FastQCFastQC Report
Wed 25 May 2016
SRR1033070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884827
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.1550526253394051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.14517335008303792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.051.8828281
GTATCAA70650.041.1111561
TCAACGC82750.034.5894
ATCAACG82900.034.5270163
CAACGCA85200.033.593785
AACGCAG88200.032.557716
TATCAAC91150.031.6933732
ACGCAGA102050.028.0464537
GTACATG76200.027.8450931
CGCAGAG104000.027.2951058
TACATGG77800.025.9861742
ACATGGG79850.024.3681013
GCAGAGT119250.023.5680689
GACGCGT500.001759925423.499110-11
GAGTACT79300.022.16522212-13
GTGGTAT20150.021.060011
CAGAGTA114900.020.90172210-11
AGAGTAC105950.020.11673710-11
CATGGGG57450.019.5522424
GTACTTT89900.019.36860814-15
Produced by FastQC (version 0.11.5)