FastQCFastQC Report
Wed 25 May 2016
SRR1033060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819899
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.1872407486934816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44130.15649496666369966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.053.195591
GTATCAA72300.046.163811
TCAACGC87350.037.716164
ATCAACG88400.037.216993
CAACGCA89150.036.9546475
TATCAAC93350.035.435952
AACGCAG93700.035.2103166
ACGCAGA106700.030.7442157
CGCAGAG108050.030.2730988
GCAGAGT119400.026.9230429
GTACATG67150.025.273371
TACATGG69850.023.6789262
AGAGTAC111450.022.81337710-11
ACATGGG71300.022.4781383
GAGTACT88750.021.499212-13
AGTACTT94350.021.02012312-13
CAGAGTA119000.020.87230510-11
GAGTACA52000.020.3979191
GTACTTT98750.019.25067914-15
CATGGGG45450.019.0263864
Produced by FastQC (version 0.11.5)