FastQCFastQC Report
Wed 25 May 2016
SRR1033060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819899
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.037.5195961
TCAACGC33150.033.182584
ATCAACG33300.033.1742743
GTATCAA35100.033.0819241
CAACGCA34950.031.4741655
AACGCAG37550.029.4153526
GTACATG68350.028.61251
TATCAAC41850.027.295352
TACATGG69150.026.9883382
ACATGGG72300.025.097293
ACGCAGA45000.024.0232337
CGCAGAG45850.023.2905948
TATACCG2451.9612708E-719.1875485
CATGGGG49250.019.1852594
GCAGAGT56550.018.1372729
ATACCGC2352.7979804E-618.000766
GAGTACA50300.017.6642321
GTATTAG13800.017.033031
CATGGGT17200.016.3983694
AGAGTAC47000.016.32270610-11
Produced by FastQC (version 0.11.5)