##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033061_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 506758 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.104953449180872 33.0 31.0 34.0 28.0 34.0 2 31.125896384467538 34.0 31.0 34.0 27.0 34.0 3 31.259139470911165 34.0 31.0 34.0 27.0 34.0 4 34.437117914270715 37.0 35.0 37.0 31.0 37.0 5 34.63140789094597 37.0 35.0 37.0 32.0 37.0 6 34.62360140343122 37.0 35.0 37.0 32.0 37.0 7 34.60968154424795 37.0 35.0 37.0 31.0 37.0 8 34.52219797220764 37.0 35.0 37.0 31.0 37.0 9 36.00584105233662 39.0 38.0 39.0 31.0 39.0 10-11 36.08384771429361 39.0 37.5 39.0 31.0 39.0 12-13 36.04806929540332 39.0 37.0 39.0 31.0 39.0 14-15 37.44671519739205 41.0 38.0 41.0 31.0 41.0 16-17 37.510433974401984 41.0 38.0 41.0 31.0 41.0 18-19 37.5484758010727 41.0 38.5 41.0 31.0 41.0 20-21 37.6081551746593 41.0 39.0 41.0 31.0 41.0 22-23 37.590142632183415 40.5 38.0 41.0 31.0 41.0 24-25 37.59152396212788 40.5 38.0 41.0 31.0 41.0 26-27 37.440261426558635 40.0 38.0 41.0 30.5 41.0 28-29 37.371679381479915 40.0 38.0 41.0 30.0 41.0 30-31 37.42490794422584 41.0 38.0 41.0 30.0 41.0 32-33 37.33262129063577 41.0 38.0 41.0 30.0 41.0 34-35 37.22852426602047 41.0 38.0 41.0 30.0 41.0 36-37 37.10622723272252 41.0 38.0 41.0 30.0 41.0 38-39 36.988205415602714 40.5 38.0 41.0 30.0 41.0 40-41 36.902337012933195 40.0 38.0 41.0 30.0 41.0 42-43 36.770969575221315 40.0 38.0 41.0 30.0 41.0 44-45 36.648674515251855 40.0 38.0 41.0 30.0 41.0 46-47 36.52484716570829 40.0 38.0 41.0 29.5 41.0 48-49 36.403816022638026 40.0 37.0 41.0 29.0 41.0 50-51 36.290914006290976 40.0 37.0 41.0 29.0 41.0 52-53 36.145989801838354 40.0 37.0 41.0 29.0 41.0 54-55 36.004229829622815 40.0 36.5 41.0 28.0 41.0 56-57 35.84008441899289 40.0 36.0 41.0 28.0 41.0 58-59 35.65497337979865 40.0 35.5 41.0 28.0 41.0 60-61 35.43562607793069 39.5 35.0 41.0 27.0 41.0 62-63 35.19269947391062 39.0 35.0 41.0 27.0 41.0 64-65 34.92715655204259 39.0 35.0 41.0 26.0 41.0 66-67 34.63279711420441 38.5 35.0 40.5 26.0 41.0 68-69 34.30712391318933 37.5 34.5 40.0 26.0 41.0 70-71 33.9672013071328 37.0 34.0 40.0 25.0 41.0 72-73 33.60944079817191 36.5 34.0 39.0 24.5 41.0 74-75 33.24991317354635 36.0 34.0 39.0 24.0 41.0 76-77 32.87627230354528 35.5 34.0 38.5 23.0 40.0 78-79 32.47502456793973 35.0 33.5 37.0 22.0 39.0 80-81 32.10248678856574 35.0 33.0 37.0 20.5 39.0 82-83 31.855497101180447 35.0 33.0 36.5 20.0 39.0 84-85 31.607785175567038 35.0 33.0 36.0 20.0 37.0 86-87 31.415729006744836 35.0 33.0 36.0 19.5 37.0 88-89 31.163833624728174 35.0 33.0 35.5 17.5 37.0 90-91 30.918156200790122 35.0 33.0 35.0 9.5 36.0 92-93 30.671279979793116 35.0 33.0 35.0 2.0 36.0 94-95 30.44465208245356 35.0 33.0 35.0 2.0 36.0 96-97 30.244750946211013 35.0 32.0 35.0 2.0 36.0 98-99 30.047548336681412 35.0 32.0 35.0 2.0 35.0 100 29.894243406122843 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.018394103125686456 1101 2 -0.07787170802021492 1101 3 0.07729638837388464 1101 4 -0.15517749488780908 1101 5 -0.28328518600791597 1101 6 -0.23536968875012576 1101 7 -0.048173463408424766 1101 8 0.059303525186699346 1101 9 0.08981175231187422 1101 10-11 -0.015813469747719466 1101 12-13 -0.1673173202366911 1101 14-15 0.019568446998576405 1101 16-17 -0.03106589920952274 1101 18-19 -0.006951672341692472 1101 20-21 0.10531739264456519 1101 22-23 -0.03002708882411298 1101 24-25 0.06662831629267174 1101 26-27 -0.06753180076476895 1101 28-29 -0.07970486096659357 1101 30-31 -0.05952857399835665 1101 32-33 -0.0026715335144587016 1101 34-35 -0.12123133313649959 1101 36-37 -0.23456326244028247 1101 38-39 -0.18561138599036298 1101 40-41 -0.18974537780812994 1101 42-43 -0.10791529813491252 1101 44-45 -0.18332341274432906 1101 46-47 -0.14007759627148175 1101 48-49 -0.17865614976373223 1101 50-51 -0.13480987259405453 1101 52-53 -0.10349507839433159 1101 54-55 -0.19219929404771108 1101 56-57 -0.11720474692491933 1101 58-59 -0.05289418227557263 1101 60-61 -0.10527048589865728 1101 62-63 -0.011214253450539502 1101 64-65 0.004163233652128895 1101 66-67 -0.0566990369020246 1101 68-69 -0.012881562227661902 1101 70-71 -0.08359244043729319 1101 72-73 -0.2949054243617013 1101 74-75 -0.20267933059685816 1101 76-77 -0.24588267444367062 1101 78-79 -0.10840891514801854 1101 80-81 -0.06561210974191312 1101 82-83 0.09583819738542942 1101 84-85 0.07302751380729688 1101 86-87 -0.07053815064549696 1101 88-89 -0.0025968421033297773 1101 90-91 0.08387975637056755 1101 92-93 0.014166439842124845 1101 94-95 -0.009728238945463374 1101 96-97 -0.025568254488501196 1101 98-99 -0.01816857712490716 1101 100 -0.038898434753630085 1102 1 0.2269255952222693 1102 2 0.37297778873731957 1102 3 0.38674404431058207 1102 4 0.3675246579531404 1102 5 0.44429876109538924 1102 6 0.5102116565553914 1102 7 0.5829043264328249 1102 8 0.5887106869774428 1102 9 0.7021341013635833 1102 10-11 0.4874962420004749 1102 12-13 0.4504360371583829 1102 14-15 0.6902013881060043 1102 16-17 0.6514964314535518 1102 18-19 0.5740256157242669 1102 20-21 0.38623085841527427 1102 22-23 0.5163846799093221 1102 24-25 0.4889644079229143 1102 26-27 0.4413287389615519 1102 28-29 0.4243946822516236 1102 30-31 0.6425227870548866 1102 32-33 0.627590749220758 1102 34-35 0.5665028042110336 1102 36-37 0.8483421436260201 1102 38-39 0.7213547808572542 1102 40-41 0.5212765614115256 1102 42-43 0.8117554179463511 1102 44-45 0.7585701776388021 1102 46-47 0.593584140673066 1102 48-49 0.4157323788511462 1102 50-51 0.4398377598677783 1102 52-53 0.4520608847110026 1102 54-55 0.5965953693324764 1102 56-57 0.45353896621220713 1102 58-59 0.3820633184335591 1102 60-61 0.26063667100147825 1102 62-63 0.320099263182378 1102 64-65 0.2518059657354357 1102 66-67 0.2680072827278508 1102 68-69 0.2568654471720677 1102 70-71 0.2563811014311099 1102 72-73 0.29747673669545094 1102 74-75 0.3513043054644527 1102 76-77 0.2878245450561181 1102 78-79 -0.0171625953225103 1102 80-81 0.2003532452665553 1102 82-83 0.06065034954169235 1102 84-85 0.120647260083107 1102 86-87 0.10993711760376712 1102 88-89 0.14040422404688968 1102 90-91 0.052375750971346946 1102 92-93 0.10730664348659857 1102 94-95 0.29127091009059924 1102 96-97 0.21878066028795118 1102 98-99 0.19495265100189485 1102 100 0.022302500328581942 1103 1 -0.24380655921832073 1103 2 -0.3271237426951217 1103 3 -0.13560697241389974 1103 4 -0.5558614153187094 1103 5 -0.397171188420927 1103 6 -0.2814796878899912 1103 7 -0.1815286487201817 1103 8 -0.22988648706435555 1103 9 -0.16480331578959095 1103 10-11 -0.08248641304648174 1103 12-13 -0.16746028796782753 1103 14-15 -0.28677071895324957 1103 16-17 -0.2908033965070729 1103 18-19 -0.30249735576231984 1103 20-21 -0.19741270985051074 1103 22-23 -0.23821948852004482 1103 24-25 -0.34736903822312826 1103 26-27 -0.2971960939346303 1103 28-29 -0.1158547748372527 1103 30-31 -0.42000717881927585 1103 32-33 -0.2755771724872318 1103 34-35 -0.34648687342666307 1103 36-37 -0.30255894783688575 1103 38-39 -0.3777088737102545 1103 40-41 -0.5054529983368994 1103 42-43 -0.4540982921814063 1103 44-45 -0.4404561077209337 1103 46-47 -0.45533074214273483 1103 48-49 -0.4998837195215273 1103 50-51 -0.4395020037896131 1103 52-53 -0.5690456668867085 1103 54-55 -0.45570741996159825 1103 56-57 -0.34853467227949153 1103 58-59 -0.5410336722875897 1103 60-61 -0.42656895835238373 1103 62-63 -0.4390597181189193 1103 64-65 -0.3812777694931597 1103 66-67 -0.47690909485626065 1103 68-69 -0.5029598341546091 1103 70-71 -0.5997791772162699 1103 72-73 -0.4243377146117666 1103 74-75 -0.5643435214808648 1103 76-77 -0.4218131851523026 1103 78-79 -0.517409373109686 1103 80-81 -0.34929844551383127 1103 82-83 -0.3889223723467943 1103 84-85 -0.3312945285622959 1103 86-87 -0.41387341346203144 1103 88-89 -0.5193949693422866 1103 90-91 -0.4864917505093196 1103 92-93 -0.2904815224608832 1103 94-95 -0.46879762362629407 1103 96-97 -0.3266687573164724 1103 98-99 -0.17643734251137388 1103 100 -0.23877429691879115 1104 1 -0.11451230798176582 1104 2 0.020951983678877184 1104 3 0.0151080144026281 1104 4 -0.11012535419359182 1104 5 -0.2545972358814268 1104 6 -0.0386856900583723 1104 7 -0.12817889218136713 1104 8 -0.028856187533314426 1104 9 -0.06111258527771213 1104 10-11 0.006590230717513634 1104 12-13 0.07927912836684925 1104 14-15 -0.05651224592644155 1104 16-17 0.09428869533456918 1104 18-19 0.2784382537246515 1104 20-21 0.13852524122629006 1104 22-23 -0.023794012022548827 1104 24-25 -0.1084017755737321 1104 26-27 -0.166362167184694 1104 28-29 -0.1581862284833946 1104 30-31 -0.10250042670061532 1104 32-33 -0.17116151142113267 1104 34-35 -0.13581114833572627 1104 36-37 0.0031724942616975227 1104 38-39 -0.00699029431752507 1104 40-41 -0.06625414282903108 1104 42-43 0.022578831448122116 1104 44-45 -0.10157031956745044 1104 46-47 -0.015429409681885886 1104 48-49 0.03414952463893428 1104 50-51 0.07648175263371826 1104 52-53 -0.02004549367321573 1104 54-55 0.04973597450968725 1104 56-57 -0.026995894910157858 1104 58-59 -0.03581802148600133 1104 60-61 -0.09201671523761945 1104 62-63 0.0034879316305662655 1104 64-65 -0.07667542277106065 1104 66-67 -0.020369643878886734 1104 68-69 0.0579728242876314 1104 70-71 0.09697210745049034 1104 72-73 0.16641011861571542 1104 74-75 0.053874689031275125 1104 76-77 -0.049176426682485896 1104 78-79 0.06002988989896352 1104 80-81 0.11860573534672625 1104 82-83 0.19960896967287667 1104 84-85 0.24643342360511866 1104 86-87 0.1422541743155108 1104 88-89 0.11243974649135424 1104 90-91 0.13222364688734345 1104 92-93 0.23605863420109685 1104 94-95 0.1877200152813039 1104 96-97 0.22085910118735086 1104 98-99 0.0714439998290608 1104 100 0.02349018084252208 1105 1 -0.07278968018644605 1105 2 0.30733368135547323 1105 3 0.2504611216629513 1105 4 0.2739241821000604 1105 5 0.22188118252277178 1105 6 0.13371040131794842 1105 7 0.03055174029379515 1105 8 0.22889927874973637 1105 9 0.3142875989711982 1105 10-11 0.29295302596842276 1105 12-13 0.3524072410491783 1105 14-15 0.21682379343835834 1105 16-17 0.24693274674120858 1105 18-19 0.19211087565026475 1105 20-21 0.12831892622433827 1105 22-23 0.19938692796523583 1105 24-25 0.21206099560146896 1105 26-27 0.16259276744276008 1105 28-29 0.207071312092161 1105 30-31 0.200385078322455 1105 32-33 0.1671361958141233 1105 34-35 0.19352780399234604 1105 36-37 0.27163270266591155 1105 38-39 0.3217169190470912 1105 40-41 0.4326554856686684 1105 42-43 0.45641934670834416 1105 44-45 0.4301539199083777 1105 46-47 0.41530215398645964 1105 48-49 0.45906212579178884 1105 50-51 0.30684163492134786 1105 52-53 0.35828655628490225 1105 54-55 0.3391579781270053 1105 56-57 0.24640182605422467 1105 58-59 0.348038511092831 1105 60-61 0.4330787420120572 1105 62-63 0.41847576443761625 1105 64-65 0.443054730179405 1105 66-67 0.34812817040378263 1105 68-69 0.4367525632749718 1105 70-71 0.3073352130991296 1105 72-73 0.23174187608366736 1105 74-75 0.20555571159280817 1105 76-77 0.2582357118583687 1105 78-79 0.26801029504758844 1105 80-81 0.27162186607987593 1105 82-83 0.3236029129377407 1105 84-85 0.16854747089301725 1105 86-87 0.07739497748952573 1105 88-89 0.034041438556911885 1105 90-91 0.21258581736334747 1105 92-93 0.08956384032707732 1105 94-95 0.08105362717670062 1105 96-97 0.09317819005063654 1105 98-99 -0.09375524168623528 1105 100 -0.01827622099121129 1106 1 0.24500520235589818 1106 2 0.2780178507688511 1106 3 0.2555042752057304 1106 4 0.2854965714536135 1106 5 0.21932245369468006 1106 6 0.28155653464619945 1106 7 0.30215994804047597 1106 8 0.39293227739701564 1106 9 0.4324692439499529 1106 10-11 0.35646970628926766 1106 12-13 0.40508409436523607 1106 14-15 0.39149371170383773 1106 16-17 0.48184234340323684 1106 18-19 0.3629827105808161 1106 20-21 0.39112876485104664 1106 22-23 0.2663416024560519 1106 24-25 0.19408416324297662 1106 26-27 0.23114307513800014 1106 28-29 0.23001413602951004 1106 30-31 0.21465813641302134 1106 32-33 0.08584654313821716 1106 34-35 0.017965283749241223 1106 36-37 0.20294712337960874 1106 38-39 0.28263676586630027 1106 40-41 0.3479716591973485 1106 42-43 0.44791247834093895 1106 44-45 0.27453252794465044 1106 46-47 0.38957578364713186 1106 48-49 0.31660685115234344 1106 50-51 0.3294948633398178 1106 52-53 0.33544375711559127 1106 54-55 0.19349810044300852 1106 56-57 0.25815902307444105 1106 58-59 0.3405366534899983 1106 60-61 0.3687266167238903 1106 62-63 0.39558819227688957 1106 64-65 0.3428975484010621 1106 66-67 0.20931856040512287 1106 68-69 0.2548989614307118 1106 70-71 0.2502791801766904 1106 72-73 0.23597721054743914 1106 74-75 0.29105402546100123 1106 76-77 0.3444132415323864 1106 78-79 0.36432271526592785 1106 80-81 0.29282330643653864 1106 82-83 0.3318487642914114 1106 84-85 0.3087621907284088 1106 86-87 0.22357974705151307 1106 88-89 0.22586989131664126 1106 90-91 0.30788751111608903 1106 92-93 0.36374887662177713 1106 94-95 0.33696747250982995 1106 96-97 0.2677166471924046 1106 98-99 0.3119833221486168 1106 100 0.3980154321800491 1107 1 0.1034161612600073 1107 2 0.06704068181907985 1107 3 0.11338555374454629 1107 4 0.15112214222986609 1107 5 0.06746857241613924 1107 6 0.11295379492017332 1107 7 0.1361873453007476 1107 8 0.09278615867555828 1107 9 0.1591907051371706 1107 10-11 -0.01033394667877019 1107 12-13 0.07756811263007535 1107 14-15 0.256918369238079 1107 16-17 0.12250901006990489 1107 18-19 0.1399690119506829 1107 20-21 0.2712748835725023 1107 22-23 0.2858941138715778 1107 24-25 0.44523484817448633 1107 26-27 0.4232343993389165 1107 28-29 0.2951556885409232 1107 30-31 0.4292334788787855 1107 32-33 0.2173077303528288 1107 34-35 0.3209241878588287 1107 36-37 0.42231698639330517 1107 38-39 0.2120340261402731 1107 40-41 0.24454700166310062 1107 42-43 0.19114535505327268 1107 44-45 0.10291608958848286 1107 46-47 0.1390369708617527 1107 48-49 0.2890908694171941 1107 50-51 0.32091952087406383 1107 52-53 0.30665836898773335 1107 54-55 0.20379033788593404 1107 56-57 0.27998550709270376 1107 58-59 0.23382432472286752 1107 60-61 0.20983163955493467 1107 62-63 0.2596338543773484 1107 64-65 0.19929024246498983 1107 66-67 0.2097934462498685 1107 68-69 0.28543777421609207 1107 70-71 0.16785908885248801 1107 72-73 0.2387534662551971 1107 74-75 0.33014078345186704 1107 76-77 0.41595662052781535 1107 78-79 0.359592121658622 1107 80-81 0.31153563520366134 1107 82-83 0.2665245633781659 1107 84-85 0.3144334236051165 1107 86-87 0.35056604842137773 1107 88-89 0.25235390958147974 1107 90-91 0.2802436754996478 1107 92-93 0.251237461096661 1107 94-95 0.39473002958745695 1107 96-97 0.368063679155874 1107 98-99 0.5691979340207602 1107 100 0.5826547015864421 1108 1 0.4250265508703954 1108 2 0.5852051839835823 1108 3 0.5877405320995237 1108 4 0.5535983327060592 1108 5 0.7183083992559673 1108 6 0.6219408079071869 1108 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0.2230892077273765 2213 66-67 0.35351258889583903 2213 68-69 0.23025965152577754 2213 70-71 0.311355023811835 2213 72-73 0.2118162896107023 2213 74-75 0.3268739689655433 2213 76-77 0.4152056966549438 2213 78-79 0.36863277206512635 2213 80-81 0.36654228708835745 2213 82-83 0.33257925665237664 2213 84-85 0.40552433269602517 2213 86-87 0.31533323245685096 2213 88-89 0.43077223921932273 2213 90-91 0.41464722317148883 2213 92-93 0.4146373132770016 2213 94-95 0.28005892833098756 2213 96-97 0.16343544560081114 2213 98-99 0.22942705449378664 2213 100 0.292387148001815 2214 1 0.24047945239924573 2214 2 0.12333604046886748 2214 3 0.005166144461846045 2214 4 0.2506748848458997 2214 5 0.2636204711503183 2214 6 0.24777236032101513 2214 7 0.22375696555391045 2214 8 0.15433046247302684 2214 9 0.0511907051371665 2214 10-11 0.19658588454485937 2214 12-13 0.01648372444442714 2214 14-15 0.11738672366846004 2214 16-17 0.12709550796019897 2214 18-19 0.21547534106461086 2214 20-21 0.253359271758157 2214 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88-89 0.471657707049836 2214 90-91 0.44888080630133587 2214 92-93 0.4023767016029929 2214 94-95 0.35844310975623017 2214 96-97 0.3221860420250735 2214 98-99 0.34413464288152085 2214 100 0.38443529230374196 2215 1 -0.3917164183905584 2215 2 -0.3747873414765692 2215 3 -0.4914709039464462 2215 4 -0.3288271554748263 2215 5 -0.4182607869535033 2215 6 -0.3611387025104591 2215 7 -0.4385135796735611 2215 8 -0.28366742131759537 2215 9 -0.3775520149724585 2215 10-11 -0.60121095935434 2215 12-13 -0.6168217469108583 2215 14-15 -0.633704446794809 2215 16-17 -0.7286406987344805 2215 18-19 -0.5467421905159142 2215 20-21 -0.5744092548310604 2215 22-23 -0.5094577189478713 2215 24-25 -0.500894648228396 2215 26-27 -0.36640862909548133 2215 28-29 -0.3257644896022711 2215 30-31 -0.437379059661815 2215 32-33 -0.4535521531689284 2215 34-35 -0.44903059117508093 2215 36-37 -0.5132575254258072 2215 38-39 -0.44248395947123953 2215 40-41 -0.5064807475660871 2215 42-43 -0.40397900260346375 2215 44-45 -0.4578978878010318 2215 46-47 -0.515855800635336 2215 48-49 -0.38145520800658517 2215 50-51 -0.40320586453189833 2215 52-53 -0.38701960292045356 2215 54-55 -0.2760671475542864 2215 56-57 -0.23336838122521186 2215 58-59 -0.3442435067262508 2215 60-61 -0.46411560265129026 2215 62-63 -0.4402380195520763 2215 64-65 -0.5401410696281914 2215 66-67 -0.3658845256583163 2215 68-69 -0.3969664738140537 2215 70-71 -0.2367486535250336 2215 72-73 -0.42178370400858967 2215 74-75 -0.4779818612894786 2215 76-77 -0.3562146710103775 2215 78-79 -0.4506901667963277 2215 80-81 -0.5948014665435153 2215 82-83 -0.44347680695413416 2215 84-85 -0.5408224859529511 2215 86-87 -0.6021266545590933 2215 88-89 -0.6088314278628495 2215 90-91 -0.6000050398138157 2215 92-93 -0.46318871225037483 2215 94-95 -0.5179798025468365 2215 96-97 -0.45662833865844377 2215 98-99 -0.3730185784835207 2215 100 -0.35483621996203496 2216 1 -0.03171670587286002 2216 2 -0.07573387695547851 2216 3 -0.11315856879957664 2216 4 0.06288904199676892 2216 5 0.09902767997525075 2216 6 -0.09863861267223939 2216 7 0.1493661664795667 2216 8 0.3265137644031597 2216 9 0.11928927323573646 2216 10-11 0.12653252018769479 2216 12-13 0.040042971104938374 2216 14-15 0.06379948811920144 2216 16-17 -0.017258555697665656 2216 18-19 0.03678219876386635 2216 20-21 0.25564917594680026 2216 22-23 0.019865475842941294 2216 24-25 -0.039888028948396936 2216 26-27 0.11743486553937998 2216 28-29 0.2546155620008008 2216 30-31 0.15059411823941815 2216 32-33 -0.09367462062952114 2216 34-35 -0.17172516279051564 2216 36-37 -0.05931937724304959 2216 38-39 -0.025530409424163736 2216 40-41 0.12641513353123202 2216 42-43 0.09716733307524805 2216 44-45 0.24491009558249033 2216 46-47 0.20500966483443506 2216 48-49 0.24946782579414162 2216 50-51 0.3111912491457929 2216 52-53 0.1910066873360492 2216 54-55 0.11169976179535723 2216 56-57 0.0281506752578764 2216 58-59 0.13839974929829424 2216 60-61 0.19740273512603324 2216 62-63 0.14233049107398443 2216 64-65 0.10245374562849463 2216 66-67 0.031822084278502416 2216 68-69 0.18746707824539754 2216 70-71 0.27014014113354534 2216 72-73 0.16616072566245776 2216 74-75 0.10480944812053394 2216 76-77 0.13263460920580883 2216 78-79 0.17313924120574598 2216 80-81 0.2570481227161494 2216 82-83 -0.04425732140371608 2216 84-85 0.05262722979892587 2216 86-87 0.19882778668311474 2216 88-89 0.07566393289150497 2216 90-91 0.07061929987527193 2216 92-93 -0.08065398079477859 2216 94-95 -0.008500073642647266 2216 96-97 -0.04958933449713854 2216 98-99 0.10725654207937296 2216 100 0.12383618676792807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9676.0 3 1351.0 4 880.0 5 953.0 6 1933.0 7 3578.0 8 3547.0 9 2331.0 10 1634.0 11 1595.0 12 1683.0 13 1382.0 14 1415.0 15 1356.0 16 1292.0 17 1242.0 18 1190.0 19 1039.0 20 990.0 21 1012.0 22 1156.0 23 1999.0 24 3382.0 25 3927.0 26 3936.0 27 3095.0 28 3302.0 29 4107.0 30 4817.0 31 5993.0 32 8068.0 33 10864.0 34 15223.0 35 23783.0 36 41142.0 37 89895.0 38 165655.0 39 76215.0 40 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00998714584432 21.764593218297527 13.578016431994408 22.64740320386375 2 15.419268360572566 23.351580559893083 38.83559926642596 22.39355181310839 3 17.58131104621891 27.83816174742981 31.19307535721972 23.38745184913156 4 12.011650868878357 19.493285939968406 38.56714060031596 29.92792259083728 5 11.953327434746887 40.29261202047652 35.05932819313657 12.694732351640017 6 28.009629550634134 40.342998431939456 18.01176242895343 13.635609588472978 7 24.227402714360196 36.10885089978355 22.823811479942492 16.83993490591377 8 23.15931683827349 37.583178120179426 21.330928928505827 17.92657611304126 9 23.752688575553663 19.035016166017197 21.698804485008168 35.513490773420976 10-11 21.82371048803395 29.472838007728825 29.067336379698055 19.63611512453917 12-13 22.579307075415795 28.285465966104372 28.270848970884526 20.864377987595304 14-15 20.740073521423337 30.048573127346927 25.950178470193404 23.261174881036332 16-17 19.664198262243286 32.73509083728278 26.862065560821485 20.738645339652447 18-19 19.709719565438515 31.55735640776728 28.758738582688053 19.974185444106155 20-21 21.761511701048793 30.620714988935376 27.70747526135914 19.910298048656692 22-23 21.140137593622004 30.67978435965855 27.72771112644536 20.45236692027408 24-25 21.033548635405925 30.713753509555364 27.605113958778453 20.647583896260258 26-27 20.50947015933668 30.76657735009493 27.878037908311974 20.845914582256412 28-29 20.544666869389737 30.861724436160003 28.057333770372683 20.536274924077578 30-31 21.06966645372207 31.428252646420802 27.397872989574275 20.104207910282852 32-33 20.369090406414156 31.13412852008373 28.01690430111774 20.479876772384376 34-35 20.618418338798826 30.855192844955283 28.015043515074602 20.51134530117129 36-37 20.856288554159185 31.118247068427863 27.48377874934725 20.5416856280657 38-39 20.61807321106805 30.94897933996495 28.03959222965468 20.39335521931232 40-41 20.301054034706624 30.568882525733756 28.254844363259362 20.875219076300265 42-43 20.236782319621575 30.413129955797082 28.2520543941771 21.098033330404245 44-45 20.40430668068658 30.591650252506554 28.26997220700107 20.734070859805794 46-47 20.477883096366508 30.896425750394947 27.982326224328595 20.643364928909953 48-49 20.185184636665493 30.437280996655897 28.055531694948893 21.322002671729724 50-51 20.26378482551085 30.15527150051685 27.85734807295302 21.72359560101928 52-53 20.374734775967767 30.012351615237804 27.942690874911015 21.670222733883413 54-55 20.495227113195753 30.328390803122492 27.68718611444858 21.48919596923317 56-57 20.47763003566083 29.76978425789534 27.658568274892488 22.094017431551343 58-59 20.229021952462002 29.40382025755344 28.042897930331833 22.324259859652727 60-61 20.593246944025967 29.52423356309493 27.514132213944837 22.368387278934264 62-63 20.303392307084746 29.398151804754757 27.620448621751837 22.678007266408656 64-65 20.17926550308961 29.115391184417977 27.846375446608835 22.858967865883578 66-67 20.087195268235035 28.99163687593728 27.73741508284637 23.183752772981315 68-69 20.654239654220895 29.34225556100744 27.50476107734122 22.498743707430442 70-71 20.543788155203323 28.799132845262633 27.680736212792507 22.976342786741537 72-73 20.189757142053292 28.86111163212663 28.0968312274983 22.852299998321783 74-75 20.343253155372153 29.06308769886125 27.892058383639945 22.70160076212665 76-77 20.376306138666642 29.007655440989925 27.846851693723128 22.7691867266203 78-79 20.669868267137858 28.710459164787 28.15825711846086 22.46141544961428 80-81 20.78813279925139 28.49079735222757 28.144624599487898 22.576445249033146 82-83 20.522788071429275 28.437361183775405 28.52975726286487 22.510093481930447 84-85 20.56054840763388 28.38897854636443 28.23381353548215 22.816659510519536 86-87 20.79807567979454 28.53878596872881 28.01467126360996 22.648467087866695 88-89 20.638525734985663 28.14418896176643 28.286423555075192 22.930861748172717 90-91 20.72729821042751 28.034530033647638 28.24723361960308 22.990938136321777 92-93 20.924959901295498 28.189413942011104 28.090709438618138 22.79491671807526 94-95 20.390525757861397 28.148432557170878 28.255425915489063 23.205615769478662 96-97 20.348234643819527 28.253891482662297 28.225760282693884 23.172113590824296 98-99 20.37099367595253 28.47617797352132 27.92332968452805 23.2294986659981 100 20.314338511107074 28.7330906334152 27.67252884364808 23.280042011829646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1855.0 1 1202.5 2 523.5 3 440.5 4 325.0 5 237.0 6 203.0 7 230.0 8 258.0 9 249.0 10 200.0 11 151.0 12 144.0 13 132.0 14 129.0 15 146.5 16 146.0 17 185.5 18 252.0 19 314.5 20 408.5 21 549.0 22 788.5 23 1210.0 24 1735.5 25 2281.5 26 3253.5 27 4571.0 28 6208.5 29 7820.0 30 9057.0 31 10794.0 32 12730.0 33 14686.5 34 16610.0 35 17902.5 36 18809.0 37 19544.0 38 20050.5 39 20136.5 40 20144.5 41 19781.5 42 19008.0 43 18436.0 44 17861.5 45 17149.5 46 16542.5 47 16420.5 48 16556.5 49 15772.5 50 14661.5 51 13742.5 52 12543.0 53 11594.5 54 10878.5 55 10346.5 56 9558.0 57 8653.0 58 7767.0 59 6650.0 60 5590.5 61 4589.5 62 3697.0 63 2974.5 64 2383.0 65 1890.0 66 1528.0 67 1167.5 68 876.5 69 662.5 70 444.5 71 325.5 72 249.0 73 188.0 74 134.5 75 104.5 76 85.0 77 60.5 78 45.0 79 40.0 80 32.0 81 21.0 82 18.5 83 14.5 84 7.0 85 4.5 86 3.0 87 2.5 88 2.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06058118470749352 2 0.03887457129438509 3 0.054661199231191226 4 0.07064516001720743 5 0.08327446236665233 6 0.07893313968403064 7 0.08169579957297171 8 0.09175977488268562 9 0.0886024492953244 10-11 0.09304243840255112 12-13 0.09827175890661814 14-15 0.10083708594634914 16-17 0.07064516001720743 18-19 0.08968777996597982 20-21 0.08179446599757675 22-23 0.05258920431448542 24-25 0.020818615591663082 26-27 0.1735542408802624 28-29 0.06314651174722452 30-31 0.07232248923549307 32-33 0.07459181700140896 34-35 0.01864795425035224 36-37 0.048642547330283885 38-39 0.06817849940208147 40-41 0.07311182063233339 42-43 0.06778383370366133 44-45 0.0662051709099807 46-47 0.07064516001720743 48-49 0.0679811665528714 50-51 0.06363984387024971 52-53 0.06837583225129155 54-55 0.059890519735258246 56-57 0.06314651174722452 58-59 0.05919985476302298 60-61 0.057423859120132294 62-63 0.06275184604880436 64-65 0.05969318688604817 66-67 0.060877183981308626 68-69 0.060679851132098554 70-71 0.054069200683561 72-73 0.05298387001290557 74-75 0.055351864203426485 76-77 0.05041854297317457 78-79 0.047458550235023426 80-81 0.04321589397700678 82-83 0.04795188235804861 84-85 0.039565236266620354 86-87 0.037394574925309516 88-89 0.039565236266620354 90-91 0.03640791067925913 92-93 0.038578572020569975 94-95 0.03640791067925913 96-97 0.04005856838964555 98-99 0.03936790341741028 100 0.046175886715157925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 506758.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.47764714446838 #Duplication Level Percentage of deduplicated Percentage of total 1 89.35558904776994 73.69838743868092 2 8.187273865081876 13.505341698387015 3 1.3948549626310556 3.451330662767845 4 0.41135267063776954 1.3570960168318669 5 0.18826222232946638 0.7763712571961592 6 0.09547716224167437 0.47248390186343864 7 0.06003198617654788 0.3465907881275644 8 0.04609586539654359 0.3041502816796025 9 0.03370491591729803 0.25019119458547945 >10 0.1979224641712999 3.0487497540056236 >50 0.021059074754880263 1.2183977518922318 >100 0.008136455380562922 1.0462859599653924 >500 0.0 0.0 >1k 2.393075111924471E-4 0.5246232940168887 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2658 0.5245107132003837 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 9.86664246050383E-5 0.0 1.973328492100766E-4 0.0 12-13 0.0 1.973328492100766E-4 0.0 5.919985476302298E-4 0.0 14-15 0.0 1.973328492100766E-4 0.0 5.919985476302298E-4 0.0 16-17 0.0 1.973328492100766E-4 0.0 0.0010853306706554212 0.0 18-19 0.0 1.973328492100766E-4 0.0 0.001282663519865498 0.0 20-21 0.0 1.973328492100766E-4 0.0 0.0015786627936806128 0.0 22-23 0.0 1.973328492100766E-4 0.0 0.002466660615125958 0.0 24-25 0.0 1.973328492100766E-4 0.0 0.0039466569842015325 0.0 26-27 0.0 1.973328492100766E-4 0.0 0.007893313968403065 0.0 28-29 0.0 1.973328492100766E-4 0.0 0.01736529073048674 0.0 30-31 0.0 1.973328492100766E-4 0.0 0.04183456403253624 0.0 32-33 0.0 1.973328492100766E-4 0.0 0.0886024492953244 0.0 34-35 0.0 1.973328492100766E-4 0.0 0.17039691529290113 0.0 36-37 0.0 1.973328492100766E-4 0.0 0.2965912723627452 0.0 38-39 0.0 1.973328492100766E-4 0.0 0.4709348446398478 0.0 40-41 0.0 1.973328492100766E-4 0.0 0.6707343544650504 0.0 42-43 0.0 1.973328492100766E-4 0.0 0.8407366040595314 0.0 44-45 0.0 1.973328492100766E-4 0.0 1.0477387628809016 0.0 46-47 0.0 1.973328492100766E-4 0.0 1.2893728367386406 0.0 48-49 0.0 1.973328492100766E-4 0.0 1.5407748866322781 0.0 50-51 0.0 3.946656984201532E-4 0.0 1.787243615295664 0.0 52-53 0.0 3.946656984201532E-4 0.0 2.0287790227287976 0.0 54-55 0.0 3.946656984201532E-4 0.0 2.2991250261466023 0.0 56-57 0.0 3.946656984201532E-4 0.0 2.6283748850536153 0.0 58-59 0.0 3.946656984201532E-4 0.0 2.974990034691115 0.0 60-61 0.0 3.946656984201532E-4 0.0 3.3243678442175555 0.0 62-63 0.0 3.946656984201532E-4 0.0 3.6777909771528026 0.0 64-65 0.0 3.946656984201532E-4 0.0 4.063576697358503 0.0 66-67 0.0 3.946656984201532E-4 0.0 4.516554252720233 0.0 68-69 0.0 3.946656984201532E-4 0.0 5.033960983349054 0.0 70-71 0.0 3.946656984201532E-4 0.0 5.548999719787354 0.0 72-73 0.0 3.946656984201532E-4 0.0 6.082193078352981 0.0 74-75 0.0 3.946656984201532E-4 0.0 6.63363972547054 0.0 76-77 0.0 3.946656984201532E-4 0.0 7.2197182876244685 0.0 78-79 0.0 3.946656984201532E-4 0.0 7.891636639184779 0.0 80-81 0.0 3.946656984201532E-4 0.0 8.597792240083038 0.0 82-83 0.0 3.946656984201532E-4 0.0 9.328811779981766 0.0 84-85 0.0 3.946656984201532E-4 0.0 10.059436654182075 0.0 86-87 0.0 3.946656984201532E-4 0.0 10.861792019070247 0.0 88 0.0 3.946656984201532E-4 0.0 11.470958524581754 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGCG 65 1.4117779E-4 36.156433 7 CGCCGTA 120 1.6610647E-7 31.30773 94 GTACATG 2095 0.0 29.627192 1 TCTATAC 160 2.1191227E-9 29.38581 3 TACATGG 2075 0.0 29.00344 2 CATGGGT 220 8.731149E-11 25.645798 4 ACATGGG 2225 0.0 23.878448 3 AGTCTCG 125 2.41481E-4 22.568302 2 TGTATAC 190 3.6185884E-7 22.271353 3 GGTATCA 1385 0.0 21.728552 1 CTATACT 175 4.4160097E-6 21.493622 4 CATGGGG 1010 0.0 21.413818 4 GACTAGT 110 0.0030489366 21.373611 1 GTTATAC 135 4.0626E-4 20.896578 3 ATACAGC 250 1.067383E-8 20.681479 6 CATGGGC 675 0.0 20.200026 4 TATAGGA 165 6.829767E-5 19.946732 2 GTGCTAG 190 8.988454E-6 19.798714 1 GTATCAA 1725 0.0 19.62655 1 GTATTAG 170 8.5844076E-5 19.361979 1 >>END_MODULE