FastQCFastQC Report
Wed 25 May 2016
SRR1033068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5307845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73200.041.8058431
TCAACGC77400.038.0782784
GGTATCA42400.036.918621
ATCAACG80200.036.807473
CAACGCA82950.035.644555
TATCAAC89600.033.207972
AACGCAG90500.032.9756976
ACGCAGA106250.027.5127747
CGCAGAG108950.026.6007258
GTACATG171300.026.5360451
TACATGG168300.025.8906082
ACATGGG179600.023.7122383
CATGGGG129800.020.931674
GCAGAGT128900.020.8086519
GTATTAA27850.018.7354831
GAGTACA125500.017.229841
AGAGTAC110550.016.73635310-11
AGTACAT124450.016.2412782
GTATATA26250.015.7587021
CAGAGTA124050.015.44630810-11
Produced by FastQC (version 0.11.5)