FastQCFastQC Report
Wed 25 May 2016
SRR1033065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5047565
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65650.038.0947071
TCAACGC62850.037.847134
GGTATCA35300.036.888651
ATCAACG65550.036.503363
CAACGCA65250.036.311685
AACGCAG73500.032.2943956
TATCAAC83500.029.2752292
GTACATG142000.027.3450931
ACGCAGA85550.027.0873957
CGCAGAG86300.026.5999728
TACATGG141000.026.1718222
ACATGGG143650.024.8056893
CATGGGG92950.020.938174
GCAGAGT107250.020.4410829
GAGTACA105400.018.5987261
TATACTG27350.018.3917395
GTATAGG19700.018.3743611
AGAGTAC90400.017.02624510-11
ATACCGC4851.8189894E-1216.4751856
AGTACAT109650.016.4200152
Produced by FastQC (version 0.11.5)