FastQCFastQC Report
Wed 25 May 2016
SRR1033065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5047565
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.15383655287252368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.11762107075391798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.054.5444451
GTATCAA125950.044.7912031
TCAACGC151850.036.4905854
ATCAACG153000.036.3091853
CAACGCA155000.035.718335
AACGCAG163200.034.0385136
TATCAAC164600.033.890372
ACGCAGA185650.029.7198337
CGCAGAG186650.029.409538
GTACATG137550.026.5544821
GCAGAGT210150.025.7629939
TACATGG139450.025.1029452
ACATGGG142250.023.6895793
GAGTACT142700.021.62119512-13
AGAGTAC194350.021.3404410-11
TAGACCG1805.673288E-620.887915
CATGGGG91700.020.8083924
CAGAGTA206650.020.79799710-11
AGTACTT152750.019.49100912-13
ATACCGC4650.019.2032136
Produced by FastQC (version 0.11.5)