FastQCFastQC Report
Wed 25 May 2016
SRR1033066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873060
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.16560740116809256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.14089507354527925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.055.17271
GTATCAA70800.043.986161
TCAACGC85400.035.991564
ATCAACG86000.035.6858063
CAACGCA87400.035.167345
AACGCAG91600.033.5548676
TATCAAC93850.032.7687642
ACGCAGA106100.028.9248457
CGCAGAG107100.028.5231298
GTACATG71300.027.9510841
CGTACAC1555.0909875E-827.2892513
TACATGG70600.026.8832362
TAGACCG1258.032921E-626.318515
GTGGTAT18550.024.8902911
GCAGAGT121150.024.5169779
ACATGGG73500.024.4901963
CCGATCG1201.8396856E-423.498679
TGGTATC18500.022.6568452
AGAGTAC111050.020.84303510-11
GAGTACT90100.020.83862312-13
Produced by FastQC (version 0.11.5)