##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033063_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129490
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.894748629237778	31.0	27.0	34.0	12.0	34.0
2	27.91305120086493	31.0	27.0	34.0	7.0	34.0
3	28.1580971503591	33.0	27.0	34.0	10.0	34.0
4	30.927685535562592	37.0	31.0	37.0	10.0	37.0
5	31.618858599119623	37.0	32.0	37.0	12.0	37.0
6	31.34079851726002	37.0	32.0	37.0	8.0	37.0
7	31.675117769711946	37.0	32.0	37.0	8.0	37.0
8	31.14984168661673	37.0	32.0	37.0	8.0	37.0
9	32.115808170515095	39.0	32.0	39.0	8.0	39.0
10-11	32.5159703452004	39.0	32.0	39.0	8.0	39.0
12-13	32.55508147347285	39.0	32.0	39.0	7.5	39.0
14-15	33.787338790640206	40.0	32.0	41.0	8.0	41.0
16-17	33.988195999691094	40.0	32.0	41.0	8.0	41.0
18-19	33.9564059000695	40.0	32.0	41.0	8.0	41.0
20-21	33.98510695806626	40.0	32.0	41.0	11.0	41.0
22-23	33.92068885628234	40.0	32.0	41.0	13.0	41.0
24-25	33.79633176307051	40.0	31.5	41.0	10.5	41.0
26-27	33.26466522511391	40.0	31.0	41.0	2.0	41.0
28-29	32.86975055988879	39.0	30.0	41.0	2.0	41.0
30-31	32.61168816124797	40.0	30.0	41.0	2.0	41.0
32-33	32.279766777357324	40.0	30.0	41.0	2.0	41.0
34-35	31.92269287203645	40.0	30.0	41.0	2.0	41.0
36-37	31.573264344737044	39.5	30.0	41.0	2.0	41.0
38-39	31.248938141941462	39.0	30.0	41.0	2.0	41.0
40-41	31.017580508147347	39.0	29.5	41.0	2.0	41.0
42-43	30.75284964089891	39.0	28.0	41.0	2.0	41.0
44-45	30.564997297088578	39.0	28.0	41.0	2.0	41.0
46-47	30.35816279249363	38.0	27.0	41.0	2.0	41.0
48-49	30.17797513321492	38.0	27.0	41.0	2.0	41.0
50-51	29.984350142868173	38.0	27.0	41.0	2.0	41.0
52-53	29.814885319329676	38.0	27.0	41.0	2.0	41.0
54-55	29.63038072438026	37.0	26.5	41.0	2.0	41.0
56-57	29.43718819986099	37.0	26.0	40.5	2.0	41.0
58-59	29.20694262105182	36.5	26.0	40.0	2.0	41.0
60-61	28.970144412695962	36.0	25.0	40.0	2.0	41.0
62-63	28.687110973820374	35.0	25.0	40.0	2.0	41.0
64-65	28.38562051123639	35.0	24.0	39.5	2.0	41.0
66-67	28.092895204262877	35.0	23.0	39.0	2.0	41.0
68-69	27.813325353309136	35.0	22.0	39.0	2.0	41.0
70-71	27.500718202177772	35.0	20.5	37.5	2.0	40.5
72-73	27.203506062244188	35.0	19.5	37.0	2.0	40.0
74-75	26.90598115684609	35.0	18.0	36.5	2.0	39.0
76-77	26.62582824928566	35.0	14.5	36.0	2.0	39.0
78-79	26.33591010888872	34.5	8.5	35.5	2.0	38.5
80-81	26.034346281566144	34.0	4.0	35.0	2.0	37.0
82-83	25.858784462120628	34.0	2.0	35.0	2.0	37.0
84-85	25.668163564754035	34.0	2.0	35.0	2.0	36.5
86-87	25.513904548613795	34.0	2.0	35.0	2.0	36.0
88-89	25.324565603521506	34.0	2.0	35.0	2.0	36.0
90-91	25.112981697428374	34.0	2.0	35.0	2.0	36.0
92-93	24.853969418487914	34.0	2.0	35.0	2.0	35.0
94-95	24.599606147192834	34.0	2.0	35.0	2.0	35.0
96-97	24.390366051432544	34.0	2.0	35.0	2.0	35.0
98-99	24.179724303034984	34.0	2.0	35.0	2.0	35.0
100	24.032203258938914	34.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2853159524415325
1101	2	0.2847596349694115
1101	3	-0.1804498867616715
1101	4	0.3600493363816568
1101	5	-0.07457738842332517
1101	6	1.0628605945267395
1101	7	0.45547141729744567
1101	8	0.2838540245717347
1101	9	0.741884917546777
1101	10-11	0.6099702836084404
1101	12-13	1.0342426858610736
1101	14-15	1.2258513766711303
1101	16-17	1.0906116193292306
1101	18-19	1.1750397215234543
1101	20-21	1.0982517516015022
1101	22-23	1.2016713513540012
1101	24-25	1.244516393353365
1101	26-27	0.4101656724210443
1101	28-29	0.4208042569487205
1101	30-31	0.15963221661545646
1101	32-33	0.1997132382142226
1101	34-35	0.12684246024580403
1101	36-37	-0.051756547765322125
1101	38-39	0.18144253895236062
1101	40-41	0.23435682071865926
1101	42-43	0.33486403814889343
1101	44-45	0.3109047580444191
1101	46-47	0.4366850275668881
1101	48-49	0.29233405238385757
1101	50-51	0.2939294805400188
1101	52-53	-0.03598752834178498
1101	54-55	-0.3562256550789087
1101	56-57	-0.4223615582005458
1101	58-59	-0.20247729574864337
1101	60-61	-0.12244261854290173
1101	62-63	-0.34296806166246796
1101	64-65	-0.82607843000104
1101	66-67	-0.7811646108913095
1101	68-69	-0.6146030354602736
1101	70-71	-0.7235667587435906
1101	72-73	-0.6367740175296177
1101	74-75	-0.7197665340182873
1101	76-77	-0.7267610195137486
1101	78-79	-0.7765607332562219
1101	80-81	-0.7365581390195501
1101	82-83	-0.6588596891167882
1101	84-85	-0.4612229834031858
1101	86-87	-0.388238853625797
1101	88-89	-0.27134108049725114
1101	90-91	-0.06581602691014155
1101	92-93	0.030047849943677818
1101	94-95	-0.05441677526752997
1101	96-97	0.10192317987139532
1101	98-99	0.3451245005538581
1101	100	-0.2739232684037951
1102	1	0.4469574308195057
1102	2	0.25640410601534924
1102	3	-0.11865129331561519
1102	4	0.32619478004468405
1102	5	0.6354704942640907
1102	6	-0.48625656842402876
1102	7	-0.3101278644622454
1102	8	-0.4739269121333365
1102	9	-0.3258616034767172
1102	10-11	0.044187700935985674
1102	12-13	-0.38777960951525614
1102	14-15	-0.6536937377984202
1102	16-17	-0.6843584539911376
1102	18-19	-0.6363099717280747
1102	20-21	-0.6060539494309722
1102	22-23	-0.8981341252283741
1102	24-25	-0.4077355892891603
1102	26-27	-0.21648029495887755
1102	28-29	0.43008152763553653
1102	30-31	0.7025582977167559
1102	32-33	-0.025915222058110032
1102	34-35	-0.03992396201963544
1102	36-37	-0.3201053432164649
1102	38-39	-0.029110356453895037
1102	40-41	-0.41617437784485745
1102	42-43	-0.40868302155483605
1102	44-45	-0.4737727819825146
1102	46-47	-0.42503500834512664
1102	48-49	-0.8015609049706249
1102	50-51	-0.4977060125020252
1102	52-53	-0.5360062325165593
1102	54-55	-0.5893731936095072
1102	56-57	-0.38721641380431393
1102	58-59	-0.19776384370614508
1102	60-61	-0.3794753882571058
1102	62-63	-0.3210841771794506
1102	64-65	-0.5176278838391362
1102	66-67	-0.19202051392886688
1102	68-69	-0.0872575319438873
1102	70-71	-0.4568265223228174
1102	72-73	-0.28545350279073034
1102	74-75	-0.37985556589019964
1102	76-77	-0.42263113772413163
1102	78-79	-0.44998584174043543
1102	80-81	-0.38405926127003553
1102	82-83	-0.34683664557346816
1102	84-85	-0.047729791722805004
1102	86-87	-0.4630929610625749
1102	88-89	-0.4205442826519743
1102	90-91	-0.5034376040461588
1102	92-93	-0.1291620857139577
1102	94-95	0.27803122712660766
1102	96-97	0.4770915174443431
1102	98-99	0.14070657447275892
1102	100	-0.07188077251871405
1103	1	0.5365921861341008
1103	2	0.2322922498304436
1103	3	0.37428635827519585
1103	4	1.081830391401514
1103	5	0.36203633817905967
1103	6	0.8131442020139801
1103	7	0.7714101580802009
1103	8	1.5463327540101943
1103	9	1.3078236583295322
1103	10-11	0.7889266080554052
1103	12-13	1.2453884601095169
1103	14-15	1.4608910817193461
1103	16-17	1.506663803106882
1103	18-19	0.9337606517560104
1103	20-21	0.7343833454755782
1103	22-23	0.8188012435831595
1103	24-25	0.9627523547827366
1103	26-27	1.4953188204641492
1103	28-29	1.6208184373230807
1103	30-31	1.6190763459404636
1103	32-33	1.7038964486509869
1103	34-35	1.6644204523048032
1103	36-37	1.7966552503061521
1103	38-39	1.7544714669160584
1103	40-41	1.9046631168048762
1103	42-43	2.003965002414347
1103	44-45	1.780204247550941
1103	46-47	1.7458880905277567
1103	48-49	1.5710493104666732
1103	50-51	1.4758636836029844
1103	52-53	1.5310147405181134
1103	54-55	1.4840324277344799
1103	56-57	1.258863626144322
1103	58-59	1.2472532771384834
1103	60-61	1.2855267518484759
1103	62-63	1.366121558303501
1103	64-65	1.1988081270040674
1103	66-67	0.8947429330678531
1103	68-69	1.1548240774155047
1103	70-71	1.1785886581594163
1103	72-73	1.0689123125894966
1103	74-75	0.9968386843594779
1103	76-77	1.2058822022673041
1103	78-79	1.1545226473450256
1103	80-81	1.31775269478646
1103	82-83	1.2139423528571207
1103	84-85	1.322526307578098
1103	86-87	1.278096937639095
1103	88-89	1.2173855218850491
1103	90-91	1.4205708137024509
1103	92-93	1.1295231760922917
1103	94-95	0.7740006949536884
1103	96-97	1.2198329926904563
1103	98-99	1.0867580796803473
1103	100	0.8454754837232592
1104	1	-1.01306469529937
1104	2	-0.8255295826768645
1104	3	-1.0787572304908473
1104	4	-1.077942133696979
1104	5	-0.42440412439560404
1104	6	-1.4802221723665028
1104	7	-0.5441953826492458
1104	8	-0.8262685930478817
1104	9	-1.5105141228370726
1104	10-11	-1.025392238049566
1104	12-13	-0.9821340967592107
1104	14-15	-0.9957266587813578
1104	16-17	-1.0318893802709255
1104	18-19	-0.9137580926841977
1104	20-21	-0.6108479418544519
1104	22-23	-1.183041430200042
1104	24-25	-1.460268359412197
1104	26-27	-1.4957219725209825
1104	28-29	-1.4711722857675298
1104	30-31	-1.3673736477054845
1104	32-33	-1.5884215078873325
1104	34-35	-1.4962416394476463
1104	36-37	-1.4420504302123405
1104	38-39	-1.4601841437079095
1104	40-41	-1.1498228407500832
1104	42-43	-1.3084952847885987
1104	44-45	-1.226546927947851
1104	46-47	-1.1603261679549028
1104	48-49	-1.3262851665952162
1104	50-51	-1.3607419842074115
1104	52-53	-1.420916889930485
1104	54-55	-1.3777670385255298
1104	56-57	-1.1209654499771666
1104	58-59	-1.3095677928831222
1104	60-61	-1.2452268381883798
1104	62-63	-1.5411887733777796
1104	64-65	-1.3740092576699716
1104	66-67	-1.338974820140944
1104	68-69	-1.1620840430572343
1104	70-71	-1.467252617733326
1104	72-73	-1.0440944227008835
1104	74-75	-1.2564018798799417
1104	76-77	-1.0950137184341813
1104	78-79	-1.0711095803840358
1104	80-81	-1.210268654813234
1104	82-83	-1.3191622347651943
1104	84-85	-0.9234547573602683
1104	86-87	-1.0722652430700172
1104	88-89	-0.8637874352886676
1104	90-91	-1.0285809209525247
1104	92-93	-1.141683457533329
1104	94-95	-1.1560094715223599
1104	96-97	-1.1625377024423464
1104	98-99	-0.938578684838653
1104	100	-1.0429156714425787
1105	1	1.2120406911174904
1105	2	1.403853711624464
1105	3	1.0206650958167245
1105	4	0.655484876451343
1105	5	0.6733320192421814
1105	6	0.7689581555023537
1105	7	1.5052972012695705
1105	8	1.156432421784281
1105	9	1.4245678443760426
1105	10-11	1.1668169735039058
1105	12-13	1.1324482607739625
1105	14-15	1.400276463779143
1105	16-17	1.3707335705487438
1105	18-19	1.4593254358091698
1105	20-21	1.3715095913227557
1105	22-23	1.0585354628522552
1105	24-25	1.1444467069422117
1105	26-27	1.0934583553478774
1105	28-29	0.8600377064260627
1105	30-31	1.2283952828175444
1105	32-33	1.5400094054616105
1105	34-35	1.812626432371239
1105	36-37	1.85766853934269
1105	38-39	2.1415644901718736
1105	40-41	2.310942186572319
1105	42-43	2.3187490555704997
1105	44-45	2.327629496720377
1105	46-47	2.3541937383682807
1105	48-49	2.268640672592916
1105	50-51	2.36194341782225
1105	52-53	2.3127755378603077
1105	54-55	2.0525374111231827
1105	56-57	2.443265619499808
1105	58-59	2.409230021324529
1105	60-61	1.9853274163003007
1105	62-63	2.172815910462969
1105	64-65	2.262196831322999
1105	66-67	2.562633298516353
1105	68-69	2.5595084627975666
1105	70-71	2.812060418956758
1105	72-73	2.5294106514599264
1105	74-75	2.537602803960805
1105	76-77	2.4686396773503603
1105	78-79	2.414589092527752
1105	80-81	2.4604105020954314
1105	82-83	2.381384213322235
1105	84-85	2.399386772694374
1105	86-87	2.3615520871407547
1105	88-89	2.394495156436548
1105	90-91	2.7036439034034494
1105	92-93	2.2498387907102284
1105	94-95	2.202273120202854
1105	96-97	2.3323412983382923
1105	98-99	2.348103594630512
1105	100	2.667435616613627
1106	1	0.34089633808832076
1106	2	0.634459545581084
1106	3	0.17046315116451893
1106	4	0.11394411354932643
1106	5	0.3289789900723932
1106	6	0.4956597261829785
1106	7	-0.3068194778628168
1106	8	0.2785864980744819
1106	9	0.5332589438524806
1106	10-11	0.39012288225483616
1106	12-13	0.6615731672810696
1106	14-15	0.06029142264227261
1106	16-17	0.15418906793094322
1106	18-19	0.13708160634021027
1106	20-21	-0.20201322094351326
1106	22-23	0.31027817040648387
1106	24-25	0.40975710335207793
1106	26-27	0.7995316537771942
1106	28-29	1.1311222240027305
1106	30-31	1.0762935625482868
1106	32-33	1.1054245139133698
1106	34-35	1.4223347345402786
1106	36-37	1.4246992050120966
1106	38-39	1.153396950904856
1106	40-41	1.0955495164152538
1106	42-43	1.142982413835263
1106	44-45	1.3520872379320465
1106	46-47	1.1980980016442722
1106	48-49	1.4610864467140843
1106	50-51	1.4142844799015322
1106	52-53	1.4120874301564932
1106	54-55	1.3902786227910973
1106	56-57	1.4484563450046686
1106	58-59	1.4768740003821215
1106	60-61	1.4283491066518366
1106	62-63	1.4957179299094925
1106	64-65	1.67396945660348
1106	66-67	1.6831119821364027
1106	68-69	1.5327919332538116
1106	70-71	1.742800882681518
1106	72-73	1.5403904569947073
1106	74-75	1.8878346663392662
1106	76-77	1.8964033273129672
1106	78-79	1.9184335203512362
1106	80-81	1.868952012940607
1106	82-83	1.8871433756259002
1106	84-85	2.10273755803469
1106	86-87	1.920310501501266
1106	88-89	1.9152730173191195
1106	90-91	1.6408465959988092
1106	92-93	2.1252464197304235
1106	94-95	2.374359881608985
1106	96-97	2.1850862497219907
1106	98-99	2.057916608841431
1106	100	1.7774430960451895
1107	1	-0.8611580666464853
1107	2	-1.2835065368227419
1107	3	-0.6992727923820325
1107	4	-0.5020927633002081
1107	5	-0.5732518316894968
1107	6	-0.6321288010193875
1107	7	-0.8496717428298126
1107	8	-1.1108967707623059
1107	9	-0.286084329537001
1107	10-11	-0.8142078712165883
1107	12-13	-0.8255796895365961
1107	14-15	-0.6637297474009785
1107	16-17	-0.744157733799085
1107	18-19	-0.6782211480417644
1107	20-21	-0.5727450670623355
1107	22-23	-0.6849272700669999
1107	24-25	-0.8650408706975412
1107	26-27	-1.3921938185651754
1107	28-29	-1.0479126041329394
1107	30-31	-1.4867755246358705
1107	32-33	-1.1668694765259673
1107	34-35	-0.9293611452685084
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2216	86-87	-2.292679718789163
2216	88-89	-2.439898374560755
2216	90-91	-2.53538574619224
2216	92-93	-2.924393015219689
2216	94-95	-2.6132524862661484
2216	96-97	-2.4071196181037564
2216	98-99	-2.5404139255851206
2216	100	-2.1426991542758635
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7127.0
3	915.0
4	624.0
5	791.0
6	1992.0
7	4287.0
8	4384.0
9	2452.0
10	1353.0
11	1294.0
12	1191.0
13	796.0
14	633.0
15	565.0
16	492.0
17	466.0
18	410.0
19	380.0
20	361.0
21	336.0
22	463.0
23	981.0
24	1666.0
25	1160.0
26	1277.0
27	978.0
28	1078.0
29	1137.0
30	1363.0
31	1652.0
32	2159.0
33	2920.0
34	4323.0
35	8189.0
36	11627.0
37	20670.0
38	27425.0
39	9567.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10030524322862	23.559367876048064	12.220547892276187	20.119778988447123
2	15.859905583842629	30.384695619924745	32.10381141492888	21.651587381303745
3	18.82070223156333	35.941460097669534	25.12904123137788	20.108796439389256
4	12.084795276442494	26.559191307170348	29.33907290909934	32.01694050728781
5	11.788030329965913	46.55464263354383	27.002481121064797	14.654845915425463
6	26.457562008703245	42.35992487072664	15.066047288157863	16.116465832412253
7	21.886424944155454	44.44916793556815	17.18698068435657	16.47742643591983
8	22.115882648525375	43.238375014495	16.218159328978395	18.427583008001235
9	22.111333575553303	29.306812823228377	15.811810542753113	32.770043058465205
10-11	20.37130090602678	38.64374284919138	20.871857509508644	20.113098735273198
12-13	21.079323847405675	38.70212099776188	21.216548962307545	19.002006192524902
14-15	20.542809436937635	38.87480288179091	19.813858569617512	20.768529111653937
16-17	19.261949843502453	40.471424707291625	21.050658835349125	19.215966613856793
18-19	18.80923300505558	38.79616889040058	23.525842210231755	18.868755894312088
20-21	21.336945725228897	37.600052562621016	22.567915930725558	18.495085781424525
22-23	20.876815429849742	37.42596291674233	22.21672726148758	19.48049439192035
24-25	20.640646362281114	38.02090172481713	21.929045364312582	19.40940654858917
26-27	20.4795227982221	37.203634717821956	22.822592811800067	19.49424967215588
28-29	20.208959606809735	37.74487453923015	22.90943795738893	19.136727896571177
30-31	19.937249325739366	39.03292864815573	22.51760032766362	18.51222169844128
32-33	19.10994157470092	38.70714705245912	23.168413243067793	19.01449812977217
34-35	18.818140740197194	38.73541599885684	22.79294483858634	19.653498422359625
36-37	19.139072080112193	38.81361629133393	22.465876207806456	19.58143542074742
38-39	19.004706737048743	38.377296369861426	22.968335793614603	19.649661099475228
40-41	18.637059250900435	38.06595971618927	23.490902907668765	19.806078125241534
42-43	18.431374064384993	38.02934459613505	23.854753136749526	19.68452820273043
44-45	18.115706822467466	37.94437231444558	24.097344585613158	19.8425762774738
46-47	17.70645747188623	37.62414499362368	23.72183792557097	20.947559608919118
48-49	17.98231716270838	36.235518699136726	23.471493380528784	22.31067075762611
50-51	18.36680281151345	35.380014142577274	23.492134640426904	22.761048405482374
52-53	18.380548653484247	34.438265856193894	23.680824178159742	23.500361312162116
54-55	18.767097881021932	34.30433841826247	23.121358247940528	23.807205452775072
56-57	18.79313142865975	33.76867257594609	23.185678626903965	24.2525173684902
58-59	18.66277182732879	33.08217801888688	22.997326161883123	25.25772399190121
60-61	18.849976044387432	32.43628579818555	22.538753999041774	26.17498415838524
62-63	18.79095826893354	31.991499227202475	22.331143740340032	26.886398763523957
64-65	18.59005815189042	31.572419389115357	22.972508259114004	26.865014199880218
66-67	18.532629065350864	31.668102765423896	22.886906256882646	26.912361912342593
68-69	19.22173365377928	31.643779510521398	22.76145066188574	26.373036173813592
70-71	18.88979471982134	30.98791810429763	23.38215804989626	26.74012912598477
72-73	18.923022830803234	31.011486880691763	23.260064060706988	26.80542622779802
74-75	19.696073999374065	30.436260929706005	24.08959364483237	25.77807142608756
76-77	19.908897380123555	30.719896147649607	23.945925688388858	25.425280783837977
78-79	20.21819923815705	30.043346236758534	24.65365506903719	25.084799456047225
80-81	21.35357109744414	29.488209660969805	24.667768952622307	24.490450288963746
82-83	21.19759229782565	29.09950702375249	24.85666599700196	24.846234681419897
84-85	21.582731033843373	29.32139421869749	24.902170595938486	24.193704151520652
86-87	21.58264766098814	29.71916405918028	24.545138486499017	24.15304979333256
88-89	21.65483300240279	28.923072168612336	24.792749916945446	24.629344912039432
90-91	21.9456891223733	28.72334672435105	24.87986711990111	24.451097033374538
92-93	21.685266951755146	29.791360042029304	24.011171760015763	24.512201246199787
94-95	21.156401619333106	29.858385611421863	24.29501838746562	24.690194381779413
96-97	20.555978351148696	30.44823629852545	24.600651319830487	24.395134030495367
98-99	20.13806530867679	32.126646141008955	23.67584397924771	24.05944457106655
100	19.8199660021635	33.53036624942049	23.772214495441197	22.87745325297481
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3279.0
1	2064.5
2	792.0
3	666.5
4	485.0
5	324.5
6	291.0
7	341.5
8	421.5
9	443.0
10	331.5
11	223.5
12	189.0
13	165.5
14	170.5
15	172.0
16	166.0
17	173.0
18	185.0
19	206.5
20	260.0
21	341.0
22	430.0
23	511.0
24	551.0
25	641.0
26	873.0
27	1177.5
28	1505.0
29	1724.5
30	1857.5
31	2080.0
32	2249.5
33	2378.0
34	2676.5
35	2972.5
36	3461.0
37	3706.5
38	3492.0
39	3532.5
40	3908.0
41	4042.0
42	3597.5
43	3356.5
44	3626.0
45	3859.5
46	3758.5
47	3497.5
48	3541.0
49	3757.5
50	3942.5
51	3917.5
52	3610.0
53	3339.5
54	3414.0
55	3547.5
56	3142.0
57	2901.0
58	2930.5
59	2508.0
60	2222.0
61	2126.5
62	1757.0
63	1424.5
64	1054.5
65	887.5
66	851.5
67	759.5
68	654.5
69	420.5
70	314.5
71	209.0
72	101.0
73	89.5
74	79.5
75	85.0
76	77.0
77	54.0
78	40.0
79	54.5
80	58.0
81	39.0
82	28.0
83	16.0
84	4.0
85	1.0
86	1.5
87	2.5
88	1.5
89	1.5
90	1.5
91	1.0
92	0.5
93	0.5
94	1.5
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06564213452776275
2	0.04865240559116534
3	0.05714727005946405
4	0.07413699899606148
5	0.08726542590161403
6	0.08726542590161403
7	0.08726542590161403
8	0.10425515483821145
9	0.10116611321337554
10-11	0.10348289443200248
12-13	0.1085025870723608
14-15	0.09730481118233068
16-17	0.0733647385898525
18-19	0.09884933199474863
20-21	0.09305737894818132
22-23	0.060622441887404434
24-25	0.02085103096764229
26-27	0.18263958606842226
28-29	0.0671866553401807
30-31	0.06873117615259866
32-33	0.07259247818364353
34-35	0.018920379952119856
36-37	0.055216619043941616
38-39	0.07799830102710635
40-41	0.08340412387056916
42-43	0.07452312919916596
44-45	0.07259247818364353
46-47	0.08108734265194223
48-49	0.07645378021468839
50-51	0.07220634798053904
52-53	0.07761217082400186
54-55	0.06795891574638968
56-57	0.06873117615259866
58-59	0.06795891574638968
60-61	0.06486987412155379
62-63	0.06950343655880763
64-65	0.06757278554328519
66-67	0.06834504594949417
68-69	0.0671866553401807
70-71	0.06293922310603135
72-73	0.06293922310603135
74-75	0.0644837439184493
76-77	0.05830566066877751
78-79	0.05174144721600124
80-81	0.04788014518495637
82-83	0.055602749247046106
84-85	0.042860452544598034
86-87	0.04247432234149355
88-89	0.0440188431539115
90-91	0.038613020310448685
92-93	0.043632712950807014
94-95	0.04170206193528458
96-97	0.046721754575642906
98-99	0.04517723376322496
100	0.05405822843462816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	129490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.82083558575952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72421062421527	41.712101320565296
2	6.586269434067553	6.5626689319638585
3	2.2956613395749694	3.43115298478647
4	1.4601708182846869	2.909877210595413
5	0.9300451071876986	2.316781218626921
6	0.716134732534528	2.140705846011275
7	0.5332258614542805	1.8596030581512084
8	0.4324709748422799	1.7236852266584293
9	0.37201804287507945	1.6680824774113832
>10	2.8071861485282037	26.117846937987487
>50	0.11625563839846233	3.793343115298479
>100	0.02480120285833863	2.0966870028573634
>500	0.0	0.0
>1k	0.0015500751786461644	3.6674646690864163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4749	3.6674646690864163	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.2517568924241254	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	313	0.24171750714340876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	304	0.234767163487528	No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	235	0.18148119545910882	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.1698972893659742	No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.11197775890030119	No Hit
CTCCTACACCGGTTAACTTCAATGGTGCGTCTATTTTTGGGAGCTGCCAT	134	0.10348289443200248	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134	0.10348289443200248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.722604062089737E-4	0.0
8	0.0	0.0	0.0	7.722604062089737E-4	0.0
9	0.0	0.0	0.0	7.722604062089737E-4	0.0
10-11	0.0	0.0	0.0	7.722604062089737E-4	0.0
12-13	0.0	0.0	0.0	7.722604062089737E-4	0.0
14-15	0.0	0.0	0.0	7.722604062089737E-4	0.0
16-17	0.0	0.0	0.0	0.0011583906093134604	0.0
18-19	0.0	0.0	0.0	0.0023167812186269208	0.0
20-21	0.0	0.0	0.0	0.0027029114217314077	0.0
22-23	0.0	0.0	0.0	0.007722604062089736	0.0
24-25	0.0	0.0	0.0	0.01930651015522434	0.0
26-27	0.0	0.0	0.0	0.04749401498185188	0.0
28-29	0.0	0.0	0.0	0.0876515561047185	0.0
30-31	0.0	0.0	0.0	0.1876592787087806	0.0
32-33	0.0	0.0	0.0	0.3285968028419183	0.0
34-35	0.0	0.0	0.0	0.5529384508456251	0.0
36-37	0.0	0.0	0.0	0.9016140242489767	0.0
38-39	0.0	0.0	0.0	1.2997142636497028	0.0
40-41	0.0	0.0	0.0	1.7109429299559813	0.0
42-43	0.0	0.0	0.0	2.0322032589389143	0.0
44-45	0.0	0.0	0.0	2.3260483435014288	0.0
46-47	0.0	0.0	0.0	2.6797436095451386	0.0
48-49	0.0	0.0	0.0	3.0442505212757744	0.0
50-51	0.0	0.0	0.0	3.354313074368677	0.0
52-53	0.0	0.0	0.0	3.662058846242953	0.0
54-55	0.0	0.0	0.0	4.003397945787319	0.0
56-57	0.0	0.0	0.0	4.377558112595567	0.0
58-59	0.0	0.0	0.0	4.743609545138621	0.0
60-61	0.0	0.0	0.0	5.077226040620897	0.0
62-63	0.0	0.0	0.0	5.440188431539115	0.0
64-65	0.0	0.0	0.0	5.8475557958143485	0.0
66-67	0.0	0.0	0.0	6.292377789790717	0.0
68-69	0.0	0.0	0.0	6.801683527685535	0.0
70-71	0.0	0.0	0.0	7.3461271140628615	0.0
72-73	0.0	0.0	0.0	7.88902617962777	0.0
74-75	0.0	0.0	0.0	8.42343038072438	0.0
76-77	0.0	0.0	0.0	9.0103482894432	0.0
78-79	0.0	0.0	0.0	9.655957989033903	0.0
80-81	0.0	0.0	0.0	10.3270522820295	0.0
82-83	0.0	0.0	0.0	10.996215924009576	0.0
84-85	0.0	0.0	0.0	11.70206193528458	0.0
86-87	0.0	0.0	0.0	12.455015831338327	0.0
88	0.0	0.0	0.0	13.019538188277087	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTCT	20	0.0021556313	70.52467	4
TACAAGA	30	1.3642172E-4	62.6886	2
CCTAGTC	25	0.0052183275	56.419735	3
TCCTAGT	25	0.0052183275	56.419735	2
TAACAAC	160	0.0	47.034653	1
GAGAACA	290	0.0	45.412766	1
GTACAAA	105	2.1827873E-11	44.794907	1
GTACATA	75	1.4588841E-7	43.899006	1
CGTATCA	60	8.802644E-5	39.15007	94
CTGTCCT	50	0.0016903108	37.627716	9
TACATAA	50	0.0016935299	37.61316	2
TACACTG	50	0.0016935299	37.61316	5
ATCTCTA	50	0.0016967539	37.598606	7
GGAAACA	195	0.0	36.1805	1
AGAACAA	305	0.0	35.455025	2
CTCTTAC	55	0.002698362	34.207016	1
GAAAACA	365	0.0	32.215515	1
AATCAAT	30	0.0041637025	31.320055	68-69
ACGCATC	30	0.0041637025	31.320055	54-55
CGCATCC	30	0.0041637025	31.320055	56-57
>>END_MODULE