FastQCFastQC Report
Wed 25 May 2016
SRR1033064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657675
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48050.031.9963151
TCAACGC45750.031.855654
ATCAACG47250.031.0433563
GGTATCA33450.029.797881
CAACGCA50150.029.154885
AACGCAG51500.028.5665556
GTACATG94200.027.5008621
TATCAAC57850.025.7615382
TACATGG97400.025.3888282
ACGCAGA59800.023.894897
ACATGGG96150.023.8608783
CGCAGAG61100.022.9465878
GAGTACA68100.019.4001371
CATGGGG70700.019.283884
GCAGAGT75100.017.5441599
GTCTAGC6550.017.2272341
CCGGTCG3301.3616955E-817.111259
AGTACAT70300.016.5180022
TCTATAC15250.016.3388653
ACGACCG1750.002325476116.1188875
Produced by FastQC (version 0.11.5)