FastQCFastQC Report
Wed 25 May 2016
SRR1033063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129490
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29342.265812031817129No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29082.2457332612556953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16581.2804077534944784No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12570.97073133060468No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.6880840219321955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.6216696269982238No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.617808324967179No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.5035137848482508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.4888408371302803No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT5890.45486137925708553TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4400.3397945787319484RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2530.19538188277087032No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.18302571627152675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2330.17993667464669086No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.17221407058460111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1940.1498185188045409No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT1860.1436404355548691TruSeq Adapter, Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1800.13900687311761525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1720.13282878986794347No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.1258784462120627No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT1580.12201714418101785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1470.11352227971271912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1430.11043323808788323No Hit
GTACAGAGAAGCACCTATTGATAAAAAGGGGAATTTCAATTACATCGAGT1380.10657193605683836No Hit
GTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAATGATACCAGTGGAAG1330.1027106340257935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.060.088871
ATGTGAG250.00522702556.396065
CTAATAC250.00522702556.396063
CACAACC352.928773E-453.71053794
TAATACA450.001010968641.7748574
GTATCAA21900.035.9626921
GTACACG902.516874E-531.4404371
TACACGG902.5235557E-531.4282552
TATTTGT600.004148898631.3311466
TATCAAC26350.029.877722
GAGAGAC953.7286736E-529.6821379
ACGGGAG953.7286736E-529.6821375
ATCAACG26750.029.5156943
AGTGAGC804.759699E-429.3729594
TCAACGC27200.029.0273844
CAACGCA27300.028.9210595
AGACTCA650.00613638528.9210558
TTAGGAC650.00613638528.9210553
GCGACCA505.310368E-528.1980364-65
AACGCAG28000.028.0301866
Produced by FastQC (version 0.11.5)