FastQCFastQC Report
Wed 25 May 2016
SRR1033067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4120930
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48860.11856546944500392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53500.049.3379361
GTATCAA96100.041.4457551
TCAACGC110000.035.5489434
ATCAACG110100.035.4752543
CAACGCA112600.034.768575
AACGCAG115700.033.9588626
TATCAAC119950.032.995252
ACGCAGA135450.029.007317
CGCAGAG138600.028.2124168
GCAGAGT158250.024.1446979
GTACATG99800.023.7565941
TACATGG101700.022.7474582
ACATGGG105700.021.2996433
GAGTACT103450.021.03404412-13
CAGAGTA153800.020.16798610-11
AGAGTAC142000.020.08978310-11
GTGGTAT26400.018.3899271
GTACTTT119150.018.20340214-15
CATGGGG77900.017.7983844
AGTACTT112600.017.78048112-13
Produced by FastQC (version 0.11.5)