##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2873060 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70922048269093 34.0 31.0 34.0 31.0 34.0 2 32.71130676003982 34.0 31.0 34.0 31.0 34.0 3 32.82348784919215 34.0 31.0 34.0 31.0 34.0 4 36.308170382797435 37.0 37.0 37.0 35.0 37.0 5 36.2861429973617 37.0 37.0 37.0 35.0 37.0 6 36.359604393921465 37.0 37.0 37.0 35.0 37.0 7 36.314527019971734 37.0 37.0 37.0 35.0 37.0 8 36.31845036302757 37.0 37.0 37.0 35.0 37.0 9 38.12046215533264 39.0 39.0 39.0 37.0 39.0 10-11 38.08996540274133 39.0 39.0 39.0 36.0 39.0 12-13 38.060027462009145 39.0 39.0 39.0 35.0 39.0 14-15 39.566571878067286 41.0 40.0 41.0 37.0 41.0 16-17 39.56499289955657 41.0 40.0 41.0 37.0 41.0 18-19 39.582434930004936 41.0 40.0 41.0 37.0 41.0 20-21 39.57889776057583 41.0 40.0 41.0 37.0 41.0 22-23 39.50681677375342 41.0 39.0 41.0 37.0 41.0 24-25 39.4576211426145 41.0 39.0 41.0 36.0 41.0 26-27 39.34995544819809 41.0 39.0 41.0 36.0 41.0 28-29 39.29906771873891 41.0 39.0 41.0 36.0 41.0 30-31 39.29456868286775 41.0 39.0 41.0 36.0 41.0 32-33 39.252500121821335 41.0 39.0 41.0 35.5 41.0 34-35 39.1730016080416 41.0 39.0 41.0 35.0 41.0 36-37 39.08147898059909 41.0 39.0 41.0 35.0 41.0 38-39 38.984060722713764 41.0 39.0 41.0 35.0 41.0 40-41 38.881743332892455 41.0 39.0 41.0 35.0 41.0 42-43 38.76374649328591 41.0 39.0 41.0 35.0 41.0 44-45 38.59653801173661 41.0 39.0 41.0 35.0 41.0 46-47 38.398177204792105 41.0 38.0 41.0 34.5 41.0 48-49 38.19366616081808 40.5 38.0 41.0 34.0 41.0 50-51 38.055714464717056 40.0 38.0 41.0 34.0 41.0 52-53 37.926332551356396 40.0 38.0 41.0 34.0 41.0 54-55 37.78337939339937 40.0 38.0 41.0 33.0 41.0 56-57 37.638057332600084 40.0 37.0 41.0 33.0 41.0 58-59 37.46618970714151 40.0 37.0 41.0 33.0 41.0 60-61 37.28071411665611 40.0 36.5 41.0 33.0 41.0 62-63 37.05457717555498 39.5 36.0 41.0 32.5 41.0 64-65 36.7943735947039 39.0 35.5 41.0 32.0 41.0 66-67 36.52589521276966 39.0 35.0 41.0 32.0 41.0 68-69 36.22511886281525 38.5 35.0 40.5 31.5 41.0 70-71 35.90069090099058 37.0 35.0 40.0 31.0 41.0 72-73 35.559606656317655 37.0 35.0 39.5 31.0 41.0 74-75 35.212016456321834 36.5 35.0 39.0 31.0 41.0 76-77 34.85499641497219 36.0 35.0 39.0 31.0 40.5 78-79 34.48828948925536 35.5 35.0 37.5 30.0 39.5 80-81 34.149843546601886 35.0 34.0 37.0 30.0 39.0 82-83 33.844998886204955 35.0 34.0 37.0 30.0 39.0 84-85 33.610031464710104 35.0 34.0 36.0 30.0 38.0 86-87 33.38859386855826 35.0 34.0 36.0 30.0 37.0 88-89 33.171182467473706 35.0 34.0 36.0 30.0 37.0 90-91 32.98170974501055 35.0 34.0 35.0 30.0 36.5 92-93 32.804140533090155 35.0 34.0 35.0 29.0 36.0 94-95 32.64261693107697 35.0 34.0 35.0 29.0 36.0 96-97 32.50067436809534 35.0 34.0 35.0 29.0 36.0 98-99 32.372434964811035 35.0 34.0 35.0 29.0 36.0 100 32.26825057604088 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12321669255524625 1101 2 0.13337607801857132 1101 3 0.2033558547786285 1101 4 0.16190288274818698 1101 5 0.1288538254554794 1101 6 0.11815343974502923 1101 7 0.14347855036029245 1101 8 0.16462996695982923 1101 9 0.23524492509130823 1101 10-11 0.26156213025719666 1101 12-13 0.21674281628188652 1101 14-15 0.30280777773926104 1101 16-17 0.3341220840164283 1101 18-19 0.3579297806920039 1101 20-21 0.335244287417666 1101 22-23 0.3453677398670578 1101 24-25 0.3489494722069111 1101 26-27 0.3413650872387066 1101 28-29 0.3822174540068346 1101 30-31 0.3319739044105887 1101 32-33 0.3138237713705081 1101 34-35 0.3384601054404044 1101 36-37 0.3414960891276806 1101 38-39 0.30067836996030195 1101 40-41 0.2702663711302904 1101 42-43 0.285094884087826 1101 44-45 0.30970755280414863 1101 46-47 0.31530390781143325 1101 48-49 0.27841228523635664 1101 50-51 0.27279985306668664 1101 52-53 0.2279658274267291 1101 54-55 0.25898160659154 1101 56-57 0.23875102863960507 1101 58-59 0.18513547861795132 1101 60-61 0.18926756624066599 1101 62-63 0.22173461560943508 1101 64-65 0.264019675470756 1101 66-67 0.21938210723772045 1101 68-69 0.1796966481357245 1101 70-71 0.16701904768974885 1101 72-73 0.14192703517481675 1101 74-75 0.18302540175743331 1101 76-77 0.10079536266589173 1101 78-79 0.13048275296452516 1101 80-81 0.07616743543102444 1101 82-83 0.06926976036831434 1101 84-85 0.10588646247576605 1101 86-87 0.0891582605359389 1101 88-89 0.1874321744648384 1101 90-91 0.20278871761382788 1101 92-93 0.21167807440363617 1101 94-95 0.20870909888716938 1101 96-97 0.23751466044532066 1101 98-99 0.20726679023452732 1101 100 0.18202803490049035 1102 1 0.133093676263897 1102 2 0.08570800846341342 1102 3 0.1398139262529554 1102 4 0.0881425739505346 1102 5 0.14295988274414384 1102 6 0.15521618589249186 1102 7 0.17075513563780476 1102 8 0.16415003853687438 1102 9 0.2228896402022258 1102 10-11 0.20290915999897408 1102 12-13 0.17841132102981305 1102 14-15 0.26951012480684966 1102 16-17 0.258211084992233 1102 18-19 0.27471078682525274 1102 20-21 0.2610042529266039 1102 22-23 0.2977220381389927 1102 24-25 0.33922406262563953 1102 26-27 0.34942419506649713 1102 28-29 0.35553608269009374 1102 30-31 0.3470398861216779 1102 32-33 0.29325719633759206 1102 34-35 0.28896632112842724 1102 36-37 0.2712304323244794 1102 38-39 0.31318266106912773 1102 40-41 0.33678180155216353 1102 42-43 0.3040645584501078 1102 44-45 0.2810115903658783 1102 46-47 0.25464553683797675 1102 48-49 0.33156655431523774 1102 50-51 0.2648827910773903 1102 52-53 0.23891470984628427 1102 54-55 0.1977066674779806 1102 56-57 0.2068281523667963 1102 58-59 0.14754198650683037 1102 60-61 0.13722932333822513 1102 62-63 0.10977512637978037 1102 64-65 0.17236240193994234 1102 66-67 0.1641591182472837 1102 68-69 0.21970924439848005 1102 70-71 0.15602367262656003 1102 72-73 0.11058491148817495 1102 74-75 0.15955377983993912 1102 76-77 0.07525373688301329 1102 78-79 0.0542106250340737 1102 80-81 0.05485515353423409 1102 82-83 0.08751136533761894 1102 84-85 0.12692870861106798 1102 86-87 0.12702827575417075 1102 88-89 0.08179271140080857 1102 90-91 0.050168708477478674 1102 92-93 0.10068601058977578 1102 94-95 0.1411522467635109 1102 96-97 0.0932633271606349 1102 98-99 0.1116349362862934 1102 100 0.11501186248847262 1103 1 0.13448254592641717 1103 2 0.042458920598832606 1103 3 0.07730290736459011 1103 4 0.11859140282063407 1103 5 0.11158337774356397 1103 6 0.14077847111395414 1103 7 0.13282443259419807 1103 8 0.11891461239613932 1103 9 0.17100383336624247 1103 10-11 0.2170323712171225 1103 12-13 0.19599110039347067 1103 14-15 0.27054521469355564 1103 16-17 0.27786434163366636 1103 18-19 0.30035733928116315 1103 20-21 0.2633093390757111 1103 22-23 0.24761861403657548 1103 24-25 0.22682790789446017 1103 26-27 0.20514561327807002 1103 28-29 0.2787451721531511 1103 30-31 0.2585429330157183 1103 32-33 0.27393914244240136 1103 34-35 0.2776403730730692 1103 36-37 0.24653042686564675 1103 38-39 0.3310824615995003 1103 40-41 0.35521680989582904 1103 42-43 0.3473406793208227 1103 44-45 0.39356971459390167 1103 46-47 0.34562991829015033 1103 48-49 0.3763364446657036 1103 50-51 0.4656793571281739 1103 52-53 0.42448715529903325 1103 54-55 0.39282153555299715 1103 56-57 0.32449838782559226 1103 58-59 0.35686044655008686 1103 60-61 0.4558895675546637 1103 62-63 0.3806061797554747 1103 64-65 0.47739102427787117 1103 66-67 0.4824512826194507 1103 68-69 0.43265737524240677 1103 70-71 0.4048459085408638 1103 72-73 0.3743969492988555 1103 74-75 0.41398967413486787 1103 76-77 0.3868545139709383 1103 78-79 0.3682200168488947 1103 80-81 0.3795794756391686 1103 82-83 0.3695542274094805 1103 84-85 0.3118872958740013 1103 86-87 0.33804472200824165 1103 88-89 0.3061821703045098 1103 90-91 0.309496042398834 1103 92-93 0.38718961250930306 1103 94-95 0.36477603168211914 1103 96-97 0.3582256716196426 1103 98-99 0.36761171193036546 1103 100 0.391404630388827 1104 1 0.07468746078176025 1104 2 0.10689020481489564 1104 3 0.06218036699956997 1104 4 0.09959713469726239 1104 5 0.11318797280623016 1104 6 0.12719000447495432 1104 7 0.14295497591072603 1104 8 0.1562279084088587 1104 9 0.13213456259128264 1104 10-11 0.16393715721302016 1104 12-13 0.20123636728231276 1104 14-15 0.24475026808859468 1104 16-17 0.2601254721939412 1104 18-19 0.24051563621654282 1104 20-21 0.23921053386484203 1104 22-23 0.25589586038049106 1104 24-25 0.26016319354873474 1104 26-27 0.24410574206617497 1104 28-29 0.2318426951522099 1104 30-31 0.18705607354359444 1104 32-33 0.15030632245057518 1104 34-35 0.13648149913925067 1104 36-37 0.19867372171635367 1104 38-39 0.1109547084749849 1104 40-41 0.157550603088346 1104 42-43 0.16321588321164882 1104 44-45 0.15918310411152703 1104 46-47 0.11765552448111549 1104 48-49 0.006158622643660294 1104 50-51 0.019411578414484154 1104 52-53 0.013213947318476471 1104 54-55 -0.005927345851581833 1104 56-57 0.024101240692111503 1104 58-59 0.011379672187018741 1104 60-61 0.07271620251864164 1104 62-63 -0.016986483372363637 1104 64-65 -0.06224093444004808 1104 66-67 -0.018198395976583015 1104 68-69 -0.021271041783855082 1104 70-71 -0.12053260550386824 1104 72-73 -0.10806820505745662 1104 74-75 -0.11628841458547612 1104 76-77 -0.12209756587347442 1104 78-79 -0.23197831893516252 1104 80-81 -0.16755355669884153 1104 82-83 -0.11984892492554877 1104 84-85 -0.09770172242080832 1104 86-87 -0.10551177099509346 1104 88-89 -0.08066183969012286 1104 90-91 -0.041091678165713574 1104 92-93 -0.06251368789551037 1104 94-95 -0.03791991704542852 1104 96-97 0.028798262948370734 1104 98-99 -0.019738869032167372 1104 100 0.031303287411027725 1105 1 -0.00789936195889851 1105 2 0.025211960372246267 1105 3 0.08052008371404185 1105 4 0.03968832988648785 1105 5 0.024362998052353646 1105 6 0.09718870088050124 1105 7 0.1566237926266396 1105 8 0.05593841405186595 1105 9 0.10667238898800235 1105 10-11 0.09858375910432216 1105 12-13 0.15666190036017014 1105 14-15 0.2078162869781366 1105 16-17 0.18945451953440084 1105 18-19 0.19107954954831285 1105 20-21 0.2159946509828714 1105 22-23 0.2053953870781413 1105 24-25 0.18175236922448335 1105 26-27 0.17868469575119406 1105 28-29 0.17662530226353113 1105 30-31 0.13334751189022143 1105 32-33 0.229808010834617 1105 34-35 0.18506422513744525 1105 36-37 0.2158867813583285 1105 38-39 0.2612865960997368 1105 40-41 0.29108780966058134 1105 42-43 0.2728501456234511 1105 44-45 0.2402292110365991 1105 46-47 0.2688510287376573 1105 48-49 0.27734053378365786 1105 50-51 0.22840307119705017 1105 52-53 0.2532702781150249 1105 54-55 0.2590014260531248 1105 56-57 0.26501669195383926 1105 58-59 0.26523767701513634 1105 60-61 0.27451982359605864 1105 62-63 0.2500072338665973 1105 64-65 0.2542377395657738 1105 66-67 0.22616267641264187 1105 68-69 0.3194339183518977 1105 70-71 0.23204376262086157 1105 72-73 0.3413130695193871 1105 74-75 0.3107904042145435 1105 76-77 0.2713945356253902 1105 78-79 0.2916164761848066 1105 80-81 0.3165024781147707 1105 82-83 0.3613295338637599 1105 84-85 0.33220239344652214 1105 86-87 0.23880419867170843 1105 88-89 0.23792653560919064 1105 90-91 0.2726220590750188 1105 92-93 0.3056895334286196 1105 94-95 0.3015537105570161 1105 96-97 0.37634973876657796 1105 98-99 0.31991554560984525 1105 100 0.30493458082474945 1106 1 -0.046700821253658376 1106 2 -0.0023208066525626236 1106 3 0.023069907546286572 1106 4 0.0065357714542599865 1106 5 0.022833973925727946 1106 6 0.013010600790195781 1106 7 0.048223403848759006 1106 8 -0.0026092552362797505 1106 9 0.012813284083748044 1106 10-11 0.04808363200044852 1106 12-13 0.04409124882389648 1106 14-15 0.0525877288694403 1106 16-17 0.0980146658905241 1106 18-19 0.12284132043424023 1106 20-21 0.0692805529404481 1106 22-23 0.08936820995754857 1106 24-25 0.04204103946902649 1106 26-27 0.0727939419807413 1106 28-29 0.10698565287469819 1106 30-31 0.06928537853602279 1106 32-33 0.042211238562103404 1106 34-35 0.013276109083605547 1106 36-37 0.03866214124897027 1106 38-39 0.020858718219010086 1106 40-41 0.013690368270580677 1106 42-43 0.03710611239510797 1106 44-45 0.04591498757908852 1106 46-47 -0.04619965948985083 1106 48-49 -0.028088368483231818 1106 50-51 -0.021397773466006242 1106 52-53 0.01579978754737965 1106 54-55 0.04939573917665285 1106 56-57 0.06111362510608842 1106 58-59 0.06700167666315338 1106 60-61 0.03820582279254836 1106 62-63 0.051351479616172924 1106 64-65 0.03198466107424025 1106 66-67 0.051924366414276335 1106 68-69 0.08932334242020801 1106 70-71 0.08177964431293105 1106 72-73 0.08664616834754213 1106 74-75 0.12547861306310892 1106 76-77 0.07715819662601575 1106 78-79 0.1204920445969151 1106 80-81 0.04258106119981875 1106 82-83 0.05484385838218486 1106 84-85 0.09556546656946807 1106 86-87 -0.019026578066331012 1106 88-89 0.006682135290631663 1106 90-91 -0.08397315461949972 1106 92-93 -0.06997070678882977 1106 94-95 -0.08167326586388413 1106 96-97 -0.073287015231422 1106 98-99 -0.10809005007182293 1106 100 -0.06306515074607688 1107 1 0.029164946962062288 1107 2 -0.015598760960273239 1107 3 0.014850547122108537 1107 4 0.022961672753993412 1107 5 0.05040390883659995 1107 6 0.02199005361767803 1107 7 0.018328109648663826 1107 8 0.034767741194755786 1107 9 0.029269738641012566 1107 10-11 0.05458089972731983 1107 12-13 0.04154993355530223 1107 14-15 0.10655103120225817 1107 16-17 -0.006569829036351393 1107 18-19 0.01994724323662922 1107 20-21 0.05247989350286275 1107 22-23 0.08671029021135013 1107 24-25 0.07807545525655257 1107 26-27 0.03852333583829903 1107 28-29 0.054412523759623355 1107 30-31 0.08895056611166297 1107 32-33 0.06578115381634575 1107 34-35 0.11232688560639303 1107 36-37 0.11937129517584566 1107 38-39 0.12143726444801217 1107 40-41 0.10693837611272272 1107 42-43 0.15042120066198095 1107 44-45 0.1639558421985683 1107 46-47 0.20542537854147014 1107 48-49 0.19048733907037985 1107 50-51 0.21846126918719477 1107 52-53 0.1939534819703681 1107 54-55 0.1701085082162379 1107 56-57 0.16721675581079154 1107 58-59 0.20637361920081077 1107 60-61 0.20452739433246592 1107 62-63 0.14232813051764737 1107 64-65 0.17255971824942407 1107 66-67 0.1793731138589152 1107 68-69 0.14273524391821724 1107 70-71 0.11705199135007405 1107 72-73 0.138093847923777 1107 74-75 0.1681003004917443 1107 76-77 0.1750013182544734 1107 78-79 0.22067612263207081 1107 80-81 0.1792738783212684 1107 82-83 0.13756413241286225 1107 84-85 0.17676528871960073 1107 86-87 0.14393178489440572 1107 88-89 0.11965462112075897 1107 90-91 0.09026219135448343 1107 92-93 0.1735430823449775 1107 94-95 0.17445394422630045 1107 96-97 0.09870143777382623 1107 98-99 0.12638884168674736 1107 100 0.1851984413940002 1108 1 -0.02958278038512674 1108 2 -0.029171518511617478 1108 3 0.01801222111340195 1108 4 -0.014205872888133797 1108 5 -0.0025575821266698995 1108 6 0.043519955165422175 1108 7 0.024426124221250234 1108 8 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-0.17750636406164944 2214 48-49 -0.21010731621802137 2214 50-51 -0.2504445654423293 2214 52-53 -0.28621841659604286 2214 54-55 -0.27559675614359236 2214 56-57 -0.28690326899623386 2214 58-59 -0.23260863470265747 2214 60-61 -0.2469445477011547 2214 62-63 -0.2566078682166051 2214 64-65 -0.29723736540385204 2214 66-67 -0.23376352619462182 2214 68-69 -0.26485146921553593 2214 70-71 -0.2533877893561538 2214 72-73 -0.23825986499322482 2214 74-75 -0.25712449399351556 2214 76-77 -0.2534916444599844 2214 78-79 -0.18917728830169267 2214 80-81 -0.16260921566922804 2214 82-83 -0.17865299781256283 2214 84-85 -0.1736382846151372 2214 86-87 -0.1214281397732222 2214 88-89 -0.11993130209292246 2214 90-91 -0.0718078945110534 2214 92-93 -0.023530606878026106 2214 94-95 -0.11934377842296584 2214 96-97 -0.12462484703269894 2214 98-99 -0.12483802431256663 2214 100 -0.1480299534770637 2215 1 -0.23518144874026348 2215 2 -0.20334081734639398 2215 3 -0.2042391126455172 2215 4 -0.17663854379537725 2215 5 -0.20587394066158282 2215 6 -0.1931382102393968 2215 7 -0.22511294311442498 2215 8 -0.17598268565735964 2215 9 -0.26642946595649875 2215 10-11 -0.23901527734343375 2215 12-13 -0.23300967998736155 2215 14-15 -0.4328859404380978 2215 16-17 -0.43990802084912417 2215 18-19 -0.384583635870527 2215 20-21 -0.3709626168040572 2215 22-23 -0.3711120351957291 2215 24-25 -0.4239052426987229 2215 26-27 -0.37262114839941063 2215 28-29 -0.4213740293574375 2215 30-31 -0.5593597241477823 2215 32-33 -0.408409909357772 2215 34-35 -0.38937011185331016 2215 36-37 -0.39566222833224884 2215 38-39 -0.37818013029649933 2215 40-41 -0.36677117704598317 2215 42-43 -0.34585425429991545 2215 44-45 -0.42654461588663395 2215 46-47 -0.4335045760664471 2215 48-49 -0.4566777381794367 2215 50-51 -0.4601373024198523 2215 52-53 -0.37584464820912444 2215 54-55 -0.4254178733270919 2215 56-57 -0.3695992396916452 2215 58-59 -0.3792961221892739 2215 60-61 -0.3877117053218839 2215 62-63 -0.43345634039394554 2215 64-65 -0.4411677418763489 2215 66-67 -0.40146330807920094 2215 68-69 -0.29548832248331536 2215 70-71 -0.341889519498217 2215 72-73 -0.31332569565678625 2215 74-75 -0.38403552177119593 2215 76-77 -0.3918140525863265 2215 78-79 -0.4068381404754149 2215 80-81 -0.46899997334241306 2215 82-83 -0.44111720686170486 2215 84-85 -0.39711396418507405 2215 86-87 -0.3812863261287873 2215 88-89 -0.3482820173667278 2215 90-91 -0.34959330358575613 2215 92-93 -0.2940239646712257 2215 94-95 -0.29415680828275725 2215 96-97 -0.3657709313595561 2215 98-99 -0.4592730914328129 2215 100 -0.46532939769316073 2216 1 -0.1607750143637432 2216 2 -0.15874696542452682 2216 3 -0.19018171773069525 2216 4 -0.16636417210525423 2216 5 -0.18163309897308721 2216 6 -0.17832273602711268 2216 7 -0.17042412357017156 2216 8 -0.197958533007089 2216 9 -0.24026254823923665 2216 10-11 -0.21689717997609392 2216 12-13 -0.2810740007336321 2216 14-15 -0.3787651138845689 2216 16-17 -0.3333463206682765 2216 18-19 -0.3144347031592787 2216 20-21 -0.31020481085680984 2216 22-23 -0.2579491249366086 2216 24-25 -0.29180315430301107 2216 26-27 -0.285469058493085 2216 28-29 -0.2887960957700315 2216 30-31 -0.2563922557017406 2216 32-33 -0.29777183800185725 2216 34-35 -0.27284105544390513 2216 36-37 -0.2801703202182111 2216 38-39 -0.26080622387723196 2216 40-41 -0.3501024063019358 2216 42-43 -0.3141787361214057 2216 44-45 -0.3730394567608357 2216 46-47 -0.2946452161951214 2216 48-49 -0.31605972929123993 2216 50-51 -0.3316694412127035 2216 52-53 -0.34285877538027876 2216 54-55 -0.2650334062377411 2216 56-57 -0.31971960370783137 2216 58-59 -0.2931451774765108 2216 60-61 -0.334342717767953 2216 62-63 -0.33953235837175555 2216 64-65 -0.47120790637563204 2216 66-67 -0.468228632596265 2216 68-69 -0.4136644758414221 2216 70-71 -0.391305647017532 2216 72-73 -0.3478455667793412 2216 74-75 -0.45993025819684163 2216 76-77 -0.43783657304802404 2216 78-79 -0.4292057418559736 2216 80-81 -0.4057131383207846 2216 82-83 -0.3934845276986181 2216 84-85 -0.4896181435661191 2216 86-87 -0.4867631316429808 2216 88-89 -0.5984453261417002 2216 90-91 -0.6064211605631655 2216 92-93 -0.5877653775178118 2216 94-95 -0.4459715660297192 2216 96-97 -0.5274360442460093 2216 98-99 -0.6536391994579418 2216 100 -0.6244733583077071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 2.0 4 6.0 5 17.0 6 59.0 7 163.0 8 326.0 9 662.0 10 1135.0 11 1936.0 12 3057.0 13 4705.0 14 5955.0 15 7978.0 16 9497.0 17 13431.0 18 15688.0 19 8583.0 20 5770.0 21 5337.0 22 5359.0 23 5601.0 24 6488.0 25 7938.0 26 10194.0 27 13330.0 28 17423.0 29 22177.0 30 28451.0 31 36586.0 32 47022.0 33 61897.0 34 85128.0 35 128614.0 36 233678.0 37 535902.0 38 1016244.0 39 524715.0 40 1981.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98343880464378 19.841885313748275 13.490101552627298 23.684574328980645 2 15.601815878363645 20.05895917413891 40.523144912634145 23.8160800348633 3 18.417743218290383 24.99250779241799 30.077532601109997 26.51221638818163 4 12.123115949570467 16.601676505978595 39.17512675778864 32.100080786662296 5 12.318777272817453 37.83038461177013 36.22199103183965 13.62884708357277 6 30.915143376847944 38.51909707357108 17.307168877203313 13.258590672377654 7 27.05519550583698 32.69374812917238 22.35745163693066 17.893604728059977 8 24.99926036867402 36.549830441693906 20.04895141161684 18.40195777801523 9 24.743131017103714 16.441424822314886 21.52259263642249 37.29285152415891 10-11 23.718048674540785 26.74706809019847 28.938316947681237 20.596566287579506 12-13 24.75454741634355 23.980251021558896 29.33492513208913 21.930276430008423 14-15 21.651085083795966 26.119820399923427 26.852595673587302 25.37649884269331 16-17 20.69112783158708 29.672987749113965 27.380012408383752 22.2558720109152 18-19 20.430501062025602 28.119461999845115 30.355328270764755 21.094708667364532 20-21 21.688694871911785 27.4213825751577 30.04736415089283 20.84255840203769 22-23 21.175706081659026 27.179155717960995 30.238737038411877 21.4064011619681 24-25 21.123788036577487 27.3293923058074 30.120980541756698 21.425839115858412 26-27 20.870337307785412 27.3021590058121 30.254239515136916 21.573264171265567 28-29 20.850078000622336 27.350738969751355 30.313335565568906 21.48584746405741 30-31 21.16313738293393 27.87398579283321 29.853915185114673 21.108961639118192 32-33 20.7913339784063 27.544743235435387 30.384555143296694 21.27936764286162 34-35 21.020671339965055 27.327796843783283 30.293728637759042 21.357803178492617 36-37 21.37616339373351 27.728832673177727 29.687197622047574 21.20780631104119 38-39 21.175210402845746 27.895466854155497 29.77297724377493 21.156345499223825 40-41 21.02478943606613 27.544200718502598 29.971840113941294 21.459169731489975 42-43 21.095121577690684 27.501461856000226 29.769583649488702 21.633832916820396 44-45 21.244317904951515 27.770617390517426 29.594630811747752 21.390433892783303 46-47 21.366783151065412 28.02811288312809 29.22411992788177 21.380984037924723 48-49 21.387893047830538 27.935163205780594 28.987716928988604 21.689226817400264 50-51 21.44847305660167 27.924373316255142 28.818280857343737 21.808872769799446 52-53 21.461803791079895 27.95750871892686 28.81553117581951 21.76515631417374 54-55 21.541248703472952 28.384649119753853 28.636888891982764 21.43721328479043 56-57 21.31810682686752 28.038867966558307 28.716803686661606 21.926221519912566 58-59 21.20803255066027 27.676797560788845 29.165454254349022 21.949715634201862 60-61 21.560200622332985 28.020020465984004 28.69654653922995 21.723232372453065 62-63 21.31370385581923 28.027434164270847 28.776478736956417 21.88238324295351 64-65 21.327748115249943 27.75485022937217 28.910168948786314 22.00723270659158 66-67 21.179042759121415 27.845420535147287 28.78528968857067 22.190247017160626 68-69 21.680737055155575 28.070381527676986 28.650878594388203 21.598002822779236 70-71 21.653829018537728 27.764735160421296 28.645538206650752 21.935897614390232 72-73 21.45811441696342 27.907281905345986 28.708955247257883 21.925648430432716 74-75 21.561830363008617 28.03427079539104 28.526620672708198 21.877278168892143 76-77 21.487003404036116 28.048996540274135 28.454974835193138 22.009025220496614 78-79 21.6469896208224 28.147428177622462 28.522202808155768 21.68337939339937 80-81 21.570764272239355 27.936207388637897 28.58372258149847 21.909305757624274 82-83 21.3891883180958 27.97902862419463 28.725341178165852 21.90644187954372 84-85 21.370159342304024 27.88594738710643 28.62086068512318 22.12303258546637 86-87 21.57286323544307 28.066700614365164 28.43477673703215 21.925659413159618 88-89 21.26341282758486 27.97614406702968 28.51932268614366 22.241120419241796 90-91 21.179847876651476 27.853992215447455 28.674424533958 22.291735373943066 92-93 21.36616630357944 28.078917609074967 28.407384216130122 22.147531871215474 94-95 21.120942525643077 27.769842917613758 28.65820306075798 22.451011495985185 96-97 21.029481837638446 27.816172808266597 28.63519589064888 22.519149463446077 98-99 21.082584987392355 27.88216807976587 28.510940518614568 22.52430641422721 100 21.174914551036178 27.861791957007508 28.367907387941777 22.595386104014533 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 15.5 2 62.5 3 126.5 4 99.5 5 71.5 6 193.0 7 450.0 8 796.0 9 967.5 10 849.0 11 766.5 12 844.5 13 1013.5 14 1227.0 15 1452.0 16 1690.5 17 1973.0 18 2309.5 19 2791.5 20 3470.5 21 4336.0 22 5790.0 23 8136.5 24 10976.5 25 14337.5 26 18712.5 27 24750.5 28 32580.5 29 41303.5 30 49924.0 31 58815.5 32 67735.0 33 76186.5 34 84390.5 35 91323.5 36 96568.5 37 101594.5 38 106051.0 39 107023.0 40 106909.5 41 106878.5 42 104937.0 43 103014.5 44 100169.5 45 97370.0 46 95542.5 47 92249.5 48 89785.0 49 88507.5 50 85597.0 51 81676.5 52 76575.5 53 71932.0 54 69153.5 55 66406.0 56 61639.5 57 55532.5 58 50251.5 59 45584.5 60 40478.0 61 34468.5 62 28778.0 63 23528.5 64 18837.5 65 14690.5 66 11141.0 67 8311.0 68 6237.0 69 4696.5 70 3294.5 71 2287.5 72 1642.0 73 1164.5 74 803.5 75 504.5 76 300.0 77 199.0 78 132.0 79 72.0 80 39.0 81 17.0 82 5.0 83 1.0 84 0.0 85 2.0 86 2.0 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046883810292858485 2 0.24395592156098378 3 0.0026104571432549266 4 0.0021231718098473407 5 2.436426667037932E-4 6 6.961219048679804E-5 7 0.0 8 2.436426667037932E-4 9 0.0 10-11 6.265097143811824E-4 12-13 0.0 14-15 3.4806095243399026E-4 16-17 8.701523810849757E-5 18-19 4.350761905424878E-4 20-21 3.480609524339902E-5 22-23 1.218213333518966E-4 24-25 0.0012530194287623648 26-27 5.2209142865098537E-5 28-29 2.0883657146039415E-4 30-31 1.0441828573019707E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.2209142865098537E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.740304762169951E-5 68-69 1.7403047621699513E-4 70-71 0.0 72-73 2.436426667037932E-4 74-75 5.2209142865098537E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.480609524339902E-5 84-85 0.0 86-87 4.5247923816418725E-4 88-89 0.0022275900955775374 90-91 0.002175380952712439 92-93 4.5247923816418725E-4 94-95 4.6988228578588684E-4 96-97 1.0441828573019707E-4 98-99 5.2209142865098537E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2873060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.89311155103737 #Duplication Level Percentage of deduplicated Percentage of total 1 75.91336578254085 48.50341148158586 2 14.486136035838332 18.511286113626415 3 4.686109569172343 8.982303643305364 4 1.9359187585574977 4.947674927770399 5 0.9620181793124526 3.0733167422468206 6 0.5622836018544052 2.1555629337961553 7 0.34186947673979395 1.5290173229261301 8 0.22811061289761395 1.16597574686742 9 0.16351104087850696 0.9402506257209009 >10 0.678463056602226 7.197781674182677 >50 0.031738622398895515 1.3798278131518298 >100 0.009985151043665447 1.0849625064941084 >500 2.722845352869964E-4 0.1031648396812136 >1k 2.178276280977365E-4 0.4254636286447363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4758 0.16560740116809256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4048 0.14089507354527925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0441828573019707E-4 0.0 8 0.0 0.0 0.0 1.0441828573019707E-4 0.0 9 0.0 0.0 0.0 1.392243809735961E-4 0.0 10-11 0.0 0.0 0.0 2.0883657146039415E-4 0.0 12-13 0.0 0.0 0.0 3.132548571905912E-4 0.0 14-15 0.0 0.0 0.0 3.828670476773893E-4 0.0 16-17 0.0 0.0 0.0 3.828670476773893E-4 0.0 18-19 0.0 0.0 0.0 4.176731429207883E-4 0.0 20-21 0.0 0.0 0.0 5.046883810292859E-4 0.0 22-23 0.0 0.0 0.0 5.568975238943845E-4 1.740304762169951E-5 24-25 0.0 0.0 0.0 5.917036191377834E-4 3.480609524339902E-5 26-27 0.0 0.0 0.0 7.309280001113795E-4 3.480609524339902E-5 28-29 0.0 0.0 0.0 0.0010789889525453697 3.480609524339902E-5 30-31 0.0 0.0 0.0 0.0030629363814191142 3.480609524339902E-5 32-33 0.0 0.0 0.0 0.008231641525063869 3.480609524339902E-5 34-35 0.0 0.0 0.0 0.01533208495471727 3.480609524339902E-5 36-37 0.0 0.0 0.0 0.029428553528293877 3.480609524339902E-5 38-39 0.0 0.0 0.0 0.05405386591299868 3.480609524339902E-5 40-41 0.0 0.0 0.0 0.08449179620335114 3.480609524339902E-5 42-43 0.0 0.0 0.0 0.11637417944630464 3.480609524339902E-5 44-45 0.0 0.0 0.0 0.15072779545153947 3.480609524339902E-5 46-47 0.0 0.0 0.0 0.19437463888676185 3.480609524339902E-5 48-49 0.0 0.0 0.0 0.24687963356142928 3.480609524339902E-5 50-51 0.0 0.0 0.0 0.30491879737979716 3.480609524339902E-5 52-53 0.0 0.0 0.0 0.3617571509122678 3.480609524339902E-5 54-55 0.0 0.0 0.0 0.4249998259695238 3.480609524339902E-5 56-57 0.0 0.0 0.0 0.5004768435048346 3.480609524339902E-5 58-59 0.0 0.0 0.0 0.5896326564708012 3.480609524339902E-5 60-61 0.0 0.0 0.0 0.6874203810571307 3.480609524339902E-5 62-63 0.0 0.0 0.0 0.7859912427864368 3.480609524339902E-5 64-65 0.0 0.0 0.0 0.895317187945953 3.480609524339902E-5 66-67 0.0 0.0 0.0 1.0165642207263337 3.480609524339902E-5 68-69 0.0 0.0 0.0 1.157511503414478 3.480609524339902E-5 70-71 0.0 0.0 0.0 1.30949231829478 3.480609524339902E-5 72-73 0.0 0.0 0.0 1.4734464299388108 3.480609524339902E-5 74-75 0.0 0.0 0.0 1.6424996345360001 3.480609524339902E-5 76-77 0.0 0.0 0.0 1.8206372299917162 3.480609524339902E-5 78-79 0.0 0.0 0.0 2.027750899737562 3.480609524339902E-5 80-81 0.0 0.0 0.0 2.2589155812965966 3.480609524339902E-5 82-83 0.0 0.0 0.0 2.4997911634285397 3.480609524339902E-5 84-85 0.0 0.0 0.0 2.7391004712745297 3.480609524339902E-5 86-87 0.0 0.0 0.0 2.994368373789618 3.480609524339902E-5 88 0.0 0.0 0.0 3.2098877155367447 3.480609524339902E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3800 0.0 55.1727 1 GTATCAA 7080 0.0 43.98616 1 TCAACGC 8540 0.0 35.99156 4 ATCAACG 8600 0.0 35.685806 3 CAACGCA 8740 0.0 35.16734 5 AACGCAG 9160 0.0 33.554867 6 TATCAAC 9385 0.0 32.768764 2 ACGCAGA 10610 0.0 28.924845 7 CGCAGAG 10710 0.0 28.523129 8 GTACATG 7130 0.0 27.951084 1 CGTACAC 155 5.0909875E-8 27.289251 3 TACATGG 7060 0.0 26.883236 2 TAGACCG 125 8.032921E-6 26.31851 5 GTGGTAT 1855 0.0 24.890291 1 GCAGAGT 12115 0.0 24.516977 9 ACATGGG 7350 0.0 24.490196 3 CCGATCG 120 1.8396856E-4 23.49867 9 TGGTATC 1850 0.0 22.656845 2 AGAGTAC 11105 0.0 20.843035 10-11 GAGTACT 9010 0.0 20.838623 12-13 >>END_MODULE