##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033069_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4196395
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68864990068857	34.0	31.0	34.0	31.0	34.0
2	32.69313088972797	34.0	31.0	34.0	31.0	34.0
3	32.805632215270485	34.0	31.0	34.0	31.0	34.0
4	36.297433392233096	37.0	37.0	37.0	35.0	37.0
5	36.27669678378704	37.0	37.0	37.0	35.0	37.0
6	36.341067034919256	37.0	37.0	37.0	35.0	37.0
7	36.295124982276455	37.0	37.0	37.0	35.0	37.0
8	36.29902571135462	37.0	37.0	37.0	35.0	37.0
9	38.09808919322418	39.0	39.0	39.0	35.0	39.0
10-11	38.06915566813896	39.0	39.0	39.0	35.0	39.0
12-13	38.04143580382686	39.0	39.0	39.0	35.0	39.0
14-15	39.53626577097723	41.0	39.0	41.0	37.0	41.0
16-17	39.5361573445779	41.0	39.0	41.0	37.0	41.0
18-19	39.556078133731454	41.0	39.5	41.0	37.0	41.0
20-21	39.551550080485754	41.0	40.0	41.0	37.0	41.0
22-23	39.479600466590966	41.0	39.0	41.0	36.5	41.0
24-25	39.42798985796142	41.0	39.0	41.0	36.0	41.0
26-27	39.317119932704145	41.0	39.0	41.0	36.0	41.0
28-29	39.26780903608931	41.0	39.0	41.0	36.0	41.0
30-31	39.264783105498886	41.0	39.0	41.0	36.0	41.0
32-33	39.22560280907779	41.0	39.0	41.0	35.5	41.0
34-35	39.14391150022817	41.0	39.0	41.0	35.0	41.0
36-37	39.05304600734678	41.0	39.0	41.0	35.0	41.0
38-39	38.96074928599429	41.0	39.0	41.0	35.0	41.0
40-41	38.86412861515658	41.0	39.0	41.0	35.0	41.0
42-43	38.75075225282653	41.0	39.0	41.0	35.0	41.0
44-45	38.592948590397235	41.0	39.0	41.0	35.0	41.0
46-47	38.403934329346974	41.0	38.0	41.0	34.5	41.0
48-49	38.20840292679789	40.5	38.0	41.0	34.0	41.0
50-51	38.084111719702264	40.0	38.0	41.0	34.0	41.0
52-53	37.957565720100234	40.0	38.0	41.0	34.0	41.0
54-55	37.82295243893866	40.0	38.0	41.0	33.0	41.0
56-57	37.68574347743718	40.0	37.0	41.0	33.0	41.0
58-59	37.519326826955044	40.0	37.0	41.0	33.0	41.0
60-61	37.33496894358133	40.0	36.5	41.0	33.0	41.0
62-63	37.114835114425595	39.5	36.0	41.0	32.5	41.0
64-65	36.85568160289963	39.0	35.5	41.0	32.0	41.0
66-67	36.58503334409654	39.0	35.0	41.0	32.0	41.0
68-69	36.28460261724647	38.5	35.0	40.5	31.5	41.0
70-71	35.95643451105056	37.5	35.0	40.0	31.0	41.0
72-73	35.60695275349437	37.0	35.0	39.5	31.0	41.0
74-75	35.25520428844282	36.5	35.0	39.0	31.0	41.0
76-77	34.88624771976899	36.0	35.0	39.0	31.0	40.5
78-79	34.514501494735356	35.5	35.0	37.5	30.0	39.5
80-81	34.16813026419105	35.0	34.0	37.0	30.0	39.0
82-83	33.85318719996569	35.0	34.0	37.0	30.0	39.0
84-85	33.612229306345085	35.0	34.0	36.0	30.0	38.0
86-87	33.38763164573402	35.0	34.0	36.0	30.0	37.0
88-89	33.16707090252467	35.0	34.0	36.0	30.0	37.0
90-91	32.979154369405165	35.0	34.0	35.0	30.0	36.5
92-93	32.801721835051275	35.0	34.0	35.0	29.0	36.0
94-95	32.640501906993975	35.0	34.0	35.0	29.0	36.0
96-97	32.49518801256793	35.0	34.0	35.0	29.0	36.0
98-99	32.36799908016286	35.0	34.0	35.0	29.0	35.5
100	32.26207947535921	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12543339160706068
1101	2	0.12819533788233883
1101	3	0.172200832681618
1101	4	0.11868775965324119
1101	5	0.07722790185568584
1101	6	0.0991691132508663
1101	7	0.17603078768705416
1101	8	0.17126749279242404
1101	9	0.2457422103792979
1101	10-11	0.2607030343684045
1101	12-13	0.2627022160123147
1101	14-15	0.33044433653141425
1101	16-17	0.3338028100952002
1101	18-19	0.33479142741864365
1101	20-21	0.2810102100292653
1101	22-23	0.31998041938677346
1101	24-25	0.3696198084641864
1101	26-27	0.3762832012088353
1101	28-29	0.35235929939539545
1101	30-31	0.4031948472460911
1101	32-33	0.4008484755807302
1101	34-35	0.4051777611727516
1101	36-37	0.3851373980603867
1101	38-39	0.34072895115122037
1101	40-41	0.3696850126562339
1101	42-43	0.36716197444835785
1101	44-45	0.3604078434600666
1101	46-47	0.316905168439483
1101	48-49	0.32678116859921147
1101	50-51	0.353070887624348
1101	52-53	0.29339703179877574
1101	54-55	0.3218936072841032
1101	56-57	0.29925810285345733
1101	58-59	0.30668387246237927
1101	60-61	0.3348305508537095
1101	62-63	0.37164373443951604
1101	64-65	0.32969657179707923
1101	66-67	0.34893082466282976
1101	68-69	0.30300876858253645
1101	70-71	0.2791858675264791
1101	72-73	0.25471626999733843
1101	74-75	0.25398417272079143
1101	76-77	0.2886772769369159
1101	78-79	0.22037324798036195
1101	80-81	0.2232446802543393
1101	82-83	0.21035033197698993
1101	84-85	0.19650400135397206
1101	86-87	0.23737564266463806
1101	88-89	0.2958575754082631
1101	90-91	0.2788266001553339
1101	92-93	0.25724480008862827
1101	94-95	0.2941256805134955
1101	96-97	0.2857038355252328
1101	98-99	0.2601497005904534
1101	100	0.29512716872366695
1102	1	0.1501737608233924
1102	2	0.12181300528795447
1102	3	0.16789273203382749
1102	4	0.12095081744536174
1102	5	0.11853217692607387
1102	6	0.12290592919453758
1102	7	0.15870102474050896
1102	8	0.13946497990644247
1102	9	0.2193190472858717
1102	10-11	0.19694065372516434
1102	12-13	0.26282458200586234
1102	14-15	0.2982657882884041
1102	16-17	0.32120443715407276
1102	18-19	0.2755406153534139
1102	20-21	0.2549848077237229
1102	22-23	0.29175813390567384
1102	24-25	0.31720147801933507
1102	26-27	0.2609271057089799
1102	28-29	0.24929647001979305
1102	30-31	0.25884114886466847
1102	32-33	0.22805713897898272
1102	34-35	0.27787581628555813
1102	36-37	0.2319437311993795
1102	38-39	0.2327238477191571
1102	40-41	0.273632889496902
1102	42-43	0.2862421605891896
1102	44-45	0.255795520225206
1102	46-47	0.27935782411432086
1102	48-49	0.2969392688856942
1102	50-51	0.28321326383313306
1102	52-53	0.3108626618819059
1102	54-55	0.2221485887304837
1102	56-57	0.2499470794934382
1102	58-59	0.2981280935358015
1102	60-61	0.3052917613091495
1102	62-63	0.26935161115848416
1102	64-65	0.292322507987258
1102	66-67	0.3102321751194168
1102	68-69	0.30251797350973675
1102	70-71	0.2790677325249362
1102	72-73	0.30434571116030895
1102	74-75	0.35038846654065026
1102	76-77	0.2564448192002473
1102	78-79	0.3088710537593826
1102	80-81	0.2956460334872162
1102	82-83	0.3195520930584763
1102	84-85	0.30447944030274954
1102	86-87	0.32013213490795067
1102	88-89	0.3555484106422142
1102	90-91	0.32792041674591843
1102	92-93	0.3257099521172009
1102	94-95	0.31736832609859533
1102	96-97	0.33056851413908106
1102	98-99	0.3065824609048491
1102	100	0.3003991749212602
1103	1	0.13948957431476572
1103	2	0.13343044252729896
1103	3	0.16355826074834567
1103	4	0.13878402947415225
1103	5	0.12401254291921049
1103	6	0.12969790056666142
1103	7	0.14647525965541774
1103	8	0.13413443946581793
1103	9	0.22437990173197164
1103	10-11	0.21248550608020622
1103	12-13	0.21578675953794857
1103	14-15	0.27782507010773827
1103	16-17	0.2858470508626425
1103	18-19	0.27161951818270325
1103	20-21	0.291140962774044
1103	22-23	0.29574150796266707
1103	24-25	0.252851042189441
1103	26-27	0.27639722580047277
1103	28-29	0.31493558611402506
1103	30-31	0.319984956845353
1103	32-33	0.3019597660888138
1103	34-35	0.36739044647999464
1103	36-37	0.35829577142627755
1103	38-39	0.40599114228123767
1103	40-41	0.40167940256891654
1103	42-43	0.4108130818683122
1103	44-45	0.44453293487879364
1103	46-47	0.46404002065078487
1103	48-49	0.46810070212775656
1103	50-51	0.5144715313154293
1103	52-53	0.47451241956163415
1103	54-55	0.4433726959868025
1103	56-57	0.474891914808083
1103	58-59	0.5167699664201919
1103	60-61	0.47531957470810227
1103	62-63	0.4513761190086285
1103	64-65	0.4108827169198932
1103	66-67	0.48195590546519185
1103	68-69	0.44969684861226256
1103	70-71	0.430035388978915
1103	72-73	0.45501696573811046
1103	74-75	0.4751747394874286
1103	76-77	0.44846214986487354
1103	78-79	0.4111354774472744
1103	80-81	0.3809442477110423
1103	82-83	0.3825519327631852
1103	84-85	0.41668794837239176
1103	86-87	0.4071811419436102
1103	88-89	0.448568417227321
1103	90-91	0.39649900187282583
1103	92-93	0.5026088014569652
1103	94-95	0.4455027488622605
1103	96-97	0.44327415422272054
1103	98-99	0.45374111858342303
1103	100	0.4818848523204906
1104	1	0.08215253343144013
1104	2	0.08671182688992474
1104	3	0.1111832789495466
1104	4	0.04362506775299124
1104	5	0.09861156706018193
1104	6	0.09217037554516594
1104	7	0.08577597643926538
1104	8	0.12232091659274857
1104	9	0.13649609681586128
1104	10-11	0.1579413619898915
1104	12-13	0.15342150851250835
1104	14-15	0.23970905394507724
1104	16-17	0.185834550416196
1104	18-19	0.21702905547386564
1104	20-21	0.2370636107669597
1104	22-23	0.17331135420717914
1104	24-25	0.1840410211584711
1104	26-27	0.1724418905137739
1104	28-29	0.15079811348450534
1104	30-31	0.18182209835040197
1104	32-33	0.1359842298746088
1104	34-35	0.12951866980939997
1104	36-37	0.137795365505184
1104	38-39	0.0837513541844146
1104	40-41	0.11498677171946525
1104	42-43	0.12468769311816175
1104	44-45	0.10479247883228737
1104	46-47	0.07456311282165728
1104	48-49	0.019756908255999406
1104	50-51	0.09840262809910172
1104	52-53	0.17355133613008888
1104	54-55	0.14503941073211735
1104	56-57	0.1335370037810435
1104	58-59	0.11300318525767494
1104	60-61	0.1507731279943485
1104	62-63	0.16875875389668238
1104	64-65	0.11327383081956555
1104	66-67	0.09615216281778771
1104	68-69	0.1454633698067056
1104	70-71	0.10460948949624793
1104	72-73	0.11654501309968879
1104	74-75	0.10436616665379006
1104	76-77	0.07231680504487059
1104	78-79	0.06024099151782991
1104	80-81	0.09402633409050054
1104	82-83	0.1674123381485444
1104	84-85	0.15390272183957876
1104	86-87	0.16715917687343307
1104	88-89	0.16984185899859483
1104	90-91	0.1874878455677944
1104	92-93	0.13306486856924238
1104	94-95	0.10528506938469917
1104	96-97	0.16793070102246332
1104	98-99	0.21568342352838243
1104	100	0.19710794064507553
1105	1	0.08265222211146295
1105	2	0.018208384735103778
1105	3	0.07197709695095256
1105	4	0.06421448833469867
1105	5	0.0175419338727707
1105	6	0.07740688382033056
1105	7	0.09389608917096126
1105	8	0.10246134748859959
1105	9	0.0728043179568445
1105	10-11	0.117769232411014
1105	12-13	0.11798017347707912
1105	14-15	0.12404083481262518
1105	16-17	0.1483798883532259
1105	18-19	0.17076377855209302
1105	20-21	0.1601146031026488
1105	22-23	0.19042576703782288
1105	24-25	0.127204970440026
1105	26-27	0.18066433902566104
1105	28-29	0.18840038024881522
1105	30-31	0.19524930536207563
1105	32-33	0.16157792641906354
1105	34-35	0.17385280696824168
1105	36-37	0.160680329677497
1105	38-39	0.20394454260171813
1105	40-41	0.14441651488947826
1105	42-43	0.1825998695981781
1105	44-45	0.17269864866484852
1105	46-47	0.16377299352183883
1105	48-49	0.1884020300476692
1105	50-51	0.24023813781548142
1105	52-53	0.22519275067729438
1105	54-55	0.20843276631479313
1105	56-57	0.13693983672001764
1105	58-59	0.18543790908031355
1105	60-61	0.17751236447740837
1105	62-63	0.21298798692331644
1105	64-65	0.21991875631771762
1105	66-67	0.2337042107461471
1105	68-69	0.2631198642336443
1105	70-71	0.27051567674416077
1105	72-73	0.2381617689952975
1105	74-75	0.198947515248463
1105	76-77	0.19124169453774442
1105	78-79	0.21313601731218768
1105	80-81	0.19214769583788183
1105	82-83	0.21363958423213347
1105	84-85	0.2136173183224983
1105	86-87	0.1759368014565652
1105	88-89	0.2014326849770285
1105	90-91	0.21010355854767226
1105	92-93	0.20537526767274272
1105	94-95	0.2305596529978331
1105	96-97	0.21925870795080726
1105	98-99	0.28171037001851573
1105	100	0.2611993660658882
1106	1	-0.016110894532261
1106	2	0.045507911988302396
1106	3	0.03647081635440941
1106	4	0.020034374343417483
1106	5	0.005206832847626686
1106	6	0.030471814567874844
1106	7	0.04594838700436554
1106	8	0.02437850946617459
1106	9	0.03997430963851656
1106	10-11	0.07657125601648573
1106	12-13	0.034920525129322755
1106	14-15	0.03316981839478217
1106	16-17	0.09046076663080527
1106	18-19	0.07730142544330221
1106	20-21	0.0771502544278988
1106	22-23	0.07315285302287577
1106	24-25	0.06886417886189378
1106	26-27	0.09458177979583837
1106	28-29	0.08115782578042285
1106	30-31	0.07698573449817303
1106	32-33	0.013488066827079592
1106	34-35	-0.004919422714920074
1106	36-37	0.032065349052295744
1106	38-39	0.03972309521786599
1106	40-41	0.06427164414906628
1106	42-43	0.05032593322988532
1106	44-45	0.009535139305036466
1106	46-47	0.003645819753380408
1106	48-49	-0.04694692924443444
1106	50-51	-0.001955426578554409
1106	52-53	0.03418902141915936
1106	54-55	0.036060142833143516
1106	56-57	-0.01533766520903157
1106	58-59	-0.01582845872062677
1106	60-61	-0.020934433439130373
1106	62-63	-0.06261781359144436
1106	64-65	-0.07333128934862998
1106	66-67	-0.09081119468572041
1106	68-69	-0.0299167521994832
1106	70-71	-0.030825685528611757
1106	72-73	-0.03661457626762399
1106	74-75	0.00843984344744797
1106	76-77	0.02810588841850148
1106	78-79	0.022449121701598074
1106	80-81	4.403121505731633E-4
1106	82-83	-0.02294320041985287
1106	84-85	-0.06368775877382404
1106	86-87	-0.11232827660023759
1106	88-89	-0.08597065021991313
1106	90-91	-0.06380799636757928
1106	92-93	-0.07082103615012869
1106	94-95	-0.06934387299548916
1106	96-97	-0.06062631716429934
1106	98-99	-0.12231818294573316
1106	100	-0.08064905982389803
1107	1	-0.023988943027120513
1107	2	-0.027430695143692674
1107	3	-0.0274180846808747
1107	4	9.260494300420419E-4
1107	5	-0.028431951217910978
1107	6	-0.015269398653501298
1107	7	0.011159959623036286
1107	8	-0.04268318798304449
1107	9	-0.02014758517229609
1107	10-11	0.022398407884047344
1107	12-13	-0.001744029573181649
1107	14-15	0.051121966071846714
1107	16-17	0.0014010299608742116
1107	18-19	-0.0127553372373157
1107	20-21	-0.0190119418057364
1107	22-23	0.036689756681376196
1107	24-25	0.03625745267321889
1107	26-27	0.001584489010589607
1107	28-29	0.012928076950863954
1107	30-31	0.016402570028994035
1107	32-33	-0.019321700941809183
1107	34-35	0.018855893148611358
1107	36-37	0.04228842162239488
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2216	30-31	-0.3154426814907012
2216	32-33	-0.34316509216377256
2216	34-35	-0.34088278830613206
2216	36-37	-0.338153770161
2216	38-39	-0.3383463627755532
2216	40-41	-0.2836469295948021
2216	42-43	-0.29914029213945525
2216	44-45	-0.27717957961035466
2216	46-47	-0.1724349044667548
2216	48-49	-0.17497445177691873
2216	50-51	-0.1932227923424037
2216	52-53	-0.13571155838969418
2216	54-55	-0.17015165836811263
2216	56-57	-0.20764993867172166
2216	58-59	-0.20826011887979234
2216	60-61	-0.2589300862509347
2216	62-63	-0.30392568639758366
2216	64-65	-0.34868389100413566
2216	66-67	-0.3134271440064893
2216	68-69	-0.2934654105219323
2216	70-71	-0.3522078582369659
2216	72-73	-0.2864161910528793
2216	74-75	-0.35674059564433946
2216	76-77	-0.35312824437635015
2216	78-79	-0.4064355298869131
2216	80-81	-0.3708351605550604
2216	82-83	-0.25970493111318405
2216	84-85	-0.33420910841670803
2216	86-87	-0.32823407056746845
2216	88-89	-0.4842983941736776
2216	90-91	-0.4899123858160621
2216	92-93	-0.4328938961981592
2216	94-95	-0.3461519963487163
2216	96-97	-0.5160132885804884
2216	98-99	-0.5478869461477203
2216	100	-0.5788622351761816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24.0
3	15.0
4	9.0
5	32.0
6	88.0
7	223.0
8	497.0
9	1002.0
10	1815.0
11	2891.0
12	4790.0
13	7222.0
14	8694.0
15	11368.0
16	13360.0
17	18955.0
18	22215.0
19	11581.0
20	7815.0
21	7415.0
22	7672.0
23	8411.0
24	9580.0
25	11816.0
26	15325.0
27	19710.0
28	25658.0
29	33444.0
30	42870.0
31	55006.0
32	70510.0
33	91447.0
34	126308.0
35	187610.0
36	333954.0
37	764046.0
38	1506892.0
39	763773.0
40	2352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.105741224925936	20.383390435575155	13.858858325487786	23.65201001401113
2	15.240274009186669	20.760923234286835	40.41465901851356	23.584143738012934
3	17.713619272716702	25.86082319695997	30.80907014174956	25.616487388573766
4	11.89009082046307	17.272354866821992	40.01524199527155	30.822312317443384
5	12.057467530997497	38.13532927253849	36.605751566764454	13.201451629699548
6	29.834159876903747	39.348740381913885	17.85055301090866	12.966546730273704
7	26.044402397772377	33.2142231605938	23.25465071805681	17.486723723577022
8	24.52689051960425	36.40618379720074	20.93325065901945	18.133675024175563
9	24.723721194024872	16.286240928225297	21.93694826154354	37.05308961620629
10-11	23.271110117660008	26.854218425184335	29.788980739427085	20.08569071772857
12-13	24.374075843670578	24.328941865577477	29.795419639952865	21.501562650799077
14-15	21.37547469440356	26.247502611772422	27.35247775227434	25.024544941549674
16-17	20.425916135763853	29.72082310281255	27.859318111438412	21.993942649985186
18-19	20.264430452217418	28.28974514328132	30.624902073276296	20.820922331224967
20-21	21.57872669025755	27.58020009796505	30.177715655665832	20.66335755611156
22-23	21.084391325784466	27.424672772389417	30.288133303116894	21.202802598709226
24-25	20.954139061248164	27.650704018599075	30.18673662607731	21.208420294075452
26-27	20.735896455812515	27.554635029329962	30.299647887995214	21.409820626862313
28-29	20.795882931100078	27.58593202932954	30.387749356082445	21.230435683487936
30-31	21.124985731787145	28.059849523078285	29.848970222269568	20.966194522865006
32-33	20.71893851746559	27.68830150641205	30.41262798187492	21.180131994247443
34-35	20.932050009591567	27.527758945475817	30.360356925408595	21.17983411952402
36-37	21.3210029084488	27.889057155010434	29.6768059250857	21.11313401145507
38-39	21.13209075885373	28.00672958575158	29.83401228911959	21.0271673662751
40-41	20.94691854821423	27.78851253237753	29.960915219877656	21.303653699530585
42-43	21.031027822690668	27.640462825830266	29.792500467663313	21.536008883815754
44-45	21.15051133175023	27.887901401083546	29.61197647027985	21.349610796886374
46-47	21.27143655447116	28.13319527832818	29.26868180902894	21.326686358171717
48-49	21.30624023715594	28.0855829825362	29.004037989750724	21.604138790557133
50-51	21.35239890429762	28.023803764898204	28.86794498611308	21.7558523446911
52-53	21.360048327195127	28.0569989240765	28.884804695458836	21.698148053269534
54-55	21.470976874197973	28.494469657884924	28.66660550305679	21.367947964860313
56-57	21.292192465199296	28.07174968038042	28.757790913391133	21.878266941029146
58-59	21.11131101814772	27.77668689434622	29.18673051512072	21.925271572385345
60-61	21.44749243100328	28.127809703328687	28.760745830647494	21.663952035020536
62-63	21.258818581186947	28.123484562344586	28.766322045469984	21.85137481099849
64-65	21.26339393693873	27.850202376087097	28.959023161546995	21.927380525427182
66-67	21.113398656870796	27.899438434589502	28.817965041179995	22.169197867359706
68-69	21.645878957866167	28.07936106987196	28.61153276983312	21.663227202428754
70-71	21.577702001086884	27.813471779166616	28.696479799504075	21.91234642024242
72-73	21.34325822267065	27.97831276722399	28.822288988667864	21.856140021437493
74-75	21.457473814999602	28.082023338625977	28.595762406315334	21.864740440059087
76-77	21.43923534366998	28.108161886571686	28.49118112093833	21.961421648819996
78-79	21.586909716554327	28.116847913506714	28.575491582656063	21.720750787282896
80-81	21.489028082437425	27.90754921784055	28.647553435746637	21.955869263975387
82-83	21.283876567790074	27.986264392570526	28.797139447751846	21.932719591887555
84-85	21.266193959338906	27.915234385704874	28.644538943545587	22.174032711410625
86-87	21.409415453040694	28.007772417070147	28.503163918979652	22.079648210909504
88-89	21.157397119494796	27.90726381793539	28.603077106388046	22.332261956181767
90-91	21.077432772901478	27.802412472364164	28.720955933386833	22.399198821347525
92-93	21.145224015343718	28.000187304496464	28.49332715816304	22.36126152199678
94-95	20.946456682405636	27.691892865704666	28.74853028016065	22.613120171729047
96-97	20.93560179106327	27.65841592416339	28.734864967269484	22.671117317503857
98-99	20.953126264107212	27.7346805546338	28.578870341070502	22.733322840188492
100	21.01896985388649	27.785396751259118	28.439505813918853	22.75612758093554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	23.5
2	70.0
3	143.5
4	107.5
5	79.0
6	212.5
7	481.5
8	846.5
9	1044.0
10	920.0
11	842.0
12	1037.5
13	1256.5
14	1448.5
15	1703.0
16	2001.0
17	2342.0
18	2815.5
19	3407.0
20	4128.5
21	5281.0
22	6974.5
23	9715.5
24	13438.5
25	18352.5
26	25518.0
27	34245.5
28	45914.0
29	58902.5
30	71108.0
31	83920.0
32	97594.0
33	111749.0
34	125599.5
35	137988.0
36	147482.0
37	155698.0
38	162154.0
39	165421.0
40	166680.5
41	166314.5
42	164598.0
43	160345.0
44	153795.5
45	149012.5
46	145086.5
47	139544.5
48	134401.0
49	130163.5
50	125993.0
51	120328.0
52	111444.5
53	102694.5
54	96689.5
55	91190.0
56	83237.5
57	75049.5
58	67963.5
59	59950.0
60	51994.0
61	43683.0
62	35625.0
63	28899.0
64	22744.5
65	17605.5
66	13566.0
67	10156.5
68	7767.5
69	5912.5
70	3958.5
71	2503.5
72	1808.5
73	1318.5
74	906.5
75	595.0
76	350.5
77	231.0
78	146.0
79	80.5
80	45.0
81	16.0
82	6.5
83	4.5
84	3.5
85	1.5
86	2.0
87	1.0
88	0.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0446097185798763
2	0.24096873626052837
3	0.002883427322737731
4	0.002240017920143361
5	1.9063982299092437E-4
6	9.531991149546218E-5
7	0.0
8	2.1446980086478989E-4
9	0.0
10-11	5.838344579097059E-4
12-13	0.0
14-15	2.6212975661252095E-4
16-17	1.3106487830626048E-4
18-19	4.1702461279264697E-4
20-21	1.1914988936932773E-5
22-23	9.531991149546218E-5
24-25	0.0013583087388103362
26-27	7.148993362159664E-5
28-29	2.7404474554945375E-4
30-31	9.531991149546218E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.0723490043239494E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.1914988936932773E-5
68-69	1.1914988936932773E-4
70-71	1.1914988936932773E-5
72-73	2.1446980086478989E-4
74-75	9.531991149546218E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	5.9574944684663865E-5
84-85	0.0
86-87	4.051096238557143E-4
88-89	0.0019659731745939072
90-91	0.0018468232852245797
92-93	4.646845685403781E-4
94-95	4.051096238557143E-4
96-97	1.1914988936932773E-4
98-99	1.0723490043239494E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4196395.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.027582641129605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03485763448674	39.23883773875953
2	16.20059519498252	18.153603722447407
3	6.104706494147793	10.260958428021185
4	2.88031900648851	6.455092446754053
5	1.522833544886057	4.266034114239395
6	0.8950934384591935	3.0089952956883197
7	0.5970491204534869	2.341585325591504
8	0.39963225904150457	1.7912343535607371
9	0.26922393664288735	1.357556972330665
>10	1.0394431376563447	9.458900512579168
>50	0.03834789664038265	1.4671892985923443
>100	0.01704810203825231	1.6086993038488224
>500	6.801873277014515E-4	0.23963572160448027
>1k	1.2753506138963244E-4	0.2120302452268697
>5k	4.251168712987748E-5	0.1396465207554636
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5863	0.13971516027447367	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4995	0.11903073947995839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.531991149546217E-5	0.0
4	0.0	0.0	0.0	1.1914988936932772E-4	0.0
5	0.0	0.0	0.0	1.1914988936932772E-4	0.0
6	0.0	0.0	0.0	1.1914988936932772E-4	0.0
7	0.0	0.0	0.0	1.1914988936932772E-4	0.0
8	0.0	0.0	0.0	1.1914988936932772E-4	0.0
9	0.0	0.0	0.0	1.6680984511705882E-4	0.0
10-11	2.3829977873865543E-5	0.0	0.0	2.7404474554945375E-4	0.0
12-13	2.3829977873865543E-5	0.0	0.0	4.051096238557142E-4	0.0
14-15	2.3829977873865543E-5	0.0	0.0	4.408545906665125E-4	0.0
16-17	4.7659955747731085E-5	0.0	0.0	4.646845685403781E-4	0.0
18-19	4.7659955747731085E-5	0.0	0.0	5.004295353511764E-4	0.0
20-21	4.7659955747731085E-5	0.0	0.0	5.957494468466387E-4	0.0
22-23	4.7659955747731085E-5	0.0	0.0	6.314944136574369E-4	0.0
24-25	4.7659955747731085E-5	0.0	0.0	7.506443030267647E-4	0.0
26-27	4.7659955747731085E-5	0.0	0.0	9.055391592068906E-4	0.0
28-29	4.7659955747731085E-5	0.0	0.0	0.0014417136613688653	0.0
30-31	5.9574944684663865E-5	0.0	0.0	0.004146416150052605	0.0
32-33	8.340492255852941E-5	0.0	0.0	0.010461360286626975	0.0
34-35	9.531991149546217E-5	0.0	0.0	0.020469950993650503	0.0
36-37	9.531991149546217E-5	0.0	0.0	0.03738923528409503	0.0
38-39	1.1914988936932772E-4	0.0	0.0	0.06600903871060756	0.0
40-41	1.1914988936932772E-4	0.0	0.0	0.10058633660558647	0.0
42-43	1.1914988936932772E-4	0.0	0.0	0.13578321392528586	0.0
44-45	1.6680984511705882E-4	0.0	0.0	0.1793444134787121	0.0
46-47	1.6680984511705882E-4	0.0	0.0	0.23361718808644089	0.0
48-49	1.6680984511705882E-4	0.0	0.0	0.29279893813618596	0.0
50-51	1.9063982299092434E-4	0.0	0.0	0.3606071401762703	0.0
52-53	1.9063982299092434E-4	0.0	0.0	0.4287966218623366	0.0
54-55	1.9063982299092434E-4	0.0	0.0	0.5023955085257703	0.0
56-57	1.9063982299092434E-4	0.0	0.0	0.5922226101213065	0.0
58-59	1.9063982299092434E-4	0.0	0.0	0.6925468169702804	0.0
60-61	1.9063982299092434E-4	0.0	0.0	0.8000795921260988	0.0
62-63	1.9063982299092434E-4	0.0	0.0	0.9130456022371584	1.1914988936932771E-5
64-65	1.9063982299092434E-4	0.0	0.0	1.0342329547147016	2.3829977873865543E-5
66-67	1.9063982299092434E-4	0.0	0.0	1.1749728040377514	2.3829977873865543E-5
68-69	1.9063982299092434E-4	0.0	0.0	1.3325842776954981	2.3829977873865543E-5
70-71	2.62129756612521E-4	0.0	0.0	1.5069839707653832	2.3829977873865543E-5
72-73	2.62129756612521E-4	0.0	0.0	1.6922263037678769	2.3829977873865543E-5
74-75	2.62129756612521E-4	0.0	0.0	1.883283151371594	2.3829977873865543E-5
76-77	3.097897123602521E-4	0.0	0.0	2.089126500246045	2.3829977873865543E-5
78-79	3.097897123602521E-4	0.0	0.0	2.323994762170863	2.3829977873865543E-5
80-81	3.3361969023411764E-4	0.0	0.0	2.5829908766929712	2.3829977873865543E-5
82-83	3.3361969023411764E-4	0.0	0.0	2.8569045573641185	2.3829977873865543E-5
84-85	3.3361969023411764E-4	0.0	0.0	3.132772296220923	2.3829977873865543E-5
86-87	3.3361969023411764E-4	0.0	0.0	3.424844420031956	2.3829977873865543E-5
88	3.3361969023411764E-4	0.0	0.0	3.664073567907692	2.3829977873865543E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5525	0.0	56.63677	1
GTATCAA	10455	0.0	42.596157	1
TCAACGC	12075	0.0	36.391975	4
ATCAACG	12305	0.0	35.63664	3
CAACGCA	12415	0.0	35.32005	5
TATCAAC	13180	0.0	33.490036	2
AACGCAG	13135	0.0	33.383965	6
ACGCAGA	14920	0.0	29.200968	7
CGCAGAG	14995	0.0	28.83586	8
GTACATG	10120	0.0	25.658646	1
GCAGAGT	17095	0.0	24.8259	9
TACATGG	9995	0.0	24.649626	2
GTGGTAT	2415	0.0	23.416758	1
ACATGGG	10675	0.0	22.05802	3
GAGTACT	11910	0.0	21.78228	12-13
CAGAGTA	16780	0.0	21.006596	10-11
AGAGTAC	15615	0.0	20.497055	10-11
TGGTATC	2425	0.0	20.397127	2
GTACTTT	13740	0.0	18.744213	14-15
AGTACTT	13025	0.0	18.366062	12-13
>>END_MODULE