FastQCFastQC Report
Wed 25 May 2016
SRR1033068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5307845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111300.047.7252161
GGTATCA58000.047.619931
TCAACGC135350.038.788354
ATCAACG138900.037.8995863
CAACGCA140750.037.2313085
TATCAAC143250.036.800142
AACGCAG150900.034.851276
ACGCAGA174050.030.0267667
CGCAGAG177250.029.1667658
GTACATG165750.025.1656931
GCAGAGT201450.025.0330819
TACATGG165150.024.2753242
ACATGGG180850.021.5713943
CATGGGG123150.020.07514
AGAGTAC184500.019.91994510-11
CAGAGTA195900.018.89269310-11
GAGTACT115600.018.27467312-13
ATTATAC21650.017.802243
AGTACTT121850.017.26017812-13
GTACTTT121850.017.02883514-15
Produced by FastQC (version 0.11.5)