##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033071_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 144913 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48212375701283 33.0 31.0 34.0 31.0 34.0 2 32.423419568982766 34.0 31.0 34.0 31.0 34.0 3 32.48806525294487 34.0 31.0 34.0 30.0 34.0 4 36.07422384465162 37.0 35.0 37.0 35.0 37.0 5 36.091579085382264 37.0 35.0 37.0 35.0 37.0 6 36.21761332661666 37.0 37.0 37.0 35.0 37.0 7 36.17234478618205 37.0 37.0 37.0 35.0 37.0 8 36.19507566608931 37.0 37.0 37.0 35.0 37.0 9 37.931193198677825 39.0 38.0 39.0 35.0 39.0 10-11 37.90196531712131 39.0 38.0 39.0 35.0 39.0 12-13 37.87019453051141 39.0 38.0 39.0 35.0 39.0 14-15 39.13264510430396 41.0 38.5 41.0 36.0 41.0 16-17 39.160586006776484 40.5 38.5 41.0 36.0 41.0 18-19 39.30629412130037 41.0 39.0 41.0 36.0 41.0 20-21 39.309813474291474 41.0 39.0 41.0 36.0 41.0 22-23 39.136009191721925 41.0 39.0 41.0 35.0 41.0 24-25 38.977424385665884 41.0 38.5 41.0 35.0 41.0 26-27 38.73509968049795 40.0 38.0 41.0 35.0 41.0 28-29 38.58089336360437 40.0 38.0 41.0 35.0 41.0 30-31 38.25587766452975 40.0 37.5 41.0 35.0 41.0 32-33 37.796546893653435 40.0 35.5 41.0 34.0 41.0 34-35 37.41763678896993 40.0 35.0 41.0 33.0 41.0 36-37 37.06473884330598 40.0 35.0 41.0 33.0 41.0 38-39 36.72759172745026 40.0 35.0 41.0 32.5 41.0 40-41 36.459299717761695 40.0 35.0 41.0 31.5 41.0 42-43 36.04984714966911 40.0 35.0 41.0 30.5 41.0 44-45 35.42517579513225 40.0 35.0 41.0 26.0 41.0 46-47 34.528806939336015 40.0 35.0 41.0 10.0 41.0 48-49 33.71411467570197 40.0 35.0 41.0 2.0 41.0 50-51 33.24511258479225 39.0 35.0 41.0 2.0 41.0 52-53 33.08139366378448 39.0 34.5 41.0 2.0 41.0 54-55 32.85850475802723 39.0 34.0 41.0 2.0 41.0 56-57 32.61534161876436 39.0 34.0 41.0 2.0 41.0 58-59 32.419869162877035 38.0 34.0 41.0 2.0 41.0 60-61 32.09874545416905 37.5 33.0 41.0 2.0 41.0 62-63 31.88118043239737 37.0 33.0 40.0 2.0 41.0 64-65 31.557813998744074 36.5 33.0 40.0 2.0 41.0 66-67 31.129105049236436 36.0 32.5 39.0 2.0 41.0 68-69 30.63029886897656 35.5 32.0 39.0 2.0 41.0 70-71 30.0541773339866 35.0 31.0 38.5 2.0 40.5 72-73 29.61610759559184 35.0 31.0 37.0 2.0 40.0 74-75 29.312742818104656 35.0 31.0 37.0 2.0 39.0 76-77 28.988365433052934 35.0 30.0 36.5 2.0 39.0 78-79 28.65917826558004 35.0 30.0 36.0 2.0 38.5 80-81 28.350351590264502 35.0 29.0 35.5 2.0 37.0 82-83 28.087455921828962 35.0 29.0 35.0 2.0 37.0 84-85 27.932680297833873 35.0 29.0 35.0 2.0 36.5 86-87 27.83631558245292 35.0 29.0 35.0 2.0 36.0 88-89 27.6519946450629 35.0 29.0 35.0 2.0 36.0 90-91 27.456153002146117 35.0 29.0 35.0 2.0 35.5 92-93 27.276576290601945 35.0 28.0 35.0 2.0 35.0 94-95 27.099825412488872 35.0 27.0 35.0 2.0 35.0 96-97 26.962211809844526 35.0 27.0 35.0 2.0 35.0 98-99 26.845990352832388 35.0 27.0 35.0 2.0 35.0 100 26.725649182613015 35.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23788060910599995 1101 2 0.2938938293589075 1101 3 0.42611620424965935 1101 4 0.3293563100556014 1101 5 0.24130167616948484 1101 6 0.182725313827639 1101 7 0.2806233403135394 1101 8 0.16353066987107923 1101 9 0.4506121698185268 1101 10-11 0.4029434491934083 1101 12-13 0.44026379686773964 1101 14-15 0.6681130427488213 1101 16-17 0.6192409020581238 1101 18-19 0.6840720603336763 1101 20-21 0.4789280945406986 1101 22-23 0.6003519585490054 1101 24-25 0.5708471823249468 1101 26-27 0.7097007654838237 1101 28-29 0.704685028533639 1101 30-31 0.6913506178733755 1101 32-33 0.8275802049346836 1101 34-35 0.9201341488142489 1101 36-37 0.7497958029502598 1101 38-39 0.5970349279065488 1101 40-41 0.002755680014061568 1101 42-43 0.2664983204569893 1101 44-45 0.5124238856953269 1101 46-47 0.529716195749586 1101 48-49 0.009369420038019882 1101 50-51 0.23832701584063898 1101 52-53 0.32251990464967406 1101 54-55 0.24049008156228524 1101 56-57 0.25536950148207893 1101 58-59 0.4968332037908354 1101 60-61 0.38030458105004783 1101 62-63 0.24334995138124782 1101 64-65 0.14487614621312517 1101 66-67 0.35041777864249823 1101 68-69 0.533849510675978 1101 70-71 0.5031033961771918 1101 72-73 0.30711830979522503 1101 74-75 0.20706181108871036 1101 76-77 0.29197148165258113 1101 78-79 0.506005186789551 1101 80-81 0.4835001392611282 1101 82-83 0.4240312420466701 1101 84-85 0.5350440083682031 1101 86-87 0.6475651416618433 1101 88-89 0.9037547504641701 1101 90-91 0.8786107240047123 1101 92-93 0.8668494141305345 1101 94-95 1.0051758581833283 1101 96-97 0.8745383039438543 1101 98-99 0.8782516444865962 1101 100 0.6889949131504878 1102 1 0.009372703967656548 1102 2 -0.2940508346727171 1102 3 -0.13836008033532465 1102 4 -0.0051298559523047516 1102 5 -0.023767493790984418 1102 6 -0.06480433044113454 1102 7 0.17168065256650067 1102 8 0.0817618951675172 1102 9 0.2902860828620035 1102 10-11 -0.19611781562873176 1102 12-13 0.06884581267800627 1102 14-15 -0.037050000729443866 1102 16-17 -0.09740929557033695 1102 18-19 0.015593799464113545 1102 20-21 -0.029471114945465615 1102 22-23 0.0765130257426847 1102 24-25 -0.035254596331178334 1102 26-27 0.1625169710174177 1102 28-29 -0.1283189240355327 1102 30-31 0.009285400482070827 1102 32-33 -0.20342275949219868 1102 34-35 -0.056273953952548084 1102 36-37 -0.6258217859825521 1102 38-39 -0.5342644792080762 1102 40-41 -0.30171566385945425 1102 42-43 -0.3391093870528934 1102 44-45 -0.2540039799173144 1102 46-47 -0.6869340018788748 1102 48-49 -0.796460619487668 1102 50-51 -0.627656185740392 1102 52-53 -0.5996638898167177 1102 54-55 -1.004939760334949 1102 56-57 -1.0531779293479602 1102 58-59 -0.9782162033237825 1102 60-61 -0.9664098458274175 1102 62-63 -1.251091748223498 1102 64-65 -1.14909618580268 1102 66-67 -1.0638608774839824 1102 68-69 -0.7737097778615727 1102 70-71 -0.8334825722022536 1102 72-73 -0.7489587653035876 1102 74-75 -0.5466437225081258 1102 76-77 -0.5578308898889226 1102 78-79 -0.7378189238823865 1102 80-81 -0.7170927461143677 1102 82-83 -0.9992642124987832 1102 84-85 -0.9359293117899057 1102 86-87 -0.9871927634765001 1102 88-89 -0.9896493997334588 1102 90-91 -1.0131876949676197 1102 92-93 -1.0166733131421921 1102 94-95 -0.8510494382593592 1102 96-97 -0.9910743442774894 1102 98-99 -0.9437592250786224 1102 100 -0.9525564702487195 1103 1 0.2481562377326938 1103 2 0.3243580459933355 1103 3 0.2782680417738348 1103 4 0.30638725783122567 1103 5 0.29364693729134217 1103 6 0.22358604883730493 1103 7 0.008984075323205332 1103 8 -0.05225753128946309 1103 9 0.1460908932227838 1103 10-11 0.09128967743325234 1103 12-13 0.09844803284453008 1103 14-15 -0.10116887214479675 1103 16-17 0.3596906119227228 1103 18-19 0.2811465284187875 1103 20-21 0.3509058508269618 1103 22-23 0.3126948018758924 1103 24-25 0.41641052855319316 1103 26-27 0.3146330672246336 1103 28-29 0.24373241731507278 1103 30-31 0.09294636255422262 1103 32-33 0.14908649120160788 1103 34-35 0.02923714688001411 1103 36-37 0.4496870021765602 1103 38-39 0.4375209046957167 1103 40-41 0.2964573241146411 1103 42-43 0.7045423243254589 1103 44-45 0.41782765745549 1103 46-47 0.14782548008227536 1103 48-49 0.03644872371307173 1103 50-51 0.47230912415784587 1103 52-53 0.5192075255394286 1103 54-55 0.5610292498408143 1103 56-57 0.442023273242242 1103 58-59 0.15713301036723948 1103 60-61 0.21837276286823482 1103 62-63 0.2767759668551264 1103 64-65 -0.16701940504412605 1103 66-67 0.06270017709510967 1103 68-69 -0.06528008313446776 1103 70-71 0.019604846854175406 1103 72-73 0.21814345486292197 1103 74-75 0.09523879368058275 1103 76-77 -0.11917455316366699 1103 78-79 -0.05141744377137769 1103 80-81 -0.32827452224757536 1103 82-83 -0.218679106115804 1103 84-85 -0.06462958931071938 1103 86-87 -0.3667602935412546 1103 88-89 -0.4849662359961755 1103 90-91 -0.3202529123589244 1103 92-93 -0.3012477812272998 1103 94-95 -0.09359106638147097 1103 96-97 -0.3124781370619516 1103 98-99 -0.13571112147084818 1103 100 -0.026793288010050986 1104 1 -0.022801209075822726 1104 2 0.10116655663163243 1104 3 0.0031616587951148745 1104 4 -0.07382550812621957 1104 5 0.01471076707857577 1104 6 0.13954349564581747 1104 7 0.194941522131721 1104 8 0.15284885168925655 1104 9 0.20311216981852454 1104 10-11 0.18453435828431708 1104 12-13 0.17765016050410054 1104 14-15 0.3593630427488179 1104 16-17 0.3112863566035742 1104 18-19 0.3090720603336763 1104 20-21 0.33574627635887566 1104 22-23 0.28955650400354926 1104 24-25 0.33050627323404314 1104 26-27 0.49447349275655483 1104 28-29 0.4380941194427308 1104 30-31 0.5738506178733758 1104 32-33 0.8395120231164981 1104 34-35 0.6427477851778889 1104 36-37 0.6380912574957165 1104 38-39 0.9571485642701845 1104 40-41 1.199914770923158 1104 42-43 0.9875210477297216 1104 44-45 0.8245829766044181 1104 46-47 0.9692616502950386 1104 48-49 1.4602785109471128 1104 50-51 2.068213379477001 1104 52-53 2.0422926319224075 1104 54-55 2.123103717925929 1104 56-57 1.9462785923911738 1104 58-59 2.0831968401544785 1104 60-61 2.090872762868237 1104 62-63 1.8886908604721526 1104 64-65 2.0160125098494923 1104 66-67 2.417008687733407 1104 68-69 2.3949858743123436 1104 70-71 2.3016261234499176 1104 72-73 2.644845582522496 1104 74-75 2.6468345383614356 1104 76-77 2.5665169361980347 1104 78-79 2.3830506413350037 1104 80-81 2.2998637756247646 1104 82-83 2.2532357875012146 1104 84-85 2.4201576447318374 1104 86-87 2.5756333234800266 1104 88-89 2.3429592959187175 1104 90-91 2.0272470876410758 1104 92-93 2.180826686857806 1104 94-95 2.4769940400015074 1104 96-97 2.242947394852944 1104 98-99 2.577001644486593 1104 100 2.648313094968671 1105 1 -0.06730615957086883 1105 2 -0.08279383940796947 1105 3 -0.027135370907856782 1105 4 -0.16828095367077367 1105 5 -0.3033090349016163 1105 6 0.02667220851710539 1105 7 -0.09634560658115277 1105 8 0.11453202000608798 1105 9 0.126973555957143 1105 10-11 -0.09143098825033746 1105 12-13 -0.11039439395134565 1105 14-15 -0.009795373092764237 1105 16-17 -0.14465423745583195 1105 18-19 0.0473146345911033 1105 20-21 -0.05769431770053046 1105 22-23 0.06443274162731427 1105 24-25 -0.15352838023130033 1105 26-27 -0.392234428035529 1105 28-29 -0.15180687065628007 1105 30-31 -0.15518403559196514 1105 32-33 -0.20479490757657715 1105 34-35 -0.4827967692775559 1105 36-37 0.025814029772945446 1105 38-39 0.09756935634939623 1105 40-41 0.33592962240830104 1105 42-43 0.35484778040298437 1105 44-45 0.4198552538321394 1105 46-47 0.5294349176217708 1105 48-49 -0.5680383207360578 1105 50-51 -0.5901777096319094 1105 52-53 -0.6686479621369941 1105 54-55 -0.9699903414800133 1105 56-57 -0.8208006155296204 1105 58-59 -0.6440308826178018 1105 60-61 -1.0143005044584967 1105 62-63 -0.8385863672506204 1105 64-65 -0.8330716485663494 1105 66-67 -0.6724220053358962 1105 68-69 -0.8252864029153812 1105 70-71 -1.0502550646688924 1105 72-73 -0.8149563976755196 1105 74-75 -0.9487100160940081 1105 76-77 -0.7825672222178071 1105 78-79 -0.7430384675758859 1105 80-81 -1.0626857293257288 1105 82-83 -0.819189955073039 1105 84-85 -0.7336047315057854 1105 86-87 -0.784787468599184 1105 88-89 -0.9613723872496003 1105 90-91 -0.9229756846361497 1105 92-93 -0.8409307388847651 1105 94-95 -0.9373871481173026 1105 96-97 -0.99680508039458 1105 98-99 -1.0459439000678614 1105 100 -0.8971324495857829 1106 1 0.09687769000674962 1106 2 -0.08433803052432864 1106 3 0.057840557877682386 1106 4 0.02837632673616497 1106 5 0.19746306065655972 1106 6 0.21660771582930494 1106 7 0.2474185863519054 1106 8 0.2828488516892591 1106 9 0.3800846468827501 1106 10-11 0.3739609637888961 1106 12-13 0.314507958669239 1106 14-15 0.3931703821983632 1106 16-17 0.34614874192467937 1106 18-19 0.3646913263887228 1106 20-21 0.2496224231478692 1106 22-23 0.15297393519620783 1106 24-25 0.21594205305055425 1106 26-27 0.1777991808299504 1106 28-29 0.15855283503906747 1106 30-31 -0.20543837295231526 1106 32-33 -0.532276967709187 1106 34-35 -0.8767017561065202 1106 36-37 -0.5344775498437357 1106 38-39 -0.9452138210509133 1106 40-41 -0.6290989905447333 1106 42-43 -0.699176199976705 1106 44-45 -0.5942014270653075 1106 46-47 -1.2628713772278957 1106 48-49 -1.530455434007017 1106 50-51 -1.6517636847431874 1106 52-53 -1.6067440653253016 1106 54-55 -1.6968274747346257 1106 56-57 -1.7397305819207531 1106 58-59 -1.7521242607629546 1106 60-61 -1.5545630169482791 1106 62-63 -1.456217396408583 1106 64-65 -1.3836893250128917 1106 66-67 -1.1751243397895266 1106 68-69 -1.0658168779812378 1106 70-71 -1.2039702068253106 1106 72-73 -1.2079984541747493 1106 74-75 -0.971055369895442 1106 76-77 -1.1198821463707738 1106 78-79 -0.8450686247200423 1106 80-81 -1.1731408115311979 1106 82-83 -1.2544247629574983 1106 84-85 -0.8047964837085289 1106 86-87 -0.9300776856942861 1106 88-89 -0.745458135273946 1106 90-91 -0.9934180499736058 1106 92-93 -1.0212604691054956 1106 94-95 -0.9695426572462011 1106 96-97 -0.9078324216608173 1106 98-99 -0.7372873463390945 1106 100 -0.5701272720038055 1107 1 -0.2400234312980416 1107 2 -0.14550011003503016 1107 3 0.05890239953585308 1107 4 -0.12308476738547824 1107 5 -0.2812151588473455 1107 6 -0.1354565043541811 1107 7 0.020311892502093087 1107 8 -0.11529929645888615 1107 9 -0.0748507931444351 1107 10-11 -0.08611378986383755 1107 12-13 0.04116867902261845 1107 14-15 0.19473341311918801 1107 16-17 0.20804561586283654 1107 18-19 -0.21639090262928562 1107 20-21 -0.15406853845593815 1107 22-23 -0.01738794044089076 1107 24-25 -0.15190113417336448 1107 26-27 -0.03293391465085449 1107 28-29 -0.3502392138905961 1107 30-31 -0.1581864191636626 1107 32-33 0.3899749860794586 1107 34-35 -0.007159622229515605 1107 36-37 0.10596162786607977 1107 38-39 0.10261152723315092 1107 40-41 -0.11814078463240207 1107 42-43 -0.31525673004806265 1107 44-45 -0.749676282654832 1107 46-47 -0.46018279414940366 1107 48-49 0.012315547984151465 1107 50-51 0.01052819429180829 1107 52-53 0.03590374303352206 1107 54-55 0.1256963105185207 1107 56-57 -0.2092769631643847 1107 58-59 -0.010321678364043407 1107 60-61 -0.02699760750213187 1107 62-63 0.06795011973141385 1107 64-65 -0.14195045311346988 1107 66-67 -0.4048431641184429 1107 68-69 -0.6418659775395135 1107 70-71 -0.8055035061797113 1107 72-73 -0.5382099730330587 1107 74-75 -0.7925173134904142 1107 76-77 -0.8530201008390037 1107 78-79 -0.8612086179242553 1107 80-81 -0.9565251132641244 1107 82-83 -1.2631531013876725 1107 84-85 -1.079749762675572 1107 86-87 -0.8291814913347935 1107 88-89 -0.9246332966738784 1107 90-91 -1.0818269864330006 1107 92-93 -0.8222288686977492 1107 94-95 -0.8940244785170108 1107 96-97 -0.5877933458877962 1107 98-99 -0.7803131703282205 1107 100 -0.9324276457720693 1108 1 -0.18008191083020364 1108 2 0.23607883733338753 1108 3 0.21509148335651673 1108 4 -0.0041763853191980616 1108 5 0.10962304778033172 1108 6 0.037789109680907984 1108 7 0.02055555721943847 1108 8 0.02846288677697828 1108 9 -0.17987028632182245 1108 10-11 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-0.3546308613937086 2215 14-15 -0.29060046090081215 2215 16-17 -0.23506400835992736 2215 18-19 -0.19777100535975478 2215 20-21 -0.2703668623272506 2215 22-23 -0.3147318171643292 2215 24-25 -0.17925650048858444 2215 26-27 -0.21266154373979873 2215 28-29 -0.16380369077624124 2215 30-31 -0.2885763894258915 2215 32-33 -0.23585294038715432 2215 34-35 -0.07192374766882637 2215 36-37 -0.22380655272326067 2215 38-39 -0.07385508536484764 2215 40-41 0.00858265413482684 2215 42-43 0.20065973386110159 2215 44-45 0.25912677222486025 2215 46-47 0.6963966867913882 2215 48-49 1.1294755912390784 2215 50-51 1.3371367371412362 2215 52-53 1.1570006611194898 2215 54-55 0.9833774405536673 2215 56-57 1.4147092493254831 2215 58-59 1.531007059132584 2215 60-61 1.1928253176127654 2215 62-63 1.2201872108371177 2215 64-65 1.289534407659712 2215 66-67 1.4611510234998306 2215 68-69 1.439456677232048 2215 70-71 1.515275758486414 2215 72-73 1.4475463124495072 2215 74-75 1.088075414273849 2215 76-77 1.1794548924024149 2215 78-79 1.2648389625028855 2215 80-81 1.2243345785444717 2215 82-83 1.3104985612238416 2215 84-85 1.1748291775785518 2215 86-87 1.0727683599763722 2215 88-89 1.043415500298277 2215 90-91 0.9197105912907091 2215 92-93 1.191355883938101 2215 94-95 1.0733991494905624 2215 96-97 1.0826919204003893 2215 98-99 1.2726403306179819 2215 100 1.310137912486919 2216 1 -0.05090225437199081 2216 2 -0.029356091451987254 2216 3 -0.11781395096098635 2216 4 -0.01974885307395624 2216 5 -0.154069720726298 2216 6 -0.18728577264686663 2216 7 -0.11356022002856037 2216 8 -0.0812626465685824 2216 9 -0.18897842251596586 2216 10-11 -0.24632801105366298 2216 12-13 -0.29812510081994503 2216 14-15 -0.1460202325125053 2216 16-17 -0.10630946221175464 2216 18-19 -0.2146648734642298 2216 20-21 -0.3146457097038393 2216 22-23 -0.48714210226822274 2216 24-25 -0.3496766535952318 2216 26-27 -0.22417633302742246 2216 28-29 -0.4554947307314805 2216 30-31 -0.5630047828234908 2216 32-33 -0.15029633925284003 2216 34-35 -0.12958670959563534 2216 36-37 -0.032919195465957785 2216 38-39 0.015955184479246043 2216 40-41 -0.034710664616909526 2216 42-43 0.5744548456391243 2216 44-45 0.5010899452455391 2216 46-47 0.33508917642744507 2216 48-49 0.007944016173595969 2216 50-51 -0.1697047389898998 2216 52-53 -0.05190597434936706 2216 54-55 0.022868526288291946 2216 56-57 -0.029592487748203666 2216 58-59 -0.0924129159430791 2216 60-61 -0.16141817789831947 2216 62-63 -0.47397464475432827 2216 64-65 -0.39828191523761447 2216 66-67 -0.414271800071468 2216 68-69 -0.2810855542590893 2216 70-71 -0.1721717859577474 2216 72-73 -0.3735516300210584 2216 74-75 -0.21222469508803954 2216 76-77 -0.27112243662426394 2216 78-79 -0.28923158863015175 2216 80-81 0.009018827889573089 2216 82-83 -0.31350637277752824 2216 84-85 -0.41297824376990633 2216 86-87 -0.3556123211192812 2216 88-89 -0.6854814706318066 2216 90-91 -0.6882842712439441 2216 92-93 -0.5741646023408009 2216 94-95 -0.4695826150507578 2216 96-97 -0.5060247305825953 2216 98-99 -0.4894530593461539 2216 100 -0.8232200060766246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 1.0 5 24.0 6 57.0 7 177.0 8 166.0 9 247.0 10 347.0 11 605.0 12 925.0 13 1498.0 14 1597.0 15 1985.0 16 2677.0 17 3747.0 18 4331.0 19 1845.0 20 785.0 21 702.0 22 702.0 23 825.0 24 926.0 25 1203.0 26 1599.0 27 1926.0 28 1407.0 29 1589.0 30 1817.0 31 2110.0 32 2706.0 33 3464.0 34 4878.0 35 8472.0 36 13850.0 37 26636.0 38 37272.0 39 11780.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.48672774091998 18.887684586229987 14.260861160779006 19.364726512071027 2 16.844085686815657 22.96486895893424 36.728849785229606 23.462195569020494 3 21.341917853593905 26.368416694269627 32.6315280998123 19.65813735232417 4 12.392000441659535 18.61870980208132 36.98277527810749 32.00651447815165 5 11.395112929826862 37.88825019149421 34.56004637265118 16.156590506027754 6 27.45233346904695 37.382429457674604 16.116566491619107 19.04867058165934 7 21.953171903141886 36.539854947451225 22.46727346752879 19.03969968187809 8 25.560163684417546 29.678496753224348 21.253441720204535 23.507897842153568 9 24.18968622552842 15.538978559549523 23.429920020978106 36.84141519394395 10-11 24.072029424551282 25.515999254725248 27.211154278774163 23.200817041949307 12-13 28.149303375128525 24.040976309923888 28.575766149344776 19.23395416560281 14-15 23.56801518157509 24.21357715863021 31.433796256361596 20.784611403433107 16-17 18.386267575260934 27.458293797981543 31.999654964202534 22.155783662554988 18-19 17.813791723309848 24.132755515378193 37.8799693609269 20.17348340038506 20-21 20.047545768840614 21.30830222271294 38.48757530380297 20.156576704643474 22-23 19.41067885793151 22.186491848529286 39.06667816785991 19.336151125679287 24-25 20.417155475812574 22.580567248637085 38.48630184252295 18.515975433027396 26-27 19.715277442327466 21.238605232104778 40.79620185904646 18.24991546652129 28-29 18.55699626672555 23.10317224817649 39.73211513114766 18.607716353950302 30-31 19.644200313291424 21.89106567388709 39.18627038291941 19.278463629902078 32-33 19.386459461884026 22.182964951384623 39.52854471303472 18.90203087369663 34-35 19.364032212430907 21.66679317935589 40.00469247065481 18.9644821375584 36-37 19.793255263502928 21.37592900567927 39.917398715091124 18.913417015726676 38-39 19.273978180011454 21.68991049802295 39.33429022931 19.701821092655596 40-41 18.785754211147378 22.196076266449523 39.058262543733136 19.959906978669963 42-43 19.84156010847888 22.201251785554092 37.13124426379966 20.82594384216737 44-45 19.788079744398363 21.527744232746546 37.25235141084651 21.431824612008583 46-47 20.838710122625297 22.89097596488928 35.673818083953826 20.5964958285316 48-49 21.000531353294736 22.443466079647788 34.36579188892646 22.190210678131017 50-51 19.125613299013892 23.072809202763036 35.254946071090934 22.54663142713214 52-53 20.29528061664585 24.296991988296426 33.32033702980409 22.087390365253633 54-55 19.862262184897144 23.013463250364012 32.94631951584744 24.177955048891402 56-57 20.89667593659644 22.746751499175367 31.781137648106107 24.575434916122088 58-59 21.338320233519422 23.07625954883275 32.286613347318735 23.298806870329095 60-61 20.48090923519629 23.973694561564525 32.376322345131214 23.169073858107968 62-63 19.118712606874467 24.82869031763886 32.438083539779036 23.614513535707633 64-65 19.076273350217026 25.75096782207255 31.644158909138586 23.52859991857183 66-67 19.523783235458517 24.45915825357283 31.088308157308177 24.928750353660472 68-69 19.485484394084725 24.834210871350397 31.410915514826137 24.26938921973874 70-71 21.243780751209346 24.38773608992982 30.716705885600327 23.651777273260507 72-73 20.7241586331109 23.08074499872337 32.644759269354715 23.55033709881101 74-75 19.793945332716874 24.999137413482572 31.26151553000766 23.945401723792898 76-77 20.088259852463203 23.55309737566678 31.23874324594757 25.119899525922452 78-79 20.56026719479964 23.888816048249637 31.509595412419866 24.041321344530857 80-81 20.889430209850048 23.351252130588698 31.096934022482454 24.6623836370788 82-83 20.18555961162905 23.083505275579142 32.263841063258646 24.467094049533166 84-85 20.080669091109836 24.197276986881786 31.09451878023366 24.62753514177472 86-87 20.290450132148255 24.516088963723064 30.281962280816764 24.911498623311918 88-89 20.126630322268994 24.52522255192878 29.35925747015389 25.988889655648332 90-91 20.765647643364847 23.18887585397833 29.671520253950728 26.373956248706094 92-93 21.152837584189026 24.34442972286629 29.758750138014793 24.74398255492989 94-95 20.70690690276235 23.75494262074486 29.89034800190459 25.6478024745882 96-97 19.394050223237393 23.888816048249637 30.750519277083495 25.966614451429475 98-99 20.734854705927006 24.142761518980354 28.830401689289438 26.291982085803205 100 20.145880631827374 22.794366274937374 29.332772077039326 27.72698101619592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 208.0 1 149.5 2 261.0 3 460.5 4 296.5 5 139.5 6 301.5 7 590.5 8 924.5 9 1060.0 10 708.5 11 324.0 12 230.0 13 213.5 14 227.5 15 230.0 16 209.0 17 200.5 18 236.0 19 251.5 20 262.5 21 299.5 22 361.5 23 445.5 24 547.0 25 679.5 26 853.0 27 1071.5 28 1383.0 29 1929.0 30 2385.5 31 2695.0 32 2999.5 33 3193.5 34 3371.5 35 3506.0 36 3633.0 37 3517.0 38 3311.5 39 3457.0 40 3564.0 41 3566.5 42 3569.0 43 3632.0 44 4011.5 45 4237.0 46 4749.0 47 6508.5 48 9170.0 49 9346.5 50 7175.0 51 5296.0 52 3875.5 53 3126.0 54 2610.5 55 2339.5 56 2336.0 57 2375.0 58 2357.5 59 2286.5 60 2097.0 61 1823.5 62 1507.0 63 1204.0 64 1061.5 65 982.0 66 785.0 67 553.5 68 387.5 69 305.0 70 200.5 71 134.5 72 123.0 73 101.0 74 107.5 75 125.0 76 93.0 77 60.5 78 44.5 79 25.5 80 17.5 81 10.0 82 6.0 83 5.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04278429126441382 2 0.23462353274033387 3 6.900692139421583E-4 4 0.0034503460697107922 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.4503460697107917E-4 16-17 3.4503460697107917E-4 18-19 0.0 20-21 0.0 22-23 3.4503460697107917E-4 24-25 0.0020702076418264753 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0020702076418264753 90-91 0.0020702076418264753 92-93 6.900692139421583E-4 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 144913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.843637216812844 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47662919176625 33.622242310903786 2 9.051960184260542 7.756377964709861 3 3.427503785072319 4.405401861806739 4 1.9843442966208162 3.4006610863069566 5 1.3948394162935283 2.987999696369546 6 1.0018361627420032 2.575338306432135 7 0.7215797442257514 2.164057054922609 8 0.6378249524852624 2.186139269768758 9 0.4815900525078118 1.8569762547183482 >10 2.646329285185066 19.84570052376253 >50 0.10308282060367877 2.9555664433142645 >100 0.04670940308604194 4.916053080123937 >500 0.016106690719324808 4.1514563910760245 >1k 0.009664014431594885 7.176029755784505 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 1.6734178438097342 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2306 1.5912996073506172 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1950 1.3456349671872088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.8977800473387482 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1215 0.8384340949397224 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1202 0.8294631951584743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 860 0.5934595239902563 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.46855699626672553 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.4513052659181716 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.40507062858404697 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.400930213300394 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 580 0.40024014408645187 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 547 0.3774678600263606 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 516 0.35607571439415375 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 511 0.3526253683244429 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 501 0.3457246761850214 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 492 0.3395140532595419 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 482 0.33261336112012035 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 479 0.3305431534782939 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 403 0.27809789321868983 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 385 0.26567664736773094 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 373 0.2573958168004251 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 320 0.2208221484614907 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 318 0.21944201003360636 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 300 0.20702076418264753 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 280 0.19321937990380433 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 252 0.1738974419134239 RNA PCR Primer, Index 1 (95% over 23bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 249 0.17182723427159743 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.15043508863939054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 211 0.14560460414179544 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 208 0.14353439649996894 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 197 0.1359436351466052 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 194 0.13387342750477874 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.126282666151415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 182 0.12559259693747282 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 173 0.11938197401199341 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 0.1180018355841091 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 159 0.10972100501680318 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 149 0.1028203128773816 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0010351038209132377 0.0 18-19 0.0 0.0 0.0 0.0020702076418264753 0.0 20-21 0.0 0.0 0.0 0.003105311462739713 0.0 22-23 0.0 0.0 0.0 0.007245726746392663 0.0 24-25 0.0 0.0 0.0 0.017941799562496117 0.0 26-27 0.0 0.0 0.0 0.034848495304079 0.0 28-29 0.0 0.0 0.0 0.08694872095671197 0.0 30-31 0.0 0.0 0.0 0.1842484801225563 0.0 32-33 0.0 0.0 0.0 0.33744384561771545 0.0 34-35 0.0 0.0 0.0 0.6010502853436199 0.0 36-37 0.0 0.0 0.0 0.9647167610911375 0.0 38-39 0.0 0.0 0.0 1.4249929267905572 0.0 40-41 0.0 0.0 0.0 1.8600815661810879 0.0 42-43 0.0 0.0 0.0 2.181308785271163 0.0 44-45 0.0 0.0 0.0 2.521512907744647 0.0 46-47 0.0 0.0 0.0 2.9017410446267764 0.0 48-49 0.0 0.0 0.0 3.246775651597855 0.0 50-51 0.0 0.0 0.0 3.606646746668691 0.0 52-53 0.0 0.0 0.0 3.95168135363977 0.0 54-55 0.0 0.0 0.0 4.325008798382478 0.0 56-57 0.0 0.0 0.0 4.794945933077088 0.0 58-59 0.0 0.0 0.0 5.180004554456811 0.0 60-61 0.0 0.0 0.0 5.591285805966338 0.0 62-63 0.0 0.0 0.0 5.999116711406154 0.0 64-65 0.0 0.0 0.0 6.421094035731784 0.0 66-67 0.0 0.0 0.0 6.933815461690807 0.0 68-69 0.0 0.0 0.0 7.518304085899816 0.0 70-71 0.0 0.0 0.0 8.10520795235762 0.0 72-73 0.0 0.0 0.0 8.699012510954848 0.0 74-75 0.0 0.0 0.0 9.350092814309274 0.0 76-77 0.0 0.0 0.0 9.979090902817553 0.0 78-79 0.0 0.0 0.0 10.761974426034932 0.0 80-81 0.0 0.0 0.0 11.515875042266739 0.0 82-83 0.0 0.0 0.0 12.237342405443266 0.0 84-85 0.0 0.0 0.0 13.01539544416305 0.0 86-87 0.0 0.0 0.0 13.761015229827551 0.0 88 0.0 0.0 0.0 14.427277055888705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACAGT 60 1.5957739E-6 46.998272 6 GCCACAC 60 8.787849E-5 39.165226 3 GCTAGCC 25 0.0017002348 37.598618 44-45 CAGTGCA 105 5.061156E-8 35.80821 9 GTTAAAG 55 0.0026868433 34.23964 1 AAGGTGC 55 0.0027097035 34.18056 6 CATTGTT 35 2.1814581E-4 33.570198 14-15 TGCCACA 75 3.2293503E-4 31.386335 2 ACCACTT 30 0.0041566426 31.332182 90-91 GCATTGT 40 4.7731923E-4 29.37392 12-13 CATACTG 80 4.7615517E-4 29.37392 9 ATGAACC 80 4.7615517E-4 29.37392 94 TATACTC 65 0.0061377767 28.922014 3 AGGTGCA 65 0.0061377767 28.922014 7 CACAGTG 115 4.164409E-6 28.607647 7 CATGGGG 250 0.0 28.198963 4 ATGCCAC 85 6.7203917E-4 27.693823 1 CCTGAAC 85 6.788634E-4 27.646044 3 TGTCTTA 60 5.8251007E-6 27.415659 34-35 GTGCAAT 70 0.008812598 26.856157 9 >>END_MODULE