##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033060_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2819899
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609864750475104	33.0	31.0	34.0	30.0	34.0
2	31.672049956399146	33.0	31.0	34.0	30.0	34.0
3	31.720796737755503	34.0	31.0	34.0	30.0	34.0
4	35.101873506817086	37.0	35.0	37.0	33.0	37.0
5	35.14073305462359	37.0	35.0	37.0	33.0	37.0
6	35.20644817420766	37.0	35.0	37.0	33.0	37.0
7	35.07647082395504	37.0	35.0	37.0	33.0	37.0
8	35.081876691328304	37.0	35.0	37.0	33.0	37.0
9	36.69272020026249	39.0	37.0	39.0	33.0	39.0
10-11	36.69911918831136	39.0	37.5	39.0	33.0	39.0
12-13	36.66468302588142	39.0	37.0	39.0	33.0	39.0
14-15	38.05937712662758	41.0	38.5	41.0	33.0	41.0
16-17	38.07871328015649	41.0	38.5	41.0	33.0	41.0
18-19	38.095162450853735	41.0	38.5	41.0	33.0	41.0
20-21	38.1199975601963	40.5	39.0	41.0	33.5	41.0
22-23	38.09013230615706	40.0	39.0	41.0	33.0	41.0
24-25	38.10841452122931	40.0	38.0	41.0	33.0	41.0
26-27	38.028749433933626	40.0	38.0	41.0	33.0	41.0
28-29	38.04394909179371	40.0	38.0	41.0	33.0	41.0
30-31	38.198699137806	40.0	39.0	41.0	33.0	41.0
32-33	38.18387697573566	41.0	38.5	41.0	33.0	41.0
34-35	38.141802064542034	40.5	38.0	41.0	33.0	41.0
36-37	38.07242812597188	40.0	38.0	41.0	33.0	41.0
38-39	37.98563601036774	40.0	38.0	41.0	33.0	41.0
40-41	37.9194428594783	40.0	38.0	41.0	33.0	41.0
42-43	37.81149236196048	40.0	38.0	41.0	32.5	41.0
44-45	37.69753491171138	40.0	38.0	41.0	32.0	41.0
46-47	37.57854270667141	40.0	38.0	41.0	32.0	41.0
48-49	37.47379622461656	40.0	38.0	41.0	31.5	41.0
50-51	37.36541929338604	40.0	37.5	41.0	31.0	41.0
52-53	37.21924579568275	40.0	37.0	41.0	31.0	41.0
54-55	37.07844926360838	40.0	37.0	41.0	31.0	41.0
56-57	36.92139505705701	40.0	36.5	41.0	30.5	41.0
58-59	36.73726080260322	40.0	36.0	41.0	30.0	41.0
60-61	36.53118515946848	39.5	35.5	41.0	30.0	41.0
62-63	36.29049728376796	39.0	35.0	41.0	30.0	41.0
64-65	36.02553070163151	39.0	35.0	41.0	30.0	41.0
66-67	35.742257612772654	38.5	35.0	40.5	29.0	41.0
68-69	35.433460382800945	38.0	35.0	40.0	29.0	41.0
70-71	35.107707403704886	37.0	34.5	40.0	29.0	41.0
72-73	34.76129446480175	37.0	34.0	39.5	29.0	41.0
74-75	34.40897688179612	36.0	34.0	39.0	28.0	41.0
76-77	34.03869411634956	36.0	34.0	39.0	28.0	40.0
78-79	33.646919091783076	35.0	34.0	37.5	28.0	39.5
80-81	33.28451302688501	35.0	34.0	37.0	27.0	39.0
82-83	33.03616104690275	35.0	34.0	37.0	27.0	39.0
84-85	32.78869473693916	35.0	34.0	36.0	27.0	38.0
86-87	32.58578693775912	35.0	34.0	36.0	27.0	37.0
88-89	32.34743584787966	35.0	33.5	36.0	27.0	37.0
90-91	32.11865247656033	35.0	33.0	35.0	26.5	36.5
92-93	31.88570955910123	35.0	33.0	35.0	26.0	36.0
94-95	31.672915590239228	35.0	33.0	35.0	25.5	36.0
96-97	31.4881813142953	35.0	33.0	35.0	25.0	36.0
98-99	31.30747129595776	35.0	33.0	35.0	25.0	35.5
100	31.162955836361515	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10308518088043783
1101	2	0.12825281381877573
1101	3	0.14780967209456364
1101	4	0.06048851414871592
1101	5	0.06413909507949711
1101	6	0.1720465505171731
1101	7	0.207245794815627
1101	8	0.2853763953370887
1101	9	0.349618594082159
1101	10-11	0.3557140915174557
1101	12-13	0.2957743540451858
1101	14-15	0.40339920367271276
1101	16-17	0.35815196161466645
1101	18-19	0.4187105285051729
1101	20-21	0.4317102513460114
1101	22-23	0.40817495851198515
1101	24-25	0.3478628369004113
1101	26-27	0.3266195695044942
1101	28-29	0.294891262463743
1101	30-31	0.30349834877120685
1101	32-33	0.2707120095279123
1101	34-35	0.33002377390716475
1101	36-37	0.3119139405381688
1101	38-39	0.2999217881163716
1101	40-41	0.31194781475711153
1101	42-43	0.3190376097876637
1101	44-45	0.3285154078751873
1101	46-47	0.2799428926166527
1101	48-49	0.2983988900446093
1101	50-51	0.30746896078291286
1101	52-53	0.2132845555333276
1101	54-55	0.2759704909255021
1101	56-57	0.2323841960491606
1101	58-59	0.2589602857652764
1101	60-61	0.24830858509727705
1101	62-63	0.29185635594258486
1101	64-65	0.3272603367494966
1101	66-67	0.27723502921043774
1101	68-69	0.23855238359762154
1101	70-71	0.19136692245938036
1101	72-73	0.12005965047458744
1101	74-75	0.13978645291827263
1101	76-77	0.06366699206621007
1101	78-79	0.13367464192626244
1101	80-81	0.1262167782147401
1101	82-83	0.1363603918742058
1101	84-85	0.19507097636611093
1101	86-87	0.2778544275141428
1101	88-89	0.22879297886736083
1101	90-91	0.26769590063273796
1101	92-93	0.30905122019365905
1101	94-95	0.3690103487758023
1101	96-97	0.35460694757511035
1101	98-99	0.31072720294843137
1101	100	0.40202608008407736
1102	1	0.13085331539851808
1102	2	0.20460780778057597
1102	3	0.2234967239047876
1102	4	0.05387241457878389
1102	5	0.11147329334885825
1102	6	0.17055959814327792
1102	7	0.186913883442692
1102	8	0.171889760268769
1102	9	0.3078478202218733
1102	10-11	0.3235000257649219
1102	12-13	0.3520256739772236
1102	14-15	0.43254403383594564
1102	16-17	0.41341177121044836
1102	18-19	0.3820922474594184
1102	20-21	0.4699642217537914
1102	22-23	0.3769302752069521
1102	24-25	0.3748502674941605
1102	26-27	0.2033591225141791
1102	28-29	0.28183239845250085
1102	30-31	0.31021917309769265
1102	32-33	0.2722365027696867
1102	34-35	0.3392330811198434
1102	36-37	0.4311869141402269
1102	38-39	0.40160653459026463
1102	40-41	0.31155031298182934
1102	42-43	0.3136767808129335
1102	44-45	0.27714993273966115
1102	46-47	0.34019302821956643
1102	48-49	0.26165702682767034
1102	50-51	0.33173354770637076
1102	52-53	0.2939707357969894
1102	54-55	0.3524590524422564
1102	56-57	0.34499698826515157
1102	58-59	0.32811442634208987
1102	60-61	0.2557019441560229
1102	62-63	0.3177242754095886
1102	64-65	0.2976771934689779
1102	66-67	0.29897265298831854
1102	68-69	0.3372307293361416
1102	70-71	0.27273294383366675
1102	72-73	0.3024766421881111
1102	74-75	0.30169955502908863
1102	76-77	0.23904164401295702
1102	78-79	0.2139127324425445
1102	80-81	0.3552868049804374
1102	82-83	0.28938271007672256
1102	84-85	0.2373129420463158
1102	86-87	0.2894797800903959
1102	88-89	0.27373374675381257
1102	90-91	0.29516743098151466
1102	92-93	0.34865067757328916
1102	94-95	0.3519892705700727
1102	96-97	0.3929552050560581
1102	98-99	0.3962045234281071
1102	100	0.4159949709166959
1103	1	0.10065139536558121
1103	2	0.16109368538511504
1103	3	0.13574154736484445
1103	4	0.16711154729199507
1103	5	0.00492713614080742
1103	6	0.18039028617244668
1103	7	0.20580429369156406
1103	8	0.23399781931528452
1103	9	0.23241821418015718
1103	10-11	0.23528188654212556
1103	12-13	0.2777619285099604
1103	14-15	0.290721047935385
1103	16-17	0.35247858123319276
1103	18-19	0.3644977159115683
1103	20-21	0.3281396223914683
1103	22-23	0.29052801996389377
1103	24-25	0.22220135657076412
1103	26-27	0.2423928632936665
1103	28-29	0.2484865809588186
1103	30-31	0.29671920412322095
1103	32-33	0.263231436566997
1103	34-35	0.21400777631171053
1103	36-37	0.27447376027897263
1103	38-39	0.2861204883117452
1103	40-41	0.2742152449081985
1103	42-43	0.2702277683221155
1103	44-45	0.2866874413221012
1103	46-47	0.28795038024377817
1103	48-49	0.20552583517658007
1103	50-51	0.20890974626811953
1103	52-53	0.20232567103028032
1103	54-55	0.17971450895142738
1103	56-57	0.20050207608150572
1103	58-59	0.18034408595072904
1103	60-61	0.1382095917966808
1103	62-63	0.21927288944930012
1103	64-65	0.2412213262531182
1103	66-67	0.1524169803895532
1103	68-69	0.1576543181739254
1103	70-71	0.18803981518409074
1103	72-73	0.16885313448057104
1103	74-75	0.20250207746141768
1103	76-77	0.222445273858213
1103	78-79	0.21683269485695433
1103	80-81	0.2631937356659222
1103	82-83	0.2905982518277739
1103	84-85	0.24519513970761864
1103	86-87	0.23183956471744693
1103	88-89	0.29452962172370434
1103	90-91	0.2756772163245458
1103	92-93	0.3026259078428595
1103	94-95	0.26417641277232207
1103	96-97	0.35209781551213837
1103	98-99	0.3785764117074386
1103	100	0.30776294785548686
1104	1	-0.09409245131029564
1104	2	0.021363947653732396
1104	3	-6.761421035825776E-4
1104	4	-0.09155055939347534
1104	5	-0.09210642702678484
1104	6	-0.004032983824899361
1104	7	-0.014392115706918673
1104	8	0.02765502564515998
1104	9	0.03429300384405565
1104	10-11	0.02920625129432608
1104	12-13	0.11496007482185178
1104	14-15	0.11497582268879825
1104	16-17	0.10738915190161435
1104	18-19	0.16320701717464203
1104	20-21	0.08471213202000882
1104	22-23	0.0831317473546207
1104	24-25	0.01393240694649478
1104	26-27	-0.05023407580297601
1104	28-29	-0.03699994502955661
1104	30-31	0.05245865010264339
1104	32-33	0.027948633263058298
1104	34-35	0.08836530602604853
1104	36-37	0.07972483004766673
1104	38-39	0.05766301359572168
1104	40-41	0.16801999583923788
1104	42-43	0.1315285216282973
1104	44-45	0.15328020780000173
1104	46-47	0.08875532128060826
1104	48-49	0.13323166625764316
1104	50-51	0.1347478343243722
1104	52-53	0.1799737254257323
1104	54-55	0.20514919929991038
1104	56-57	0.20966510480884182
1104	58-59	0.23377490717001592
1104	60-61	0.25783853573248194
1104	62-63	0.19938279144155047
1104	64-65	0.1858167746741799
1104	66-67	0.25329927459620194
1104	68-69	0.2693681023655472
1104	70-71	0.21996809216381763
1104	72-73	0.2443616450877144
1104	74-75	0.28606840708516046
1104	76-77	0.24421699075904257
1104	78-79	0.2173961062641112
1104	80-81	0.25677013584494546
1104	82-83	0.2676422583446936
1104	84-85	0.25618447568721336
1104	86-87	0.23073230879573003
1104	88-89	0.26498137587324777
1104	90-91	0.2568704591475708
1104	92-93	0.240214746171155
1104	94-95	0.3203778995875908
1104	96-97	0.3555225972117988
1104	98-99	0.36103581152434217
1104	100	0.3321147130098048
1105	1	-0.012323260833593253
1105	2	0.024559673063073717
1105	3	0.049876205086047065
1105	4	0.012555580676860245
1105	5	0.005340445024245355
1105	6	0.0846390827500656
1105	7	0.07268396907257824
1105	8	0.16492050281271275
1105	9	0.13763458466087286
1105	10-11	0.14818055388808915
1105	12-13	0.1723699439281674
1105	14-15	0.20797801528285476
1105	16-17	0.08561037728240706
1105	18-19	0.13445374022125378
1105	20-21	0.14227602332020695
1105	22-23	0.18597343104503494
1105	24-25	0.04663589659734413
1105	26-27	0.027997549185478476
1105	28-29	0.03303428327070179
1105	30-31	0.057489930532788946
1105	32-33	0.0967833268881293
1105	34-35	0.07631040726173666
1105	36-37	0.17333070348395552
1105	38-39	0.09944179913633633
1105	40-41	0.1810460504968816
1105	42-43	0.14716828164612394
1105	44-45	0.09772646236674376
1105	46-47	0.06272168734763994
1105	48-49	0.012309742859308415
1105	50-51	0.05546174736727494
1105	52-53	0.14627826853134707
1105	54-55	0.11404356992676412
1105	56-57	0.08088073824657016
1105	58-59	0.06927374340295245
1105	60-61	0.05077245738993952
1105	62-63	0.14366819793424668
1105	64-65	0.13546186227631551
1105	66-67	0.1561921493541618
1105	68-69	0.0818597482466501
1105	70-71	-0.008978906302509415
1105	72-73	-0.009359657926090392
1105	74-75	0.03663063208182393
1105	76-77	0.021574344880420426
1105	78-79	0.046758900945697235
1105	80-81	0.07371832304588821
1105	82-83	0.07889975475383437
1105	84-85	0.0785075732946936
1105	86-87	0.040313915105535614
1105	88-89	0.087609575155696
1105	90-91	0.09646254818908773
1105	92-93	0.05793064769912348
1105	94-95	-0.012784119322098775
1105	96-97	-0.06771913645012262
1105	98-99	-0.07064189092200834
1105	100	-0.08816334483130817
1106	1	6.385803195776418E-4
1106	2	0.04456633096701168
1106	3	0.050847357991347764
1106	4	0.010671367086317218
1106	5	-0.01571711161813738
1106	6	-0.006342356203539623
1106	7	0.06250386048902357
1106	8	0.04530850529077668
1106	9	0.12068131903698998
1106	10-11	0.06241734464567372
1106	12-13	0.14186989855453191
1106	14-15	0.14425390582989905
1106	16-17	0.1902073403864577
1106	18-19	0.17862847882518196
1106	20-21	0.15510733160414958
1106	22-23	0.11588345038718018
1106	24-25	0.11456325175227988
1106	26-27	0.14680850854472993
1106	28-29	0.15611758198445358
1106	30-31	0.26086753328107903
1106	32-33	0.18861845371689157
1106	34-35	0.21484836112685457
1106	36-37	0.16747281905708888
1106	38-39	0.17977716936451316
1106	40-41	0.2556153313192766
1106	42-43	0.26142237783159317
1106	44-45	0.22127307272935326
1106	46-47	0.2382422952134604
1106	48-49	0.22512215079871112
1106	50-51	0.22907148208665262
1106	52-53	0.21625576099754795
1106	54-55	0.1767362223261415
1106	56-57	0.16195436862207657
1106	58-59	0.190987567506113
1106	60-61	0.20949833085565928
1106	62-63	0.1974744287148411
1106	64-65	0.21870242559389652
1106	66-67	0.18428328848031583
1106	68-69	0.2658878103978708
1106	70-71	0.2441859118615568
1106	72-73	0.24269157079712045
1106	74-75	0.3012795196187241
1106	76-77	0.2396140921827623
1106	78-79	0.19488556363904053
1106	80-81	0.2753993768504017
1106	82-83	0.27544453660851786
1106	84-85	0.2531370942358606
1106	86-87	0.2069093926087433
1106	88-89	0.23468341822555772
1106	90-91	0.16420002676903067
1106	92-93	0.11954411196230552
1106	94-95	0.09662931747825354
1106	96-97	0.14657110147826558
1106	98-99	0.12984661342509796
1106	100	0.11453205268740874
1107	1	-0.041999128347299575
1107	2	0.014647755545876606
1107	3	-0.004222462463282284
1107	4	-0.010113737404147116
1107	5	0.013597545047531412
1107	6	0.0429359316817326
1107	7	0.04711769083502304
1107	8	0.018540707784275412
1107	9	-0.014849022882444274
1107	10-11	0.045000263718478095
1107	12-13	0.035198803300083625
1107	14-15	0.09192553059430253
1107	16-17	0.06468930764983583
1107	18-19	0.10922111945123447
1107	20-21	0.1141860797682881
1107	22-23	0.12660076199291126
1107	24-25	0.07486191130983855
1107	26-27	0.19434565833685014
1107	28-29	0.18018391847817128
1107	30-31	0.20501560602829727
1107	32-33	0.11590338403458844
1107	34-35	0.17179907346065448
1107	36-37	0.24345814193320336
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2216	50-51	-0.5241281031190823
2216	52-53	-0.5518912640152465
2216	54-55	-0.5581146818293803
2216	56-57	-0.6344243670717162
2216	58-59	-0.6428616946059194
2216	60-61	-0.6391029943510347
2216	62-63	-0.6764120339126336
2216	64-65	-0.7195375872518142
2216	66-67	-0.6791566648015035
2216	68-69	-0.665075630923404
2216	70-71	-0.666213637103489
2216	72-73	-0.6698972199798021
2216	74-75	-0.6484336497853533
2216	76-77	-0.5200070671690185
2216	78-79	-0.5304808686144398
2216	80-81	-0.6208413931024879
2216	82-83	-0.7345431110621377
2216	84-85	-0.5611950165726967
2216	86-87	-0.5887812187687658
2216	88-89	-0.7027758054076685
2216	90-91	-0.6686492752615649
2216	92-93	-0.8273482725171775
2216	94-95	-0.7453178294431524
2216	96-97	-0.7711025969858554
2216	98-99	-0.6951150939054322
2216	100	-0.6933353136156697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31916.0
3	6417.0
4	3351.0
5	2846.0
6	2407.0
7	2589.0
8	3105.0
9	3442.0
10	4003.0
11	4333.0
12	5017.0
13	5645.0
14	6321.0
15	6480.0
16	6331.0
17	6198.0
18	6113.0
19	5970.0
20	6080.0
21	6526.0
22	7509.0
23	10823.0
24	16969.0
25	22393.0
26	23160.0
27	19650.0
28	20803.0
29	26088.0
30	31991.0
31	38397.0
32	49932.0
33	67209.0
34	94589.0
35	143575.0
36	253888.0
37	534015.0
38	913804.0
39	419014.0
40	1000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.217942117097856	20.65653800448299	13.563446545977637	23.562073332441514
2	14.983153934053957	20.720288609278054	41.069775741735995	23.226781714931995
3	17.44298967532207	26.309425264680975	30.754298637556932	25.493286422440022
4	11.726830327723121	17.342968887791145	40.63878133231981	30.291419452165925
5	12.116813294926525	38.67962162952573	36.47235405436485	12.731211021182897
6	29.47712325509585	39.61671558727804	18.42632981838445	12.479831339241665
7	25.936474020650024	33.329949634951	23.696702313810814	17.03687403058816
8	24.154693292780763	37.32586603736346	21.223825771613484	17.295614898242295
9	24.254104444367933	17.437814380046365	22.053306574387584	36.25477460119812
10-11	22.833607995463414	27.773266633476478	29.87702768541025	19.516097685649857
12-13	23.588522878062946	25.17927012564528	29.74071859986127	21.4914883964305
14-15	21.00865902217997	27.299749351101397	26.707358136468663	24.98423349024997
16-17	20.572439790248904	30.615164368432858	27.265903806352153	21.546492034966086
18-19	20.471039121509786	29.549353488591674	29.213878191691954	20.765729198206586
20-21	21.848960362206356	28.883213048060806	28.587142150057172	20.680684439675666
22-23	21.35751914153935	28.67881421272824	28.651191638219142	21.312475007513267
24-25	21.234562996735693	28.75357227605194	28.653795063351257	21.358069663861105
26-27	21.021349445836652	28.671532011734808	28.733541892106008	21.573576650322536
28-29	21.03359582073547	28.633387666563703	28.857279372457402	21.47573714024343
30-31	21.32762376469536	29.187951560939567	28.3959831438543	21.088441530510774
32-33	20.94378421097551	28.830755379758795	28.932338100653304	21.293122308612386
34-35	21.07657348418034	28.59035914453976	28.950019765372875	21.38304760590702
36-37	21.504208178855453	28.98586641580032	28.362254454897013	21.147670950447214
38-39	21.213127797609985	29.046187336464136	28.625576556520727	21.11510830940515
40-41	20.955364156924166	28.796816671307006	28.871115398326886	21.37670377344194
42-43	20.99871675031212	28.666009427058825	28.832093734008176	21.503180088620883
44-45	21.16220857885509	28.838953636821262	28.751441457954318	21.247396326369326
46-47	21.133033763771603	29.06445820621324	28.632405150353506	21.170102879661652
48-49	21.075473924503896	28.82044766757369	28.73197525398556	21.372103153936852
50-51	21.122559604817003	28.708788527769446	28.688756128157678	21.479895739255873
52-53	21.097850961454007	28.691748631000003	28.7372444475674	21.473155959978588
54-55	21.270647457864087	29.159272455779337	28.456915569910468	21.11316451644611
56-57	21.081194157071074	28.864467746591604	28.52972733010185	21.52461076623547
58-59	20.93785895335456	28.490423829765486	28.954834286638924	21.616882930241026
60-61	21.290382674016524	28.90243577892942	28.449202415020963	21.35797913203309
62-63	21.089656501301533	28.910654000783886	28.555155727441623	21.444533770472955
64-65	21.07920394546229	28.61519376279996	28.72991549163832	21.57568680009943
66-67	20.98144638069491	28.709007263999847	28.493718496896197	21.815827858409044
68-69	21.394590357446194	28.873333273008157	28.446550384564134	21.285525984981515
70-71	21.341204326538822	28.620199444306554	28.5541840139759	21.484412215178722
72-73	21.05357185625821	28.73267472525718	28.71456108121246	21.49919233727215
74-75	21.15313171627611	28.7522527301546	28.553441199440645	21.541174354128646
76-77	21.155722552786855	28.762209898488866	28.419498937344105	21.662568611380177
78-79	21.27005690769756	28.836408863330874	28.560163760197536	21.33337046877403
80-81	21.20054202897574	28.598449941878584	28.61583403653179	21.58517399261389
82-83	21.042783993096997	28.694113573112357	28.773180901505917	21.489921532284733
84-85	21.04393328632689	28.575589465235186	28.65307027463293	21.727406973804992
86-87	21.21522912150872	28.75356976880315	28.474710959589327	21.5564901500988
88-89	20.99554521533706	28.625601193713422	28.601211096908685	21.777642494040833
90-91	20.977516506626063	28.537537053881202	28.633018644363624	21.851927795129107
92-93	21.14880584529522	28.68493628880799	28.48568582389615	21.68057204200064
94-95	20.915159047850317	28.429132529321205	28.70758222895632	21.948126193872156
96-97	20.891645605187012	28.436456534356587	28.758604932998246	21.913292927458162
98-99	20.967048990148047	28.537586075998767	28.574712725234413	21.920652208618773
100	21.085219005489108	28.450543216210377	28.506459662280275	21.957778116020236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	494.0
1	454.0
2	391.0
3	349.5
4	340.0
5	348.0
6	328.5
7	325.5
8	301.5
9	268.0
10	275.5
11	261.0
12	265.5
13	282.0
14	322.0
15	405.5
16	520.5
17	751.0
18	1068.5
19	1494.0
20	2275.5
21	3390.0
22	5094.5
23	7899.5
24	11622.5
25	15762.5
26	21438.0
27	29181.5
28	38295.0
29	48005.5
30	56955.0
31	65544.5
32	74568.0
33	83078.0
34	91196.5
35	97856.0
36	101992.5
37	105711.0
38	108031.0
39	108298.5
40	107785.5
41	106594.5
42	103853.5
43	100657.0
44	97446.0
45	93854.0
46	91529.5
47	88731.0
48	85480.0
49	82445.0
50	79274.5
51	75820.5
52	70417.5
53	65611.0
54	62666.0
55	59437.5
56	54820.0
57	49603.0
58	45028.0
59	40167.0
60	34864.5
61	29670.0
62	24608.0
63	19923.0
64	16078.5
65	12845.5
66	9815.5
67	7046.0
68	5142.5
69	3967.5
70	2862.0
71	1951.5
72	1402.5
73	1072.0
74	740.5
75	467.5
76	295.5
77	209.5
78	165.5
79	105.5
80	65.5
81	41.5
82	30.0
83	21.5
84	16.5
85	13.5
86	9.5
87	8.5
88	4.5
89	3.5
90	4.0
91	4.0
92	3.5
93	2.0
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	2.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06000214901313841
2	0.04042697983154716
3	0.0534061680932544
4	0.07099545054627843
5	0.08308808223273245
6	0.08542859159140097
7	0.08755632737200872
8	0.09982627037351337
9	0.09390407245082182
10-11	0.09934752982287663
12-13	0.10358527025258707
14-15	0.10675914279199361
16-17	0.07386789385009888
18-19	0.0958190346533688
20-21	0.08863792639381765
22-23	0.055037432191720345
24-25	0.021915678540259775
26-27	0.17819787162589867
28-29	0.06367249323468678
30-31	0.0770594975210105
32-33	0.07087133262574298
34-35	0.02037307009931916
36-37	0.049629437082675656
38-39	0.07418705421719005
40-41	0.07870849275098152
42-43	0.07280402595979502
44-45	0.07111956846681387
46-47	0.07764462486067764
48-49	0.0734600778254824
50-51	0.06964788455189352
52-53	0.0729636061433406
54-55	0.06439946962639442
56-57	0.06718325727268956
58-59	0.06418669604833364
60-61	0.06042769616925996
62-63	0.066757710116568
64-65	0.06296324797448419
66-67	0.0646654365989704
68-69	0.06519737054412232
70-71	0.057395672681893925
72-73	0.05608356895051915
74-75	0.058583658492733254
76-77	0.05280330962208221
78-79	0.049203889926554105
80-81	0.04363631463396384
82-83	0.050179102159332654
84-85	0.040302861911011706
86-87	0.03861840441803058
88-89	0.040143281727466125
90-91	0.03656159316344309
92-93	0.03847655536599006
94-95	0.03821058839341409
96-97	0.041384460932820645
98-99	0.0414553854588409
100	0.04993086631826175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2819899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.14815650331869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45156854508309	58.765805148314456
2	12.12189901953086	17.491453351570758
3	3.270090106242069	7.0779291829532065
4	1.2490059284124178	3.604539007866879
5	0.6136035478214872	2.2135182399608127
6	0.35573500448896855	1.5399374866547328
7	0.2166815425457767	1.0943221690081244
8	0.14964176738314097	0.8637102122073654
9	0.10746324026849263	0.6977947209520451
>10	0.44410527611589357	5.402492226814746
>50	0.01569288210738481	0.7504598341889513
>100	0.004463999057779552	0.47105770920319145
>500	4.9140942687524606E-5	0.026980710304662463
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.5462263010129084E-5	0.0
5	0.0	0.0	0.0	3.5462263010129084E-5	0.0
6	0.0	0.0	0.0	3.5462263010129084E-5	0.0
7	0.0	0.0	0.0	1.0638678903038726E-4	0.0
8	0.0	0.0	0.0	2.1277357806077452E-4	0.0
9	0.0	0.0	0.0	2.482358410709036E-4	0.0
10-11	0.0	0.0	0.0	2.482358410709036E-4	0.0
12-13	0.0	0.0	0.0	2.6596697257596813E-4	0.0
14-15	0.0	0.0	0.0	3.546226301012909E-4	0.0
16-17	0.0	0.0	0.0	4.610094191316781E-4	0.0
18-19	0.0	0.0	0.0	4.610094191316781E-4	0.0
20-21	0.0	0.0	0.0	5.496650766570008E-4	0.0
22-23	0.0	0.0	0.0	6.20589602677259E-4	0.0
24-25	0.0	1.7731131505064542E-5	0.0	6.915141286975172E-4	0.0
26-27	0.0	3.5462263010129084E-5	0.0	8.15632049232969E-4	0.0
28-29	0.0	3.5462263010129084E-5	0.0	0.001436221651910228	0.0
30-31	0.0	5.319339451519363E-5	0.0	0.0035639574325179732	0.0
32-33	0.0	7.092452602025817E-5	0.0	0.009397499697684207	0.0
34-35	0.0	7.092452602025817E-5	0.0	0.018050291872155705	0.0
36-37	0.0	7.092452602025817E-5	0.0	0.032997635730925116	0.0
38-39	0.0	7.092452602025817E-5	0.0	0.05697012552577238	0.0
40-41	0.0	7.092452602025817E-5	0.0	0.0869180066378264	0.0
42-43	0.0	1.0638678903038726E-4	0.0	0.11762832640459818	0.0
44-45	0.0	1.0638678903038726E-4	0.0	0.15179621681485755	0.0
46-47	0.0	1.0638678903038726E-4	0.0	0.19706379554728734	0.0
48-49	0.0	1.241179205354518E-4	0.0	0.24789894957230738	0.0
50-51	0.0	1.4184905204051634E-4	0.0	0.30509957980764557	0.0
52-53	0.0	1.4184905204051634E-4	0.0	0.36293853077716615	0.0
54-55	0.0	1.4184905204051634E-4	0.0	0.42583085422563005	0.0
56-57	0.0	1.4184905204051634E-4	0.0	0.5005675735194771	0.0
58-59	0.0	1.4184905204051634E-4	0.0	0.5856592736122819	0.0
60-61	0.0	1.4184905204051634E-4	0.0	0.6788895630659112	0.0
62-63	0.0	1.7731131505064544E-4	0.0	0.7733964939879052	0.0
64-65	0.0	1.7731131505064544E-4	0.0	0.8748362973283794	0.0
66-67	0.0	1.7731131505064544E-4	0.0	0.9887765483799242	0.0
68-69	0.0	2.1277357806077452E-4	0.0	1.121990539377474	0.0
70-71	0.0	2.1277357806077452E-4	0.0	1.2668893460368615	0.0
72-73	0.0	2.1277357806077452E-4	0.0	1.4276929776562919	0.0
74-75	0.0	2.1277357806077452E-4	0.0	1.5921137601027555	0.0
76-77	0.0	2.1277357806077452E-4	0.0	1.7606481650583938	0.0
78-79	0.0	2.1277357806077452E-4	0.0	1.9602120501478955	0.0
80-81	0.0	2.1277357806077452E-4	0.0	2.1861953211799428	0.0
82-83	0.0	2.1277357806077452E-4	0.0	2.4261684549694866	0.0
84-85	0.0	2.1277357806077452E-4	0.0	2.657010056033922	0.0
86-87	0.0	2.1277357806077452E-4	0.0	2.898543529395911	0.0
88	0.0	2.1277357806077452E-4	0.0	3.0986570795620696	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2105	0.0	37.519596	1
TCAACGC	3315	0.0	33.18258	4
ATCAACG	3330	0.0	33.174274	3
GTATCAA	3510	0.0	33.081924	1
CAACGCA	3495	0.0	31.474165	5
AACGCAG	3755	0.0	29.415352	6
GTACATG	6835	0.0	28.6125	1
TATCAAC	4185	0.0	27.29535	2
TACATGG	6915	0.0	26.988338	2
ACATGGG	7230	0.0	25.09729	3
ACGCAGA	4500	0.0	24.023233	7
CGCAGAG	4585	0.0	23.290594	8
TATACCG	245	1.9612708E-7	19.187548	5
CATGGGG	4925	0.0	19.185259	4
GCAGAGT	5655	0.0	18.137272	9
ATACCGC	235	2.7979804E-6	18.00076	6
GAGTACA	5030	0.0	17.664232	1
GTATTAG	1380	0.0	17.03303	1
CATGGGT	1720	0.0	16.398369	4
AGAGTAC	4700	0.0	16.322706	10-11
>>END_MODULE