##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033063_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129490
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.404448219939766	33.0	31.0	34.0	30.0	34.0
2	32.34387211367673	33.0	31.0	34.0	30.0	34.0
3	32.37182021777743	34.0	31.0	34.0	30.0	34.0
4	36.04248976754962	37.0	35.0	37.0	35.0	37.0
5	36.060892733029576	37.0	35.0	37.0	35.0	37.0
6	36.19284886863851	37.0	36.0	37.0	35.0	37.0
7	36.12138389064793	37.0	36.0	37.0	35.0	37.0
8	36.166252220248666	37.0	37.0	37.0	35.0	37.0
9	37.890423970963006	39.0	38.0	39.0	35.0	39.0
10-11	37.85632867402888	39.0	38.0	39.0	35.0	39.0
12-13	37.79053208741988	39.0	38.0	39.0	35.0	39.0
14-15	39.00501197003629	40.0	38.0	41.0	35.5	41.0
16-17	38.996362653486756	40.0	38.5	41.0	35.5	41.0
18-19	39.16495096146421	41.0	39.0	41.0	36.0	41.0
20-21	39.19321183102942	41.0	39.0	41.0	35.5	41.0
22-23	39.002984786469995	41.0	39.0	41.0	35.0	41.0
24-25	38.83801837979767	41.0	38.0	41.0	35.0	41.0
26-27	38.523959379102635	40.0	38.0	41.0	35.0	41.0
28-29	38.29947100162175	40.0	38.0	41.0	35.0	41.0
30-31	37.80070661827168	40.0	36.5	41.0	34.0	41.0
32-33	37.15734805776508	40.0	35.0	41.0	33.0	41.0
34-35	36.69975287667001	40.0	35.0	41.0	33.0	41.0
36-37	36.26538342729168	39.5	35.0	41.0	31.5	41.0
38-39	35.80705459881072	39.0	35.0	41.0	30.5	41.0
40-41	35.4510077998301	39.0	35.0	41.0	29.0	41.0
42-43	34.93594872190903	39.0	35.0	41.0	24.5	41.0
44-45	34.16570777666229	39.0	35.0	41.0	10.0	41.0
46-47	33.03265889257858	39.0	34.5	41.0	2.0	41.0
48-49	31.985728627693256	38.0	33.0	41.0	2.0	41.0
50-51	31.412267356552633	38.0	33.0	41.0	2.0	41.0
52-53	31.156815198084793	38.0	32.0	41.0	2.0	41.0
54-55	30.927453857440728	37.0	31.5	41.0	2.0	41.0
56-57	30.757749633176307	37.0	31.0	41.0	2.0	41.0
58-59	30.530021623291375	36.5	31.0	40.5	2.0	41.0
60-61	30.270155996602053	36.0	31.0	40.0	2.0	41.0
62-63	29.954278322650396	35.5	30.0	40.0	2.0	41.0
64-65	29.56655340180709	35.0	29.5	39.5	2.0	41.0
66-67	29.128145030504285	35.0	28.5	39.0	2.0	41.0
68-69	28.64785697737277	35.0	26.5	39.0	2.0	41.0
70-71	28.112792493628852	35.0	25.0	37.5	2.0	40.5
72-73	27.693922310603135	35.0	22.5	37.0	2.0	40.0
74-75	27.390736736427524	35.0	21.0	36.5	2.0	39.0
76-77	27.083983319175225	35.0	19.5	36.0	2.0	39.0
78-79	26.77982083558576	35.0	18.0	36.0	2.0	39.0
80-81	26.50237083944706	35.0	15.0	35.0	2.0	37.0
82-83	26.267854660591553	35.0	9.0	35.0	2.0	37.0
84-85	26.086736427523363	35.0	6.0	35.0	2.0	36.5
86-87	25.921928334234302	35.0	2.0	35.0	2.0	36.0
88-89	25.767711792416403	35.0	2.0	35.0	2.0	36.0
90-91	25.608672484361726	35.0	2.0	35.0	2.0	35.5
92-93	25.454857517955055	34.0	2.0	35.0	2.0	35.0
94-95	25.2895899297243	34.0	2.0	35.0	2.0	35.0
96-97	25.14582979380647	34.0	2.0	35.0	2.0	35.0
98-99	25.01984709243957	34.0	2.0	35.0	2.0	35.0
100	24.901359178314927	34.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07013944917122927
1101	2	0.23663970089990727
1101	3	0.1822761259771042
1101	4	0.19051968908775763
1101	5	0.10724561155875989
1101	6	-0.04294274027795808
1101	7	-0.08380014944003022
1101	8	0.10925175651843233
1101	9	0.2565103212494009
1101	10-11	0.17498120986277144
1101	12-13	0.24471211317013086
1101	14-15	0.5056428004202758
1101	16-17	0.19578728597133477
1101	18-19	0.15954476529478967
1101	20-21	0.002065219761284709
1101	22-23	0.2483534755392114
1101	24-25	0.07953039565962428
1101	26-27	0.19222860688777388
1101	28-29	0.2528803141321845
1101	30-31	-0.0037003537696804756
1101	32-33	-0.12204689576294925
1101	34-35	-0.19493741168624723
1101	36-37	-0.7581827988078871
1101	38-39	-1.029870392224197
1101	40-41	-0.15624360579212748
1101	42-43	-0.17610334809755557
1101	44-45	-0.5636530036534637
1101	46-47	-0.7244698284879973
1101	48-49	-0.7863864737194639
1101	50-51	-0.6739307460349089
1101	52-53	-0.5499914663161647
1101	54-55	-0.29671137310347717
1101	56-57	-0.5446316757467109
1101	58-59	-0.7004620812099418
1101	60-61	-0.6439338947719335
1101	62-63	-0.46459451471781676
1101	64-65	-0.2980235435748675
1101	66-67	-0.08973674742924587
1101	68-69	-0.16469224531622828
1101	70-71	-0.27398602103315994
1101	72-73	-0.04487730197058326
1101	74-75	0.0497297053762189
1101	76-77	-0.046497743146364456
1101	78-79	0.20170650966420212
1101	80-81	-0.09883908733789681
1101	82-83	0.16988023150774012
1101	84-85	0.18566134258738032
1101	86-87	-0.08297690908688082
1101	88-89	-0.008782287786736731
1101	90-91	-0.016606020836825053
1101	92-93	-0.09887515463482899
1101	94-95	-0.14086552395663787
1101	96-97	-0.28967828056712364
1101	98-99	-0.40764021498678815
1101	100	-0.29970791328641155
1102	1	0.44725543336654994
1102	2	0.2647333714071678
1102	3	0.4739864357182384
1102	4	0.3759154694259266
1102	5	0.35365441000257647
1102	6	0.2710255374416235
1102	7	0.2969420322149148
1102	8	0.11707010157304865
1102	9	0.3065626929387619
1102	10-11	0.2641066026504362
1102	12-13	0.2786975987305027
1102	14-15	0.4935411993429142
1102	16-17	0.7062055113192329
1102	18-19	0.5387719087932155
1102	20-21	0.41110681784598313
1102	22-23	0.49532339922618007
1102	24-25	0.5056489053109843
1102	26-27	0.6752078078454247
1102	28-29	0.2993975967896745
1102	30-31	0.09662883970630531
1102	32-33	0.1422056854131739
1102	34-35	0.22274551514302487
1102	36-37	0.42951359502721687
1102	38-39	0.6259914961492825
1102	40-41	0.8494611675132759
1102	42-43	0.7800914745868681
1102	44-45	0.8427326016136334
1102	46-47	-0.19080231389873603
1102	48-49	-0.33002331346209246
1102	50-51	-0.6253560041525219
1102	52-53	-0.8673264954946767
1102	54-55	-0.6569163708589798
1102	56-57	-0.9381114004212563
1102	58-59	-0.5626871298407963
1102	60-61	-0.5719041177256976
1102	62-63	-0.13882390271722045
1102	64-65	-0.5134769327713364
1102	66-67	-0.199268394892961
1102	68-69	-0.2833889384181276
1102	70-71	-0.3781495703373636
1102	72-73	-0.33409995646706747
1102	74-75	-0.44320011656003544
1102	76-77	-0.5265403886648414
1102	78-79	-0.2724630249759308
1102	80-81	-0.23003016918736918
1102	82-83	-0.4549514309193121
1102	84-85	-0.5535089402197428
1102	86-87	-0.66239483516798
1102	88-89	-0.6653362306267816
1102	90-91	-0.45918420428736795
1102	92-93	-0.28208254652469833
1102	94-95	-0.10173639033401116
1102	96-97	-0.24026185082000495
1102	98-99	-0.5775302344391307
1102	100	-0.5768663750550793
1103	1	0.13519237158883612
1103	2	0.20388303612395475
1103	3	0.028844475381532675
1103	4	-0.04643436649931232
1103	5	0.11703006986846276
1103	6	-0.06279526438459726
1103	7	0.18080563615270506
1103	8	0.34067546610436494
1103	9	0.24913652658122487
1103	10-11	0.06033288314694829
1103	12-13	0.021938431944946046
1103	14-15	0.10611075277421378
1103	16-17	0.5673839961244838
1103	18-19	0.44145060760902766
1103	20-21	0.2846800807936134
1103	22-23	0.14923265874964642
1103	24-25	0.1367482062098233
1103	26-27	-0.024660218977231807
1103	28-29	0.0833539386358737
1103	30-31	-0.2352658670992298
1103	32-33	-0.27079902281909796
1103	34-35	0.31286179421279314
1103	36-37	0.15021198281434778
1103	38-39	-0.14756749942782932
1103	40-41	0.03015741519482873
1103	42-43	0.28390799620193263
1103	44-45	0.3611910122285522
1103	46-47	1.1888880841609009
1103	48-49	1.5008370089804828
1103	50-51	1.1961770248102468
1103	52-53	1.3907345688511654
1103	54-55	1.416703261042862
1103	56-57	1.4937262369021873
1103	58-59	1.321872008410022
1103	60-61	1.1559101211100824
1103	62-63	1.0539817756962506
1103	64-65	1.2889021512634748
1103	66-67	1.6214799286909987
1103	68-69	1.6857757070377133
1103	70-71	1.8529283295056942
1103	72-73	2.0984153809562436
1103	74-75	1.8362441693580678
1103	76-77	2.0754534763658334
1103	78-79	2.0727360048428736
1103	80-81	1.7911212076138874
1103	82-83	1.9999993466523804
1103	84-85	2.0977430215437067
1103	86-87	2.0365636467837938
1103	88-89	1.7711383021168317
1103	90-91	2.202126253695223
1103	92-93	2.1243948396930215
1103	94-95	2.2950391839276527
1103	96-97	2.164164600884945
1103	98-99	2.252456211558872
1103	100	2.225958564308595
1104	1	0.2611613638368979
1104	2	-0.13497831855897147
1104	3	-0.08781583723760633
1104	4	-0.20385589401233517
1104	5	0.01911419745773202
1104	6	0.11902580563700838
1104	7	0.1994865358858391
1104	8	0.16819484594932987
1104	9	0.2892305664846404
1104	10-11	0.17486458984411257
1104	12-13	0.2815806990691101
1104	14-15	0.19405077056554632
1104	16-17	0.22712475098533247
1104	18-19	0.3728427794224771
1104	20-21	0.1588858248525824
1104	22-23	0.13706465773299925
1104	24-25	0.2806832678440898
1104	26-27	-0.030648908772633376
1104	28-29	0.09406050872101446
1104	30-31	-0.10052828926214374
1104	32-33	-0.20068210834458
1104	34-35	-0.1706971078077899
1104	36-37	-1.1122578354598929
1104	38-39	-1.000931967594049
1104	40-41	-0.9631708349004882
1104	42-43	-1.0932364948388624
1104	44-45	-1.014889175851252
1104	46-47	-1.7915096792143714
1104	48-49	-2.572488699495821
1104	50-51	-2.7336901755964185
1104	52-53	-2.870829797650295
1104	54-55	-2.7800180504325454
1104	56-57	-2.7216442040687134
1104	58-59	-2.589473143451709
1104	60-61	-2.6060990287183543
1104	62-63	-2.4477179320175644
1104	64-65	-2.578028208375617
1104	66-67	-2.233345970406724
1104	68-69	-2.128606679542539
1104	70-71	-2.4018848614969777
1104	72-73	-2.7922950015574166
1104	74-75	-2.4852229802162107
1104	76-77	-2.90337166024619
1104	78-79	-2.781883387710181
1104	80-81	-2.695567062814373
1104	82-83	-2.4907555141939817
1104	84-85	-2.324118078977328
1104	86-87	-2.102335832184309
1104	88-89	-2.495954085734226
1104	90-91	-2.7670725009114605
1104	92-93	-2.3344309323237518
1104	94-95	-2.4424115722040014
1104	96-97	-2.4647749085825836
1104	98-99	-2.259282891249619
1104	100	-2.4780699018243304
1105	1	0.028780411455947785
1105	2	0.07451426535983785
1105	3	-7.900761134891354E-4
1105	4	-0.20042107746276372
1105	5	-0.16024567763984265
1105	6	-0.17977200857064446
1105	7	-0.10477575919613002
1105	8	0.04629008404456414
1105	9	0.18368802159783826
1105	10-11	0.23325647118681303
1105	12-13	0.14793510968581103
1105	14-15	0.07820377603002981
1105	16-17	-0.09381201716455223
1105	18-19	-0.12305105352053403
1105	20-21	0.16257044624212114
1105	22-23	0.07797020027788903
1105	24-25	0.17935617963175332
1105	26-27	0.3294759936473497
1105	28-29	0.5019918123900311
1105	30-31	0.2169268239306703
1105	32-33	0.39049665824402524
1105	34-35	0.4770312294287393
1105	36-37	0.8130192917844496
1105	38-39	0.8274989457548969
1105	40-41	0.799592631141671
1105	42-43	0.6441405543414689
1105	44-45	0.5583504806670945
1105	46-47	0.9362967220346547
1105	48-49	1.009275547186462
1105	50-51	1.1998148985643908
1105	52-53	1.338475432638539
1105	54-55	1.3881318324714371
1105	56-57	1.2954205890616528
1105	58-59	1.4822208456193273
1105	60-61	1.5752124466914807
1105	62-63	1.9205100148989054
1105	64-65	1.8551646097352368
1105	66-67	1.3639217891561195
1105	68-69	1.3228687302935285
1105	70-71	1.5389582298379167
1105	72-73	1.698415380956245
1105	74-75	2.0172906809859725
1105	76-77	1.8375963335086887
1105	78-79	1.8056263702913782
1105	80-81	2.0653072541255177
1105	82-83	1.9002983499746406
1105	84-85	2.1218460115769275
1105	86-87	2.170716470704061
1105	88-89	1.9380818237115207
1105	90-91	1.8836378816021977
1105	92-93	1.9696440091282383
1105	94-95	1.6296571241269895
1105	96-97	1.918300813509532
1105	98-99	1.7530043843163483
1105	100	1.734264212149128
1106	1	0.14423291706552277
1106	2	-0.0023144556573626573
1106	3	0.2146474706329613
1106	4	0.2484944049605744
1106	5	0.15203554898693028
1106	6	0.15719134221469488
1106	7	0.13794675389287647
1106	8	0.16470444455316624
1106	9	0.08226692960082715
1106	10-11	0.19728040536781322
1106	12-13	0.21554917101714466
1106	14-15	0.37087393309809613
1106	16-17	0.356255297719521
1106	18-19	0.35075597714513407
1106	20-21	0.2497507005427977
1106	22-23	0.4398998936211882
1106	24-25	0.5070300764155959
1106	26-27	0.7702837722561782
1106	28-29	0.9061653352022958
1106	30-31	0.8860966070271559
1106	32-33	0.7079237337862807
1106	34-35	0.46620101252522517
1106	36-37	0.40908511078220755
1106	38-39	0.7060030594422528
1106	40-41	0.8667579265792185
1106	42-43	1.1238264182158133
1106	44-45	0.9965404582287931
1106	46-47	1.1070970212119136
1106	48-49	1.0070466765806287
1106	50-51	0.879590515617501
1106	52-53	1.083995253132187
1106	54-55	1.079006925964329
1106	56-57	1.00617601164954
1106	58-59	1.2505978089701095
1106	60-61	0.8272543315082359
1106	62-63	1.065685781540644
1106	64-65	0.9910359635123491
1106	66-67	0.6880055587896265
1106	68-69	1.025800667466303
1106	70-71	0.9716882223584875
1106	72-73	0.9413473181290186
1106	74-75	1.1669241888393813
1106	76-77	0.9151131622297051
1106	78-79	0.37353960888524895
1106	80-81	0.578500971403006
1106	82-83	0.5855040343426303
1106	84-85	0.5499985919808168
1106	86-87	0.4040298588865596
1106	88-89	0.2320234841453228
1106	90-91	0.01892583971738304
1106	92-93	0.015238623937513296
1106	94-95	0.30045742330425185
1106	96-97	0.3962813669874663
1106	98-99	0.2634905473679545
1106	100	0.0813098366816618
1107	1	-0.12833138978628966
1107	2	0.05411053865176285
1107	3	0.00849563817222787
1107	4	-0.11051424516463015
1107	5	0.08391581304338303
1107	6	0.21501060012500517
1107	7	-0.10673228093526177
1107	8	-0.07818196564487323
1107	9	0.2718867793618074
1107	10-11	-0.01821868409269456
1107	12-13	-0.1400307288235041
1107	14-15	0.08961681950829359
1107	16-17	0.29381220643793426
1107	18-19	0.1553029837465445
1107	20-21	0.09458907978250153
1107	22-23	0.31290808847664664
1107	24-25	0.08960462683672432
1107	26-27	0.137814502964126
1107	28-29	0.15425889313537056
1107	30-31	0.04753241399278352
1107	32-33	-0.0675002361659196
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2216	70-71	-2.6764595599194934
2216	72-73	-2.7166789586663995
2216	74-75	-2.785728186938556
2216	76-77	-2.735395580237938
2216	78-79	-2.604885759088674
2216	80-81	-2.6863908590820316
2216	82-83	-2.641049358920238
2216	84-85	-2.7333561447573054
2216	86-87	-2.700119108810764
2216	88-89	-3.1640745105202868
2216	90-91	-2.985796080661949
2216	92-93	-3.0341834841332194
2216	94-95	-2.656650153501044
2216	96-97	-2.499273310479687
2216	98-99	-2.6042462895381
2216	100	-2.602123928012599
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	2.0
5	26.0
6	83.0
7	182.0
8	258.0
9	323.0
10	472.0
11	776.0
12	1212.0
13	1622.0
14	1810.0
15	2373.0
16	3180.0
17	4370.0
18	5007.0
19	2159.0
20	970.0
21	859.0
22	912.0
23	1098.0
24	1326.0
25	1568.0
26	1695.0
27	1285.0
28	1153.0
29	1335.0
30	1494.0
31	1919.0
32	2323.0
33	3033.0
34	4256.0
35	8002.0
36	11971.0
37	20851.0
38	29026.0
39	10542.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.582454513848646	19.357206319774402	14.465175570749796	19.59516359562715
2	17.06867723557097	21.927638097597228	38.888716868962106	22.114967797869706
3	18.477245171404522	29.508298002146898	33.05222837461097	18.962228451837607
4	12.085476422161468	20.40838391795252	34.38595678297268	33.12018287691333
5	10.53440420109661	38.19986099312688	34.231214765618965	17.034520040157542
6	26.727160398486372	38.12031817128736	16.212834967951192	18.93968646227508
7	20.44559425438258	38.347362730712796	22.224109969881845	18.982933045022783
8	25.234381033284425	32.71372306741833	20.621669626998223	21.43022627229902
9	25.473009498802995	15.173372461193916	21.822534558653178	37.53108348134992
10-11	23.535203762481753	27.080292534500998	26.455142907891787	22.929360795125454
12-13	26.859989188354312	25.946405127809097	28.832728396015135	18.360877287821452
14-15	23.157772800988493	24.920457178160476	31.02980925167967	20.891960769171362
16-17	17.8743532319098	27.98787551162252	31.99320410842536	22.14456714804232
18-19	16.92614816702577	21.72501139092896	41.49155526724278	19.857285174802495
20-21	18.607228357402118	20.837130280330527	42.54923160089582	18.006409761371533
22-23	17.90292686693953	21.572322187041472	42.946945710093445	17.577805235925553
24-25	18.664133632972685	22.009977681501905	42.54840179474704	16.777486890778366
26-27	18.3736195845239	21.395474554019618	42.97204417329524	17.258861688161247
28-29	18.039230828635418	20.385357942698278	43.136535639817744	18.43887558884856
30-31	18.852421036373464	21.365742528380572	43.123793343115295	16.65804309213067
32-33	18.41802455788092	20.69155919376014	43.85357942698278	17.036836821376166
34-35	17.191288902617963	20.681905938682522	43.99915051355317	18.127654645146343
36-37	18.24001853424975	21.47617576646845	42.18202177774346	18.101783921538342
38-39	17.904085257548846	21.153370916673104	42.26002007877056	18.68252374700749
40-41	17.913738512626455	20.584987257703297	42.238010657193605	19.26326357247664
42-43	18.932736118619196	20.534018070893506	41.57193605683837	18.96130975364893
44-45	18.31685844466754	21.493551625608156	40.9464051278091	19.243184801915206
46-47	19.00687311761526	21.356861533709168	38.53579426982778	21.10047107884779
48-49	19.751718279403814	21.402424897675495	36.81210904316936	22.03374777975133
50-51	19.6470769943625	21.8762066568847	36.40088037686308	22.07583597188972
52-53	19.40883465904703	22.32990964553247	36.39199938219168	21.86925631322882
54-55	19.54668314155533	22.969341261873506	34.92200169897289	22.56197389759827
56-57	19.320796972739206	23.431152984786472	33.931577728009884	23.316472314464438
58-59	18.630396169588384	23.944706154915437	34.45401189281026	22.970885782685922
60-61	19.211135995057532	24.476407444590315	32.83458182099004	23.47787473936211
62-63	18.51030967642289	25.244034288362037	32.66970422426442	23.575951810950652
64-65	18.465132442659666	24.89960614719283	33.33191752258862	23.303343887558885
66-67	18.279017684763303	25.08147347285505	32.923005637500964	23.716503204880684
68-69	19.027411488962425	24.840624143270304	32.815402021013284	23.316562346753983
70-71	19.0103482894432	24.162869719669473	32.71488145802765	24.111900532859682
72-73	19.28380293382861	23.883789805351014	32.23427382142954	24.59813343939084
74-75	19.681442582438798	23.406826781990887	32.2117538033825	24.699976832187815
76-77	19.617344968723454	23.734651324426597	31.973897598270135	24.674106108579814
78-79	19.418101783921536	23.32689782994826	32.44343192524519	24.81156846088501
80-81	20.254845934048962	23.002934589543596	31.88122634952506	24.860993126882384
82-83	20.17375859139702	22.694416557263107	31.726002007877057	25.405822843462815
84-85	20.63441192370067	23.312997142636497	30.869179087188197	25.18341184647463
86-87	20.512780909722757	23.828094833577882	30.381110510464126	25.278013746235228
88-89	20.568860194459678	23.158695467498667	30.321576683373618	25.950867654668038
90-91	20.791276344343448	22.83086332990957	30.3501509804073	26.027709345339687
92-93	20.445366015646115	23.3463846349883	29.947331433558062	26.260917915807518
94-95	20.59734342420264	22.637269287203647	30.179164414240482	26.586222874353233
96-97	20.68924241254151	22.409452467372	30.50274152444204	26.398563595644454
98-99	20.817051509769094	22.680129739748242	29.755193451231754	26.74762529925091
100	21.247200556027494	22.940767626843773	29.817746544134682	25.994285272994055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	764.0
1	442.0
2	327.5
3	569.5
4	374.5
5	178.5
6	357.0
7	734.5
8	1170.5
9	1354.5
10	915.5
11	421.0
12	328.0
13	316.5
14	292.5
15	271.5
16	282.0
17	290.0
18	302.5
19	293.0
20	331.5
21	404.0
22	495.0
23	600.5
24	637.0
25	740.0
26	981.0
27	1296.5
28	1664.0
29	1956.5
30	2151.5
31	2351.0
32	2582.0
33	2814.5
34	3101.0
35	3350.5
36	3696.5
37	3868.5
38	3533.5
39	3372.0
40	3592.5
41	3669.5
42	3295.0
43	3072.5
44	3397.5
45	3709.5
46	3753.0
47	3606.0
48	3641.0
49	3794.5
50	3971.5
51	3959.0
52	3686.0
53	3472.5
54	3358.0
55	3418.5
56	2991.0
57	2641.5
58	2709.5
59	2287.5
60	1982.5
61	1976.0
62	1661.0
63	1298.5
64	969.5
65	810.5
66	789.5
67	724.0
68	588.0
69	342.0
70	241.0
71	148.5
72	49.5
73	43.5
74	31.5
75	41.5
76	51.5
77	36.0
78	21.5
79	35.5
80	33.5
81	23.5
82	24.5
83	8.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04247432234149355
2	0.23631168429994595
3	7.722604062089737E-4
4	0.0030890416248358947
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.722604062089737E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	7.722604062089737E-4
20-21	0.0
22-23	0.0
24-25	7.722604062089737E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.8613020310448683E-4
70-71	0.0
72-73	3.8613020310448683E-4
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.002316781218626921
90-91	0.002316781218626921
92-93	7.722604062089737E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	129490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.927407521816356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52883834281073	31.75380338250058
2	7.854628447642257	6.272299019229284
3	3.0134230784108933	3.6095451386207427
4	1.8200456462032415	2.906788168970577
5	1.1914432710533442	2.378562051123639
6	0.8974507755986229	2.1499729708857824
7	0.738849560945418	2.0650243262027956
8	0.5705775405206762	1.8225345586531778
9	0.5512359289776024	1.9808479419260177
>10	3.5898031023944914	27.165032048806857
>50	0.17987698735058605	4.743223414935517
>100	0.04448570654906967	3.0149046258398333
>500	0.01160496692584426	3.374777975133215
>1k	0.007736644617229508	6.762684377171982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2934	2.265812031817129	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2908	2.2457332612556953	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1658	1.2804077534944784	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	1257	0.97073133060468	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.6880840219321955	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.6216696269982238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.617808324967179	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.5035137848482508	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.4888408371302803	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	589	0.45486137925708553	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	440	0.3397945787319484	RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	253	0.19538188277087032	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	237	0.18302571627152675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	233	0.17993667464669086	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.17221407058460111	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	194	0.1498185188045409	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT	186	0.1436404355548691	TruSeq Adapter, Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	180	0.13900687311761525	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	172	0.13282878986794347	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.1258784462120627	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT	158	0.12201714418101785	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	147	0.11352227971271912	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	143	0.11043323808788323	No Hit
GTACAGAGAAGCACCTATTGATAAAAAGGGGAATTTCAATTACATCGAGT	138	0.10657193605683836	No Hit
GTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAATGATACCAGTGGAAG	133	0.1027106340257935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.722604062089737E-4	0.0
8	0.0	0.0	0.0	7.722604062089737E-4	0.0
9	0.0	0.0	0.0	7.722604062089737E-4	0.0
10-11	0.0	0.0	0.0	7.722604062089737E-4	0.0
12-13	0.0	0.0	0.0	7.722604062089737E-4	0.0
14-15	0.0	0.0	0.0	7.722604062089737E-4	0.0
16-17	0.0	0.0	0.0	0.0011583906093134604	0.0
18-19	0.0	0.0	0.0	0.0023167812186269208	0.0
20-21	0.0	0.0	0.0	0.0027029114217314077	0.0
22-23	0.0	0.0	0.0	0.007722604062089736	0.0
24-25	0.0	0.0	0.0	0.01930651015522434	0.0
26-27	0.0	0.0	0.0	0.04749401498185188	0.0
28-29	0.0	0.0	0.0	0.088037686307823	0.0
30-31	0.0	0.0	0.0	0.18881766931809407	0.0
32-33	0.0	0.0	0.0	0.33014132365433624	0.0
34-35	0.0	0.0	0.0	0.5540968414549386	0.0
36-37	0.0	0.0	0.0	0.91049501892038	0.0
38-39	0.0	0.0	0.0	1.311684299945942	0.0
40-41	0.0	0.0	0.0	1.7229129662522202	0.0
42-43	0.0	0.0	0.0	2.044945555641362	0.0
44-45	0.0	0.0	0.0	2.33994903081319	0.0
46-47	0.0	0.0	0.0	2.7002085103096762	0.0
48-49	0.0	0.0	0.0	3.073596416711715	0.0
50-51	0.0	0.0	0.0	3.3894509228511853	0.0
52-53	0.0	0.0	0.0	3.7068499498030736	0.0
54-55	0.0	0.0	0.0	4.059000695034365	0.0
56-57	0.0	0.0	0.0	4.444358637732643	0.0
58-59	0.0	0.0	0.0	4.820835585759518	0.0
60-61	0.0	0.0	0.0	5.166422117538033	0.0
62-63	0.0	0.0	0.0	5.536720982315236	0.0
64-65	0.0	0.0	0.0	5.947949648621515	0.0
66-67	0.0	0.0	0.0	6.403969418487915	0.0
68-69	0.0	0.0	0.0	6.92678971349139	0.0
70-71	0.0	0.0	0.0	7.482044945555641	0.0
72-73	0.0	0.0	0.0	8.031508224573326	0.0
74-75	0.0	0.0	0.0	8.579813112981697	0.0
76-77	0.0	0.0	0.0	9.17290910495019	0.0
78-79	0.0	0.0	0.0	9.827785929415398	0.0
80-81	0.0	0.0	0.0	10.505058305660668	0.0
82-83	0.0	0.0	0.0	11.184261332921462	0.0
84-85	0.0	0.0	0.0	11.89937446907097	0.0
86-87	0.0	0.0	0.0	12.658120318171287	0.0
88	0.0	0.0	0.0	13.230365279172137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1295	0.0	60.08887	1
ATGTGAG	25	0.005227025	56.39606	5
CTAATAC	25	0.005227025	56.39606	3
CACAACC	35	2.928773E-4	53.710537	94
TAATACA	45	0.0010109686	41.774857	4
GTATCAA	2190	0.0	35.962692	1
GTACACG	90	2.516874E-5	31.440437	1
TACACGG	90	2.5235557E-5	31.428255	2
TATTTGT	60	0.0041488986	31.331146	6
TATCAAC	2635	0.0	29.87772	2
GAGAGAC	95	3.7286736E-5	29.682137	9
ACGGGAG	95	3.7286736E-5	29.682137	5
ATCAACG	2675	0.0	29.515694	3
AGTGAGC	80	4.759699E-4	29.37295	94
TCAACGC	2720	0.0	29.027384	4
CAACGCA	2730	0.0	28.921059	5
AGACTCA	65	0.006136385	28.921055	8
TTAGGAC	65	0.006136385	28.921055	3
GCGACCA	50	5.310368E-5	28.19803	64-65
AACGCAG	2800	0.0	28.030186	6
>>END_MODULE