FastQCFastQC Report
Wed 25 May 2016
SRR1033067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4120930
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48750.035.3918341
TCAACGC47000.035.007894
ATCAACG49300.033.2793053
CAACGCA49800.033.038775
AACGCAG51650.031.9382536
GGTATCA29550.031.023351
TATCAAC60400.027.63042
ACGCAGA62700.025.8604767
GTACATG103100.025.2617071
CGCAGAG67850.023.9214258
TACATGG106400.023.4169312
ACATGGG108200.022.1149653
GTATACG3101.2732926E-1121.2313651
CATGGGG77950.019.359084
GCAGAGT84250.018.4311149
GTATAGG18450.017.8366491
GTATTAG17700.017.2644061
AGAGTAC67350.017.15998610-11
GAGTACA79150.017.1061861
AGTACAT79100.015.4522812
Produced by FastQC (version 0.11.5)