FastQCFastQC Report
Wed 25 May 2016
SRR1033062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062591
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC46950.031.84374
GGTATCA32000.031.5906071
ATCAACG48200.031.0178783
GTATCAA51750.030.5280021
CAACGCA49800.030.1157265
AACGCAG52000.028.9234566
GTACATG102350.028.4362471
TACATGG104200.026.981462
TATCAAC59400.026.1191772
ACATGGG112550.023.9354253
ACGCAGA61450.023.8642047
GTCGTAC2453.3833203E-1023.0295071
CGCAGAG63900.022.455928
CATGGGG81900.020.723134
ACACCGT6350.019.2443476
GAGTACA75000.018.5566651
TACGACC3701.6552804E-1017.7892784
ACCGTCC4405.456968E-1217.1079988
GCAGAGT81150.016.9319349
GTACGAC3351.657645E-816.841023
Produced by FastQC (version 0.11.5)