FastQCFastQC Report
Wed 25 May 2016
SRR1033069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4196395
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC47250.035.618044
GTATCAA51900.034.5142331
CAACGCA49000.034.2500235
ATCAACG49100.034.180273
GGTATCA31050.033.1606561
AACGCAG55550.030.2900286
TATCAAC63650.027.1792932
GTACATG103450.026.0414241
TACATGG103250.024.859442
ACGCAGA67400.024.476427
CGCAGAG67100.024.1182678
ACATGGG109200.023.2466223
CTGTCGC11850.019.8531429
TCTGTCG10200.018.4488778
CATGGGG75400.018.1430554
GTGTACG3651.364242E-1018.0333211
GCAGAGT86550.017.9944489
GAGTACA78500.017.8479021
TAGACCG2403.4159057E-617.6286895
GTCTAGG14850.017.0965251
Produced by FastQC (version 0.11.5)