FastQCFastQC Report
Wed 25 May 2016
SRR1033061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506758
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26580.5245107132003837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCG651.4117779E-436.1564337
CGCCGTA1201.6610647E-731.3077394
GTACATG20950.029.6271921
TCTATAC1602.1191227E-929.385813
TACATGG20750.029.003442
CATGGGT2208.731149E-1125.6457984
ACATGGG22250.023.8784483
AGTCTCG1252.41481E-422.5683022
TGTATAC1903.6185884E-722.2713533
GGTATCA13850.021.7285521
CTATACT1754.4160097E-621.4936224
CATGGGG10100.021.4138184
GACTAGT1100.003048936621.3736111
GTTATAC1354.0626E-420.8965783
ATACAGC2501.067383E-820.6814796
CATGGGC6750.020.2000264
TATAGGA1656.829767E-519.9467322
GTGCTAG1908.988454E-619.7987141
GTATCAA17250.019.626551
GTATTAG1708.5844076E-519.3619791
Produced by FastQC (version 0.11.5)