FastQCFastQC Report
Wed 25 May 2016
SRR1033062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062591
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48090.11837273306616394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.045.879651
GTATCAA89850.040.3379821
TCAACGC102700.034.413824
ATCAACG103050.034.343813
CAACGCA104950.033.630425
TATCAAC111350.032.2806242
AACGCAG109550.032.2611776
ACGCAGA128750.027.2676757
GTACATG94550.027.0672551
CGCAGAG129750.027.02138
TACATGG97350.025.8412152
ACATGGG103500.023.477493
GCAGAGT151500.022.8628129
TATACCG3200.022.030085
AGAGTAC129900.020.42347110-11
CATGGGG75900.019.9388264
GAGTACT96900.019.93392412-13
CAGAGTA145700.018.88609310-11
AGTACTT107450.017.911112-13
CCTATAC14450.017.5641613
Produced by FastQC (version 0.11.5)