FastQCFastQC Report
Wed 25 May 2016
SRR1033069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4196395
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58630.13971516027447367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49950.11903073947995839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.056.636771
GTATCAA104550.042.5961571
TCAACGC120750.036.3919754
ATCAACG123050.035.636643
CAACGCA124150.035.320055
TATCAAC131800.033.4900362
AACGCAG131350.033.3839656
ACGCAGA149200.029.2009687
CGCAGAG149950.028.835868
GTACATG101200.025.6586461
GCAGAGT170950.024.82599
TACATGG99950.024.6496262
GTGGTAT24150.023.4167581
ACATGGG106750.022.058023
GAGTACT119100.021.7822812-13
CAGAGTA167800.021.00659610-11
AGAGTAC156150.020.49705510-11
TGGTATC24250.020.3971272
GTACTTT137400.018.74421314-15
AGTACTT130250.018.36606212-13
Produced by FastQC (version 0.11.5)