FastQCFastQC Report
Wed 25 May 2016
SRR1033061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506758
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.5106974137556782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.4953054515172923No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT16410.3238232055537357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.2776473188385778No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT7410.14622364126466678RNA PCR Primer, Index 23 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.1458289755662466No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.10359974583529022No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT250.005235617556.396578
GGTATCA20150.048.175041
GTATCAA31900.032.941561
TCTACGG600.0041160331.3995842
TGCGCAG650.00615279628.9213165
ATCAACG38800.027.0139083
TATCAAC39200.026.6736282
CAACGCA39400.026.5999015
TCAACGC39250.026.5844444
AACGCAG41700.025.2454646
TACATGG18900.024.9203072
GTACATG18700.024.6953071
ATGGGCT4700.023.9985395
CGCAGAG44050.023.4718958
CATGGGC5750.022.8878214
ACGCAGA45900.022.7306427
GCAGAGT44950.022.583729
ACATGGG19850.022.4945533
GAGTACT32050.022.29101412-13
CAGAGTA45250.021.03394310-11
Produced by FastQC (version 0.11.5)