FastQCFastQC Report
Wed 25 May 2016
SRR1033066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873060
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31500.037.0095021
ATCAACG30350.035.780643
TCAACGC30250.035.588114
GGTATCA20050.035.4025651
CAACGCA30850.034.8959585
AACGCAG34850.030.8842266
TATCAAC38850.028.3152032
GTACATG70300.027.549521
TACATGG71550.026.1497942
CGCAGAG40750.025.6289318
TATACCG1501.1612847E-625.0722665
ACGCAGA41950.024.9857337
ACATGGG75350.023.5208453
TCTACGC2206.3004336E-821.3684063
CTGTCGC7000.020.164929
GAGTACA53500.019.506141
CATGGGG53300.019.1393594
GCAGAGT54050.018.9772859
TCTGTCG7450.018.9439338
AGTACAT54150.017.9707722
Produced by FastQC (version 0.11.5)