##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033066_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2873060 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.645129931153544 33.0 31.0 34.0 30.0 34.0 2 31.76036212261491 34.0 31.0 34.0 30.0 34.0 3 31.86883705874573 34.0 31.0 34.0 30.0 34.0 4 35.229642262953085 37.0 35.0 37.0 33.0 37.0 5 35.239202801194544 37.0 35.0 37.0 33.0 37.0 6 35.349809262598065 37.0 35.0 37.0 33.0 37.0 7 35.26295691701531 37.0 35.0 37.0 33.0 37.0 8 35.254723883246434 37.0 36.0 37.0 33.0 37.0 9 36.896361718864206 39.0 38.0 39.0 34.0 39.0 10-11 36.88592284880929 39.0 38.0 39.0 34.0 39.0 12-13 36.81188262688562 39.0 38.0 39.0 33.0 39.0 14-15 38.21256882905334 41.0 39.0 41.0 34.0 41.0 16-17 38.21807010643704 41.0 39.0 41.0 33.5 41.0 18-19 38.23418028165092 41.0 39.0 41.0 33.5 41.0 20-21 38.25677796495722 41.0 39.0 41.0 34.0 41.0 22-23 38.22395042219794 40.0 39.0 41.0 34.0 41.0 24-25 38.23452625423764 40.0 39.0 41.0 34.0 41.0 26-27 38.14644020660898 40.0 38.0 41.0 33.0 41.0 28-29 38.15553486526561 40.0 38.0 41.0 33.0 41.0 30-31 38.30380465427106 40.5 39.0 41.0 34.0 41.0 32-33 38.28813303585724 41.0 39.0 41.0 33.5 41.0 34-35 38.245301351172614 41.0 38.5 41.0 33.0 41.0 36-37 38.1719295803081 41.0 38.0 41.0 33.0 41.0 38-39 38.089721412013674 40.5 38.0 41.0 33.0 41.0 40-41 38.02144229497469 40.0 38.0 41.0 33.0 41.0 42-43 37.906632127418156 40.0 38.0 41.0 33.0 41.0 44-45 37.783420812652714 40.0 38.0 41.0 32.5 41.0 46-47 37.66239549469903 40.0 38.0 41.0 32.0 41.0 48-49 37.55236020131845 40.0 38.0 41.0 32.0 41.0 50-51 37.43786102622292 40.0 37.5 41.0 32.0 41.0 52-53 37.28809405303056 40.0 37.0 41.0 31.0 41.0 54-55 37.14154890604442 40.0 37.0 41.0 31.0 41.0 56-57 36.97868561742532 40.0 36.5 41.0 31.0 41.0 58-59 36.78939458277934 40.0 36.0 41.0 31.0 41.0 60-61 36.5776423047204 39.0 35.5 41.0 31.0 41.0 62-63 36.33451006940335 39.0 35.0 41.0 30.0 41.0 64-65 36.063005819579125 39.0 35.0 41.0 30.0 41.0 66-67 35.77584335168775 38.0 35.0 40.0 30.0 41.0 68-69 35.464031729236424 37.5 35.0 40.0 29.5 41.0 70-71 35.132002464271544 37.0 35.0 40.0 29.0 41.0 72-73 34.77747714979847 36.5 34.0 39.0 29.0 41.0 74-75 34.418236653602776 36.0 34.0 39.0 29.0 41.0 76-77 34.04825760687211 35.5 34.0 38.5 28.5 40.0 78-79 33.65804629906789 35.0 34.0 37.5 28.0 39.5 80-81 33.29606082017083 35.0 34.0 37.0 27.5 39.0 82-83 33.05932472694619 35.0 34.0 36.5 27.5 39.0 84-85 32.82559483616771 35.0 34.0 36.0 27.5 37.5 86-87 32.638658955956366 35.0 34.0 36.0 27.5 37.0 88-89 32.40788009996311 35.0 34.0 35.5 27.0 37.0 90-91 32.19141124793774 35.0 33.5 35.0 27.0 36.0 92-93 31.9694263259382 35.0 33.0 35.0 26.5 36.0 94-95 31.767816195972237 35.0 33.0 35.0 26.0 36.0 96-97 31.596081529797498 35.0 33.0 35.0 25.5 36.0 98-99 31.42723124473558 35.0 33.0 35.0 25.0 35.5 100 31.288414791198235 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16745597784129984 1101 2 0.19222479337148357 1101 3 0.1540330394645224 1101 4 0.09140908677139237 1101 5 0.050994991840738635 1101 6 0.12221279533671492 1101 7 0.19465269645441197 1101 8 0.23859542907729292 1101 9 0.2545617314613082 1101 10-11 0.20858936432757247 1101 12-13 0.26663304113188957 1101 14-15 0.340406612005026 1101 16-17 0.29084787388854494 1101 18-19 0.2986798314727963 1101 20-21 0.3068524485389901 1101 22-23 0.3468894437632102 1101 24-25 0.2801852398195024 1101 26-27 0.31223963307267866 1101 28-29 0.3467755590743167 1101 30-31 0.3395901035536468 1101 32-33 0.31216564093487875 1101 34-35 0.3667201310285151 1101 36-37 0.3714536912590134 1101 38-39 0.36437367210521643 1101 40-41 0.39690543343527196 1101 42-43 0.397504190630535 1101 44-45 0.337203932969075 1101 46-47 0.32006525212031534 1101 48-49 0.3064585060694469 1101 50-51 0.27136218267039425 1101 52-53 0.22519754755087007 1101 54-55 0.2568279010854795 1101 56-57 0.34690765157804293 1101 58-59 0.361620068969998 1101 60-61 0.3514776437920233 1101 62-63 0.37077659027183785 1101 64-65 0.3241991732673455 1101 66-67 0.32181733239286103 1101 68-69 0.253713322855333 1101 70-71 0.24107375542601517 1101 72-73 0.25043543041035576 1101 74-75 0.32281363285287057 1101 76-77 0.2767033256751006 1101 78-79 0.22155741837638487 1101 80-81 0.259506611114638 1101 82-83 0.29114866066187517 1101 84-85 0.3161247139006491 1101 86-87 0.2129845053210886 1101 88-89 0.3039275887268573 1101 90-91 0.42652653472264035 1101 92-93 0.37673128055318017 1101 94-95 0.3453848375783508 1101 96-97 0.31432543851029493 1101 98-99 0.293569025749143 1101 100 0.30001523335590363 1102 1 -0.025485654969529037 1102 2 0.057919844878302484 1102 3 0.05929508768171843 1102 4 -0.07509850477451607 1102 5 -0.05417042195730204 1102 6 -0.029542437112986875 1102 7 -0.02597854930516519 1102 8 0.05077974537864094 1102 9 0.11077201336350839 1102 10-11 0.08686581174898578 1102 12-13 0.09682703979657958 1102 14-15 0.14763371775686096 1102 16-17 0.08857270771788706 1102 18-19 0.14106997232928364 1102 20-21 0.2159362866688994 1102 22-23 0.1781195931613766 1102 24-25 0.10642506617487868 1102 26-27 -0.007255043249323023 1102 28-29 0.006258368959947802 1102 30-31 0.12721184264882623 1102 32-33 0.05960151757800247 1102 34-35 0.109432814600126 1102 36-37 0.1380973819099438 1102 38-39 0.23592794550899043 1102 40-41 0.2108748510067855 1102 42-43 0.1706644875347152 1102 44-45 0.1062394268588065 1102 46-47 0.08566562892697505 1102 48-49 0.0732956007117096 1102 50-51 0.07397164987010285 1102 52-53 -0.0043590694065045454 1102 54-55 0.05235859298792178 1102 56-57 0.0944686799304506 1102 58-59 0.018880016909051278 1102 60-61 5.822549432323854E-4 1102 62-63 -0.00459203962780208 1102 64-65 -0.02077556663063973 1102 66-67 0.06776470109844723 1102 68-69 0.06940229247564389 1102 70-71 0.02461860325720977 1102 72-73 -0.037827407100557764 1102 74-75 -0.038009557245281655 1102 76-77 0.02646532118261291 1102 78-79 -0.02682736771690486 1102 80-81 0.05994964275937065 1102 82-83 1.820867101898216E-4 1102 84-85 -0.02872757734872522 1102 86-87 -0.0047616286263689744 1102 88-89 -0.017231835088729497 1102 90-91 0.04783131562605547 1102 92-93 0.04593247206233286 1102 94-95 0.059715046173536734 1102 96-97 0.050705053648513854 1102 98-99 0.12326870219278163 1102 100 0.00922996719903324 1103 1 0.17858330871038675 1103 2 0.21490893477493245 1103 3 0.24707862408472892 1103 4 0.2328703161136616 1103 5 0.10040685365372326 1103 6 0.21720321991050895 1103 7 0.24953982491156523 1103 8 0.2776131773976829 1103 9 0.35163851393172507 1103 10-11 0.3470198398105495 1103 12-13 0.3375626196467252 1103 14-15 0.38222865884972634 1103 16-17 0.4089970841239037 1103 18-19 0.3847137877912772 1103 20-21 0.35826157052198937 1103 22-23 0.31775372166558213 1103 24-25 0.27384954940697526 1103 26-27 0.2167659440274008 1103 28-29 0.20753754599688534 1103 30-31 0.2146486644240042 1103 32-33 0.22712936614510681 1103 34-35 0.19773292954916855 1103 36-37 0.2509807510739748 1103 38-39 0.25088323787943523 1103 40-41 0.2712938808849188 1103 42-43 0.2557304036676271 1103 44-45 0.29972287913301443 1103 46-47 0.3480817776351586 1103 48-49 0.30828203066501203 1103 50-51 0.30591735636269846 1103 52-53 0.2699432113172904 1103 54-55 0.30947810081482174 1103 56-57 0.3276127517489158 1103 58-59 0.3004154224420148 1103 60-61 0.25700905831245535 1103 62-63 0.26810602154410645 1103 64-65 0.29566659984410393 1103 66-67 0.2726145856400919 1103 68-69 0.32421658355921323 1103 70-71 0.26344261002930836 1103 72-73 0.269002338243304 1103 74-75 0.20847602136422694 1103 76-77 0.2906539927598004 1103 78-79 0.23126457741366124 1103 80-81 0.24519612500782273 1103 82-83 0.2179735332058641 1103 84-85 0.21957476292604383 1103 86-87 0.214853860163025 1103 88-89 0.22714778092765897 1103 90-91 0.17605581782042634 1103 92-93 0.21043451755978992 1103 94-95 0.19485288055027894 1103 96-97 0.18996920869207656 1103 98-99 0.16175185148836846 1103 100 0.14261852448014523 1104 1 -0.03581563393261433 1104 2 0.05268977410899467 1104 3 0.08247838276034258 1104 4 0.06786008739113214 1104 5 -0.03857956263529161 1104 6 -0.007218888397119372 1104 7 0.04587595675930345 1104 8 0.041371830731748105 1104 9 0.1220023946426636 1104 10-11 0.15024869706642363 1104 12-13 0.17164187098671135 1104 14-15 0.1602685398857986 1104 16-17 0.1899842188920502 1104 18-19 0.16681862629960875 1104 20-21 0.18783562227439177 1104 22-23 0.17114871705353352 1104 24-25 0.10802735540997332 1104 26-27 0.11754764832724618 1104 28-29 0.08571531595703874 1104 30-31 0.11696838463639381 1104 32-33 0.08385444537836406 1104 34-35 0.1598068112556632 1104 36-37 0.2287866476964524 1104 38-39 0.15025260692748077 1104 40-41 0.27646446524023105 1104 42-43 0.3265089523410225 1104 44-45 0.27898022000054823 1104 46-47 0.2460154086098214 1104 48-49 0.22822667235436 1104 50-51 0.19697099581745192 1104 52-53 0.23539423280977445 1104 54-55 0.26066194255567865 1104 56-57 0.18309529133551194 1104 58-59 0.27089423353362463 1104 60-61 0.268550153510688 1104 62-63 0.29274991225943126 1104 64-65 0.21541322487876613 1104 66-67 0.22103138788981624 1104 68-69 0.2752273758266952 1104 70-71 0.24299602161942602 1104 72-73 0.2718645846676253 1104 74-75 0.34428669353894037 1104 76-77 0.1928710909738669 1104 78-79 0.2601956585445535 1104 80-81 0.2878897860157039 1104 82-83 0.24332708006929238 1104 84-85 0.30850529715676345 1104 86-87 0.28309802092439185 1104 88-89 0.21014947938430595 1104 90-91 0.25295276512258624 1104 92-93 0.25359298113076534 1104 94-95 0.2517569037643739 1104 96-97 0.2522090126490184 1104 98-99 0.301604485047303 1104 100 0.31553052192410647 1105 1 0.06833703731344087 1105 2 0.10792955967064444 1105 3 0.11621150465042263 1105 4 0.1367086232335737 1105 5 0.04932290452006782 1105 6 0.1298486024139507 1105 7 0.14286638151128273 1105 8 0.18363124790865726 1105 9 0.2320093099232423 1105 10-11 0.22785394351101473 1105 12-13 0.21262957241349767 1105 14-15 0.23231070581797297 1105 16-17 0.26299015136350334 1105 18-19 0.23340165251243405 1105 20-21 0.2329307335365911 1105 22-23 0.3061660146587428 1105 24-25 0.20851600350024313 1105 26-27 0.20065200041671005 1105 28-29 0.20585362274417918 1105 30-31 0.2655028929209138 1105 32-33 0.2044144808399011 1105 34-35 0.18680843704600392 1105 36-37 0.23002826557824108 1105 38-39 0.2829910239500464 1105 40-41 0.3152301199814218 1105 42-43 0.2984524612105801 1105 44-45 0.2711858066021904 1105 46-47 0.25708688096744936 1105 48-49 0.2344949163585639 1105 50-51 0.26180501321462657 1105 52-53 0.3175646164838355 1105 54-55 0.27707688854231804 1105 56-57 0.32289521721047265 1105 58-59 0.2680989696838765 1105 60-61 0.17543968545916044 1105 62-63 0.22430232868745037 1105 64-65 0.2126171675878581 1105 66-67 0.19697898292261584 1105 68-69 0.26484073135757313 1105 70-71 0.18976173207781244 1105 72-73 0.15165354577337808 1105 74-75 0.19881745419511532 1105 76-77 0.2035614470902516 1105 78-79 0.21617233936738245 1105 80-81 0.23738296518400404 1105 82-83 0.1788548424582359 1105 84-85 0.19019072958866445 1105 86-87 0.16233721546053204 1105 88-89 0.19515094343019257 1105 90-91 0.1551403353200982 1105 92-93 0.1964937221107732 1105 94-95 0.15847443529954575 1105 96-97 0.23053131832557838 1105 98-99 0.2747272825868592 1105 100 0.282841451064872 1106 1 -0.15856438784992832 1106 2 -0.04616556291793472 1106 3 -0.05039646165194256 1106 4 -0.060435161475567156 1106 5 -0.09209149501631231 1106 6 0.015581214069818827 1106 7 -0.030183996067236762 1106 8 0.03231396729173497 1106 9 0.017372187057347332 1106 10-11 0.06215586989031152 1106 12-13 0.07899856342201161 1106 14-15 0.01905128235406295 1106 16-17 0.05104804184795597 1106 18-19 0.0942000159833114 1106 20-21 0.09603602132466449 1106 22-23 0.09334717724149044 1106 24-25 0.00847538358884492 1106 26-27 -0.019671798940485985 1106 28-29 -0.019670936303128883 1106 30-31 0.031012788357969612 1106 32-33 -0.07042760592138819 1106 34-35 -0.0382512595579243 1106 36-37 -0.038836883426611735 1106 38-39 -0.01302513601952171 1106 40-41 -0.014631003474669058 1106 42-43 0.014859650046055606 1106 44-45 -0.007061697539796796 1106 46-47 -0.07198134315135007 1106 48-49 -0.0894138790521879 1106 50-51 -0.13607840719524233 1106 52-53 -0.06235160383784688 1106 54-55 -0.12295257644811386 1106 56-57 -0.0946737499771686 1106 58-59 -0.08160530269529431 1106 60-61 -0.12825692646691778 1106 62-63 -0.1200349156826448 1106 64-65 -0.06653784048280187 1106 66-67 -0.06389112091148519 1106 68-69 0.00928193648012865 1106 70-71 -0.020738737228825244 1106 72-73 -0.03962907237101376 1106 74-75 0.012929306943050278 1106 76-77 0.004038353292919794 1106 78-79 0.027416410815767733 1106 80-81 0.04652415626291173 1106 82-83 0.05978150529696791 1106 84-85 -0.00800488417196732 1106 86-87 0.041213439390901385 1106 88-89 0.11123027313943368 1106 90-91 0.10262965592763607 1106 92-93 0.09110643969840027 1106 94-95 0.045043777132544705 1106 96-97 0.01906729272347718 1106 98-99 0.06725736309783414 1106 100 0.11407216327324576 1107 1 0.00466788613288216 1107 2 0.025531074428997158 1107 3 0.07666930177781595 1107 4 0.10693504914107166 1107 5 0.06398501375909404 1107 6 0.0833621202922572 1107 7 0.16144753079228025 1107 8 0.17971068018854197 1107 9 0.22197431954652558 1107 10-11 0.1739621506999569 1107 12-13 0.1910343141518709 1107 14-15 0.15131697519083787 1107 16-17 0.14962753971478548 1107 18-19 0.1745971741457808 1107 20-21 0.14183092093905003 1107 22-23 0.19214279133205991 1107 24-25 0.13710545243836236 1107 26-27 0.14249111102867573 1107 28-29 0.1627267231036882 1107 30-31 0.1327067421460697 1107 32-33 0.07417108024580443 1107 34-35 0.06565914077094703 1107 36-37 0.1104600070636721 1107 38-39 0.09671244354714759 1107 40-41 0.055511904580107796 1107 42-43 0.09478339296111926 1107 44-45 0.12387311249158017 1107 46-47 0.1678481077104621 1107 48-49 0.10795215239160427 1107 50-51 0.09254291231235356 1107 52-53 0.08427310515839537 1107 54-55 0.038580359536190656 1107 56-57 0.129338941803276 1107 58-59 0.10590789075681784 1107 60-61 0.14229676265731683 1107 62-63 0.14551920289647313 1107 64-65 0.08907972845218382 1107 66-67 0.1278904480816223 1107 68-69 0.14858769809805494 1107 70-71 0.1169333865772515 1107 72-73 0.11869956402214399 1107 74-75 0.1572618806469066 1107 76-77 0.11189797581703687 1107 78-79 0.057365012972184104 1107 80-81 0.1597180997953629 1107 82-83 0.1529818671500962 1107 84-85 0.11560440846243125 1107 86-87 0.08807999985472748 1107 88-89 0.06879040964199135 1107 90-91 0.06720870895612308 1107 92-93 0.07977027310238327 1107 94-95 0.14853492480690988 1107 96-97 0.117304615471852 1107 98-99 0.08506216709547942 1107 100 0.10391160270002331 1108 1 -0.07324946029472201 1108 2 0.035608742847433206 1108 3 -0.025916025606797177 1108 4 0.03787463485318199 1108 5 -0.022025789468479218 1108 6 0.02392396807979935 1108 7 -0.05744332190766954 1108 8 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-0.3178923352134646 2214 58-59 -0.22344019735655252 2214 60-61 -0.1395761565647078 2214 62-63 -0.1444220762895796 2214 64-65 -0.13008061046422625 2214 66-67 -0.17652905206610825 2214 68-69 -0.2209090097128268 2214 70-71 -0.16935633578997766 2214 72-73 -0.1151244316640927 2214 74-75 -0.0770394484665573 2214 76-77 -0.11124627257518682 2214 78-79 -0.11955520104825013 2214 80-81 -0.18513824812463042 2214 82-83 -0.15649424258539568 2214 84-85 -0.19787584952409532 2214 86-87 -0.15987126877653424 2214 88-89 -0.20864911469141134 2214 90-91 -0.24580409860909924 2214 92-93 -0.21789631993326353 2214 94-95 -0.149068317935658 2214 96-97 -0.20936587592560585 2214 98-99 -0.23831570745220887 2214 100 -0.25977027310980816 2215 1 -0.19118956608049942 2215 2 -0.30423301169102146 2215 3 -0.2933854602260979 2215 4 -0.28884015595616575 2215 5 -0.15185119995847884 2215 6 -0.22931226441602348 2215 7 -0.267799127267935 2215 8 -0.24244606823939563 2215 9 -0.3761652298642133 2215 10-11 -0.32390358869120206 2215 12-13 -0.3977040775526177 2215 14-15 -0.43880868590939315 2215 16-17 -0.4920066792499398 2215 18-19 -0.4331777810868047 2215 20-21 -0.42166072941700605 2215 22-23 -0.4459542810870474 2215 24-25 -0.3511795390245922 2215 26-27 -0.3953139320729804 2215 28-29 -0.4325602048103576 2215 30-31 -0.46019606050249706 2215 32-33 -0.3705828030673999 2215 34-35 -0.45109740776526763 2215 36-37 -0.40972176947403227 2215 38-39 -0.40462537346863314 2215 40-41 -0.4008476714828575 2215 42-43 -0.41918300024256183 2215 44-45 -0.42801284511471493 2215 46-47 -0.40235344977922693 2215 48-49 -0.37893255589141006 2215 50-51 -0.37297576507073416 2215 52-53 -0.3561497907729745 2215 54-55 -0.3819913099761578 2215 56-57 -0.3961394449039233 2215 58-59 -0.3756151384357054 2215 60-61 -0.5099150601053566 2215 62-63 -0.4882468821407997 2215 64-65 -0.5833971590670757 2215 66-67 -0.5537429380009158 2215 68-69 -0.5080279892379664 2215 70-71 -0.5729143000924708 2215 72-73 -0.5546778773637655 2215 74-75 -0.5550449947955585 2215 76-77 -0.4504718863409636 2215 78-79 -0.5635616725731438 2215 80-81 -0.5685046337239825 2215 82-83 -0.5340730614302629 2215 84-85 -0.5259465222834194 2215 86-87 -0.5352776605791973 2215 88-89 -0.628303352070418 2215 90-91 -0.6714771070601984 2215 92-93 -0.6397228139754496 2215 94-95 -0.7401373568738485 2215 96-97 -0.6411413304740918 2215 98-99 -0.5512233865629526 2215 100 -0.5757671129956421 2216 1 -0.3616764100219818 2216 2 -0.42450987950029173 2216 3 -0.38335713970425545 2216 4 -0.46282016398403414 2216 5 -0.25915594517448426 2216 6 -0.46365735324765467 2216 7 -0.42853999269474485 2216 8 -0.3355070728158509 2216 9 -0.4676465219187804 2216 10-11 -0.46454450864418817 2216 12-13 -0.576134407054731 2216 14-15 -0.6230872894925881 2216 16-17 -0.6502333880125448 2216 18-19 -0.6461686065900878 2216 20-21 -0.6090429910016582 2216 22-23 -0.51036001770116 2216 24-25 -0.5521401312738163 2216 26-27 -0.5691487227946084 2216 28-29 -0.5650023804479503 2216 30-31 -0.6361996874283591 2216 32-33 -0.6175016065415377 2216 34-35 -0.7911711619716186 2216 36-37 -0.855766068035777 2216 38-39 -0.8393019713805288 2216 40-41 -0.7714080861983064 2216 42-43 -0.8424411838447128 2216 44-45 -0.6836144206190795 2216 46-47 -0.6662838807086615 2216 48-49 -0.7098177682197928 2216 50-51 -0.602925957782638 2216 52-53 -0.6850133084450718 2216 54-55 -0.671833957358686 2216 56-57 -0.8372590334686763 2216 58-59 -0.7379513081372764 2216 60-61 -0.7077888449557079 2216 62-63 -0.7119399084011278 2216 64-65 -0.7221619433577828 2216 66-67 -0.7673263040643477 2216 68-69 -0.7066809660938418 2216 70-71 -0.6850277612961619 2216 72-73 -0.5535438515911579 2216 74-75 -0.7107545975889096 2216 76-77 -0.5257614397586252 2216 78-79 -0.5247581157112577 2216 80-81 -0.49267363106097406 2216 82-83 -0.7724327298995917 2216 84-85 -0.5834014482702514 2216 86-87 -0.5837012030829953 2216 88-89 -0.7000748521445068 2216 90-91 -0.6483824840029655 2216 92-93 -0.8119730725791499 2216 94-95 -0.7407308685466205 2216 96-97 -0.6978226753284176 2216 98-99 -0.6627695201679096 2216 100 -0.6139131726521683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29595.0 3 6178.0 4 3378.0 5 2767.0 6 2273.0 7 2614.0 8 3163.0 9 3465.0 10 3990.0 11 4450.0 12 4829.0 13 5398.0 14 6066.0 15 6025.0 16 6309.0 17 6380.0 18 6239.0 19 6099.0 20 6039.0 21 6402.0 22 7321.0 23 10456.0 24 16015.0 25 21791.0 26 22866.0 27 19837.0 28 21076.0 29 26254.0 30 31940.0 31 38666.0 32 49509.0 33 66597.0 34 94801.0 35 145380.0 36 263442.0 37 557361.0 38 938108.0 39 419123.0 40 858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07611078334196 19.723382958434303 13.325011789241106 23.875494468982627 2 15.35193479610551 19.785204883397583 41.02072773223421 23.8421325882627 3 18.376748523902094 24.865519592731406 29.746411359946396 27.011320523420103 4 11.961929814539511 16.389623073954304 39.513387402173805 32.13505970933238 5 12.629749468042858 38.06872732998622 36.13166479244963 13.169858409521302 6 31.17019568115652 38.2444817816318 17.74511180312255 12.840210734089128 7 27.40592617623851 32.379336592945094 22.490728918632215 17.72400831218418 8 24.667645947991243 37.250447017767826 20.26056641297334 17.82134062126759 9 24.426220027147327 17.283288227145658 21.415765239713142 36.87472650599387 10-11 23.537119995958353 27.221098090931818 29.087049572012297 20.154732341097535 12-13 24.206416819019168 24.448299279195844 29.11749835974981 22.22778554203518 14-15 21.39344506487843 26.79205120517139 26.029318685431026 25.785185044519153 16-17 21.09363829191751 30.148157191629743 26.658071230886026 22.100133285566724 18-19 20.970595741308024 29.081951991944944 28.657702863603223 21.289749403143805 20-21 22.26546782064132 28.408822102077497 28.122261949180384 21.203448128100803 22-23 21.74758180944681 28.15033432140651 28.271267791977717 21.83081607716896 24-25 21.750418508256757 28.16692426153768 28.187203438791435 21.895453791414127 26-27 21.4718684007175 28.126839430128854 28.308881989137074 22.092410180016568 28-29 21.45754734067453 28.189975006130048 28.375896519248343 21.976581133947082 30-31 21.70170269196083 28.813361539107298 27.928331650299025 21.556604118632844 32-33 21.29926865935744 28.400127904497975 28.55997369458691 21.740629741557676 34-35 21.530687715287993 28.110847825409607 28.531062481102875 21.827401978199525 36-37 21.859977256451014 28.525672333748957 27.96422252739647 21.65012788240356 38-39 21.583318573058484 28.60412385533851 28.20727710286893 21.60528046873408 40-41 21.397088458511877 28.266810370803352 28.520092395164546 21.816008775520228 42-43 21.416754517029677 28.168521524108154 28.430391947014254 21.98433201184791 44-45 21.5130492179003 28.375359091870916 28.371057391131703 21.740534299097078 46-47 21.54342992129111 28.585272549841616 28.270374291761946 21.600923237105327 48-49 21.492131122758387 28.375138938864115 28.255802167080535 21.876927771296963 50-51 21.494165795889934 28.27183908045977 28.32410309996517 21.909892023685128 52-53 21.516626670282278 28.260976712925928 28.336894082642477 21.885502534149317 54-55 21.66260673430976 28.649637705893255 28.148548942792225 21.539206617004766 56-57 21.50651244139186 28.35184232840482 28.19954780106382 21.94209742913951 58-59 21.35749655845575 28.036086365563833 28.58995513149268 22.016461944487737 60-61 21.71145211855338 28.4524739718246 28.102244882784184 21.733829026837835 62-63 21.47100819430747 28.483512380452154 28.185627805021703 21.859851620218674 64-65 21.47075920432631 28.172488146847098 28.39147499440125 21.965277654425343 66-67 21.272172753520014 28.30221800242031 28.194385500150727 22.231223743908952 68-69 21.832547724701282 28.430319297795275 28.1195529910974 21.617579986406042 70-71 21.714548903963006 28.16889513446119 28.21274183816596 21.90381412340984 72-73 21.492858855546736 28.278900691590774 28.33986348582854 21.888376967033945 74-75 21.570342498636098 28.39569269485882 28.196936323954425 21.837028482550657 76-77 21.504746124065427 28.438262776157835 28.06103846037316 21.995952639403573 78-79 21.681204519874743 28.47618248325663 28.160067753546475 21.682545243322153 80-81 21.57229059041249 28.244397616572353 28.277182543793472 21.906129249221685 82-83 21.410138253662325 28.268321557252925 28.466888926073352 21.854651263011394 84-85 21.449613175098072 28.189782028398497 28.280490361238535 22.080114435264893 86-87 21.655702088460966 28.370160585354746 28.120099606549605 21.85403771963469 88-89 21.401331683295876 28.282356972185912 28.228854948754357 22.08745639576385 90-91 21.427916072773794 28.06504870139197 28.38975946750986 22.117275758324382 92-93 21.58442498139721 28.32519871122794 28.1779258959546 21.912450411420252 94-95 21.37707526672052 28.053497311902838 28.38164015710744 22.187787264269197 96-97 21.376005220381984 28.051439844516747 28.42042181953921 22.152133115562062 98-99 21.410642321184792 28.15596624656817 28.284630008115048 22.148761424131994 100 21.557775382670606 28.10988318338763 28.175282377911927 22.157059056029837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 285.0 1 278.0 2 269.0 3 280.5 4 307.0 5 311.0 6 310.5 7 285.5 8 248.0 9 228.0 10 221.0 11 225.0 12 219.0 13 236.0 14 273.5 15 312.0 16 393.0 17 554.0 18 767.0 19 1158.5 20 1757.5 21 2602.0 22 4153.5 23 6548.5 24 9289.5 25 12725.0 26 17580.0 27 23814.0 28 32042.5 29 41092.5 30 49760.5 31 58892.5 32 68062.5 33 76810.5 34 85223.0 35 92289.0 36 97378.0 37 102225.5 38 106889.0 39 108195.0 40 108080.5 41 107774.0 42 105373.5 43 103229.0 44 100466.5 45 97426.0 46 95588.5 47 92878.0 48 90142.0 49 88320.5 50 86040.5 51 82640.5 52 77394.5 53 72892.0 54 69950.5 55 66931.5 56 62238.5 57 56324.5 58 51290.0 59 46498.5 60 41194.0 61 35025.0 62 29281.0 63 23934.0 64 19059.5 65 15082.0 66 11605.5 67 8585.5 68 6511.5 69 5028.0 70 3540.5 71 2477.5 72 1769.5 73 1232.0 74 941.5 75 681.5 76 442.5 77 287.0 78 181.5 79 110.5 80 66.5 81 44.0 82 27.0 83 16.5 84 20.0 85 15.0 86 7.5 87 8.0 88 6.0 89 4.5 90 5.0 91 3.5 92 4.5 93 4.0 94 2.0 95 3.0 96 3.5 97 2.5 98 1.5 99 1.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06258135924763145 2 0.042010956958782625 3 0.0570123840086876 4 0.0749027169637947 5 0.08798980877531273 6 0.08781577829909575 7 0.08962569525175249 8 0.10191224687267234 9 0.09731784230054366 10-11 0.10241693525370162 12-13 0.10688951849247841 14-15 0.10843838973080965 16-17 0.07505934439239 18-19 0.09789214287205976 20-21 0.09164444877586962 22-23 0.05730823581825649 24-25 0.022815395432048063 26-27 0.1853076510758564 28-29 0.0680807222960885 30-31 0.078418132583378 32-33 0.07545961448768908 34-35 0.020866254098417716 36-37 0.05139119962687866 38-39 0.07582507848774478 40-41 0.08168990553625752 42-43 0.07568585410677119 44-45 0.07317981524924645 46-47 0.07975816725024885 48-49 0.07632976686877406 50-51 0.07170055620140199 52-53 0.07572066020201457 54-55 0.06654925410537893 56-57 0.06886385943906496 58-59 0.06587053524813266 60-61 0.06252915010476634 62-63 0.06896827772479518 64-65 0.06553987734332037 66-67 0.06754122781981581 68-69 0.06722797296262521 70-71 0.05890931619945285 72-73 0.0569601748658225 74-75 0.059361795437617035 76-77 0.05426270248445907 78-79 0.05137379657925696 80-81 0.04571780610220462 82-83 0.05187848496028624 84-85 0.04239382400646001 86-87 0.040392473529964563 88-89 0.04197615086353922 90-91 0.03896542362498521 92-93 0.041036386291967444 94-95 0.039678948577474886 96-97 0.04314215505419309 98-99 0.04293331848273269 100 0.05099092953157957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2873060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.04609001459295 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14550294887614 50.951588319825206 2 13.925618788094921 18.39465343974848 3 4.365438400198649 8.649604125980417 4 1.8275564763218206 4.828118381676131 5 0.8912958917748964 2.9433304348900853 6 0.5219402712812042 2.068326848356769 7 0.3202183854565255 1.4804420617132514 8 0.21539413992784517 1.1380752603432234 9 0.1498039027422032 0.8904565840543995 >10 0.6022463657886665 6.610843039685771 >50 0.027660449339151183 1.2451294194496094 >100 0.007271299520330911 0.7818961462926778 >500 5.268067759710846E-5 0.017535937984026216 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.480609524339902E-5 0.0 5 0.0 0.0 0.0 6.961219048679804E-5 0.0 6 0.0 0.0 0.0 6.961219048679804E-5 0.0 7 0.0 0.0 0.0 6.961219048679804E-5 0.0 8 0.0 0.0 0.0 6.961219048679804E-5 0.0 9 0.0 0.0 0.0 1.392243809735961E-4 0.0 10-11 0.0 0.0 0.0 2.2623961908209365E-4 0.0 12-13 0.0 0.0 0.0 2.6104571432549264E-4 0.0 14-15 0.0 0.0 0.0 2.784487619471922E-4 3.480609524339902E-5 16-17 0.0 0.0 0.0 2.784487619471922E-4 3.480609524339902E-5 18-19 0.0 0.0 0.0 2.958518095688917E-4 3.480609524339902E-5 20-21 0.0 0.0 0.0 3.828670476773893E-4 3.480609524339902E-5 22-23 0.0 0.0 0.0 4.350761905424878E-4 3.480609524339902E-5 24-25 0.0 0.0 0.0 4.872853334075863E-4 3.480609524339902E-5 26-27 0.0 0.0 0.0 5.917036191377834E-4 3.480609524339902E-5 28-29 0.0 0.0 0.0 9.571676191934733E-4 3.480609524339902E-5 30-31 0.0 0.0 0.0 0.002941115048067218 3.480609524339902E-5 32-33 0.0 0.0 0.0 0.008162029334577072 3.480609524339902E-5 34-35 0.0 0.0 0.0 0.015210263621365374 3.480609524339902E-5 36-37 0.0 0.0 0.0 0.02923712000445518 3.480609524339902E-5 38-39 0.0 0.0 0.0 0.053740611055808095 3.480609524339902E-5 40-41 0.0 0.0 0.0 0.08407412306043034 6.961219048679804E-5 42-43 0.0 0.0 0.0 0.11590429716051875 6.961219048679804E-5 44-45 0.0 0.0 0.0 0.1500142704990498 6.961219048679804E-5 46-47 0.0 1.740304762169951E-5 0.0 0.1935392926009203 6.961219048679804E-5 48-49 0.0 3.480609524339902E-5 0.0 0.24592246594223582 6.961219048679804E-5 50-51 0.0 3.480609524339902E-5 0.0 0.30380500233200836 6.961219048679804E-5 52-53 0.0 3.480609524339902E-5 0.0 0.3603997131977752 6.961219048679804E-5 54-55 0.0 3.480609524339902E-5 0.0 0.4233117303502189 6.961219048679804E-5 56-57 0.0 3.480609524339902E-5 0.0 0.49842328388547397 6.961219048679804E-5 58-59 0.0 3.480609524339902E-5 0.0 0.587091811518033 6.961219048679804E-5 60-61 0.0 3.480609524339902E-5 0.0 0.6838353532470607 6.961219048679804E-5 62-63 0.0 3.480609524339902E-5 0.0 0.7817623022143638 6.961219048679804E-5 64-65 0.0 3.480609524339902E-5 0.0 0.8904791407071206 6.961219048679804E-5 66-67 0.0 3.480609524339902E-5 0.0 1.011151872915985 6.961219048679804E-5 68-69 0.0 3.480609524339902E-5 0.0 1.1514030336992613 6.961219048679804E-5 70-71 0.0 3.480609524339902E-5 0.0 1.3028095480080473 6.961219048679804E-5 72-73 0.0 3.480609524339902E-5 0.0 1.4654584310804508 6.961219048679804E-5 74-75 0.0 3.480609524339902E-5 0.0 1.6331367949155258 6.961219048679804E-5 76-77 0.0 3.480609524339902E-5 0.0 1.810108386180588 6.961219048679804E-5 78-79 0.0 3.480609524339902E-5 0.0 2.0151684963070733 6.961219048679804E-5 80-81 0.0 3.480609524339902E-5 0.0 2.2442796182467473 6.961219048679804E-5 82-83 0.0 3.480609524339902E-5 0.0 2.4837977626641976 6.961219048679804E-5 84-85 0.0 3.480609524339902E-5 0.0 2.7221150967957506 6.961219048679804E-5 86-87 0.0 3.480609524339902E-5 0.0 2.9761125768344554 6.961219048679804E-5 88 0.0 3.480609524339902E-5 0.0 3.1898742107717903 6.961219048679804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3150 0.0 37.009502 1 ATCAACG 3035 0.0 35.78064 3 TCAACGC 3025 0.0 35.58811 4 GGTATCA 2005 0.0 35.402565 1 CAACGCA 3085 0.0 34.895958 5 AACGCAG 3485 0.0 30.884226 6 TATCAAC 3885 0.0 28.315203 2 GTACATG 7030 0.0 27.54952 1 TACATGG 7155 0.0 26.149794 2 CGCAGAG 4075 0.0 25.628931 8 TATACCG 150 1.1612847E-6 25.072266 5 ACGCAGA 4195 0.0 24.985733 7 ACATGGG 7535 0.0 23.520845 3 TCTACGC 220 6.3004336E-8 21.368406 3 CTGTCGC 700 0.0 20.16492 9 GAGTACA 5350 0.0 19.50614 1 CATGGGG 5330 0.0 19.139359 4 GCAGAGT 5405 0.0 18.977285 9 TCTGTCG 745 0.0 18.943933 8 AGTACAT 5415 0.0 17.970772 2 >>END_MODULE