	20.49337042124226	35.43666871432417	23.792974924248174	20.276985940185394
36-37	20.21976959750334	34.995701961217534	24.480178964120867	20.304349477158254
38-39	19.993715491313914	35.620733353821294	23.891492087389203	20.494059067475597
40-41	19.285408436697146	35.7651454460068	24.258266802950356	20.6911793143457
42-43	19.212720774848503	35.999723761675384	23.19642271369624	21.591132749779874
44-45	19.557133139741655	34.55162579027875	23.92054224085245	21.970698829127144
46-47	19.83342081272963	35.60837315947955	24.048316251830162	20.509889775960662
48-49	19.568310048824955	34.360130660276376	23.786109403810695	22.285449887087974
50-51	18.003521977832257	34.058906805704225	25.089948551500296	22.847622664963225
52-53	19.352354775330365	34.674019260850194	22.91309136990984	23.060534593909594
54-55	19.20779498794981	32.526879864098724	23.863173377713018	24.402151770238447
56-57	20.310611762918562	32.04659866999054	23.559329056494327	24.08346051059657
58-59	21.719089873286606	31.574077271000935	23.46511065842627	23.241722197286194
60-61	20.392837906890026	31.545802317391487	24.509729453520972	23.551630322197518
62-63	20.01353502359997	31.592195263432306	24.535337805875994	23.858931907091733
64-65	20.173254934727293	32.580421294681855	23.65250957252504	23.593814198065814
66-67	20.957227892330852	31.16342342591186	23.598201831314654	24.281146850442635
68-69	21.069931566917337	31.793761609800224	24.234702685532376	22.901604137750063
70-71	22.75264896889598	31.241088105620406	23.701409676116295	22.30485324936732
72-73	22.316666033716672	30.21657247219911	25.85784863748882	21.608912856595396
74-75	21.58866501871211	31.90725422230815	24.771104635907918	21.732976123071822
76-77	22.064489401367123	29.880549609887453	25.13636677483947	22.91859421390596
78-79	22.919155164914073	29.75737573619272	25.727562088750496	21.595907010142714
80-81	23.78644463315452	29.002782322928965	25.433055101040438	21.77771794287608
82-83	23.014226386304557	28.690627017360953	26.6610971681862	21.63404942814829
84-85	23.023100077322432	29.42118634706727	25.857105379432234	21.698608196178064
86-87	23.014418314054836	29.500070762613866	25.334051314976474	22.15145960835482
88-89	23.155227703428064	29.443867212984603	24.29987193515984	23.101033148427494
90-91	23.64293456124485	28.290404755165582	24.735421427240027	23.33123925634954
92-93	23.87634146930801	29.620884981411873	24.970227924653347	21.53254562462677
94-95	23.218951823073976	29.60963469484265	24.72670286465794	22.444710617425432
96-97	21.26933937188896	30.796633689341167	25.515889179617112	22.418137759152764
98-99	21.70094961287138	32.0317435113239	23.684746476490954	22.582560399313767
100	20.57678233612726	31.18449578149381	24.284372885568704	23.95434899681023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2932.0
1	1811.0
2	657.5
3	531.0
4	386.5
5	284.0
6	219.0
7	227.0
8	239.0
9	218.0
10	166.0
11	132.0
12	130.5
13	122.5
14	118.0
15	120.0
16	148.0
17	168.0
18	193.0
19	226.0
20	261.5
21	301.0
22	348.5
23	445.0
24	608.5
25	789.5
26	976.5
27	1160.5
28	1434.0
29	1956.0
30	2349.0
31	2621.5
32	2850.5
33	2930.0
34	3013.5
35	3205.0
36	3420.5
37	3326.5
38	3226.0
39	3412.0
40	3555.0
41	3547.0
42	3517.5
43	3650.5
44	3920.0
45	4064.5
46	4707.0
47	6418.5
48	9041.0
49	9238.0
50	6714.5
51	4929.0
52	4046.0
53	3530.5
54	2921.5
55	2526.0
56	2515.5
57	2536.0
58	2499.5
59	2401.0
60	2161.5
61	1829.0
62	1527.0
63	1269.0
64	1113.5
65	998.5
66	821.5
67	562.5
68	402.5
69	343.5
70	249.5
71	185.0
72	162.5
73	136.0
74	145.0
75	157.5
76	118.5
77	81.0
78	59.0
79	35.5
80	20.5
81	14.0
82	14.5
83	10.0
84	5.5
85	3.0
86	3.0
87	1.5
88	0.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06486650611056288
2	0.0414041528365295
3	0.05175519104566188
4	0.07176719824998447
5	0.08694872095671195
6	0.08832885938459627
7	0.09246927466824922
8	0.09867989759372867
9	0.09522955152401785
10-11	0.10006003602161297
12-13	0.10351038209132377
14-15	0.10730576276800563
16-17	0.07383740589181095
18-19	0.09557458613098893
20-21	0.08901892859853844
22-23	0.05658567554325699
24-25	0.023807387881004463
26-27	0.1728623380925107
28-29	0.06245126386176533
30-31	0.07832285578243499
32-33	0.07590761353363742
34-35	0.022082214846149067
36-37	0.05486050250840159
38-39	0.07763278656849282
40-41	0.08073809803123254
42-43	0.0762526481406085
44-45	0.07349237128483986
46-47	0.08073809803123254
48-49	0.07590761353363742
50-51	0.07452747510575311
52-53	0.07694271735455066
54-55	0.07038705982210015
56-57	0.07038705982210015
58-59	0.06762678296633153
60-61	0.06555657532450505
62-63	0.0714221636430134
64-65	0.06728174835936045
66-67	0.06831685218027368
68-69	0.06900692139421584
70-71	0.06383140228964965
72-73	0.06107112543388102
74-75	0.06003602161296778
76-77	0.05727574475719914
78-79	0.055205537115372676
80-81	0.04830484497595108
82-83	0.05279029486657512
84-85	0.04485449890624029
86-87	0.0431293258713849
88-89	0.04450946429926922
90-91	0.0414041528365295
92-93	0.0431293258713849
94-95	0.04243925665744274
96-97	0.045544568120182455
98-99	0.04450946429926922
100	0.05175519104566188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	144913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.92995797478487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54654135857923	40.716153830229175
2	7.926197222030268	7.915093883916557
3	2.9852809066408676	4.471648506345186
4	1.7497063091700644	3.4945104994030904
5	1.05175868979338	2.6257133590499127
6	0.8541220371778039	2.5587766452975234
7	0.624697671204478	2.183378992912989
8	0.5127496372054454	2.048125426980326
9	0.39665537972496717	1.782448779612595
>10	2.238960679980651	18.963792068344453
>50	0.07186787367839127	2.295170205571619
>100	0.030405638863934766	3.9340845886842453
>500	0.006910372469076083	2.0805586800356073
>1k	0.00414622348144565	4.930544533616722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3952	2.7271535334994104	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1948	1.3442548287593246	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1245	0.8591361713579873	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	889	0.6134715311945789	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	591	0.40783090543981565	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	526	0.3629764065335753	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	507	0.3498650914686743	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	502	0.34641474539896355	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	479	0.3305431534782939	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	456	0.3146715615576242	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	440	0.3036304541345497	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	413	0.2849985853581114	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	365	0.2518752630888878	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	339	0.2339334635263917	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	304	0.20978104103841613	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	292	0.20150021047111022	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	291	0.2008101412571681	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	291	0.2008101412571681	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	283	0.19528958754563083	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	252	0.1738974419134239	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	214	0.1476748117836219	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	203	0.14008405043025815	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	182	0.12559259693747282	No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169	0.11662169715622477	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	156	0.1076507973749767	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.10489052051920807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.900692139421584E-4	0.0
10-11	0.0	0.0	0.0	6.900692139421584E-4	0.0
12-13	0.0	0.0	0.0	6.900692139421584E-4	0.0
14-15	0.0	0.0	0.0	6.900692139421584E-4	0.0
16-17	0.0	0.0	0.0	0.0017251730348553961	0.0
18-19	0.0	0.0	0.0	0.0027602768557686338	0.0
20-21	0.0	0.0	0.0	0.0037953806766818712	0.0
22-23	0.0	0.0	0.0	0.007935795960334821	0.0
24-25	0.0	0.0	0.0	0.018631868776438278	0.0
26-27	0.0	0.0	0.0	0.035538564518021155	0.0
28-29	0.0	0.0	0.0	0.08729375556368305	0.0
30-31	0.0	0.0	0.0	0.1839034455155852	0.0
32-33	0.0	0.0	0.0	0.3367537764037733	0.0
34-35	0.0	0.0	0.0	0.6024304237715042	0.0
36-37	0.0	0.0	0.0	0.9667869687329639	0.0
38-39	0.0	0.0	0.0	1.42913334207421	0.0
40-41	0.0	0.0	0.0	1.8621517738229145	0.0
42-43	0.0	0.0	0.0	2.1809637506641915	0.0
44-45	0.0	0.0	0.0	2.5184075962819072	0.0
46-47	0.0	0.0	0.0	2.893805248666441	0.0
48-49	0.0	0.0	0.0	3.2340093711399254	0.0
50-51	0.0	0.0	0.0	3.59043012014105	0.0
52-53	0.0	0.0	0.0	3.9282190003657367	0.0
54-55	0.0	0.0	0.0	4.294300718362052	0.0
56-57	0.0	0.0	0.0	4.755611987882385	0.0
58-59	0.0	0.0	0.0	5.130664605659947	0.0
60-61	0.0	0.0	0.0	5.532974957388227	0.0
62-63	0.0	0.0	0.0	5.93079985922588	0.0
64-65	0.0	0.0	0.0	6.344841387591176	0.0
66-67	0.0	0.0	0.0	6.847901844555009	0.0
68-69	0.0	0.0	0.0	7.422384465161856	0.0
70-71	0.0	0.0	0.0	8.001352535659326	0.0
72-73	0.0	0.0	0.0	8.585841159868334	0.0
74-75	0.0	0.0	0.0	9.22657042501363	0.0
76-77	0.0	0.0	0.0	9.84349230227792	0.0
78-79	0.0	0.0	0.0	10.614644648858281	0.0
80-81	0.0	0.0	0.0	11.353363742383362	0.0
82-83	0.0	0.0	0.0	12.061374755888016	0.0
84-85	0.0	0.0	0.0	12.832527102468378	0.0
86-87	0.0	0.0	0.0	13.573316403635285	0.0
88	0.0	0.0	0.0	14.231297399129133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCAAC	20	0.002156416	70.52254	1
GCCGGAT	25	0.005234511	56.37908	94
CCTACAC	45	0.0010094291	41.791134	3
ACAACAC	330	0.0	38.46684	5
GAGAACA	210	0.0	38.059784	1
AGAGAAC	90	6.125247E-7	36.56724	3
AACAACA	465	0.0	34.37658	4
GTAACAA	55	0.0027049633	34.192745	1
TACATGA	125	6.168193E-9	33.85082	2
TAACAAC	70	2.1715376E-4	33.58216	1
CAACACA	525	0.0	33.12296	6
GTACAAA	130	9.042196E-9	32.548862	1
AACACAA	565	0.0	31.609806	7
GATTCTC	60	0.0041355975	31.354183	9
TGCGTCC	60	0.0041355975	31.354183	8
TATAGTG	60	0.004142601	31.343351	3
TAGTGCG	60	0.004142601	31.343351	5
AGTGCGT	60	0.0041496134	31.33253	6
GTGCTCA	65	0.00611674	28.942322	9
GTGCGTC	65	0.006137433	28.922333	7
>>END_MODULE