FastQCFastQC Report
Wed 25 May 2016
SRR1033070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884827
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.035.1408271
GTATCAA34350.031.8910431
TCAACGC34800.029.7187274
ATCAACG34950.029.591183
CAACGCA37050.028.0408275
AACGCAG39700.026.1631816
GTACATG76950.024.9282071
TATCAAC45100.023.6611632
TACATGG77850.023.2481752
ACATGGG78500.021.9777493
ACGCAGA47000.021.7995457
CGCAGAG47900.020.9156888
CCTATAC10600.019.513433
GAGTACA57900.018.1078051
CTGTCGC8100.018.0047749
CTATACT17300.017.9343074
TACACCG3701.6552804E-1017.7874245
GTATTAG14700.017.5907731
CATGGGG57650.017.1240624
TATACTG17750.016.949955
Produced by FastQC (version 0.11.5)