##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033064_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3657675 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.683590532237005 34.0 31.0 34.0 31.0 34.0 2 32.676812729397774 34.0 31.0 34.0 31.0 34.0 3 32.78272235778192 34.0 31.0 34.0 31.0 34.0 4 36.28887859090816 37.0 37.0 37.0 35.0 37.0 5 36.272941691101586 37.0 37.0 37.0 35.0 37.0 6 36.33767352211446 37.0 37.0 37.0 35.0 37.0 7 36.2856169561265 37.0 37.0 37.0 35.0 37.0 8 36.2911614618576 37.0 37.0 37.0 35.0 37.0 9 38.09087248046915 39.0 39.0 39.0 35.0 39.0 10-11 38.04886478432337 39.0 38.5 39.0 35.0 39.0 12-13 38.02394553917448 39.0 38.0 39.0 35.0 39.0 14-15 39.4981419891051 41.0 39.0 41.0 36.5 41.0 16-17 39.496595378211566 41.0 39.0 41.0 36.0 41.0 18-19 39.51712590648431 41.0 39.5 41.0 36.5 41.0 20-21 39.51342874913709 41.0 39.5 41.0 37.0 41.0 22-23 39.44399105989461 41.0 39.0 41.0 36.0 41.0 24-25 39.39108354350783 41.0 39.0 41.0 36.0 41.0 26-27 39.28170231089422 41.0 39.0 41.0 36.0 41.0 28-29 39.23052799934385 41.0 39.0 41.0 36.0 41.0 30-31 39.23070857853691 41.0 39.0 41.0 36.0 41.0 32-33 39.19616436670836 41.0 39.0 41.0 35.0 41.0 34-35 39.119147545913734 41.0 39.0 41.0 35.0 41.0 36-37 39.03473941779956 41.0 39.0 41.0 35.0 41.0 38-39 38.942926449178785 41.0 39.0 41.0 35.0 41.0 40-41 38.847612075977224 41.0 39.0 41.0 35.0 41.0 42-43 38.73442979488336 41.0 39.0 41.0 35.0 41.0 44-45 38.571785902246646 41.0 38.0 41.0 35.0 41.0 46-47 38.38108675593102 40.5 38.0 41.0 34.5 41.0 48-49 38.18272823583287 40.0 38.0 41.0 34.0 41.0 50-51 38.052123685127846 40.0 38.0 41.0 34.0 41.0 52-53 37.92554505252654 40.0 38.0 41.0 34.0 41.0 54-55 37.78274381950283 40.0 37.5 41.0 33.0 41.0 56-57 37.63395558654063 40.0 37.0 41.0 33.0 41.0 58-59 37.45692441236578 40.0 37.0 41.0 33.0 41.0 60-61 37.266315487297256 40.0 36.0 41.0 33.0 41.0 62-63 37.039293403596545 39.0 36.0 41.0 32.5 41.0 64-65 36.77421148680514 39.0 35.0 41.0 32.0 41.0 66-67 36.495102489969725 39.0 35.0 41.0 32.0 41.0 68-69 36.18387992372203 38.0 35.0 40.5 31.0 41.0 70-71 35.851054289951946 37.0 35.0 40.0 31.0 41.0 72-73 35.505921931281485 37.0 35.0 39.5 31.0 41.0 74-75 35.1550807548511 36.0 35.0 39.0 31.0 41.0 76-77 34.79519899936435 36.0 35.0 39.0 30.5 40.5 78-79 34.42612930345096 35.0 34.5 37.5 30.0 39.5 80-81 34.09168296800563 35.0 34.0 37.0 30.0 39.0 82-83 33.78797938581202 35.0 34.0 37.0 30.0 39.0 84-85 33.55707874537787 35.0 34.0 36.0 30.0 37.5 86-87 33.34361418113966 35.0 34.0 36.0 30.0 37.0 88-89 33.12931479696802 35.0 34.0 35.5 30.0 37.0 90-91 32.94141989105101 35.0 34.0 35.0 29.5 36.0 92-93 32.767133083174414 35.0 34.0 35.0 29.0 36.0 94-95 32.60773223427451 35.0 34.0 35.0 29.0 36.0 96-97 32.45926373994409 35.0 34.0 35.0 29.0 36.0 98-99 32.332208028324004 35.0 34.0 35.0 29.0 35.5 100 32.22271798341843 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09856601130119458 1101 2 0.11292164050347964 1101 3 0.16281781021243802 1101 4 0.1083055646519 1101 5 0.07978700412513007 1101 6 0.11756336946727686 1101 7 0.15701699571734906 1101 8 0.15367048121070326 1101 9 0.1699012372433657 1101 10-11 0.21874729241392288 1101 12-13 0.2259922345582126 1101 14-15 0.2992779201629574 1101 16-17 0.26100463160948806 1101 18-19 0.2619052789843508 1101 20-21 0.2122624898504739 1101 22-23 0.2373199869288598 1101 24-25 0.29798554913716657 1101 26-27 0.280804121214949 1101 28-29 0.3008910477829545 1101 30-31 0.2600092733334165 1101 32-33 0.21291955082917013 1101 34-35 0.2545088275220522 1101 36-37 0.22014991758933178 1101 38-39 0.21823547609957217 1101 40-41 0.2595561085259064 1101 42-43 0.15346512963332515 1101 44-45 0.17592100956882462 1101 46-47 0.22621273360819316 1101 48-49 0.2152483428121741 1101 50-51 0.23698708027148285 1101 52-53 0.2127646090739077 1101 54-55 0.24304178746773886 1101 56-57 0.21608867415518063 1101 58-59 0.19181832371869945 1101 60-61 0.19929049801544352 1101 62-63 0.21255812542435848 1101 64-65 0.21037032008333512 1101 66-67 0.30108941628891017 1101 68-69 0.31919720045859634 1101 70-71 0.22600421503118184 1101 72-73 0.27502589353660767 1101 74-75 0.2746213770424575 1101 76-77 0.28221076121878497 1101 78-79 0.24509581764150568 1101 80-81 0.2275079707458687 1101 82-83 0.2535241771934693 1101 84-85 0.270760630009768 1101 86-87 0.3146241632020974 1101 88-89 0.3066349505669166 1101 90-91 0.2964800943583725 1101 92-93 0.2599357011005736 1101 94-95 0.24467292950961905 1101 96-97 0.3010604118283027 1101 98-99 0.2886404549747965 1101 100 0.27882581695640596 1102 1 0.17612917291570795 1102 2 0.14169522174566396 1102 3 0.17715295315611712 1102 4 0.1429638744913646 1102 5 0.1790204438604448 1102 6 0.14800870447818681 1102 7 0.1797784766465398 1102 8 0.18364555052732356 1102 9 0.2234024061118518 1102 10-11 0.23674165388553092 1102 12-13 0.23525406109968117 1102 14-15 0.35715263765404615 1102 16-17 0.304376642451615 1102 18-19 0.3042115225283908 1102 20-21 0.29955560789454694 1102 22-23 0.2584528185418975 1102 24-25 0.3138454635495833 1102 26-27 0.36886263453849466 1102 28-29 0.33481774691388466 1102 30-31 0.32813935558137075 1102 32-33 0.3820204540810366 1102 34-35 0.3578336952962289 1102 36-37 0.29429909006150723 1102 38-39 0.3630477969830892 1102 40-41 0.3302211985792596 1102 42-43 0.260734637310577 1102 44-45 0.26227650868732155 1102 46-47 0.30533833454639137 1102 48-49 0.31741812775590716 1102 50-51 0.28677272299382395 1102 52-53 0.2925503682947692 1102 54-55 0.24208533461469273 1102 56-57 0.25483666455569676 1102 58-59 0.24000230594878502 1102 60-61 0.220231301233909 1102 62-63 0.2588437642634105 1102 64-65 0.3196004978600797 1102 66-67 0.2838089965835735 1102 68-69 0.3743675989612001 1102 70-71 0.33402028406043627 1102 72-73 0.3043858506651489 1102 74-75 0.31475628232915653 1102 76-77 0.2294012052334793 1102 78-79 0.3005331919201879 1102 80-81 0.2874501486468759 1102 82-83 0.276604281576148 1102 84-85 0.305612327393213 1102 86-87 0.3003039709018722 1102 88-89 0.32434330817076784 1102 90-91 0.25686496663715275 1102 92-93 0.36372462131462413 1102 94-95 0.42311740243147966 1102 96-97 0.38755512667876246 1102 98-99 0.3558684503459233 1102 100 0.3496386659665589 1103 1 0.0887681537317988 1103 2 0.027592318411777228 1103 3 0.08266215158170809 1103 4 0.08160700646102015 1103 5 0.08626839695496358 1103 6 0.10672732627721615 1103 7 0.09919490151238364 1103 8 0.09798831827784937 1103 9 0.12737834621171373 1103 10-11 0.1578087956836356 1103 12-13 0.14279298097522286 1103 14-15 0.21169981111425074 1103 16-17 0.20519311843857224 1103 18-19 0.18818773622359686 1103 20-21 0.21719377542034835 1103 22-23 0.2074927986931172 1103 24-25 0.18753123141929962 1103 26-27 0.18747892418077328 1103 28-29 0.27551779121384357 1103 30-31 0.22775469176259122 1103 32-33 0.2135891036518629 1103 34-35 0.24316561143063353 1103 36-37 0.19646534683486294 1103 38-39 0.22440991204754823 1103 40-41 0.169291982568609 1103 42-43 0.18733605086156757 1103 44-45 0.19936391378158902 1103 46-47 0.2083781850209121 1103 48-49 0.1926013616829323 1103 50-51 0.22499972767942467 1103 52-53 0.2062916947599831 1103 54-55 0.2478121350407534 1103 56-57 0.22878343489376363 1103 58-59 0.19513321195416466 1103 60-61 0.19531922653413147 1103 62-63 0.10399801582271806 1103 64-65 0.1670900918670668 1103 66-67 0.17126388145990035 1103 68-69 0.17449294685830097 1103 70-71 0.19346590117536522 1103 72-73 0.2042654687110499 1103 74-75 0.16623572107640427 1103 76-77 0.20530570021768568 1103 78-79 0.17636681446503388 1103 80-81 0.15665176368397482 1103 82-83 0.15014309501184897 1103 84-85 0.1691912394131947 1103 86-87 0.1518303518650299 1103 88-89 0.18498030186009373 1103 90-91 0.1181892093589596 1103 92-93 0.18548433413695165 1103 94-95 0.18920419062445148 1103 96-97 0.17977429979116266 1103 98-99 0.1949055783593181 1103 100 0.189421116436435 1104 1 0.06290712445689906 1104 2 0.07033611510225057 1104 3 0.09295801787670399 1104 4 0.06946794048217697 1104 5 0.06881240742028893 1104 6 0.06996552966724323 1104 7 0.10090964968105709 1104 8 0.12575720695200943 1104 9 0.13810925522280115 1104 10-11 0.16111152209235513 1104 12-13 0.1335632948030323 1104 14-15 0.15800979000690063 1104 16-17 0.16489986348476293 1104 18-19 0.1610994380662234 1104 20-21 0.19750048017355937 1104 22-23 0.19181943556303338 1104 24-25 0.20148272223980257 1104 26-27 0.18435032334794244 1104 28-29 0.21854925492789334 1104 30-31 0.21603343675801767 1104 32-33 0.1873143317572712 1104 34-35 0.17056992852260322 1104 36-37 0.19866023316992454 1104 38-39 0.1875980837170701 1104 40-41 0.1912925230630762 1104 42-43 0.17180824441746267 1104 44-45 0.16538819871222188 1104 46-47 0.2013072039504067 1104 48-49 0.049214500978294495 1104 50-51 0.10140886557267237 1104 52-53 0.09516243778292477 1104 54-55 0.13558424151215576 1104 56-57 0.16772287524801044 1104 58-59 0.09069562074844129 1104 60-61 0.14992879341637888 1104 62-63 0.08239378730877434 1104 64-65 0.1384167989296543 1104 66-67 0.1761224551483096 1104 68-69 0.1572320936907161 1104 70-71 0.13301309945867956 1104 72-73 0.18355649518294825 1104 74-75 0.052152404718654566 1104 76-77 0.1320745403247301 1104 78-79 0.08881150247233194 1104 80-81 0.1335036759751631 1104 82-83 0.08008590262565463 1104 84-85 0.10457481129615331 1104 86-87 0.08217042978287736 1104 88-89 0.15406191005136094 1104 90-91 0.15564949724585375 1104 92-93 0.17839838152159615 1104 94-95 0.08792696958040835 1104 96-97 0.103624646977309 1104 98-99 0.12524202188041045 1104 100 0.11019293067340641 1105 1 0.0019587857083749327 1105 2 0.04002936125559842 1105 3 0.07726745877010188 1105 4 7.653096283917193E-4 1105 5 0.008588147712544014 1105 6 0.060785176677939035 1105 7 0.08046251392186576 1105 8 0.08596562689293563 1105 9 0.11314434398916973 1105 10-11 0.10072525068879656 1105 12-13 0.14009757722821092 1105 14-15 0.14918393870893 1105 16-17 0.11713121233143653 1105 18-19 0.15278369816359572 1105 20-21 0.17646499792563475 1105 22-23 0.18631649336592915 1105 24-25 0.16525003007627959 1105 26-27 0.16860223236339067 1105 28-29 0.2000295145238482 1105 30-31 0.15438707438696753 1105 32-33 0.14343958886092167 1105 34-35 0.189808085990812 1105 36-37 0.1814588728361528 1105 38-39 0.17783871187224776 1105 40-41 0.17291827308566354 1105 42-43 0.18322617959061915 1105 44-45 0.09636340310957081 1105 46-47 0.08355283887620146 1105 48-49 0.058843440844938755 1105 50-51 0.06420042036796758 1105 52-53 0.05867850186039192 1105 54-55 0.0690032858131886 1105 56-57 0.014149538125799666 1105 58-59 0.07841751257969776 1105 60-61 0.07042567540543843 1105 62-63 0.02671320360682472 1105 64-65 0.013117411159711878 1105 66-67 0.08160945432066313 1105 68-69 0.10621403587467171 1105 70-71 0.13069230859103698 1105 72-73 0.10488981805281128 1105 74-75 0.11722846210225413 1105 76-77 0.08512484504233697 1105 78-79 0.14278975869229527 1105 80-81 0.12214795370624643 1105 82-83 0.12773011076087926 1105 84-85 0.09775866913503961 1105 86-87 0.0782232576424775 1105 88-89 0.056662178838642774 1105 90-91 0.10597057434449653 1105 92-93 0.1345780555555791 1105 94-95 0.10007170291989098 1105 96-97 0.16972670959580682 1105 98-99 0.1473821574883587 1105 100 0.1435605938273099 1106 1 0.029220790760881243 1106 2 0.0450674182657238 1106 3 0.05001816324588049 1106 4 0.07402811444468682 1106 5 0.08426917440696968 1106 6 0.08697208942247414 1106 7 0.05281467434767961 1106 8 0.054809756836760926 1106 9 0.05642232130654179 1106 10-11 0.06414027150499635 1106 12-13 0.05062239779976352 1106 14-15 0.1199731950637215 1106 16-17 0.15521220333215524 1106 18-19 0.10677005508701143 1106 20-21 0.10383924149130053 1106 22-23 0.09327786322297271 1106 24-25 0.08955013976619597 1106 26-27 0.09561903716925002 1106 28-29 0.09338941478481644 1106 30-31 0.05385631110755895 1106 32-33 0.005447992668585755 1106 34-35 0.03700734744681 1106 36-37 -0.009464731259633652 1106 38-39 0.04030388943706242 1106 40-41 0.08466648609255145 1106 42-43 0.029379881668845087 1106 44-45 0.032781713553049485 1106 46-47 0.06107373033054131 1106 48-49 0.06731815430192967 1106 50-51 0.047050718045191786 1106 52-53 0.0329237799696287 1106 54-55 0.08817490685465401 1106 56-57 0.03400566760352319 1106 58-59 -0.05953267730734524 1106 60-61 -0.016906476878261856 1106 62-63 0.0175923109751821 1106 64-65 0.08110131340497162 1106 66-67 0.06081960931403074 1106 68-69 -0.0016826014115807197 1106 70-71 0.002470727464178424 1106 72-73 0.049367942747196025 1106 74-75 0.06807616757084389 1106 76-77 0.0674530821223982 1106 78-79 0.10215739678165647 1106 80-81 0.07032154097862531 1106 82-83 0.07824449212726137 1106 84-85 0.10209114664888119 1106 86-87 0.058600348008695846 1106 88-89 0.12334964230711165 1106 90-91 0.10423488758111432 1106 92-93 0.06733786139144371 1106 94-95 0.042618046207991256 1106 96-97 0.05309377669289006 1106 98-99 0.10976202067229934 1106 100 0.11144114085075785 1107 1 0.021398501919030366 1107 2 -0.013495420336560926 1107 3 0.047548722253402786 1107 4 0.028405581815363234 1107 5 0.08669919007902394 1107 6 0.05747084310820583 1107 7 0.07538755387110996 1107 8 0.05430265108849852 1107 9 0.10199279562561969 1107 10-11 0.09582244600562007 1107 12-13 0.09895030630860191 1107 14-15 0.11812244581700782 1107 16-17 0.09705426991521904 1107 18-19 0.14022471275345083 1107 20-21 0.1611008821419091 1107 22-23 0.17857115026001935 1107 24-25 0.1413821278863736 1107 26-27 0.1257457139063618 1107 28-29 0.1516307291821306 1107 30-31 0.17135805520708658 1107 32-33 0.18064107768821458 1107 34-35 0.14863177976604192 1107 36-37 0.19255502725605567 1107 38-39 0.19713955151647156 1107 40-41 0.1411315014466652 1107 42-43 0.10745606178006994 1107 44-45 0.14419373952283365 1107 46-47 0.14676612566687197 1107 48-49 0.10781922739160166 1107 50-51 0.09617992833003797 1107 52-53 0.1236436343596381 1107 54-55 0.12916891011402498 1107 56-57 0.08624103455810683 1107 58-59 0.09156574004857987 1107 60-61 0.1081330052743823 1107 62-63 0.12036309586446947 1107 64-65 0.10270262183198042 1107 66-67 0.0652681266978945 1107 68-69 0.11941842438069727 1107 70-71 0.09261310158422731 1107 72-73 0.1501925976928291 1107 74-75 0.14852491410221802 1107 76-77 0.1314613076601674 1107 78-79 0.11492983662507328 1107 80-81 0.16051477181348162 1107 82-83 0.10521131386902738 1107 84-85 0.18489437071777814 1107 86-87 0.14139014343675171 1107 88-89 0.14185731338977803 1107 90-91 0.15488807810602623 1107 92-93 0.1843373873133416 1107 94-95 0.194586223804059 1107 96-97 0.23728804557629957 1107 98-99 0.22013276677278526 1107 100 0.19492347743037186 1108 1 -0.03775946594491586 1108 2 -0.022151881884902025 1108 3 -0.011022148929846765 1108 4 -0.044705418502751115 1108 5 -0.007760909054333354 1108 6 -0.048594373320149487 1108 7 -0.035353058452962216 1108 8 -0.06264606457817479 1108 9 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-0.2825286418533466 2214 54-55 -0.2814369488214936 2214 56-57 -0.28703607823040755 2214 58-59 -0.2654938949441643 2214 60-61 -0.30204171468581364 2214 62-63 -0.3792901251444434 2214 64-65 -0.32247844363686085 2214 66-67 -0.282178452510351 2214 68-69 -0.25374462854879454 2214 70-71 -0.24109617775602032 2214 72-73 -0.2670529921702425 2214 74-75 -0.3006808540341481 2214 76-77 -0.24911250906485094 2214 78-79 -0.2112107563560599 2214 80-81 -0.20836942380340417 2214 82-83 -0.21522641236465034 2214 84-85 -0.2129855826688427 2214 86-87 -0.26037649710299604 2214 88-89 -0.28084500445687866 2214 90-91 -0.2310390639224238 2214 92-93 -0.27014449767012394 2214 94-95 -0.3194759938157574 2214 96-97 -0.27543753745271715 2214 98-99 -0.33315631066216866 2214 100 -0.3170146382390584 2215 1 -0.22012871740606954 2215 2 -0.1770345251037071 2215 3 -0.20877445466071265 2215 4 -0.15230388897384728 2215 5 -0.18402496966456283 2215 6 -0.20617093439514633 2215 7 -0.20668037384271543 2215 8 -0.16920181316974237 2215 9 -0.24282347047507358 2215 10-11 -0.23674533640762974 2215 12-13 -0.2578589505636941 2215 14-15 -0.3256089810816931 2215 16-17 -0.3215159024324237 2215 18-19 -0.31547851408318905 2215 20-21 -0.34767357767506724 2215 22-23 -0.33634653922687363 2215 24-25 -0.43996225105126996 2215 26-27 -0.3865597682280466 2215 28-29 -0.4111479477646043 2215 30-31 -0.4507454585604407 2215 32-33 -0.355778314282837 2215 34-35 -0.38946494711597524 2215 36-37 -0.41209925471345343 2215 38-39 -0.4526439467015919 2215 40-41 -0.43301032993595356 2215 42-43 -0.3996659745944271 2215 44-45 -0.37842159372239337 2215 46-47 -0.4264629332855492 2215 48-49 -0.4832060055621028 2215 50-51 -0.3980837899302898 2215 52-53 -0.36536141417814605 2215 54-55 -0.43830943215461105 2215 56-57 -0.35944925062380406 2215 58-59 -0.4002351557460102 2215 60-61 -0.40047044521438124 2215 62-63 -0.3773603092336657 2215 64-65 -0.4325768151872893 2215 66-67 -0.46302013958362664 2215 68-69 -0.3810038117219463 2215 70-71 -0.34477092231198014 2215 72-73 -0.33670429159909077 2215 74-75 -0.34202963896449745 2215 76-77 -0.27710639955709127 2215 78-79 -0.29180558382959276 2215 80-81 -0.2632076125438658 2215 82-83 -0.2657496572871594 2215 84-85 -0.2503878022269461 2215 86-87 -0.299780465629496 2215 88-89 -0.24328723523119322 2215 90-91 -0.21764486126150473 2215 92-93 -0.22393847386815224 2215 94-95 -0.19352992311323192 2215 96-97 -0.2562485403490484 2215 98-99 -0.2618088663699396 2215 100 -0.20748628542587966 2216 1 -0.1980888192160677 2216 2 -0.20263237289609748 2216 3 -0.16743211109466216 2216 4 -0.17193634930684 2216 5 -0.18076824565811478 2216 6 -0.16438279047229543 2216 7 -0.17191364591975855 2216 8 -0.19064660242099052 2216 9 -0.26751271444133806 2216 10-11 -0.2575260140319031 2216 12-13 -0.2505368161629704 2216 14-15 -0.28988454801474006 2216 16-17 -0.28211831401478094 2216 18-19 -0.27837668398795756 2216 20-21 -0.34162481699909364 2216 22-23 -0.3604410848409074 2216 24-25 -0.3357716214279449 2216 26-27 -0.37765593377498163 2216 28-29 -0.36167331012894266 2216 30-31 -0.374494758872693 2216 32-33 -0.34558354749308506 2216 34-35 -0.4053987474294942 2216 36-37 -0.3419186903531042 2216 38-39 -0.3715010361203852 2216 40-41 -0.37360857962008964 2216 42-43 -0.3834564775059164 2216 44-45 -0.411276099440947 2216 46-47 -0.3062217518629211 2216 48-49 -0.331619127289116 2216 50-51 -0.3613047774517426 2216 52-53 -0.33058376948407187 2216 54-55 -0.3089227740332987 2216 56-57 -0.3215649150696933 2216 58-59 -0.3202121802901914 2216 60-61 -0.3424685292727361 2216 62-63 -0.3833301165362357 2216 64-65 -0.44754793501421375 2216 66-67 -0.45244903401613357 2216 68-69 -0.4400114955830716 2216 70-71 -0.4320084101948538 2216 72-73 -0.35698168675317277 2216 74-75 -0.45095271301904916 2216 76-77 -0.395344764066337 2216 78-79 -0.4350144199221049 2216 80-81 -0.39405818068934906 2216 82-83 -0.3807056723604205 2216 84-85 -0.4480030722199544 2216 86-87 -0.5042202874600576 2216 88-89 -0.5341612927902091 2216 90-91 -0.5028071289202529 2216 92-93 -0.44281480771886095 2216 94-95 -0.3726068975561958 2216 96-97 -0.5377937580980614 2216 98-99 -0.6603762671910758 2216 100 -0.651897120813917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27.0 3 8.0 4 16.0 5 30.0 6 69.0 7 215.0 8 479.0 9 840.0 10 1584.0 11 2533.0 12 3876.0 13 6170.0 14 7251.0 15 9411.0 16 11553.0 17 16448.0 18 18610.0 19 9904.0 20 6841.0 21 6492.0 22 6838.0 23 7473.0 24 8777.0 25 10646.0 26 13609.0 27 17785.0 28 22686.0 29 29862.0 30 38215.0 31 48612.0 32 62548.0 33 81334.0 34 113247.0 35 169833.0 36 307548.0 37 702871.0 38 1293950.0 39 617368.0 40 2116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54148603000506 20.053008027789552 13.714664733797404 23.69084120840798 2 15.45413510466949 20.73470765909946 40.102626336374634 23.708530899856417 3 17.820058372008777 26.14573863830543 30.580644279338088 25.4535587103477 4 12.01930122331851 17.285334176146492 39.464844630018874 31.23051997051612 5 12.304335392390001 38.00124232364828 36.24933085670573 13.44509142725599 6 30.05548337849867 39.0001618515821 17.90261674638951 13.04173802352972 7 26.25082873683419 32.95508758979406 23.393685879691333 17.400397793680412 8 24.830151063712282 36.20545036692838 20.816521592208357 18.14787697715098 9 25.042711558572044 16.162972379995487 21.505874633476182 37.28844142795628 10-11 23.537263145750057 26.81212049631246 29.49882786938245 20.15178848855503 12-13 24.598371916586355 24.211869561948507 29.57133971717006 21.61841880429508 14-15 21.67271241973019 25.91624052947455 27.147586547042046 25.263460503753215 16-17 20.879117273870325 29.426241138823663 27.5551724335909 22.139469153715112 18-19 20.76311136294199 28.02901857267937 30.1828834438435 21.024986620535138 20-21 21.946023620475742 27.362102254055447 29.836844398926747 20.85502972654206 22-23 21.3916792600749 27.224385486105557 29.851865977429604 21.53206927638994 24-25 21.352860893134743 27.329128611555998 29.866082828407652 21.45192766690161 26-27 21.172069999293264 27.29396660246816 29.879796279894922 21.654167118343654 28-29 21.163132763662063 27.40652848196869 29.920194987785926 21.510143766583326 30-31 21.410334801890983 27.864360663433185 29.518979361317538 21.20632517335829 32-33 21.127177783701395 27.434627188036114 29.975134477502785 21.463060550759707 34-35 21.20824020723547 27.307962025056902 29.94656441591995 21.53723335178768 36-37 21.59910325548333 27.5876205513065 29.35927877681861 21.45399741639156 38-39 21.471686248778255 27.805860280095963 29.423158153745206 21.299295317380576 40-41 21.26862386785912 27.546127700898275 29.59905918299946 21.586189248243144 42-43 21.307811656311728 27.461174106502078 29.41114232401731 21.819871913168885 44-45 21.46345697745152 27.668491596437626 29.248511691169938 21.61953973494091 46-47 21.59501595959182 27.828483941301506 28.966228546822776 21.610271552283898 48-49 21.66348841818915 27.861496715809906 28.68518936209477 21.789825503906172 50-51 21.67797849726944 27.82603703172097 28.60884304920475 21.887141421804834 52-53 21.774009445891174 27.784918014859166 28.525306376318287 21.915766162931373 54-55 21.789470086872125 28.25674779743963 28.32572604181618 21.628056073872063 56-57 21.54883908493784 27.89532968347379 28.517241143622655 22.038590087965716 58-59 21.471576889690855 27.522866301680715 28.86880326983671 22.136753538791716 60-61 21.618664862241722 27.91989446848066 28.546453689843958 21.914986979433657 62-63 21.581715160586985 27.82569528457285 28.55178494535463 22.040804609485534 64-65 21.527213325404798 27.66466402837868 28.624413049273105 22.183709596943412 66-67 21.414587135270356 27.81917474898672 28.480633189116038 22.28560492662689 68-69 21.83460882726371 27.801990229840435 28.49102214853836 21.8723787943575 70-71 21.793409993152753 27.663068433235377 28.45098709576262 22.092534477849245 72-73 21.626778508526414 27.83970481710392 28.50544384264877 22.028072831720895 74-75 21.656233801171894 27.93656730291836 28.287391543036115 22.11980735287363 76-77 21.790700376605358 27.815019103665577 28.21591584818225 22.178364671546817 78-79 21.87855673344406 27.956311044584332 28.25723991333292 21.907892308638687 80-81 21.791274511814198 27.780393282618054 28.35547171358855 22.072860491979192 82-83 21.641666485540604 27.83747647240794 28.512386357065495 22.008470684985962 84-85 21.627222210830652 27.8224145119509 28.233768719200036 22.316594558018412 86-87 21.785796012371833 27.963101029263765 28.154452720220537 22.096650238143862 88-89 21.47516148629178 27.811690552183872 28.283176390110853 22.429971571413496 90-91 21.41791842545372 27.75112389671114 28.35663239179273 22.47432528604241 92-93 21.63788480410886 27.900501318262116 28.16124204118129 22.300371836447734 94-95 21.38679506946593 27.68139927847243 28.27565956507944 22.656146086982197 96-97 21.320748088366102 27.69606439291467 28.31492839908034 22.668259119638883 98-99 21.29885294573176 27.8396363152698 28.15672311921772 22.704787619780717 100 21.449171946660105 27.585447039444453 28.077043477072184 22.888337536823254 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 31.5 2 108.5 3 199.0 4 158.0 5 123.5 6 265.0 7 582.0 8 1039.0 9 1300.5 10 1127.5 11 945.5 12 1053.0 13 1252.0 14 1471.5 15 1712.0 16 1954.0 17 2281.5 18 2718.0 19 3251.5 20 4065.5 21 5126.5 22 6643.0 23 9254.0 24 12681.0 25 16640.0 26 22144.0 27 29616.0 28 39005.0 29 49196.5 30 58953.5 31 69426.5 32 80459.0 33 91690.0 34 102706.5 35 111513.0 36 118892.5 37 126066.5 38 130810.0 39 132734.5 40 134256.0 41 134988.0 42 134815.5 43 134151.0 44 131751.0 45 130968.0 46 129650.5 47 123999.0 48 120381.0 49 118550.5 50 115452.5 51 111088.0 52 103002.0 53 97369.5 54 95189.5 55 89804.5 56 80822.0 57 72805.0 58 64434.0 59 54964.5 60 47522.5 61 40276.0 62 33814.0 63 27785.5 64 21980.5 65 17383.5 66 13373.0 67 9820.5 68 7498.0 69 5979.5 70 4456.5 71 3042.5 72 2278.5 73 1843.0 74 1299.5 75 731.5 76 390.0 77 248.5 78 154.5 79 71.0 80 35.0 81 16.5 82 8.5 83 6.5 84 3.5 85 1.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044427129255606365 2 0.24343332854887328 3 0.0027339771849603915 4 0.002269201063517125 5 3.554170340448509E-4 6 8.201931554881175E-5 7 0.0 8 3.554170340448509E-4 9 0.0 10-11 5.331255510672763E-4 12-13 0.0 14-15 3.007374903456431E-4 16-17 1.640386310976235E-4 18-19 4.784460073680685E-4 20-21 2.7339771849603915E-5 22-23 2.0504828887202937E-4 24-25 0.0011209306458337604 26-27 2.597278325712372E-4 28-29 2.733977184960392E-4 30-31 1.230289733232176E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.201931554881175E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.913784029472274E-4 70-71 1.3669885924801958E-5 72-73 2.870676044208411E-4 74-75 8.201931554881175E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.100965777440587E-5 84-85 0.0 86-87 3.28077262195247E-4 88-89 0.0024332396946147483 90-91 0.0024058999227651446 92-93 5.057857792176725E-4 94-95 5.057857792176725E-4 96-97 1.5036874517282154E-4 98-99 4.100965777440587E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3657675.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.199428197635086 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31644427362141 45.34006879151056 2 14.626610690727073 17.610272001023723 3 4.690983365305807 8.471835488280826 4 1.931447596869439 4.650881637009465 5 1.002100285082443 3.0162932079325087 6 0.6017646044915953 2.173553105998204 7 0.3775903266673189 1.5911505230831589 8 0.27029228103258496 1.301715261151688 9 0.1988319286224757 1.0772611569455428 >10 0.9059089925311441 9.626528905913476 >50 0.05547499733416754 2.281503642095061 >100 0.021686405901126086 2.18600210106378 >500 6.823040666148179E-4 0.2821257746458148 >1k 1.3646081008494046E-4 0.23072811144865568 >5k 4.548693669498016E-5 0.1600802918975601 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5846 0.1598283062327845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.12972721742637058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.7339771849603915E-5 0.0 0.0 0.0 0.0 14-15 2.7339771849603915E-5 0.0 0.0 0.0 0.0 16-17 2.7339771849603915E-5 0.0 0.0 0.0 0.0 18-19 4.100965777440587E-5 0.0 0.0 0.0 0.0 20-21 5.467954369920783E-5 0.0 0.0 1.3669885924801958E-5 0.0 22-23 5.467954369920783E-5 0.0 0.0 2.7339771849603915E-5 0.0 24-25 5.467954369920783E-5 0.0 0.0 1.3669885924801956E-4 0.0 26-27 5.467954369920783E-5 0.0 0.0 2.1871817479683132E-4 0.0 28-29 5.467954369920783E-5 0.0 0.0 7.381738399393057E-4 0.0 30-31 5.467954369920783E-5 0.0 0.0 0.00314407376270445 0.0 32-33 5.467954369920783E-5 0.0 0.0 0.009514240603662162 0.0 34-35 5.467954369920783E-5 0.0 0.0 0.018194618165911405 0.0 36-37 5.467954369920783E-5 0.0 0.0 0.033682598918712024 0.0 38-39 5.467954369920783E-5 0.0 0.0 0.061459807117909604 0.0 40-41 8.201931554881175E-5 0.0 0.0 0.09615397759505698 0.0 42-43 8.201931554881175E-5 0.0 0.0 0.13232449575208294 0.0 44-45 8.201931554881175E-5 0.0 0.0 0.17229524219620387 0.0 46-47 8.201931554881175E-5 0.0 0.0 0.2255121081014579 0.0 48-49 8.201931554881175E-5 0.0 0.0 0.28196873697089 0.0 50-51 8.201931554881175E-5 0.0 0.0 0.34304578728290513 0.0 52-53 8.201931554881175E-5 0.0 0.0 0.40795040565386487 0.0 54-55 8.201931554881175E-5 0.0 0.0 0.47876041474433895 0.0 56-57 8.201931554881175E-5 0.0 0.0 0.5613401956160675 0.0 58-59 8.201931554881175E-5 0.0 0.0 0.6556213988394267 0.0 60-61 8.201931554881175E-5 0.0 0.0 0.7589247267731551 0.0 62-63 8.201931554881175E-5 0.0 0.0 0.8683795033730444 0.0 64-65 8.201931554881175E-5 0.0 0.0 0.9810056935074877 0.0 66-67 8.201931554881175E-5 0.0 0.0 1.1135625773202922 0.0 68-69 8.201931554881175E-5 0.0 0.0 1.263589575344994 0.0 70-71 8.201931554881175E-5 0.0 0.0 1.424948908801356 0.0 72-73 8.201931554881175E-5 0.0 0.0 1.6006479525928357 0.0 74-75 8.201931554881175E-5 0.0 0.0 1.777741324748645 0.0 76-77 8.201931554881175E-5 0.0 0.0 1.9688873396351507 0.0 78-79 8.201931554881175E-5 0.0 0.0 2.1891502115414845 0.0 80-81 8.201931554881175E-5 0.0 0.0 2.431230221383802 0.0 82-83 8.201931554881175E-5 0.0 0.0 2.685818176847314 0.0 84-85 8.201931554881175E-5 0.0 0.0 2.946051795197769 0.0 86-87 8.201931554881175E-5 0.0 0.0 3.217549399550261 0.0 88 8.201931554881175E-5 0.0 0.0 3.439944773660864 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5215 0.0 47.909588 1 GTATCAA 8920 0.0 41.222153 1 TCAACGC 10555 0.0 34.50789 4 ATCAACG 10745 0.0 33.898624 3 CAACGCA 10915 0.0 33.412804 5 TATCAAC 11190 0.0 32.593216 2 AACGCAG 11310 0.0 32.328533 6 ACGCAGA 13285 0.0 27.416319 7 CGCAGAG 13460 0.0 26.955118 8 GTACATG 9235 0.0 24.604301 1 GCAGAGT 15045 0.0 23.709293 9 TACATGG 9635 0.0 22.399073 2 ACATGGG 9315 0.0 21.897455 3 GTGGTAT 2540 0.0 20.415491 1 GAGTACT 10325 0.0 20.25545 12-13 AGAGTAC 13850 0.0 19.901564 10-11 CAGAGTA 15465 0.0 18.810907 10-11 CATGGGG 6620 0.0 18.529234 4 TATACTG 2260 0.0 18.29992 5 AGTACTT 11050 0.0 17.969517 12-13 >>END_MODULE