##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033065_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5047565
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74156568563258	34.0	31.0	34.0	31.0	34.0
2	32.73168844779612	34.0	31.0	34.0	31.0	34.0
3	32.843562787205315	34.0	31.0	34.0	31.0	34.0
4	36.32716765410648	37.0	37.0	37.0	35.0	37.0
5	36.316637626261375	37.0	37.0	37.0	35.0	37.0
6	36.38019361811091	37.0	37.0	37.0	35.0	37.0
7	36.328370808498754	37.0	37.0	37.0	35.0	37.0
8	36.333153510653155	37.0	37.0	37.0	35.0	37.0
9	38.146309160951866	39.0	39.0	39.0	37.0	39.0
10-11	38.10688757846605	39.0	39.0	39.0	36.0	39.0
12-13	38.085318663553615	39.0	39.0	39.0	36.0	39.0
14-15	39.57996112977247	41.0	40.0	41.0	37.0	41.0
16-17	39.57922433886438	41.0	40.0	41.0	37.0	41.0
18-19	39.60325661977607	41.0	40.0	41.0	37.0	41.0
20-21	39.59968103432051	41.0	40.0	41.0	37.0	41.0
22-23	39.5288812526436	41.0	39.5	41.0	37.0	41.0
24-25	39.47894687834629	41.0	39.0	41.0	36.5	41.0
26-27	39.374386560648546	41.0	39.0	41.0	36.0	41.0
28-29	39.326177275577436	41.0	39.0	41.0	36.0	41.0
30-31	39.321977032489926	41.0	39.0	41.0	36.0	41.0
32-33	39.281110000564624	41.0	39.0	41.0	35.5	41.0
34-35	39.203216996710296	41.0	39.0	41.0	35.0	41.0
36-37	39.11540168378218	41.0	39.0	41.0	35.0	41.0
38-39	39.02557272665136	41.0	39.0	41.0	35.0	41.0
40-41	38.93078207016651	41.0	39.0	41.0	35.0	41.0
42-43	38.81896904745159	41.0	39.0	41.0	35.0	41.0
44-45	38.661496285832875	41.0	39.0	41.0	35.0	41.0
46-47	38.469109461690934	41.0	38.5	41.0	35.0	41.0
48-49	38.276526503373404	41.0	38.0	41.0	34.0	41.0
50-51	38.150537536416074	40.0	38.0	41.0	34.0	41.0
52-53	38.029690751877396	40.0	38.0	41.0	34.0	41.0
54-55	37.89066490476101	40.0	38.0	41.0	34.0	41.0
56-57	37.75007246068154	40.0	37.0	41.0	33.5	41.0
58-59	37.58206679854544	40.0	37.0	41.0	33.0	41.0
60-61	37.399466277304	40.0	36.5	41.0	33.0	41.0
62-63	37.179243060762964	39.5	36.0	41.0	33.0	41.0
64-65	36.92394758660859	39.0	35.5	41.0	32.5	41.0
66-67	36.655441485151755	39.0	35.0	41.0	32.0	41.0
68-69	36.35512232135693	38.5	35.0	41.0	32.0	41.0
70-71	36.03384146613268	37.5	35.0	40.0	31.5	41.0
72-73	35.68825661482319	37.0	35.0	39.5	31.0	41.0
74-75	35.34132339058536	36.5	35.0	39.0	31.0	41.0
76-77	34.97766536934145	36.0	35.0	39.0	31.0	40.5
78-79	34.60804952486991	35.5	35.0	38.0	31.0	39.5
80-81	34.26645115020807	35.0	34.5	37.0	30.5	39.0
82-83	33.953333141821844	35.0	34.0	37.0	30.0	39.0
84-85	33.71668378713301	35.0	34.0	36.0	30.0	38.0
86-87	33.497515732833556	35.0	34.0	36.0	30.0	37.0
88-89	33.27520774868674	35.0	34.0	36.0	30.0	37.0
90-91	33.087729330875376	35.0	34.0	35.0	30.0	36.5
92-93	32.909345595351425	35.0	34.0	35.0	30.0	36.0
94-95	32.7545073713761	35.0	34.0	35.0	30.0	36.0
96-97	32.61272385793942	35.0	34.0	35.0	29.0	36.0
98-99	32.49186597894232	35.0	34.0	35.0	29.0	36.0
100	32.388346063894176	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14112944314738485
1101	2	0.11462482834066634
1101	3	0.1393111788249186
1101	4	0.0873149173527139
1101	5	0.118181006817764
1101	6	0.1205289784843373
1101	7	0.15753618031988026
1101	8	0.14632255840119512
1101	9	0.1952651784636359
1101	10-11	0.20135599050980346
1101	12-13	0.19292468434032628
1101	14-15	0.2723365306692358
1101	16-17	0.32040303137893034
1101	18-19	0.29359875300148275
1101	20-21	0.22954640603409615
1101	22-23	0.26117689332349414
1101	24-25	0.3024428180215324
1101	26-27	0.2882158561881738
1101	28-29	0.3045163018359034
1101	30-31	0.3152194157450907
1101	32-33	0.2746357065336653
1101	34-35	0.2797196235243078
1101	36-37	0.2741796315365477
1101	38-39	0.2658719849102482
1101	40-41	0.29472430458743304
1101	42-43	0.2921383333368226
1101	44-45	0.2466292170934139
1101	46-47	0.2655044878841508
1101	48-49	0.23411371956007088
1101	50-51	0.21176533461865432
1101	52-53	0.2338901193040357
1101	54-55	0.29889846525755104
1101	56-57	0.3186464551391879
1101	58-59	0.27664797188088386
1101	60-61	0.2614199414438545
1101	62-63	0.2930801391443296
1101	64-65	0.2751782631135171
1101	66-67	0.33100083074385367
1101	68-69	0.2651783085669521
1101	70-71	0.22808961599088207
1101	72-73	0.2843048782347779
1101	74-75	0.2610351638314796
1101	76-77	0.24913910795618932
1101	78-79	0.2034701403584407
1101	80-81	0.2573385254237266
1101	82-83	0.2727733294653376
1101	84-85	0.2122066141442076
1101	86-87	0.24914649361014085
1101	88-89	0.2964111890149539
1101	90-91	0.31987503256736716
1101	92-93	0.2996262134479295
1101	94-95	0.30981842834159323
1101	96-97	0.31756360855061416
1101	98-99	0.3530985161151037
1101	100	0.333643952482781
1102	1	0.1429899654961133
1102	2	0.07615471656880857
1102	3	0.10302849265722358
1102	4	0.07779419662595899
1102	5	0.10874679201926796
1102	6	0.09345058644105819
1102	7	0.08373743664481736
1102	8	0.09576607728224218
1102	9	0.1326328499062015
1102	10-11	0.1712174993383755
1102	12-13	0.18671553635764582
1102	14-15	0.24125558196055863
1102	16-17	0.25237229618240065
1102	18-19	0.24529385850149765
1102	20-21	0.21538302324002245
1102	22-23	0.26151568746706744
1102	24-25	0.2744010830180912
1102	26-27	0.2547042447642056
1102	28-29	0.24335707442639887
1102	30-31	0.2248170097624751
1102	32-33	0.24380180771374427
1102	34-35	0.24458346335161707
1102	36-37	0.24153380348349884
1102	38-39	0.303783021290414
1102	40-41	0.33309951553190587
1102	42-43	0.28282142084857753
1102	44-45	0.3076018673040082
1102	46-47	0.33630075402308535
1102	48-49	0.35042635945195855
1102	50-51	0.37082273045350433
1102	52-53	0.3323765076765497
1102	54-55	0.30388460541485074
1102	56-57	0.33719813003130383
1102	58-59	0.3450485465083446
1102	60-61	0.3828572712703675
1102	62-63	0.3439810939747261
1102	64-65	0.397292004583619
1102	66-67	0.41553441026118065
1102	68-69	0.42174587009291287
1102	70-71	0.3115304783275974
1102	72-73	0.3226196617449304
1102	74-75	0.31960293565984443
1102	76-77	0.2855886738573119
1102	78-79	0.28778478497940085
1102	80-81	0.3090275146400785
1102	82-83	0.3062778810280449
1102	84-85	0.3099250842098158
1102	86-87	0.28534696181019115
1102	88-89	0.30243657695488935
1102	90-91	0.2532480670606887
1102	92-93	0.29842369505964683
1102	94-95	0.33734523471401445
1102	96-97	0.29810902944653606
1102	98-99	0.30939775992023755
1102	100	0.2853067794824895
1103	1	0.13121090922975753
1103	2	0.08037014668492759
1103	3	0.1014454629522561
1103	4	0.09312424021931776
1103	5	0.11186455531165507
1103	6	0.09504405264883076
1103	7	0.1071540013790937
1103	8	0.12322510565736877
1103	9	0.16356271414017698
1103	10-11	0.1808568214434132
1103	12-13	0.20353334927582267
1103	14-15	0.2667834643113878
1103	16-17	0.29340982604296784
1103	18-19	0.27380756316895116
1103	20-21	0.2625078398616054
1103	22-23	0.23595620085304603
1103	24-25	0.24399961586412644
1103	26-27	0.24254361205188246
1103	28-29	0.26805076798180494
1103	30-31	0.25245815292808516
1103	32-33	0.1954862472570582
1103	34-35	0.25030004684321483
1103	36-37	0.22201932269394575
1103	38-39	0.22428524283540696
1103	40-41	0.18334435741594035
1103	42-43	0.13797764164942805
1103	44-45	0.20356903886960964
1103	46-47	0.18831875336602621
1103	48-49	0.14668444962477878
1103	50-51	0.20178173392731225
1103	52-53	0.14325328897525225
1103	54-55	0.1450138171141333
1103	56-57	0.10791737734199103
1103	58-59	0.11209633911922623
1103	60-61	0.13788145939396657
1103	62-63	0.08705450387691371
1103	64-65	0.11845552705003115
1103	66-67	0.08445883672786891
1103	68-69	0.09506886764952327
1103	70-71	0.07974896053127623
1103	72-73	0.1515049779632136
1103	74-75	0.11959965187318744
1103	76-77	0.17917670487415904
1103	78-79	0.18308919494985787
1103	80-81	0.16126092514749502
1103	82-83	0.18160233781735258
1103	84-85	0.20300957580028012
1103	86-87	0.1850606959618517
1103	88-89	0.1605614042094814
1103	90-91	0.21331005988731988
1103	92-93	0.273499372259316
1103	94-95	0.23785762333409366
1103	96-97	0.2470989626535669
1103	98-99	0.19466690477306514
1103	100	0.16476353462136473
1104	1	0.11121092972694413
1104	2	0.1401523130861122
1104	3	0.15448202747887763
1104	4	0.066568090456947
1104	5	0.09004143507951312
1104	6	0.1271981293847162
1104	7	0.12464807481744344
1104	8	0.12425032035019257
1104	9	0.1389363532030714
1104	10-11	0.16325702019079102
1104	12-13	0.1816486642916857
1104	14-15	0.22819456900306534
1104	16-17	0.25433131997217373
1104	18-19	0.2715716447386143
1104	20-21	0.2578100738516298
1104	22-23	0.250531151300585
1104	24-25	0.25020680067461853
1104	26-27	0.25271177282023416
1104	28-29	0.2753597600184179
1104	30-31	0.2284364588773542
1104	32-33	0.23108278397779003
1104	34-35	0.21682054489379254
1104	36-37	0.24521146648360315
1104	38-39	0.2368433541781485
1104	40-41	0.22456197456031646
1104	42-43	0.2520036670894825
1104	44-45	0.2506108154114628
1104	46-47	0.21086142928450613
1104	48-49	0.20812072820316274
1104	50-51	0.2168904268263745
1104	52-53	0.2583863818877816
1104	54-55	0.2643638811542388
1104	56-57	0.25899293876243945
1104	58-59	0.2518753489542931
1104	60-61	0.2789515093730017
1104	62-63	0.2593274253690012
1104	64-65	0.22378116777679935
1104	66-67	0.23795055583472902
1104	68-69	0.2504639383992142
1104	70-71	0.22390049001116807
1104	72-73	0.22685621862525807
1104	74-75	0.22726509828122232
1104	76-77	0.22380810686325248
1104	78-79	0.19247324436446434
1104	80-81	0.18805582525604336
1104	82-83	0.16413650029379312
1104	84-85	0.2356914340723435
1104	86-87	0.2695639077344083
1104	88-89	0.2639992439620542
1104	90-91	0.2563214419518971
1104	92-93	0.26512284303093026
1104	94-95	0.2607393393849904
1104	96-97	0.3120351769900154
1104	98-99	0.2875634199989534
1104	100	0.2908952483872511
1105	1	0.06246075337294599
1105	2	0.0751262929670915
1105	3	0.0996324404516713
1105	4	0.04484701487124454
1105	5	0.0325344287893472
1105	6	0.07013260033232882
1105	7	0.0822336029316304
1105	8	0.05701971377820314
1105	9	0.07553658807546526
1105	10-11	0.07785588284016853
1105	12-13	0.11272788847742987
1105	14-15	0.15311885768316813
1105	16-17	0.14298246516401747
1105	18-19	0.15330520048976126
1105	20-21	0.13160763133069509
1105	22-23	0.15669974481859583
1105	24-25	0.15933299213355667
1105	26-27	0.17275641549033338
1105	28-29	0.19384974653779352
1105	30-31	0.19018601658760304
1105	32-33	0.1925411789428395
1105	34-35	0.16899855930777363
1105	36-37	0.16731990608975167
1105	38-39	0.1555459444194156
1105	40-41	0.16053789769296145
1105	42-43	0.16462150121927266
1105	44-45	0.1167625486457311
1105	46-47	0.1611810553588242
1105	48-49	0.16181033569702663
1105	50-51	0.14712614736072993
1105	52-53	0.160695852713026
1105	54-55	0.17682277643013578
1105	56-57	0.14710249168455647
1105	58-59	0.19321306003039496
1105	60-61	0.18899263690613566
1105	62-63	0.22766182488614817
1105	64-65	0.23139202469059228
1105	66-67	0.1820748949648987
1105	68-69	0.19070751072440117
1105	70-71	0.2392951707400499
1105	72-73	0.24364851577254854
1105	74-75	0.22953527295302933
1105	76-77	0.21203044019605244
1105	78-79	0.2622346849370558
1105	80-81	0.30147814881521384
1105	82-83	0.2458471060419143
1105	84-85	0.24817489849922936
1105	86-87	0.19326666967975825
1105	88-89	0.19309644112097857
1105	90-91	0.19782975092197574
1105	92-93	0.22565687483898245
1105	94-95	0.18880291516148873
1105	96-97	0.16841008559234183
1105	98-99	0.17651605563313666
1105	100	0.17486918022240872
1106	1	0.030707133418168553
1106	2	0.04888860825681007
1106	3	0.05437346717162228
1106	4	0.0330861366186781
1106	5	0.04461992077985144
1106	6	0.0613846357583796
1106	7	0.07808359512259955
1106	8	0.0900455014197945
1106	9	0.09162986844433618
1106	10-11	0.11403374500988406
1106	12-13	0.06110505492188878
1106	14-15	0.12276333996788935
1106	16-17	0.14659707431426483
1106	18-19	0.12575223403896274
1106	20-21	0.13474738886989712
1106	22-23	0.1467601276397943
1106	24-25	0.14221125696685988
1106	26-27	0.16822507701077427
1106	28-29	0.16160573407318424
1106	30-31	0.16674321372661893
1106	32-33	0.2003703475786054
1106	34-35	0.1896160506518072
1106	36-37	0.15861508312429606
1106	38-39	0.1449067939121278
1106	40-41	0.14556882500002644
1106	42-43	0.1321654914232795
1106	44-45	0.14430276641598283
1106	46-47	0.2125619303466948
1106	48-49	0.21589506989419505
1106	50-51	0.20224831003073263
1106	52-53	0.19375164751556184
1106	54-55	0.2195325095848233
1106	56-57	0.17544414956338272
1106	58-59	0.19730512012642976
1106	60-61	0.19077534883559366
1106	62-63	0.21170071563630444
1106	64-65	0.24099021971256462
1106	66-67	0.21432756881694814
1106	68-69	0.18948799104692426
1106	70-71	0.17610583038580785
1106	72-73	0.20111124801759672
1106	74-75	0.22217662064213783
1106	76-77	0.22820853419336373
1106	78-79	0.24792823134553998
1106	80-81	0.22211973406330543
1106	82-83	0.2791630272003971
1106	84-85	0.2567087164685731
1106	86-87	0.23112155280834656
1106	88-89	0.27425681941217306
1106	90-91	0.24537733280037344
1106	92-93	0.2752980417319648
1106	94-95	0.2802355795515936
1106	96-97	0.2576760253833754
1106	98-99	0.29128145070280453
1106	100	0.3064590402525553
1107	1	-0.04360310702980996
1107	2	-0.008030976201673923
1107	3	0.005997891164234659
1107	4	-0.0014006541795978933
1107	5	0.007263079400836148
1107	6	0.054415042348139764
1107	7	0.046508838600239244
1107	8	0.0040930711170972245
1107	9	-0.005772533803053648
1107	10-11	0.01733835948387963
1107	12-13	0.006182396071004348
1107	14-15	0.06144277363095796
1107	16-17	0.05561825684443278
1107	18-19	0.06971695432280711
1107	20-21	0.03856666979808665
1107	22-23	0.08667370370029914
1107	24-25	0.08778238743211375
1107	26-27	0.09764463521840838
1107	28-29	0.10884234131412995
1107	30-31	0.06701339883518642
1107	32-33	0.030164979542284698
1107	34-35	-0.020269247739939544
1107	36-37	0.011759788074122923
1107	38-39	0.07672219342210695
1107	40-41	0.05019503466736808
1107	42-43	0.06044766915055533
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2216	32-33	-0.3310966166080078
2216	34-35	-0.33047463865910487
2216	36-37	-0.3243929137840311
2216	38-39	-0.34936483669724794
2216	40-41	-0.33634273284559413
2216	42-43	-0.3273502793778036
2216	44-45	-0.3298314362933539
2216	46-47	-0.26904500772491247
2216	48-49	-0.3654436206042888
2216	50-51	-0.3727964900437968
2216	52-53	-0.39464656719888325
2216	54-55	-0.40351533710209964
2216	56-57	-0.4149724700513957
2216	58-59	-0.3902737007065795
2216	60-61	-0.43571861011710666
2216	62-63	-0.3996659530529527
2216	64-65	-0.4801376267421489
2216	66-67	-0.45140252001194625
2216	68-69	-0.4239565648784307
2216	70-71	-0.4032534673867616
2216	72-73	-0.37555686625759677
2216	74-75	-0.45873148464332303
2216	76-77	-0.4188177291203701
2216	78-79	-0.4694856284602551
2216	80-81	-0.4290652754205482
2216	82-83	-0.4253144577645358
2216	84-85	-0.5336266652170067
2216	86-87	-0.5020059685564391
2216	88-89	-0.605907960548798
2216	90-91	-0.5970049475313601
2216	92-93	-0.5533805851666571
2216	94-95	-0.4316782547528888
2216	96-97	-0.5468175689488959
2216	98-99	-0.6278248878460175
2216	100	-0.6459430523204013
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	9.0
4	15.0
5	32.0
6	111.0
7	257.0
8	541.0
9	1204.0
10	2028.0
11	3276.0
12	5560.0
13	8432.0
14	10001.0
15	12881.0
16	15296.0
17	22473.0
18	25833.0
19	13186.0
20	8897.0
21	8632.0
22	8949.0
23	9544.0
24	11090.0
25	13581.0
26	17241.0
27	22692.0
28	29008.0
29	37714.0
30	48336.0
31	62402.0
32	80549.0
33	105734.0
34	147319.0
35	222941.0
36	404953.0
37	927735.0
38	1806128.0
39	949717.0
40	3241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24209730558565	20.337637244909892	13.681856219939117	23.738409229565338
2	15.126782901621185	20.866386072142042	40.672211430549474	23.334619595687293
3	17.504433464549223	26.526887964453827	30.758228928239788	25.210449642757162
4	11.781891119113434	17.32683840311414	40.05791865315852	30.833351824613903
5	11.917964920789261	38.49348237995954	36.58524891689985	13.003303782351352
6	29.629860764408946	39.41225067161163	18.118160061495058	12.839728502484368
7	25.928700274290673	33.31630201889426	23.66594585706177	17.089051849753297
8	24.52542360121358	36.482839014699	21.082508478363977	17.909228905723445
9	25.125560542558638	16.176453398817053	21.80233439292015	36.89565166570416
10-11	23.26908962997604	26.84971613312625	29.946323192685842	19.93487104421187
12-13	24.355169274689874	24.443419747937867	29.918237803772712	21.283173173599547
14-15	21.489906689402442	26.246490624859465	27.340110085068975	24.923492600669118
16-17	20.499677319178634	29.699572516013934	27.905605830146563	21.895144334660873
18-19	20.34279276071044	28.318480717189036	30.57877011165539	20.75995641044513
20-21	21.59405012001829	27.672199896821514	30.131594170970395	20.6021558121898
22-23	21.1924687601658	27.43792943999499	30.25261502483773	21.116986775001475
24-25	21.02676318285492	27.65821778549335	30.11286252386696	21.202156507784775
26-27	20.820808713757355	27.62022470863884	30.245810920269673	21.313155657334136
28-29	20.872573909379195	27.63881760842563	30.27729594330873	21.211312538886443
30-31	21.070781582215233	28.135765110877426	29.860282877495564	20.933170429411778
32-33	20.832857031063494	27.755957575583473	30.334844623100444	21.07634077025259
34-35	21.062542037596348	27.572492875277486	30.206089470863674	21.158875616262495
36-37	21.29977523815939	27.970783932450598	29.646284891824077	21.083155937565937
38-39	21.123214857064742	28.10518537156035	29.739607117491307	21.031992653883606
40-41	20.96688093188779	27.83015676582777	29.875154542476512	21.327807759807932
42-43	21.050734363995314	27.76747798195764	29.728730585935992	21.453057068111058
44-45	21.19887510116264	27.899838833179956	29.619628474323754	21.281657591333644
46-47	21.313970201473385	28.219210649095157	29.267607252209725	21.19921189722173
48-49	21.35739708156309	28.10086645739084	29.051532768770684	21.490203692275383
50-51	21.371057133489117	28.09007907773352	28.830198323349972	21.70866546542739
52-53	21.368154743921078	28.151514641218096	28.892059834791628	21.5882707800692
54-55	21.47841583020724	28.48398188037202	28.686703390644798	21.350898898775945
56-57	21.30952251234011	28.120261948087837	28.77879730127299	21.791418238299062
58-59	21.18741412938714	27.86778377296776	29.179970936481254	21.764831161163848
60-61	21.498366043825094	28.166769521541575	28.785245955227918	21.549618479405417
62-63	21.254872398869555	28.10387781038976	28.85770663676446	21.783543153976222
64-65	21.29023598507399	27.86440590661042	28.9466009848313	21.898757123484295
66-67	21.16353551931189	27.955524684778638	28.854116559988146	22.026823235921327
68-69	21.598963061800745	28.09793459305277	28.658364623250577	21.64473772189591
70-71	21.5590870287305	27.870023613375405	28.697284214184155	21.873605143709945
72-73	21.335319644310466	28.055850088003126	28.794545391148336	21.814284876538075
74-75	21.48246809767619	28.118036542639302	28.56723417900011	21.83226118068439
76-77	21.53393978422663	28.089883744922922	28.45819998932158	21.917976481528864
78-79	21.511451561297378	28.19987459299682	28.554936885409106	21.7337369602967
80-81	21.483051728902947	27.995449290895706	28.64123592266766	21.880263057533682
82-83	21.34596571252871	28.034576323838177	28.752983125701576	21.86647483793153
84-85	21.274277795332996	28.047315883995548	28.575134743188052	22.1032715774834
86-87	21.472280149252963	28.111437694971293	28.481885624238984	21.934396531536763
88-89	21.24311655325923	28.013248313252177	28.57735661776724	22.166278515721352
90-91	21.161647335562783	27.96641977891545	28.642466060239897	22.229466825281868
92-93	21.309685050687698	28.05678187127893	28.458196306646432	22.175336771386934
94-95	21.101025213906517	27.8500166021731	28.66277024098114	22.38618794293924
96-97	21.11301522520827	27.840387910966708	28.63165298015869	22.414943883666332
98-99	21.115542189787153	27.906296009285285	28.491916233891086	22.486245567036473
100	21.135517818987967	27.791974942373205	28.4499555726375	22.62255166600133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	38.5
2	144.0
3	232.5
4	162.0
5	141.0
6	308.5
7	716.0
8	1283.5
9	1512.5
10	1278.0
11	1122.5
12	1270.0
13	1526.5
14	1850.5
15	2208.0
16	2614.0
17	3033.5
18	3586.0
19	4330.0
20	5412.0
21	6871.0
22	9174.0
23	13188.0
24	17953.5
25	23855.0
26	32797.5
27	44590.0
28	59829.5
29	76095.5
30	91192.5
31	106921.0
32	123189.0
33	138914.0
34	153806.0
35	166222.5
36	175717.5
37	184917.0
38	192027.5
39	194703.5
40	194894.5
41	192610.5
42	187517.5
43	184978.0
44	181775.5
45	176157.0
46	171829.0
47	165210.5
48	159065.5
49	154321.0
50	148339.5
51	141128.5
52	131091.5
53	122459.5
54	116749.5
55	111037.0
56	100943.0
57	88901.0
58	79467.5
59	71043.5
60	62971.5
61	54488.5
62	45944.5
63	37620.0
64	30223.0
65	23740.0
66	18366.0
67	13792.5
68	10321.5
69	7703.5
70	5454.0
71	3832.0
72	2794.5
73	2024.0
74	1437.5
75	989.0
76	627.0
77	408.5
78	258.5
79	150.0
80	74.0
81	33.0
82	20.5
83	10.5
84	4.0
85	2.5
86	1.0
87	0.5
88	1.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047290129002796395
2	0.2437611006495211
3	0.0031302221962471012
4	0.0027934261371572234
5	3.36796059089878E-4
6	9.905766443819942E-5
7	0.0
8	2.179268617640387E-4
9	0.0
10-11	8.023670819494151E-4
12-13	0.0
14-15	2.7736146042695834E-4
16-17	1.485864966572991E-4
18-19	5.05194088634817E-4
20-21	1.9811532887639882E-5
22-23	5.943459866291964E-5
24-25	0.0010401054766010938
26-27	1.2877496376965925E-4
28-29	3.070787597584182E-4
30-31	1.7830379598875895E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	8.915189799437947E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.9811532887639882E-5
68-69	2.4764416109549853E-4
70-71	3.9623065775279764E-5
72-73	3.36796059089878E-4
74-75	1.8820956243257886E-4
76-77	9.905766443819941E-6
78-79	0.0
80-81	0.0
82-83	1.3868073021347917E-4
84-85	0.0
86-87	5.349113879662768E-4
88-89	0.0025754992753931846
90-91	0.0025655935089493646
92-93	7.627440161741355E-4
94-95	6.537805852921161E-4
96-97	2.971729933145982E-4
98-99	2.0802109532021875E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5047565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.20234686309057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5608807382066	39.503285603387376
2	15.636594095243986	17.263533820060246
3	5.625910540647202	9.316903952565728
4	2.5707297638621656	5.6764126446396075
5	1.3831977021365824	3.8177879666786727
6	0.8195450428248001	2.7144485834364622
7	0.5372313954567868	2.075950368642341
8	0.3507141669331318	1.5488196074274074
9	0.25470390695751594	1.265422807732783
>10	1.169641951192173	11.189749737136552
>50	0.06254453824707495	2.349143130585473
>100	0.027121262657783	2.5214238155253517
>500	0.0010412724023318423	0.3907295751629523
>1k	7.18116158510998E-5	0.09480311902147816
>5k	7.181161585109968E-5	0.2715852679975522
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7765	0.15383655287252368	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5937	0.11762107075391798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.9811532887639882E-5	0.0	0.0	0.0
2	0.0	1.9811532887639882E-5	0.0	0.0	0.0
3	0.0	1.9811532887639882E-5	0.0	0.0	0.0
4	0.0	1.9811532887639882E-5	0.0	0.0	0.0
5	0.0	1.9811532887639882E-5	0.0	0.0	0.0
6	0.0	1.9811532887639882E-5	0.0	0.0	0.0
7	0.0	1.9811532887639882E-5	0.0	0.0	0.0
8	0.0	1.9811532887639882E-5	0.0	0.0	0.0
9	0.0	1.9811532887639882E-5	0.0	0.0	0.0
10-11	0.0	2.9717299331459823E-5	0.0	0.0	0.0
12-13	0.0	3.9623065775279764E-5	0.0	0.0	0.0
14-15	0.0	3.9623065775279764E-5	0.0	1.9811532887639882E-5	0.0
16-17	0.0	3.9623065775279764E-5	0.0	5.9434598662919646E-5	0.0
18-19	0.0	5.9434598662919646E-5	0.0	7.924613155055953E-5	0.0
20-21	0.0	5.9434598662919646E-5	0.0	9.90576644381994E-5	0.0
22-23	0.0	7.924613155055953E-5	0.0	1.0896343088201935E-4	0.0
24-25	0.0	9.90576644381994E-5	0.0	1.8820956243257886E-4	0.0
26-27	0.0	9.90576644381994E-5	0.0	2.674556939831384E-4	0.0
28-29	0.0	1.0896343088201935E-4	0.0	7.825555490617753E-4	0.0
30-31	0.0	1.1886919732583929E-4	0.0	0.003853343146645957	0.0
32-33	0.0	1.1886919732583929E-4	0.0	0.011391631410392932	0.0
34-35	0.0	1.1886919732583929E-4	0.0	0.02198089573883645	0.0
36-37	0.0	1.1886919732583929E-4	0.0	0.04096034424519546	0.0
38-39	0.0	1.1886919732583929E-4	0.0	0.07354041007891923	0.0
40-41	0.0	1.1886919732583929E-4	0.0	0.11139034366075523	0.0
42-43	0.0	1.1886919732583929E-4	0.0	0.15098369213670354	0.0
44-45	0.0	1.3868073021347917E-4	0.0	0.19969034574096617	0.0
46-47	0.0	1.5849226310111906E-4	0.0	0.2605216574724645	0.0
48-49	0.0	1.5849226310111906E-4	0.0	0.32857427294150743	0.0
50-51	0.0	1.5849226310111906E-4	0.0	0.40289723856948845	0.0
52-53	0.0	1.5849226310111906E-4	0.0	0.476695198575947	0.0
54-55	0.0	1.5849226310111906E-4	0.0	0.5586456043656693	0.0
56-57	0.0	2.0802109532021875E-4	0.0	0.6545928581405094	0.0
58-59	0.0	2.179268617640387E-4	0.0	0.7642992215058151	0.0
60-61	0.0	2.179268617640387E-4	0.0	0.8840302205122668	0.0
62-63	0.0	2.2783262820785864E-4	0.0	1.009882983180999	0.0
64-65	0.0	2.3773839465167859E-4	0.0	1.1453740565995683	0.0
66-67	0.0	2.3773839465167859E-4	0.0	1.296347842969828	0.0
68-69	0.0	2.5754992753931845E-4	0.0	1.4718780243543175	0.0
70-71	0.0	2.5754992753931845E-4	0.0	1.6606224981748627	0.0
72-73	0.0	2.5754992753931845E-4	0.0	1.8595302883667668	0.0
74-75	0.0	2.5754992753931845E-4	0.0	2.0649362613458173	0.0
76-77	0.0	2.971729933145982E-4	0.0	2.2903023537091647	0.0
78-79	0.0	3.169845262022381E-4	0.0	2.548506061833775	0.0
80-81	0.0	3.169845262022381E-4	0.0	2.8290076502234247	0.0
82-83	0.0	3.169845262022381E-4	0.0	3.125348559156742	0.0
84-85	0.0	3.2689029264605803E-4	0.0	3.4229276888955367	0.0
86-87	0.0	3.566075919775179E-4	0.0	3.7403282572884153	0.0
88	0.0	3.566075919775179E-4	0.0	4.001790962573042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6420	0.0	54.544445	1
GTATCAA	12595	0.0	44.791203	1
TCAACGC	15185	0.0	36.490585	4
ATCAACG	15300	0.0	36.309185	3
CAACGCA	15500	0.0	35.71833	5
AACGCAG	16320	0.0	34.038513	6
TATCAAC	16460	0.0	33.89037	2
ACGCAGA	18565	0.0	29.719833	7
CGCAGAG	18665	0.0	29.40953	8
GTACATG	13755	0.0	26.554482	1
GCAGAGT	21015	0.0	25.762993	9
TACATGG	13945	0.0	25.102945	2
ACATGGG	14225	0.0	23.689579	3
GAGTACT	14270	0.0	21.621195	12-13
AGAGTAC	19435	0.0	21.34044	10-11
TAGACCG	180	5.673288E-6	20.88791	5
CATGGGG	9170	0.0	20.808392	4
CAGAGTA	20665	0.0	20.797997	10-11
AGTACTT	15275	0.0	19.491009	12-13
ATACCGC	465	0.0	19.203213	6
>>END_MODULE