##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033061_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506758
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.618032670426516	34.0	31.0	34.0	31.0	34.0
2	32.59788301319367	34.0	31.0	34.0	31.0	34.0
3	32.68384909562355	34.0	31.0	34.0	31.0	34.0
4	36.225354113797906	37.0	35.0	37.0	35.0	37.0
5	36.222871666554845	37.0	37.0	37.0	35.0	37.0
6	36.30147131372371	37.0	37.0	37.0	35.0	37.0
7	36.24264441804569	37.0	37.0	37.0	35.0	37.0
8	36.252627486887235	37.0	37.0	37.0	35.0	37.0
9	38.041972697026985	39.0	39.0	39.0	35.0	39.0
10-11	38.004242656258015	39.0	38.5	39.0	35.0	39.0
12-13	37.98861685459332	39.0	38.0	39.0	35.0	39.0
14-15	39.40575383121727	41.0	39.0	41.0	36.0	41.0
16-17	39.39536919002759	41.0	39.0	41.0	36.0	41.0
18-19	39.47401619708026	41.0	39.0	41.0	36.5	41.0
20-21	39.48357401363175	41.0	39.0	41.0	36.0	41.0
22-23	39.38831454066833	41.0	39.0	41.0	36.0	41.0
24-25	39.31172867522565	41.0	39.0	41.0	36.0	41.0
26-27	39.170650488004135	41.0	39.0	41.0	35.5	41.0
28-29	39.071729504023615	41.0	39.0	41.0	35.0	41.0
30-31	38.95649402673465	41.0	39.0	41.0	35.0	41.0
32-33	38.77775289191291	41.0	38.0	41.0	35.0	41.0
34-35	38.610471862309026	41.0	38.0	41.0	35.0	41.0
36-37	38.43754415322501	41.0	38.0	41.0	35.0	41.0
38-39	38.25666491698207	41.0	38.0	41.0	34.5	41.0
40-41	38.09959487566057	41.0	38.0	41.0	34.0	41.0
42-43	37.8760433974402	40.0	38.0	41.0	33.5	41.0
44-45	37.56439266868999	40.0	38.0	41.0	33.0	41.0
46-47	37.17035744872305	40.0	38.0	41.0	32.5	41.0
48-49	36.81096993042044	40.0	38.0	41.0	31.0	41.0
50-51	36.58371352795615	40.0	37.0	41.0	30.0	41.0
52-53	36.442764593750866	40.0	37.0	41.0	30.0	41.0
54-55	36.286135591347346	40.0	37.0	41.0	30.0	41.0
56-57	36.12005434546667	40.0	36.0	41.0	29.0	41.0
58-59	35.95037374841641	40.0	36.0	41.0	28.5	41.0
60-61	35.74256647157026	39.5	35.5	41.0	28.0	41.0
62-63	35.50226834110167	39.0	35.0	41.0	28.0	41.0
64-65	35.223904704020455	39.0	35.0	41.0	27.5	41.0
66-67	34.90242679937958	38.5	35.0	41.0	26.5	41.0
68-69	34.54466135709747	37.5	35.0	40.0	26.0	41.0
70-71	34.15011011172986	37.0	35.0	40.0	25.5	41.0
72-73	33.774284767088034	36.5	35.0	39.0	24.0	41.0
74-75	33.43416482818229	36.0	34.0	39.0	24.0	41.0
76-77	33.06721748842643	36.0	34.0	39.0	23.0	40.5
78-79	32.6852106922831	35.0	34.0	37.0	22.0	39.0
80-81	32.34139273578315	35.0	34.0	37.0	21.5	39.0
82-83	32.02597788293426	35.0	34.0	36.5	20.0	39.0
84-85	31.794470930897983	35.0	34.0	36.0	20.0	37.0
86-87	31.57761100959432	35.0	34.0	36.0	19.5	37.0
88-89	31.348556904873725	35.0	34.0	35.5	18.0	37.0
90-91	31.1535969831754	35.0	33.0	35.0	16.5	36.0
92-93	30.971594923020454	35.0	33.0	35.0	9.0	36.0
94-95	30.812149980858713	35.0	33.0	35.0	4.5	36.0
96-97	30.661671843365077	35.0	33.0	35.0	2.0	36.0
98-99	30.531424269572458	35.0	33.0	35.0	2.0	35.0
100	30.419867076592773	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13439864704235305
1101	2	0.1592698863297457
1101	3	0.1380062864254441
1101	4	0.10404438741375799
1101	5	0.07564056244183348
1101	6	0.15452673417396312
1101	7	0.1650631883869096
1101	8	0.13573211622922088
1101	9	0.30480192677584483
1101	10-11	0.14821392140119372
1101	12-13	0.1263563068124114
1101	14-15	0.22712750454789443
1101	16-17	0.2982499490591479
1101	18-19	0.2919595486871174
1101	20-21	0.3661756119394681
1101	22-23	0.2303472889990985
1101	24-25	0.3518232169721358
1101	26-27	0.2978421913705347
1101	28-29	0.3287165662334317
1101	30-31	0.23495269044342848
1101	32-33	0.3751518627734498
1101	34-35	0.2792717175084931
1101	36-37	0.25990644954126907
1101	38-39	0.12321814992647262
1101	40-41	0.4084270631007314
1101	42-43	0.2809038487367559
1101	44-45	0.3534865185266156
1101	46-47	0.059203349550401185
1101	48-49	0.1074852282662988
1101	50-51	0.08072698565847247
1101	52-53	0.1836384944195686
1101	54-55	0.26543754519497753
1101	56-57	0.22733430477839534
1101	58-59	0.13262478230559793
1101	60-61	0.05949894439075365
1101	62-63	-0.0734441480624497
1101	64-65	0.02397559627260648
1101	66-67	0.09971234721355415
1101	68-69	0.20096316825635085
1101	70-71	0.024673616751051952
1101	72-73	0.1211594085010077
1101	74-75	0.10339570078602378
1101	76-77	0.21375185603726976
1101	78-79	0.08513516563053969
1101	80-81	0.15840493061944727
1101	82-83	0.1830773396555827
1101	84-85	0.08327140527737598
1101	86-87	0.07600059190180275
1101	88-89	0.08780062342781747
1101	90-91	0.11670781950572362
1101	92-93	0.23666562746222297
1101	94-95	0.31697760793208474
1101	96-97	0.33496532885935437
1101	98-99	0.22073301104444454
1101	100	0.1107888773182033
1102	1	0.13025875639610973
1102	2	0.08299123965583988
1102	3	0.1331328061785655
1102	4	0.09996562923423369
1102	5	0.09165971402100581
1102	6	0.16425902287570437
1102	7	0.17754643809102788
1102	8	0.17007389073355483
1102	9	0.0603796530393339
1102	10-11	0.23885625245551267
1102	12-13	0.2921108770756291
1102	14-15	0.38140628815853006
1102	16-17	0.3013640369138457
1102	18-19	0.303004962773997
1102	20-21	0.3420331781826107
1102	22-23	0.26579556115105873
1102	24-25	0.365097520795608
1102	26-27	0.269651565667516
1102	28-29	0.23846059236878858
1102	30-31	0.42547324562404043
1102	32-33	0.5349007795061382
1102	34-35	0.30076222485906356
1102	36-37	0.3435173242735061
1102	38-39	0.4551491044732572
1102	40-41	0.49419713655750996
1102	42-43	0.534249017315652
1102	44-45	0.5006189167777322
1102	46-47	0.4895820285022481
1102	48-49	0.37895984117551507
1102	50-51	0.5867301938903182
1102	52-53	0.4182612337409495
1102	54-55	0.31121720422252963
1102	56-57	0.41313298822959155
1102	58-59	0.4556303967113422
1102	60-61	0.3770050820904203
1102	62-63	0.3776608824059693
1102	64-65	0.5051137849909821
1102	66-67	0.5055793523095602
1102	68-69	0.42885400034258225
1102	70-71	0.48592541404556755
1102	72-73	0.5038070800876113
1102	74-75	0.5325321680141286
1102	76-77	0.24315412723067453
1102	78-79	0.2117991913746451
1102	80-81	0.2246150600246537
1102	82-83	0.22180964791971292
1102	84-85	0.1387466735266436
1102	86-87	0.22594019302468382
1102	88-89	0.27933475490663895
1102	90-91	0.27211021785466016
1102	92-93	0.28858420879774727
1102	94-95	0.35299196672586675
1102	96-97	0.38452175168083613
1102	98-99	0.148540458446071
1102	100	0.35849274186821845
1103	1	-0.15399400046431566
1103	2	-0.2164841812765559
1103	3	-0.21463350230776967
1103	4	-0.05879004665487031
1103	5	0.08292378035487502
1103	6	-0.09038745031717355
1103	7	0.033646784990089884
1103	8	0.09903009120172612
1103	9	0.18303526406113235
1103	10-11	0.08503613230998042
1103	12-13	0.14666004931241616
1103	14-15	0.02209783002614074
1103	16-17	0.03517188867795795
1103	18-19	0.048280930848001447
1103	20-21	0.09887244828442476
1103	22-23	0.034709315839840826
1103	24-25	-0.044077677331706866
1103	26-27	0.022411951486958515
1103	28-29	0.08282136739205725
1103	30-31	-0.01797233634849249
1103	32-33	-0.27050435911247206
1103	34-35	-0.1912315986454587
1103	36-37	-0.13505178760848935
1103	38-39	0.06111901168481637
1103	40-41	0.018229767631758875
1103	42-43	-0.13171962075300314
1103	44-45	-0.5505240169166044
1103	46-47	-0.7573120967762534
1103	48-49	-0.9210443893008389
1103	50-51	-1.1289183381343832
1103	52-53	-0.9694090429321989
1103	54-55	-0.9754363479674453
1103	56-57	-0.9696264104786962
1103	58-59	-0.9799570754380227
1103	60-61	-0.9545479257782716
1103	62-63	-1.0375638969041816
1103	64-65	-0.7918691802909166
1103	66-67	-0.7453478270908391
1103	68-69	-0.8222917536693188
1103	70-71	-0.9235987983807519
1103	72-73	-0.852674009683092
1103	74-75	-0.806854271899013
1103	76-77	-0.8169327385504133
1103	78-79	-0.7347487399224164
1103	80-81	-0.6663549882027695
1103	82-83	-0.6090382805570371
1103	84-85	-0.503711092217781
1103	86-87	-0.5703588452470285
1103	88-89	-0.6404095833058463
1103	90-91	-0.7465708606347796
1103	92-93	-0.5747855066322103
1103	94-95	-0.6659464657416301
1103	96-97	-0.5727786403433086
1103	98-99	-0.6478819687192292
1103	100	-0.773907066350187
1104	1	0.1216143317463434
1104	2	0.14654564352120047
1104	3	0.1319923629577957
1104	4	0.07650893908387957
1104	5	0.21225652347926172
1104	6	0.09513061841650483
1104	7	0.18864720198553897
1104	8	0.11036595216903322
1104	9	0.17979553016838423
1104	10-11	0.16582739721756923
1104	12-13	0.23385379822983765
1104	14-15	0.30513830424619925
1104	16-17	0.2398172180081346
1104	18-19	0.29453599092742877
1104	20-21	0.3516358450652177
1104	22-23	0.3485721692434822
1104	24-25	0.30598679658042016
1104	26-27	0.19688926372673166
1104	28-29	0.2396212801437727
1104	30-31	0.2723366893576724
1104	32-33	0.3081955132227705
1104	34-35	0.3474495409710272
1104	36-37	0.22283616250602734
1104	38-39	0.31082230338275707
1104	40-41	0.2118632093839139
1104	42-43	0.10705518245723056
1104	44-45	0.1280077741750958
1104	46-47	-0.01342428779866367
1104	48-49	0.16218790282692908
1104	50-51	0.19940463446580736
1104	52-53	0.16323721020328463
1104	54-55	0.09243768091430127
1104	56-57	0.1309913843210495
1104	58-59	0.09764257043661217
1104	60-61	-0.014851883385873066
1104	62-63	-0.13313283908294693
1104	64-65	0.1340884658018382
1104	66-67	0.22124121426868726
1104	68-69	0.12155178963725888
1104	70-71	0.10357326677318923
1104	72-73	0.06191569886916426
1104	74-75	-0.02957475022486733
1104	76-77	-0.01671846704146418
1104	78-79	-0.11009596882725958
1104	80-81	-0.17046078957179844
1104	82-83	-0.08955496999806911
1104	84-85	-0.1731780437244197
1104	86-87	-0.1234167049697561
1104	88-89	-0.07461090870985032
1104	90-91	-0.02232995273945093
1104	92-93	-0.02465303038491129
1104	94-95	0.022641331046866497
1104	96-97	0.007149764745321363
1104	98-99	0.04450369433561718
1104	100	-0.059908208276194586
1105	1	0.10064258788813873
1105	2	-0.015376514300129429
1105	3	0.04751642773540965
1105	4	0.05102448981102725
1105	5	0.11492096960834175
1105	6	0.17451085762172625
1105	7	0.09842492935685243
1105	8	0.12898782605602577
1105	9	0.23156429977964876
1105	10-11	0.11209476506474658
1105	12-13	0.1548854329315077
1105	14-15	0.09475729103638741
1105	16-17	0.13055224380496355
1105	18-19	0.18627881447228845
1105	20-21	0.11343344584996373
1105	22-23	0.17979539495237162
1105	24-25	0.09707917770903407
1105	26-27	0.20706573279341
1105	28-29	0.29724681712189494
1105	30-31	0.29594979848834413
1105	32-33	0.3739351651395708
1105	34-35	0.40876403555001417
1105	36-37	0.5023234491324615
1105	38-39	0.4378983368977529
1105	40-41	0.2902505738087555
1105	42-43	0.400972978792538
1105	44-45	0.4350896176988286
1105	46-47	0.598685998901388
1105	48-49	0.617618967670218
1105	50-51	0.5497969095418682
1105	52-53	0.40175398660350936
1105	54-55	0.4273080799146598
1105	56-57	0.5362424781745716
1105	58-59	0.5665013215632939
1105	60-61	0.5896049106433452
1105	62-63	0.663171637210425
1105	64-65	0.557097891148203
1105	66-67	0.5255046066372842
1105	68-69	0.5389660969827474
1105	70-71	0.5008176910550404
1105	72-73	0.500126653919196
1105	74-75	0.4787701732799974
1105	76-77	0.4045549197359932
1105	78-79	0.5291184065669512
1105	80-81	0.473955732342084
1105	82-83	0.5444592486054987
1105	84-85	0.470425673885277
1105	86-87	0.4644407747200816
1105	88-89	0.4328247086334258
1105	90-91	0.41602625252895464
1105	92-93	0.5212798640142005
1105	94-95	0.539252583760355
1105	96-97	0.4788438797238044
1105	98-99	0.48071696145076714
1105	100	0.5415431000701219
1106	1	-0.018621229819103746
1106	2	-0.13140524522397357
1106	3	-0.05423257677998805
1106	4	0.07678134388918068
1106	5	0.037015964559472536
1106	6	-0.038136083525600384
1106	7	0.02734828180602733
1106	8	0.04900040059829536
1106	9	0.09019786618657122
1106	10-11	0.09646803844672291
1106	12-13	0.08055750411104157
1106	14-15	0.02995457798363077
1106	16-17	0.08057701184277022
1106	18-19	0.10748501791313458
1106	20-21	0.0923691823180448
1106	22-23	0.014608777313718235
1106	24-25	0.1286346104828624
1106	26-27	0.17129497050942177
1106	28-29	0.10612234820577982
1106	30-31	0.0243101543859936
1106	32-33	0.2191387393579518
1106	34-35	0.14793849779781
1106	36-37	0.234787678370175
1106	38-39	0.2744111876989024
1106	40-41	0.3668752618267419
1106	42-43	0.4189479059173493
1106	44-45	0.4199973091271758
1106	46-47	0.5419405190682838
1106	48-49	0.6390442729839094
1106	50-51	0.5963493892034961
1106	52-53	0.41873723959948705
1106	54-55	0.4395901688128703
1106	56-57	0.4138093042457882
1106	58-59	0.48633385152309927
1106	60-61	0.5033835605947701
1106	62-63	0.5354514398282149
1106	64-65	0.4635375809761513
1106	66-67	0.4026608548511277
1106	68-69	0.2532810702142072
1106	70-71	0.23348292244567403
1106	72-73	0.317311861684928
1106	74-75	0.3663156607340383
1106	76-77	0.32805978760803356
1106	78-79	0.3867841142659998
1106	80-81	0.3884364609034421
1106	82-83	0.3899000244166544
1106	84-85	0.46711533037164443
1106	86-87	0.4662240734416727
1106	88-89	0.6255762471096205
1106	90-91	0.38280850337201855
1106	92-93	0.13211397531794944
1106	94-95	0.32700764691885453
1106	96-97	0.43818333520801644
1106	98-99	0.056165167673260186
1106	100	0.1773348199245639
1107	1	0.06501347337724184
1107	2	-0.13122259682214832
1107	3	-0.028917508286838256
1107	4	0.0096763210581301
1107	5	0.1270433618197444
1107	6	0.0828502178442605
1107	7	0.08115650098410754
1107	8	0.0884889850731767
1107	9	0.17954946436008612
1107	10-11	0.12547260465677113
1107	12-13	0.21560773242153886
1107	14-15	0.23027421268682957
1107	16-17	0.24207472873774094
1107	18-19	0.24069049736519332
1107	20-21	0.20092625994361413
1107	22-23	0.25438046681143334
1107	24-25	0.14842456482076472
1107	26-27	0.13639999334046848
1107	28-29	0.1802593345071486
1107	30-31	0.1742279626051655
1107	32-33	0.17853599963191868
1107	34-35	0.19737228775214533
1107	36-37	0.029737450059670323
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2216	54-55	0.24439016333888475
2216	56-57	0.17287010645728174
2216	58-59	0.11193085725708585
2216	60-61	0.27573449732926747
2216	62-63	0.2051769198276645
2216	64-65	0.1727708134970598
2216	66-67	-0.011968351558905965
2216	68-69	0.14230642841764762
2216	70-71	0.24502480522043157
2216	72-73	0.4048352971535678
2216	74-75	0.1702254495753266
2216	76-77	0.22716068670893463
2216	78-79	0.22265782959177471
2216	80-81	0.35153295345198643
2216	82-83	0.2803540908981219
2216	84-85	0.16032965029829782
2216	86-87	0.2186510984714367
2216	88-89	0.08765457973589719
2216	90-91	0.23555165063571692
2216	92-93	0.0879232345518659
2216	94-95	0.12723344851452012
2216	96-97	0.12611663478515212
2216	98-99	0.10411789987256626
2216	100	-0.03723417272662033
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	0.0
4	5.0
5	22.0
6	45.0
7	161.0
8	206.0
9	331.0
10	621.0
11	1025.0
12	1593.0
13	2118.0
14	2524.0
15	3311.0
16	4436.0
17	6280.0
18	6402.0
19	3109.0
20	1768.0
21	1441.0
22	1525.0
23	1747.0
24	2122.0
25	2600.0
26	3005.0
27	3469.0
28	3539.0
29	4182.0
30	5397.0
31	6756.0
32	8666.0
33	11066.0
34	14993.0
35	23107.0
36	38412.0
37	85997.0
38	169378.0
39	85154.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44145988514075	20.927560494577847	14.2685669048216	22.362412715459804
2	15.828253138116693	22.29892514805423	39.73550017999913	22.137321533829947
3	17.438356569872422	26.972737767516207	32.963719424957326	22.625186237654045
4	11.98746512059296	18.9029612468574	39.20050676275688	29.909066869792756
5	11.362470301288983	38.589380293474576	36.54737191074206	13.500777494494393
6	27.71145201457106	39.810126332490064	18.01135847880053	14.467063174138344
7	23.48517438303885	35.36579590258072	23.631792690001934	17.517237024378502
8	23.91061540708115	35.24372772587883	22.024690480982883	18.82096638605714
9	24.589646340067645	15.517465930483585	22.921789098544078	36.97109863090469
10-11	22.64772917879449	26.97447484484613	29.995362650590522	20.38243332576886
12-13	24.214023261596264	25.555097304827946	29.83396414067464	20.39691529290115
14-15	21.506778896767976	26.947952320295844	28.73776656046839	22.807502222467793
16-17	18.955356270713843	30.155607278841025	29.67588970245078	21.21314674799435
18-19	18.807572085974314	27.53297444924184	33.62150620811594	20.037947256667906
20-21	20.555472237241446	26.74846771042588	33.19227323495633	19.50378681737634
22-23	19.971761669278038	26.98536579590258	33.40499804640479	19.63787448841459
24-25	19.992264445457433	27.112724000544645	33.30787704414988	19.58713450984803
26-27	19.432272832666513	27.14503485394888	33.57039609675239	19.852296216632215
28-29	19.34520755550995	26.91650419531293	33.808183820221174	19.930104428955946
30-31	20.257006302811202	27.50642318424179	33.013292340722785	19.223278172224216
32-33	19.82514336231495	27.175594662541098	33.544907036494735	19.454354938649217
34-35	19.868556589141168	26.959613859080665	33.456699252897835	19.715130298880336
36-37	20.200470441512515	27.144317405941297	32.95576981517805	19.69944233736813
38-39	20.03836150588644	26.839536820336335	33.31817159275236	19.803930081024866
40-41	19.852276629081338	26.699529163821783	33.15359599651116	20.294598210585725
42-43	20.15685988183709	26.53663089679887	32.820004814921525	20.486504406442524
44-45	20.218625063639845	26.9837871331089	32.41912313175125	20.37846467150001
46-47	20.640522695250986	27.26932776591588	31.569999881600292	20.520149657232842
48-49	20.720541165605674	27.150730723540622	31.073115767289316	21.055612343564384
50-51	20.55478157226921	27.219106555791917	30.814313735550304	21.411798136388573
52-53	20.569186870261543	27.384668816279174	30.787969800180758	21.258174513278526
54-55	20.573824192217984	27.827483729906582	30.381168131534185	21.217523946341252
56-57	20.506336357788136	27.41772206852186	30.160352673268108	21.9155889004219
58-59	20.007676247834272	27.342834252246632	30.75432454939044	21.895164950528656
60-61	20.42454189179056	27.83192371901381	29.95986249847067	21.78367189072496
62-63	19.915719140102375	27.98683000564372	30.03859830530549	22.058852548948412
64-65	19.798996759794615	27.70000670931687	30.189459268526598	22.311537262361917
66-67	19.78182880191334	27.68422008138007	29.965091818974738	22.568859297731855
68-69	20.344780974689026	27.9343234867239	29.714863055530614	22.006032483056458
70-71	20.492424391918824	27.443079339645355	29.62666598257946	22.437830285856368
72-73	20.30028228547636	27.364029864441797	29.894239146414503	22.441448703667344
74-75	20.514644578521285	27.610346171492285	29.504644726521068	22.370364523465366
76-77	20.612402764238553	27.528425796928712	29.290509473950088	22.568661964882647
78-79	20.817530260992427	27.299026359721996	29.573090114018918	22.310353265266656
80-81	20.815162266801906	27.29359970636872	29.380493253189886	22.510744773639487
82-83	20.557071182962265	27.317109268239744	29.574401957543795	22.5514175912542
84-85	20.485024410073446	27.34263691939742	29.239794931703102	22.932543738826027
86-87	20.67711087781891	27.419902280387404	28.9698592616565	22.933127580137185
88-89	20.487758672235525	27.273301344952554	29.12629884962205	23.112641133189868
90-91	20.36969247876893	27.053619617737105	29.05728812589357	23.519399777600395
92-93	20.573748655662005	27.16736884687867	28.87499876666239	23.383883730796935
94-95	20.186677980483665	26.881234521613006	29.10222888772681	23.829858610176515
96-97	20.188966309296173	26.921581744307428	29.033442063947813	23.85600988244859
98-99	20.338722169874153	26.940005821324796	28.681075267756274	24.04019674104478
100	20.365736702725957	27.025128365018414	28.328314501201756	24.280820431053876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	337.0
1	216.0
2	221.5
3	340.5
4	207.5
5	121.5
6	227.5
7	470.0
8	787.5
9	885.0
10	619.5
11	321.0
12	256.0
13	274.0
14	325.5
15	363.0
16	381.5
17	434.0
18	495.5
19	578.0
20	677.5
21	844.5
22	1155.0
23	1547.5
24	2022.5
25	2584.5
26	3487.5
27	4692.5
28	6392.0
29	8104.0
30	9291.0
31	10992.5
32	12866.5
33	14795.0
34	16904.0
35	18177.0
36	18989.5
37	19669.0
38	19936.5
39	20089.5
40	20230.5
41	19741.5
42	18760.0
43	18309.0
44	17962.5
45	17340.0
46	16898.5
47	16597.0
48	16425.0
49	15756.0
50	14624.5
51	13620.0
52	12489.5
53	11216.0
54	10164.5
55	9750.0
56	9229.0
57	8404.0
58	7567.5
59	6437.5
60	5252.0
61	4280.5
62	3444.5
63	2711.0
64	2211.0
65	1751.5
66	1338.0
67	994.0
68	680.0
69	488.0
70	344.5
71	236.5
72	176.5
73	130.0
74	87.0
75	60.5
76	43.5
77	24.5
78	11.0
79	16.5
80	14.5
81	11.0
82	11.5
83	4.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04439989107226724
2	0.23679941905209193
3	0.002565327039730996
4	0.0023679941905209194
5	3.9466569842015324E-4
6	0.0
7	0.0
8	7.893313968403065E-4
9	0.0
10-11	5.919985476302298E-4
12-13	0.0
14-15	2.959992738151149E-4
16-17	2.959992738151149E-4
18-19	3.9466569842015324E-4
20-21	0.0
22-23	0.0
24-25	0.0013813299444705362
26-27	9.866642460503831E-5
28-29	3.9466569842015324E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.959992738151149E-4
70-71	0.0
72-73	2.959992738151149E-4
74-75	9.866642460503831E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	9.866642460503831E-5
84-85	0.0
86-87	3.9466569842015324E-4
88-89	0.002269327765915881
90-91	0.0024666606151259574
92-93	5.919985476302298E-4
94-95	5.919985476302298E-4
96-97	1.9733284921007662E-4
98-99	9.866642460503831E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	506758.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.0455603587824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63723813546083	70.06380157072338
2	8.675274572987215	13.714838797761422
3	1.5025623230380314	3.56312642395605
4	0.4351174249588258	1.3757640271096336
5	0.1896232290796732	0.7494437199822265
6	0.11321558116317261	0.5369513432632913
7	0.07974806082709356	0.4412611108922712
8	0.05611022856794689	0.35482115672101777
9	0.040627886269764754	0.28903086327477934
>10	0.22927923760574176	3.557145601276473
>50	0.025720864753151933	1.3949571650206398
>100	0.013734436143088507	1.9551501131477882
>500	7.491510623499285E-4	0.3957673878707953
>1k	9.988680831332381E-4	1.6079407190002486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2588	0.5106974137556782	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2510	0.4953054515172923	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	1641	0.3238232055537357	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1407	0.2776473188385778	No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	741	0.14622364126466678	RNA PCR Primer, Index 23 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	739	0.1458289755662466	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	525	0.10359974583529022	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.973328492100766E-4	0.0
12-13	0.0	0.0	0.0	5.919985476302298E-4	0.0
14-15	0.0	0.0	0.0	5.919985476302298E-4	0.0
16-17	0.0	0.0	0.0	0.0010853306706554212	0.0
18-19	0.0	0.0	0.0	0.0014799963690755745	0.0
20-21	0.0	0.0	0.0	0.0017759956428906893	0.0
22-23	0.0	0.0	0.0	0.002663993464336034	0.0
24-25	0.0	0.0	0.0	0.0039466569842015325	0.0
26-27	0.0	0.0	0.0	0.007893313968403065	0.0
28-29	0.0	0.0	0.0	0.017463957155091782	0.0
30-31	0.0	0.0	0.0	0.04213056330635136	0.0
32-33	0.0	0.0	0.0	0.08909578141834959	0.0
34-35	0.0	0.0	0.0	0.17217291093579185	0.0
36-37	0.0	0.0	0.0	0.29935393225168616	0.0
38-39	0.0	0.0	0.0	0.4724148410089234	0.0
40-41	0.0	0.0	0.0	0.6730036822309663	0.0
42-43	0.0	0.0	0.0	0.8425125997024221	0.0
44-45	0.0	0.0	0.0	1.0518827527143133	0.0
46-47	0.0	0.0	0.0	1.2947008236673125	0.0
48-49	0.0	0.0	0.0	1.5487668670252863	0.0
50-51	0.0	0.0	0.0	1.7970115913315625	0.0
52-53	0.0	0.0	0.0	2.040717660106007	0.0
54-55	0.0	0.0	0.0	2.3147143212341987	0.0
56-57	0.0	0.0	0.0	2.6481081699746234	0.0
58-59	0.0	0.0	0.0	2.997880645199484	0.0
60-61	0.0	0.0	0.0	3.3505144467378907	0.0
62-63	0.0	0.0	0.0	3.707193571685104	0.0
64-65	0.0	0.0	0.0	4.096629949601191	0.0
66-67	0.0	0.0	0.0	4.553455495522518	0.0
68-69	0.0	0.0	0.0	5.075696880956985	0.0
70-71	0.0	0.0	0.0	5.596063604323957	0.0
72-73	0.0	0.0	0.0	6.132808954175365	0.0
74-75	0.0	0.0	0.0	6.688695590400151	0.0
76-77	0.0	0.0	0.0	7.279904806633541	0.0
78-79	0.0	0.0	0.0	7.956953812273314	0.0
80-81	0.0	0.0	0.0	8.666069405909724	0.0
82-83	0.0	0.0	0.0	9.4019236006141	0.0
84-85	0.0	1.973328492100766E-4	0.0	10.138764459564527	0.0
86-87	0.0	1.973328492100766E-4	0.0	10.947138476353604	0.0
88	0.0	1.973328492100766E-4	0.0	11.560547638123127	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTAT	25	0.0052356175	56.39657	8
GGTATCA	2015	0.0	48.17504	1
GTATCAA	3190	0.0	32.94156	1
TCTACGG	60	0.00411603	31.399584	2
TGCGCAG	65	0.006152796	28.921316	5
ATCAACG	3880	0.0	27.013908	3
TATCAAC	3920	0.0	26.673628	2
CAACGCA	3940	0.0	26.599901	5
TCAACGC	3925	0.0	26.584444	4
AACGCAG	4170	0.0	25.245464	6
TACATGG	1890	0.0	24.920307	2
GTACATG	1870	0.0	24.695307	1
ATGGGCT	470	0.0	23.998539	5
CGCAGAG	4405	0.0	23.471895	8
CATGGGC	575	0.0	22.887821	4
ACGCAGA	4590	0.0	22.730642	7
GCAGAGT	4495	0.0	22.58372	9
ACATGGG	1985	0.0	22.494553	3
GAGTACT	3205	0.0	22.291014	12-13
CAGAGTA	4525	0.0	21.033943	10-11
>>END_MODULE