##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033068_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5307845 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71453367609642 34.0 31.0 34.0 31.0 34.0 2 32.71891699927183 34.0 31.0 34.0 31.0 34.0 3 32.831186479635335 34.0 31.0 34.0 31.0 34.0 4 36.314621847472935 37.0 37.0 37.0 35.0 37.0 5 36.2898545454888 37.0 37.0 37.0 35.0 37.0 6 36.348059146414414 37.0 37.0 37.0 35.0 37.0 7 36.302176495357344 37.0 37.0 37.0 35.0 37.0 8 36.30467864076664 37.0 37.0 37.0 35.0 37.0 9 38.11142450467186 39.0 39.0 39.0 37.0 39.0 10-11 38.07906881229576 39.0 39.0 39.0 35.0 39.0 12-13 38.05241166612815 39.0 39.0 39.0 35.0 39.0 14-15 39.556612994539215 41.0 40.0 41.0 37.0 41.0 16-17 39.549342341383365 41.0 40.0 41.0 37.0 41.0 18-19 39.55685282821936 41.0 40.0 41.0 37.0 41.0 20-21 39.54562746274618 41.0 40.0 41.0 37.0 41.0 22-23 39.47159242592804 41.0 39.0 41.0 36.0 41.0 24-25 39.415965142162214 41.0 39.0 41.0 36.0 41.0 26-27 39.309569043557225 41.0 39.0 41.0 36.0 41.0 28-29 39.256550634014374 41.0 39.0 41.0 36.0 41.0 30-31 39.26620596494434 41.0 39.0 41.0 36.0 41.0 32-33 39.24546072841238 41.0 39.0 41.0 35.5 41.0 34-35 39.170075426091 41.0 39.0 41.0 35.0 41.0 36-37 39.08742389802265 41.0 39.0 41.0 35.0 41.0 38-39 39.0002856149718 41.0 39.0 41.0 35.0 41.0 40-41 38.907279978974515 41.0 39.0 41.0 35.0 41.0 42-43 38.800264796730126 41.0 39.0 41.0 35.0 41.0 44-45 38.65852742497191 41.0 38.5 41.0 35.0 41.0 46-47 38.503235588077644 41.0 38.0 41.0 35.0 41.0 48-49 38.339281478641524 40.0 38.0 41.0 34.0 41.0 50-51 38.21121349624942 40.0 38.0 41.0 34.0 41.0 52-53 38.070943386628656 40.0 38.0 41.0 34.0 41.0 54-55 37.90909964401749 40.0 37.0 41.0 34.0 41.0 56-57 37.741597390277974 40.0 37.0 41.0 33.0 41.0 58-59 37.54417272923381 40.0 36.5 41.0 33.0 41.0 60-61 37.32982519271003 39.5 36.0 41.0 33.0 41.0 62-63 37.07298640031877 39.0 35.5 41.0 33.0 41.0 64-65 36.781999756963515 39.0 35.0 41.0 32.0 41.0 66-67 36.47920380116601 38.0 35.0 40.5 32.0 41.0 68-69 36.14914979619789 37.0 35.0 40.0 32.0 41.0 70-71 35.801452001706906 37.0 35.0 39.5 31.5 41.0 72-73 35.439109186496594 36.0 35.0 39.0 31.0 41.0 74-75 35.08230863938189 36.0 35.0 39.0 31.0 40.5 76-77 34.72555377935866 35.0 35.0 37.5 31.0 39.5 78-79 34.369950328240556 35.0 34.5 37.0 31.0 39.0 80-81 34.05327048924752 35.0 34.0 37.0 30.0 39.0 82-83 33.777684823125014 35.0 34.0 36.0 30.0 37.5 84-85 33.58842458662602 35.0 34.0 36.0 30.0 37.0 86-87 33.41409272501363 35.0 34.0 35.5 30.0 37.0 88-89 33.23404809296428 35.0 34.0 35.0 30.0 36.0 90-91 33.081677215517786 35.0 34.0 35.0 30.0 36.0 92-93 32.935993044258076 35.0 34.0 35.0 30.0 36.0 94-95 32.79832870025406 35.0 34.0 35.0 30.0 36.0 96-97 32.672304296753204 35.0 34.0 35.0 30.0 35.0 98-99 32.5628787200832 35.0 34.0 35.0 29.0 35.0 100 32.4669855657051 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14271675117336002 1101 2 0.11090861809947938 1101 3 0.13019179231714872 1101 4 0.11777367238912007 1101 5 0.08016397877347003 1101 6 0.11121552331584184 1101 7 0.14746120092343773 1101 8 0.16480464997378164 1101 9 0.21169758237174818 1101 10-11 0.2632754751234785 1101 12-13 0.25056545739027314 1101 14-15 0.2945767870175686 1101 16-17 0.3107066828158125 1101 18-19 0.3188388522786667 1101 20-21 0.28860599849479485 1101 22-23 0.3062786805421851 1101 24-25 0.2921147150407819 1101 26-27 0.3041907896560119 1101 28-29 0.3322075941523863 1101 30-31 0.29384652006481815 1101 32-33 0.29590571157938683 1101 34-35 0.3291733766706315 1101 36-37 0.2830468830081614 1101 38-39 0.24983998617720715 1101 40-41 0.2927963093805772 1101 42-43 0.2810086240881162 1101 44-45 0.2959458256849601 1101 46-47 0.29675323746792515 1101 48-49 0.30364475937545876 1101 50-51 0.3368779394252073 1101 52-53 0.2923595130004699 1101 54-55 0.3286388167380778 1101 56-57 0.3010476112100662 1101 58-59 0.2868789120136128 1101 60-61 0.2299212628979035 1101 62-63 0.25159430806922956 1101 64-65 0.27669258300492316 1101 66-67 0.302172441882405 1101 68-69 0.2795075750624534 1101 70-71 0.24016745556480856 1101 72-73 0.27139163330977567 1101 74-75 0.19253947354644652 1101 76-77 0.22078417732176803 1101 78-79 0.20215494360836317 1101 80-81 0.22977784654059263 1101 82-83 0.2420784828521363 1101 84-85 0.2485507498990671 1101 86-87 0.27667139688002607 1101 88-89 0.2946685601914041 1101 90-91 0.30710935393271654 1101 92-93 0.3013657362640956 1101 94-95 0.3492153812272889 1101 96-97 0.3284553803020174 1101 98-99 0.3558149826046204 1101 100 0.3616079615275396 1102 1 0.15426009257576823 1102 2 0.12964001458640695 1102 3 0.1458825843082252 1102 4 0.10590568835541347 1102 5 0.13162023861646333 1102 6 0.12711978999003293 1102 7 0.12792101733521122 1102 8 0.16473214913800405 1102 9 0.2073592129155486 1102 10-11 0.19418721341157408 1102 12-13 0.19509748463447352 1102 14-15 0.31387751645652173 1102 16-17 0.29309945206579613 1102 18-19 0.28166223621489195 1102 20-21 0.27191449357882647 1102 22-23 0.2976903523711769 1102 24-25 0.2724760511506332 1102 26-27 0.33657087125972396 1102 28-29 0.30959095843284246 1102 30-31 0.29962241998144634 1102 32-33 0.2771541759713969 1102 34-35 0.31282564655096934 1102 36-37 0.3110551642147428 1102 38-39 0.3161299170194667 1102 40-41 0.31247444594798424 1102 42-43 0.31227541508029333 1102 44-45 0.3078679839542886 1102 46-47 0.27914822202122025 1102 48-49 0.3415127514648191 1102 50-51 0.33688659924725073 1102 52-53 0.34158435823133004 1102 54-55 0.3107325200404887 1102 56-57 0.29380276380418024 1102 58-59 0.34420834372789244 1102 60-61 0.32641081270799077 1102 62-63 0.3620722899681823 1102 64-65 0.37597522751318735 1102 66-67 0.38035391952999476 1102 68-69 0.41617688666701724 1102 70-71 0.31477484339997375 1102 72-73 0.33035011852565077 1102 74-75 0.31707939532610396 1102 76-77 0.28153927352634867 1102 78-79 0.31634416273408306 1102 80-81 0.28172368841626394 1102 82-83 0.2492431765562486 1102 84-85 0.2557849235707863 1102 86-87 0.27128760565020826 1102 88-89 0.2848755097992921 1102 90-91 0.26505423718623433 1102 92-93 0.27618478626147436 1102 94-95 0.23568249605653335 1102 96-97 0.26043549896171214 1102 98-99 0.24674291274603632 1102 100 0.1980827292227758 1103 1 0.09663268935379676 1103 2 0.0929874163848794 1103 3 0.11142391232983329 1103 4 0.08967881035118097 1103 5 0.10240569372896147 1103 6 0.11248063055741397 1103 7 0.11874052511568323 1103 8 0.1432629284975846 1103 9 0.14685812789611674 1103 10-11 0.1743094526142812 1103 12-13 0.16160167805233527 1103 14-15 0.2430372603345532 1103 16-17 0.2476519863615394 1103 18-19 0.22714428586795776 1103 20-21 0.22676177720786939 1103 22-23 0.2095191781482768 1103 24-25 0.1837197075915924 1103 26-27 0.20429817656988547 1103 28-29 0.2303263322157747 1103 30-31 0.23456186055692285 1103 32-33 0.19259130096342858 1103 34-35 0.21906302546279477 1103 36-37 0.21382379638053095 1103 38-39 0.23247959795186546 1103 40-41 0.1665221519250082 1103 42-43 0.22439621211915295 1103 44-45 0.18303135534483772 1103 46-47 0.2166522484506146 1103 48-49 0.19791677918185968 1103 50-51 0.19070765919914834 1103 52-53 0.18324302104411316 1103 54-55 0.19639567359575238 1103 56-57 0.17791148495140163 1103 58-59 0.20372340599573135 1103 60-61 0.20397491442718518 1103 62-63 0.1507227626408394 1103 64-65 0.15807594031158345 1103 66-67 0.19755084130508038 1103 68-69 0.14914533618981096 1103 70-71 0.1627350443822948 1103 72-73 0.16002126076017475 1103 74-75 0.15000129694242048 1103 76-77 0.13070242003504973 1103 78-79 0.13806361259074862 1103 80-81 0.1301419163371591 1103 82-83 0.12064830384210978 1103 84-85 0.12996366124550462 1103 86-87 0.0879523340881434 1103 88-89 0.08058144261436695 1103 90-91 0.08126996234857131 1103 92-93 0.12777189142678935 1103 94-95 0.1576956002401957 1103 96-97 0.18734357531245394 1103 98-99 0.15698961665035682 1103 100 0.11584589091961561 1104 1 0.09240877227095012 1104 2 0.1394715226253993 1104 3 0.1369068338538142 1104 4 0.077848405945673 1104 5 0.10154421071285213 1104 6 0.08866858949795642 1104 7 0.11179030443953053 1104 8 0.13377796635464279 1104 9 0.16396816017286397 1104 10-11 0.18218465237231385 1104 12-13 0.16283199822726147 1104 14-15 0.2262269406110562 1104 16-17 0.22731308037747056 1104 18-19 0.1992595983287231 1104 20-21 0.2220362776423528 1104 22-23 0.21648899803289368 1104 24-25 0.23435738855035027 1104 26-27 0.30710547935769483 1104 28-29 0.2914653918024115 1104 30-31 0.26143424514370395 1104 32-33 0.26091377728774745 1104 34-35 0.25731234783741996 1104 36-37 0.2955042934977641 1104 38-39 0.2829313836785161 1104 40-41 0.3193340256241015 1104 42-43 0.26968325163627327 1104 44-45 0.23938020154115947 1104 46-47 0.23572393711976503 1104 48-49 0.12454124528801458 1104 50-51 0.17927838653862693 1104 52-53 0.20861434022598502 1104 54-55 0.1764581103070455 1104 56-57 0.20996789853059994 1104 58-59 0.16239916533992726 1104 60-61 0.1789490073227924 1104 62-63 0.15627488087706354 1104 64-65 0.13448534921268873 1104 66-67 0.17017681670369456 1104 68-69 0.21305105801379653 1104 70-71 0.1767812045071011 1104 72-73 0.2299021234620966 1104 74-75 0.21266058006435884 1104 76-77 0.2052146696649899 1104 78-79 0.19584305717488348 1104 80-81 0.21733977282044492 1104 82-83 0.2348922817312058 1104 84-85 0.207033041848959 1104 86-87 0.2212891148621523 1104 88-89 0.2562002075562546 1104 90-91 0.2540375602467648 1104 92-93 0.2375805703590217 1104 94-95 0.21213483725720295 1104 96-97 0.23854337192559427 1104 98-99 0.20073606633140884 1104 100 0.19805724467970975 1105 1 0.015993864939176206 1105 2 0.01734134072938076 1105 3 0.10840670990253898 1105 4 0.05490474896523523 1105 5 0.04859791032770744 1105 6 0.06469976155865709 1105 7 0.05308018860591801 1105 8 0.045683359358051234 1105 9 0.08233754824144057 1105 10-11 0.098167919797163 1105 12-13 0.13171392851758412 1105 14-15 0.13528614051197252 1105 16-17 0.15883503802971433 1105 18-19 0.13212717622561598 1105 20-21 0.1582769361915055 1105 22-23 0.1688565340960082 1105 24-25 0.17713523776338036 1105 26-27 0.1721439612092439 1105 28-29 0.1907326481649747 1105 30-31 0.15574768610863998 1105 32-33 0.2036096351738692 1105 34-35 0.1688853326090225 1105 36-37 0.200379763830405 1105 38-39 0.13005757234998327 1105 40-41 0.17332928148228177 1105 42-43 0.14398246370845413 1105 44-45 0.14943824830047703 1105 46-47 0.11770941703073845 1105 48-49 0.11750484297731134 1105 50-51 0.14868827680867014 1105 52-53 0.15653170645044412 1105 54-55 0.11358775923240216 1105 56-57 0.14474521286241782 1105 58-59 0.11414714328672915 1105 60-61 0.1237015515863007 1105 62-63 0.16969438639203815 1105 64-65 0.17846893775372052 1105 66-67 0.18362572842880098 1105 68-69 0.2018192826093994 1105 70-71 0.15552862540313583 1105 72-73 0.16257985421757581 1105 74-75 0.12370099070373897 1105 76-77 0.1571339299990413 1105 78-79 0.16686778366108257 1105 80-81 0.10628337666603471 1105 82-83 0.17856967439187343 1105 84-85 0.17496533089143895 1105 86-87 0.13283524382432432 1105 88-89 0.11986258151102902 1105 90-91 0.14134471706043428 1105 92-93 0.1658040578443405 1105 94-95 0.14003975896819298 1105 96-97 0.130567760677458 1105 98-99 0.1279322199738928 1105 100 0.1109314294662127 1106 1 0.031679480259747095 1106 2 0.05520398122155257 1106 3 0.04518666083342282 1106 4 0.05703396411227857 1106 5 0.06480988185929704 1106 6 0.08223233246877726 1106 7 0.09342362148283456 1106 8 0.10136907717037502 1106 9 0.07393778956538455 1106 10-11 0.08538681708980533 1106 12-13 0.09036635650126357 1106 14-15 0.09990568384878884 1106 16-17 0.141865560852672 1106 18-19 0.1395604071791965 1106 20-21 0.1207644560395238 1106 22-23 0.1407992400553013 1106 24-25 0.12426077186161422 1106 26-27 0.14890686762677063 1106 28-29 0.13002409658276548 1106 30-31 0.129633000965498 1106 32-33 0.09173175626298047 1106 34-35 0.08938256046178594 1106 36-37 0.08632530976135655 1106 38-39 0.10939303011326018 1106 40-41 0.13596224967689352 1106 42-43 0.1053983678551873 1106 44-45 0.07424588198085047 1106 46-47 0.051547108180812984 1106 48-49 0.06631713840319264 1106 50-51 0.047792575295588335 1106 52-53 0.06713822614054976 1106 54-55 0.07534365938874288 1106 56-57 0.018221186879330276 1106 58-59 0.04901123668427232 1106 60-61 0.057751056186326366 1106 62-63 0.02169341835409 1106 64-65 0.018754902286076458 1106 66-67 0.0383123773888272 1106 68-69 0.06611984413860483 1106 70-71 0.046221304000184205 1106 72-73 0.07723232896736221 1106 74-75 0.12082223677804649 1106 76-77 0.077227876581901 1106 78-79 0.0362645521913052 1106 80-81 0.06051729887554558 1106 82-83 0.07950703099463396 1106 84-85 0.08071168003867513 1106 86-87 0.02354141264309817 1106 88-89 0.035345443041258306 1106 90-91 0.045607201208568426 1106 92-93 0.06394714833773207 1106 94-95 0.04697788860308094 1106 96-97 -0.0026709765061099233 1106 98-99 0.016493757461418568 1106 100 0.024697639070588195 1107 1 0.005505947661944788 1107 2 0.04531589128751534 1107 3 0.06321537639315267 1107 4 0.04292010832820381 1107 5 0.05945870566193179 1107 6 0.04283644138127585 1107 7 0.06695514241935285 1107 8 0.015471706417578446 1107 9 0.051645940102091004 1107 10-11 0.07188726921423694 1107 12-13 0.06605896160528602 1107 14-15 0.09464546936067819 1107 16-17 0.0638483194455759 1107 18-19 0.0971895080006604 1107 20-21 0.04915724638462393 1107 22-23 0.07321694699290049 1107 24-25 0.08646741893869603 1107 26-27 0.10564122280925403 1107 28-29 0.09717298309536915 1107 30-31 0.10102998871992952 1107 32-33 0.05075678279946061 1107 34-35 0.06720391943503756 1107 36-37 0.05221480581752047 1107 38-39 0.09598072003824143 1107 40-41 0.08363579494565698 1107 42-43 0.11367762444564988 1107 44-45 0.1307082290080075 1107 46-47 0.12647666504728505 1107 48-49 0.10634249902747683 1107 50-51 0.11568500063316378 1107 52-53 0.11253143188685755 1107 54-55 0.12806909627738605 1107 56-57 0.08128009963083116 1107 58-59 0.10238893794477377 1107 60-61 0.13303616572953558 1107 62-63 0.11695129306684748 1107 64-65 0.0742200961850088 1107 66-67 0.0990663518633852 1107 68-69 0.07271237965872501 1107 70-71 0.10278787210847184 1107 72-73 0.09165913816956106 1107 74-75 0.11601156738949925 1107 76-77 0.06557504082381627 1107 78-79 0.061061278362920746 1107 80-81 0.012825063131685965 1107 82-83 0.10497511664569004 1107 84-85 0.0914857539477083 1107 86-87 0.11167315279693923 1107 88-89 0.04965905992771269 1107 90-91 0.05689608534432722 1107 92-93 0.08448145798212892 1107 94-95 0.12263529229135628 1107 96-97 0.0800469661371892 1107 98-99 0.09935593305335289 1107 100 0.09441954337238911 1108 1 -0.008428114842629952 1108 2 0.05349288068561009 1108 3 0.01236732086590564 1108 4 0.035522943119914885 1108 5 -0.0142727108814924 1108 6 0.019113521349154894 1108 7 -0.011868008361098248 1108 8 0.03410654944691771 1108 9 -0.0190506664227712 1108 10-11 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2214 54-55 -0.19243673930476746 2214 56-57 -0.18706220718223676 2214 58-59 -0.20768768008979777 2214 60-61 -0.2504809490089812 2214 62-63 -0.3289117202990042 2214 64-65 -0.25323399185773354 2214 66-67 -0.23633661589416732 2214 68-69 -0.27325647896489613 2214 70-71 -0.20123470966660761 2214 72-73 -0.20155727139069768 2214 74-75 -0.2776228507413663 2214 76-77 -0.25394799784398714 2214 78-79 -0.2121318411918196 2214 80-81 -0.24958319237277493 2214 82-83 -0.20671477081604905 2214 84-85 -0.173607096703698 2214 86-87 -0.20876102402480257 2214 88-89 -0.16212605764040688 2214 90-91 -0.13308374638709353 2214 92-93 -0.2027831996992262 2214 94-95 -0.21453005013733417 2214 96-97 -0.17819636074781897 2214 98-99 -0.24096244674129963 2214 100 -0.2580357696951552 2215 1 -0.19790411396688512 2215 2 -0.20963422070141746 2215 3 -0.21288106202913326 2215 4 -0.1351829404860112 2215 5 -0.14991367025215396 2215 6 -0.17158367718341339 2215 7 -0.17602117648847582 2215 8 -0.15649723166810503 2215 9 -0.19579499125251942 2215 10-11 -0.21526287361425034 2215 12-13 -0.2516307226188843 2215 14-15 -0.3457114845403382 2215 16-17 -0.3484218015494065 2215 18-19 -0.36446447831932005 2215 20-21 -0.3474687133708656 2215 22-23 -0.3507271613388596 2215 24-25 -0.38563255932835716 2215 26-27 -0.38818597734449867 2215 28-29 -0.4043590780317601 2215 30-31 -0.3966115813263684 2215 32-33 -0.35623024838481143 2215 34-35 -0.31546811864063784 2215 36-37 -0.34373563076891145 2215 38-39 -0.3473556451065747 2215 40-41 -0.32583718664167804 2215 42-43 -0.3308710229995526 2215 44-45 -0.3376771051067493 2215 46-47 -0.35518078234250083 2215 48-49 -0.3054450555353512 2215 50-51 -0.3421009119467584 2215 52-53 -0.30209649053824705 2215 54-55 -0.31722742438371654 2215 56-57 -0.2953682383572982 2215 58-59 -0.2754704951325593 2215 60-61 -0.18116619036789672 2215 62-63 -0.20410245882780487 2215 64-65 -0.18291385587624376 2215 66-67 -0.2141766677538115 2215 68-69 -0.1950758849960721 2215 70-71 -0.21498268214875793 2215 72-73 -0.21503773859903674 2215 74-75 -0.21958084261569866 2215 76-77 -0.183530360623962 2215 78-79 -0.25842919573380385 2215 80-81 -0.17351562513794505 2215 82-83 -0.23180513307649164 2215 84-85 -0.21192087175225538 2215 86-87 -0.20776871547680997 2215 88-89 -0.19638438418033388 2215 90-91 -0.18987099890408388 2215 92-93 -0.17491189665210527 2215 94-95 -0.15719576336566377 2215 96-97 -0.14841995592421142 2215 98-99 -0.186158442258467 2215 100 -0.19997796510429566 2216 1 -0.2022048025253511 2216 2 -0.15285108409860726 2216 3 -0.17479690403433068 2216 4 -0.13129950517603817 2216 5 -0.1776270739967103 2216 6 -0.1711309404697019 2216 7 -0.13497457754956343 2216 8 -0.1428514313799596 2216 9 -0.16169777431829147 2216 10-11 -0.2381619501302339 2216 12-13 -0.25348470166947834 2216 14-15 -0.2880091311918349 2216 16-17 -0.3088350128540256 2216 18-19 -0.3213104392688777 2216 20-21 -0.3376676593021841 2216 22-23 -0.34492518637429725 2216 24-25 -0.3252873082647625 2216 26-27 -0.3424051524160703 2216 28-29 -0.3208883180813089 2216 30-31 -0.30255259179382676 2216 32-33 -0.34620213165332814 2216 34-35 -0.339933761093441 2216 36-37 -0.2967671586008507 2216 38-39 -0.360451683119166 2216 40-41 -0.32379618323742676 2216 42-43 -0.3046134064634032 2216 44-45 -0.34631016424077643 2216 46-47 -0.2785820262157159 2216 48-49 -0.35840520083812066 2216 50-51 -0.39482215691667477 2216 52-53 -0.34403157456777933 2216 54-55 -0.26737995601225606 2216 56-57 -0.32458490801850814 2216 58-59 -0.2900009889115154 2216 60-61 -0.3238157821770642 2216 62-63 -0.36280211421176034 2216 64-65 -0.45676054674323296 2216 66-67 -0.45505125215015596 2216 68-69 -0.4336203638890339 2216 70-71 -0.37009543487266683 2216 72-73 -0.3974034471261305 2216 74-75 -0.44231559612015303 2216 76-77 -0.40164551263284665 2216 78-79 -0.4535608613514981 2216 80-81 -0.40414861096113697 2216 82-83 -0.3981215511212355 2216 84-85 -0.484318698773599 2216 86-87 -0.4812246056591576 2216 88-89 -0.6368031207814582 2216 90-91 -0.6039664061126473 2216 92-93 -0.5716514576293648 2216 94-95 -0.4863065676209075 2216 96-97 -0.5796609823892709 2216 98-99 -0.6791488969207222 2216 100 -0.6942174121365952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28.0 3 9.0 4 16.0 5 39.0 6 130.0 7 278.0 8 661.0 9 1350.0 10 2296.0 11 3565.0 12 5279.0 13 7562.0 14 8889.0 15 11203.0 16 12447.0 17 16608.0 18 18338.0 19 10319.0 20 8249.0 21 8268.0 22 8966.0 23 10158.0 24 12250.0 25 15300.0 26 19912.0 27 26171.0 28 33696.0 29 43799.0 30 56217.0 31 71544.0 32 92183.0 33 120385.0 34 167215.0 35 256643.0 36 481574.0 37 1115952.0 38 1929218.0 39 730135.0 40 993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.531128113871624 19.413870324515734 13.48185334487626 24.573148216736378 2 15.876532373171418 21.186134357452328 38.915716029066225 24.021617240310032 3 18.068601386256173 26.1659856091469 29.876818050533217 25.88859495406371 4 12.854058659295239 17.499704674396693 38.30553002137837 31.3407066449297 5 13.351677284617928 37.36717512884255 35.24122358739486 14.03992399914466 6 30.50009777985102 37.67427515739919 18.29274496113486 13.532882101614932 7 26.919663253165833 32.140444945170785 23.233553353573814 17.70633844808957 8 25.335631573662226 34.30979031163212 21.425219237469783 18.92935887723587 9 26.051325914754482 16.149096290490775 20.88103552383312 36.91854227092163 10-11 24.24198977695325 25.908609029221275 28.97360135845078 20.875799835374696 12-13 25.58479948076856 23.563376473879703 28.67880467496696 22.17301937038478 14-15 22.838797751664824 24.946246951560415 26.329050893315614 25.885904403459147 16-17 22.09997435868934 28.106126389241876 26.600495380325068 23.19340387174371 18-19 21.944258084158484 27.001768523003516 28.901673173105564 22.15230021973244 20-21 23.37971656856187 26.44547141499718 28.171332072746292 22.00347994369466 22-23 22.77171902070825 26.32314751247024 28.21808171917897 22.687051747642542 24-25 22.71899028133349 26.394786290414885 28.193543604095538 22.69267982415609 26-27 22.485501853063024 26.42365288963944 28.188676227315725 22.902169029981817 28-29 22.445378828870243 26.475027845643943 28.26341338610566 22.816179939380145 30-31 22.791811472104335 26.846822168073814 27.80594104137078 22.55542531845107 32-33 22.476607738168692 26.52819552944745 28.29763303186133 22.697563700522526 34-35 22.680466366293665 26.29122553503352 28.250862638227005 22.77744546044581 36-37 23.06317347247329 26.619965353170638 27.563012861151748 22.753848313204323 38-39 22.888328502433662 26.790316974214583 27.677136389627048 22.644218133724703 40-41 22.71406284986575 26.514319055804155 27.86414321683992 22.907474877490174 42-43 22.70939524420928 26.345531943754953 27.771336578215827 23.173736233819938 44-45 22.830310606281834 26.520659514360347 27.68956139450191 22.959468484855908 46-47 22.948569523036184 26.67960349256619 27.48167099830534 22.890155986092285 48-49 22.939036463951002 26.608425830068512 27.273865382278494 23.178672323701992 50-51 22.879671505102355 26.572158757461832 27.169237232812943 23.378932504622874 52-53 22.935720617312676 26.476065145082423 27.205777485966525 23.382436751638377 54-55 23.038558963195044 26.877602868960942 27.076638447430174 23.00719972041384 56-57 22.83023524613096 26.581691816547014 27.160768635858805 23.427304301463213 58-59 22.678648302653905 26.2010665345352 27.506511588036197 23.613773574774697 60-61 22.958724303366054 26.687601088577377 27.074264602677733 23.279410005378832 62-63 22.848839783377244 26.568701610540625 27.074245762640015 23.508212843442113 64-65 22.79481597522158 26.276219444991327 27.265133024796317 23.66383155499077 66-67 22.66871018275779 26.417990728817436 27.100179074558504 23.813120013866268 68-69 23.124742832855297 26.462687590715007 27.020476533545146 23.39209304288455 70-71 23.054708486047627 26.30233718336002 27.058444844439606 23.58450948615275 72-73 22.884946807333133 26.45997821142454 27.086053568997865 23.569021412244464 74-75 22.903242914458414 26.504100024991363 26.917668008990862 23.67498905155936 76-77 22.966712479358385 26.430104873069954 26.882473018710982 23.72070962886068 78-79 23.0723579908607 26.613588000403176 26.847213888122205 23.46684012061392 80-81 22.972157250258814 26.43332651951969 26.97087047568269 23.6236457545388 82-83 22.757692588844115 26.43530244717433 27.083466783826783 23.723538180154772 84-85 22.70558013657143 26.399018810835663 26.915348884528466 23.980052168064443 86-87 22.834381017361462 26.540526960369874 26.766674482181337 23.858417540087327 88-89 22.59011646254556 26.449263006774103 26.87451191331863 24.08610861736171 90-91 22.490867777131655 26.33199229838293 26.993463196461224 24.18367672802419 92-93 22.632685658561876 26.505734570655154 26.763713005444057 24.097866765338914 94-95 22.34785256146977 26.251353665126043 26.94120477515029 24.459588998253896 96-97 22.337616754420687 26.261273677053275 26.94422710302084 24.4568824655052 98-99 22.30859663120651 26.412388478541626 26.826785308887715 24.452229581364154 100 22.380966286694505 26.303254899116308 26.7201472537348 24.595631560454382 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 32.0 2 128.0 3 204.5 4 134.0 5 116.0 6 253.0 7 571.5 8 959.5 9 1027.0 10 789.0 11 633.5 12 687.0 13 831.0 14 972.5 15 1122.5 16 1251.5 17 1404.5 18 1646.5 19 1892.0 20 2174.0 21 2585.5 22 3194.0 23 4194.5 24 5523.0 25 7177.5 26 9837.0 27 13931.5 28 19809.0 29 27398.5 30 36459.5 31 47962.0 32 62915.0 33 81280.5 34 101027.0 35 120228.5 36 138802.0 37 158400.5 38 175662.0 39 187149.0 40 199361.5 41 209791.0 42 213178.0 43 215059.0 44 215387.0 45 212160.5 46 209176.0 47 205556.5 48 201288.5 49 198667.5 50 194675.5 51 187595.0 52 177005.5 53 168731.5 54 162567.0 55 153632.0 56 139351.0 57 123992.5 58 112897.0 59 102909.0 60 91202.5 61 78170.0 62 66905.0 63 56851.5 64 47237.5 65 37175.0 66 28620.0 67 21474.0 68 15731.0 69 12093.0 70 8636.5 71 6063.0 72 4465.0 73 3234.5 74 2361.0 75 1635.0 76 1005.0 77 647.0 78 465.0 79 249.0 80 128.5 81 70.0 82 29.0 83 11.0 84 8.0 85 5.5 86 2.5 87 2.5 88 4.5 89 4.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0458189717295814 2 0.23789315626209884 3 0.003165126336582926 4 0.0027129654313567935 5 1.884003771775551E-4 6 5.6520113153266536E-5 7 0.0 8 3.0144060348408816E-4 9 0.0 10-11 6.405612824036873E-4 12-13 0.0 14-15 1.2246024516541083E-4 16-17 7.536015087102204E-5 18-19 4.898409806616433E-4 20-21 9.420018858877755E-6 22-23 1.695603394597996E-4 24-25 0.001309382621384008 26-27 2.1666043375418839E-4 28-29 2.4492049033082166E-4 30-31 8.47801697298998E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.036202074476553E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4492049033082166E-4 70-71 5.6520113153266536E-5 72-73 3.2970066006072146E-4 74-75 2.1666043375418839E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.695603394597996E-4 84-85 0.0 86-87 4.99260999520521E-4 88-89 0.0024492049033082164 90-91 0.0024209448467315832 92-93 6.97081395556954E-4 94-95 6.217212446859319E-4 96-97 3.0144060348408816E-4 98-99 1.884003771775551E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5307845.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.659716552593274 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38316378441472 37.06357454962027 2 15.862446795194154 16.706239041310315 3 5.736226024016899 9.062041095190168 4 2.7160980299446544 5.7211580954376995 5 1.5446329808613455 4.066996747497285 6 0.9115625315468013 2.8801574718731353 7 0.5813225895506982 2.1428597953951494 8 0.40130130810850895 1.6905930509743186 9 0.30565212370331235 1.4485998778123144 >10 1.4455933220084423 13.480261836733407 >50 0.08324094567858596 2.986104316197718 >100 0.028115255006531725 2.3837922861783007 >500 5.369254695646984E-4 0.1826709634086909 >1k 1.0738449575846176E-4 0.1849508723712872 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 3.768007543551102E-5 0.0 0.0 7.536015087102204E-5 0.0 10-11 3.768007543551102E-5 0.0 0.0 7.536015087102204E-5 0.0 12-13 3.768007543551102E-5 0.0 0.0 7.536015087102204E-5 0.0 14-15 3.768007543551102E-5 0.0 0.0 1.7898035831867738E-4 0.0 16-17 3.768007543551102E-5 0.0 0.0 2.4492049033082166E-4 0.0 18-19 5.652011315326653E-5 0.0 0.0 2.4492049033082166E-4 0.0 20-21 7.536015087102204E-5 0.0 0.0 2.5434050918969945E-4 0.0 22-23 7.536015087102204E-5 0.0 0.0 2.826005657663327E-4 0.0 24-25 7.536015087102204E-5 0.0 0.0 3.768007543551102E-4 0.0 26-27 9.420018858877756E-5 0.0 0.0 7.441814898513427E-4 0.0 28-29 1.1304022630653307E-4 0.0 0.0 0.0016485033003036072 0.0 30-31 1.1304022630653307E-4 0.0 0.0 0.006433872880613508 0.0 32-33 1.1304022630653307E-4 0.0 0.0 0.01976319956592553 0.0 34-35 1.1304022630653307E-4 0.0 0.0 0.0388764178305885 0.0 36-37 1.1304022630653307E-4 0.0 0.0 0.06985885985743744 0.0 38-39 1.1304022630653307E-4 0.0 0.0 0.1240804884091378 0.0 40-41 1.1304022630653307E-4 0.0 0.0 0.19571973183090313 0.0 42-43 1.1304022630653307E-4 0.0 0.0 0.26888501830780664 0.0 44-45 1.1304022630653307E-4 0.0 0.0 0.35323186717019806 0.0 46-47 1.318802640242886E-4 0.0 0.0 0.45037110164294547 0.0 48-49 1.4130028288316635E-4 0.0 0.0 0.560217941556319 0.0 50-51 1.5072030174204408E-4 0.0 0.0 0.6861541736806558 0.0 52-53 2.0724041489531063E-4 0.0 0.0 0.8141439699162278 0.0 54-55 2.2608045261306614E-4 0.0 0.0 0.9483698186363769 0.0 56-57 2.2608045261306614E-4 0.0 0.0 1.1021516265075562 0.0 58-59 2.2608045261306614E-4 0.0 0.0 1.2755930137372136 0.0 60-61 2.2608045261306614E-4 0.0 0.0 1.4677425584206019 0.0 62-63 2.637605280485772E-4 0.0 0.0 1.6689824213028075 0.0 64-65 2.637605280485772E-4 0.0 0.0 1.882609608984437 0.0 66-67 2.637605280485772E-4 0.0 0.0 2.1180158802677926 0.0 68-69 2.8260056576633264E-4 0.0 0.0 2.380203265166937 0.0 70-71 2.8260056576633264E-4 0.0 0.0 2.6709898273216344 0.0 72-73 2.8260056576633264E-4 0.0 0.0 2.9843844347376383 0.0 74-75 3.0144060348408816E-4 0.0 0.0 3.304919416448672 0.0 76-77 3.0144060348408816E-4 0.0 0.0 3.652989113284205 0.0 78-79 3.0144060348408816E-4 0.0 0.0 4.0320789322220225 0.0 80-81 3.0144060348408816E-4 0.0 0.0 4.447812624520875 0.0 82-83 3.0144060348408816E-4 0.0 0.0 4.892267954320444 0.0 84-85 3.2028064120184367E-4 0.0 0.0 5.348027306750668 0.0 86-87 3.2028064120184367E-4 0.0 0.0 5.820752113145731 0.0 88 3.2028064120184367E-4 1.884003771775551E-5 0.0 6.198579649556458 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11130 0.0 47.725216 1 GGTATCA 5800 0.0 47.61993 1 TCAACGC 13535 0.0 38.78835 4 ATCAACG 13890 0.0 37.899586 3 CAACGCA 14075 0.0 37.231308 5 TATCAAC 14325 0.0 36.80014 2 AACGCAG 15090 0.0 34.85127 6 ACGCAGA 17405 0.0 30.026766 7 CGCAGAG 17725 0.0 29.166765 8 GTACATG 16575 0.0 25.165693 1 GCAGAGT 20145 0.0 25.033081 9 TACATGG 16515 0.0 24.275324 2 ACATGGG 18085 0.0 21.571394 3 CATGGGG 12315 0.0 20.0751 4 AGAGTAC 18450 0.0 19.919945 10-11 CAGAGTA 19590 0.0 18.892693 10-11 GAGTACT 11560 0.0 18.274673 12-13 ATTATAC 2165 0.0 17.80224 3 AGTACTT 12185 0.0 17.260178 12-13 GTACTTT 12185 0.0 17.028835 14-15 >>END_MODULE