FastQCFastQC Report
Wed 25 May 2016
SRR1033064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657675
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58460.1598283062327845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.12972721742637058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.047.9095881
GTATCAA89200.041.2221531
TCAACGC105550.034.507894
ATCAACG107450.033.8986243
CAACGCA109150.033.4128045
TATCAAC111900.032.5932162
AACGCAG113100.032.3285336
ACGCAGA132850.027.4163197
CGCAGAG134600.026.9551188
GTACATG92350.024.6043011
GCAGAGT150450.023.7092939
TACATGG96350.022.3990732
ACATGGG93150.021.8974553
GTGGTAT25400.020.4154911
GAGTACT103250.020.2554512-13
AGAGTAC138500.019.90156410-11
CAGAGTA154650.018.81090710-11
CATGGGG66200.018.5292344
TATACTG22600.018.299925
AGTACTT110500.017.96951712-13
Produced by FastQC (version 0.11.5)